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The map label for this gene is gacA [H]

Identifier: 50083538

GI number: 50083538

Start: 260868

End: 261503

Strand: Reverse

Name: gacA [H]

Synonym: ACIAD0260

Alternate gene names: 50083538

Gene position: 261503-260868 (Counterclockwise)

Preceding gene: 50083540

Following gene: 50083537

Centisome position: 7.27

GC content: 43.24

Gene sequence:

>636_bases
GTGATCACTGTATTAGTCGTTGATGACCATGAATTGGTACGTACGGGTATTTGCCGTATGCTTGAGGATCAAGCCGATGT
TGATGTGATTGGGCAAGCAGAATCTGGAGAAGAAGCCATTAATATTGTGCGTCAGAAGCATCCTCAAGTGGTTTTACTAG
ATGTCAATATGCCTGGTATTGGTGGTGTAGAAACGACACGTCGCTTATTGCAGACAGCTCCAGACACCAAAGTGATTGCG
GTGAGCGGTCTAGCAGAAGAACCCTACCCATCGCTCTTACTGAAAGCAGGTGCCAAAGGTTATATCACCAAAGGTGCCCC
CATTGCTGAAATGGTTCGCGCCATTAATAAAGTGATGCAAGGTGGAAAATACTTTAGTGCTGATATTGCCGAGCAGCTTG
CCAGCTCTTACCTTTCCGACACTCAACAATCTCCATTTGATGCTTTATCAGAACGTGAAATGCAGGTCGCCATGATGGTC
GTTAACTGTATTAGTGCTCAGGAAATTGCAGATAAACTCTTTGTTAGTGTTAAAACAGTGAATACTTACCGCTATCGAAT
TTTTGAAAAACTCAGCATCGATAGCGATGTAAAACTCACCCATCTAGCAATACGTTACGGATTAATCAAACCTTAA

Upstream 100 bases:

>100_bases
CTCAACTTATGTGAATAGGATTGCTAATTTTGTCATTACAATGCAAGCGAATTTTGCTTTATGTAACAAAAAACCATCTT
TGTAATTTCAGGAGGCCTGT

Downstream 100 bases:

>100_bases
TCTGCGCTACAAGGATGATTGATGCAATCTCAGCTACCATCCACCATGCTGTCTTATACCATCTATCGTTTGGGCACATG
GTATACCCTGTATCGCTTTA

Product: response regulator

Products: NA

Alternate protein names: Global activator [H]

Number of amino acids: Translated: 211; Mature: 211

Protein sequence:

>211_residues
MITVLVVDDHELVRTGICRMLEDQADVDVIGQAESGEEAINIVRQKHPQVVLLDVNMPGIGGVETTRRLLQTAPDTKVIA
VSGLAEEPYPSLLLKAGAKGYITKGAPIAEMVRAINKVMQGGKYFSADIAEQLASSYLSDTQQSPFDALSEREMQVAMMV
VNCISAQEIADKLFVSVKTVNTYRYRIFEKLSIDSDVKLTHLAIRYGLIKP

Sequences:

>Translated_211_residues
MITVLVVDDHELVRTGICRMLEDQADVDVIGQAESGEEAINIVRQKHPQVVLLDVNMPGIGGVETTRRLLQTAPDTKVIA
VSGLAEEPYPSLLLKAGAKGYITKGAPIAEMVRAINKVMQGGKYFSADIAEQLASSYLSDTQQSPFDALSEREMQVAMMV
VNCISAQEIADKLFVSVKTVNTYRYRIFEKLSIDSDVKLTHLAIRYGLIKP
>Mature_211_residues
MITVLVVDDHELVRTGICRMLEDQADVDVIGQAESGEEAINIVRQKHPQVVLLDVNMPGIGGVETTRRLLQTAPDTKVIA
VSGLAEEPYPSLLLKAGAKGYITKGAPIAEMVRAINKVMQGGKYFSADIAEQLASSYLSDTQQSPFDALSEREMQVAMMV
VNCISAQEIADKLFVSVKTVNTYRYRIFEKLSIDSDVKLTHLAIRYGLIKP

Specific function: Forms part of a two-component regulatory system gacA/gacA(lemA). May be involved in lesion formation, swarming and in the production of extracellular protease, syringomycin and N- acyl-L-homoserine lactone (acyl-HSL) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788222, Length=209, Percent_Identity=48.8038277511962, Blast_Score=210, Evalue=5e-56,
Organism=Escherichia coli, GI1790102, Length=203, Percent_Identity=36.4532019704434, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1786747, Length=207, Percent_Identity=27.536231884058, Blast_Score=101, Evalue=3e-23,
Organism=Escherichia coli, GI1788712, Length=204, Percent_Identity=29.4117647058824, Blast_Score=97, Evalue=8e-22,
Organism=Escherichia coli, GI1788521, Length=201, Percent_Identity=30.8457711442786, Blast_Score=97, Evalue=8e-22,
Organism=Escherichia coli, GI1787473, Length=183, Percent_Identity=27.8688524590164, Blast_Score=80, Evalue=8e-17,
Organism=Escherichia coli, GI1788546, Length=208, Percent_Identity=25.4807692307692, Blast_Score=63, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 23168; Mature: 23168

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITVLVVDDHELVRTGICRMLEDQADVDVIGQAESGEEAINIVRQKHPQVVLLDVNMPGI
CEEEEEECCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHCCCCEEEEECCCCCC
GGVETTRRLLQTAPDTKVIAVSGLAEEPYPSLLLKAGAKGYITKGAPIAEMVRAINKVMQ
CCHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHCCCCCEEECCCCHHHHHHHHHHHHH
GGKYFSADIAEQLASSYLSDTQQSPFDALSEREMQVAMMVVNCISAQEIADKLFVSVKTV
CCCEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEH
NTYRYRIFEKLSIDSDVKLTHLAIRYGLIKP
HHHHHHHHHHHCCCCCCHHHHHHHHHCCCCC
>Mature Secondary Structure
MITVLVVDDHELVRTGICRMLEDQADVDVIGQAESGEEAINIVRQKHPQVVLLDVNMPGI
CEEEEEECCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHCCCCEEEEECCCCCC
GGVETTRRLLQTAPDTKVIAVSGLAEEPYPSLLLKAGAKGYITKGAPIAEMVRAINKVMQ
CCHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHCCCCCEEECCCCHHHHHHHHHHHHH
GGKYFSADIAEQLASSYLSDTQQSPFDALSEREMQVAMMVVNCISAQEIADKLFVSVKTV
CCCEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEH
NTYRYRIFEKLSIDSDVKLTHLAIRYGLIKP
HHHHHHHHHHHCCCCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8002569 [H]