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The map label for this gene is dksA [H]

Identifier: 50083550

GI number: 50083550

Start: 273643

End: 274182

Strand: Reverse

Name: dksA [H]

Synonym: ACIAD0273

Alternate gene names: 50083550

Gene position: 274182-273643 (Counterclockwise)

Preceding gene: 50083551

Following gene: 50083549

Centisome position: 7.62

GC content: 43.89

Gene sequence:

>540_bases
ATGCCCTATATGGCGAATGACAACCAAAACCACGTCTTAGACGAAAATACAGAAGTTTTAGACGCAGACAAGGCTGCTAA
ACCTGCACGCAAAGCCAAGCCAAAGACCTCTGAAGGAGGTACGACTGCAAGCTTATTTGGTATCGCGCCTTATCAGCCTA
AGAAAAACGAAGAATATATGTCTGAAGGTCAGCTTGAGCATTTCCGCAAGATTTTACAGGCGTGGAAAAGCGAATTGATG
ACTGAAGTTGATCGTACCCTTAATACCATGCAGGACGAATCTTCAGCTTTGCCAGACGTCAATGACCGTGCAACCCAAGA
AGAAGAATTTGCAATCGAATTACGTACCCGTGACCGTGAACGCAAACTGATTCGTAAAATTGAACAGTCTCTTGAAGATA
TTAAGGCAGGCGATTACGGTTATTGCGAAACATGCGGTATCGAAATCGGATTACGTCGTTTAGAAGCACGTCCTACAGCC
ACGCAATGTATTGACTGTAAGACGCTCGCAGAAATCAAAGAGAAGCAAAATAACGGTTAA

Upstream 100 bases:

>100_bases
TGATGACGTCCCCGAGGGAAAATCCGCCAACGGGATTGGATAAAAAACACTTGCATACCACCATATTTTTTGCGTATAGA
TAGTACGTGTATGATGAGGA

Downstream 100 bases:

>100_bases
TACGATGTCTCATCGTCATACTGCTTATGTGGGTCGGTTTGCGCCATCGCCAACCGGCCCCTTGCATTTTGGTTCACTGA
TTACCGCTATTGCAAGCTAT

Product: DnaK suppressor protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 179; Mature: 178

Protein sequence:

>179_residues
MPYMANDNQNHVLDENTEVLDADKAAKPARKAKPKTSEGGTTASLFGIAPYQPKKNEEYMSEGQLEHFRKILQAWKSELM
TEVDRTLNTMQDESSALPDVNDRATQEEEFAIELRTRDRERKLIRKIEQSLEDIKAGDYGYCETCGIEIGLRRLEARPTA
TQCIDCKTLAEIKEKQNNG

Sequences:

>Translated_179_residues
MPYMANDNQNHVLDENTEVLDADKAAKPARKAKPKTSEGGTTASLFGIAPYQPKKNEEYMSEGQLEHFRKILQAWKSELM
TEVDRTLNTMQDESSALPDVNDRATQEEEFAIELRTRDRERKLIRKIEQSLEDIKAGDYGYCETCGIEIGLRRLEARPTA
TQCIDCKTLAEIKEKQNNG
>Mature_178_residues
PYMANDNQNHVLDENTEVLDADKAAKPARKAKPKTSEGGTTASLFGIAPYQPKKNEEYMSEGQLEHFRKILQAWKSELMT
EVDRTLNTMQDESSALPDVNDRATQEEEFAIELRTRDRERKLIRKIEQSLEDIKAGDYGYCETCGIEIGLRRLEARPTAT
QCIDCKTLAEIKEKQNNG

Specific function: Dosage-dependent suppressor of a dnaK deletion mutation. It suppressed not only the temperature-sensitive growth but also the filamentous phenotype of the dnaK deletion strain, while the defect of lambda growth is not suppressed [H]

COG id: COG1734

COG function: function code T; DnaK suppressor protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 dksA C4-type zinc finger [H]

Homologues:

Organism=Escherichia coli, GI1786338, Length=139, Percent_Identity=62.589928057554, Blast_Score=192, Evalue=1e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012784
- InterPro:   IPR020460
- InterPro:   IPR000962
- InterPro:   IPR020458 [H]

Pfam domain/function: PF01258 zf-dskA_traR [H]

EC number: NA

Molecular weight: Translated: 20370; Mature: 20239

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS01102 ZF_DKSA_1 ; PS51128 ZF_DKSA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPYMANDNQNHVLDENTEVLDADKAAKPARKAKPKTSEGGTTASLFGIAPYQPKKNEEYM
CCCCCCCCCCCCCCCCCCHHCCHHHCCCHHHCCCCCCCCCCEEEEEECCCCCCCCCHHHH
SEGQLEHFRKILQAWKSELMTEVDRTLNTMQDESSALPDVNDRATQEEEFAIELRTRDRE
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCHHH
RKLIRKIEQSLEDIKAGDYGYCETCGIEIGLRRLEARPTATQCIDCKTLAEIKEKQNNG
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PYMANDNQNHVLDENTEVLDADKAAKPARKAKPKTSEGGTTASLFGIAPYQPKKNEEYM
CCCCCCCCCCCCCCCCCHHCCHHHCCCHHHCCCCCCCCCCEEEEEECCCCCCCCCHHHH
SEGQLEHFRKILQAWKSELMTEVDRTLNTMQDESSALPDVNDRATQEEEFAIELRTRDRE
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCHHH
RKLIRKIEQSLEDIKAGDYGYCETCGIEIGLRRLEARPTATQCIDCKTLAEIKEKQNNG
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]