Click here to switch to the map view.

The map label for this gene is pilS [H]

Identifier: 50083537

GI number: 50083537

Start: 259269

End: 260849

Strand: Reverse

Name: pilS [H]

Synonym: ACIAD0259

Alternate gene names: 50083537

Gene position: 260849-259269 (Counterclockwise)

Preceding gene: 50083538

Following gene: 50083536

Centisome position: 7.25

GC content: 38.77

Gene sequence:

>1581_bases
TTGATGCAATCTCAGCTACCATCCACCATGCTGTCTTATACCATCTATCGTTTGGGCACATGGTATACCCTGTATCGCTT
TATTATTGCATTTAGCCTATTACTCATTTTTTATATCACGGGTGGGCGAGTTGGAAGTGATTATCCTCAACCTGATACTT
ACTTTTATACCCTCATTGCCTACACACTTATCAATTTTATACAAGGTGCGCTATTTCGATTTTATCCTAAGTGGATAACG
CAGCAACTCAGTTCACTGTTTATTATCGATATTATCGCTTTAAGTTTTTTAACATATGCCGTGGGGGGTCCCAATTTACA
TTTGGGGCTACTGTATGTCGTGACTGTTTTCTCGGCGGCGATTCTACTACATGCCAGAATGTCGCTGGTGATGACTCTAC
TGGCGGTTATTATTCTGGTTTACCAGCAAATTGTAATTGTTTTATTTAATACAGATCACCTCAATGCGATTGGGGATAGC
ATTCTACTGGCCTTTCTATTTTTCGTGGTATACGGAATCGGGCAGATTGCCGTACGCCGTTTTCAGATTCTGGAAACCAT
TACCAACTATCAGTCCGATATCTTAAATCAACTGCAAAATATCAATCGATATATTCTGGATCAGGTCGATGATGGCTATC
TGGTGTTGGATGAAAATTATTATGTTGTGGTCAGCAATCCAGCAGCCTGTAACTTTCTAGGTATCCCCCCCCTGTTCGCA
CATGAGCAGTTAACCTTGGCAAAAGTTCAACCTGATCTGTTTGAAATTTTAAAGTCTACAAATCTTCAAGATGGTGAAAA
ATTCAATTTTGAATCACAGCTTTCTACTTACCAAATCCATATCCGCATTCAACGCTTACAGATTCCGAAGCAATCATTAA
TTTTATTGACCTTACAAGATACCCAAAAACTCAATCAGCATGTGCAGCAGCTTAAACTGGCCGCACTCGGACAGCTTTCT
GCCAGTATTGCGCATGAAATTCGTAATCCTCTGGCAGCCATTGCTCAAGCCAATGAGTTATTTACAGATAGTGATTCTGC
ACAACAGAAACAATTGCAATATATGATTGCAAAACAGACCAAACGTATTAACAGTATTATTGAAGATACGTTAGGTATGG
CTCGTAATCATGCGACTTATCCTGTTGTGATTCAGTTGACAGCATTTTTATATGGTTTACTTGAAGAAGACCTAACCGAT
GTCAAAGATCAAATTCGTCTGGTCTTTTCCCGTGAAGATCTACAAATAATTTTTGATCCATCACAGTTACGTCAAGTTCT
TATTAATCTGACCCGTAATGCATTACGTCATAATGCACCTGATGCACCCTTCGTCGAAATTTCAGTGAAGGAAAATGGCG
ATATATTGTGGATTGATATTATTGATTATGGCGACGGCGTCACAAAAAGGAATATTTCTCAACTATTTAAGCCTTTTTTT
AGTACCGAAATCAAAGGAACTGGTTTAGGATTATATTTGTCTCATAGTCTGTGCGAGGTCAATCATGCCAAGCTAACGTA
TGTAGAACAAGAAAAAGGAGCCTGTTTTAGAATTCAGTGCTCAATAAAGGAAAACGAATAA

Upstream 100 bases:

>100_bases
TCGAATTTTTGAAAAACTCAGCATCGATAGCGATGTAAAACTCACCCATCTAGCAATACGTTACGGATTAATCAAACCTT
AATCTGCGCTACAAGGATGA

Downstream 100 bases:

>100_bases
TTTAGGTTTTTGGGGTTATGACCGAACAACAACCACGTGTTTTACTCATTGATGATGAAGAAGACCTTTGTACATTGATG
CAAATCAGTCTTTCACGTCT

Product: sensor protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 526; Mature: 526

Protein sequence:

>526_residues
MMQSQLPSTMLSYTIYRLGTWYTLYRFIIAFSLLLIFYITGGRVGSDYPQPDTYFYTLIAYTLINFIQGALFRFYPKWIT
QQLSSLFIIDIIALSFLTYAVGGPNLHLGLLYVVTVFSAAILLHARMSLVMTLLAVIILVYQQIVIVLFNTDHLNAIGDS
ILLAFLFFVVYGIGQIAVRRFQILETITNYQSDILNQLQNINRYILDQVDDGYLVLDENYYVVVSNPAACNFLGIPPLFA
HEQLTLAKVQPDLFEILKSTNLQDGEKFNFESQLSTYQIHIRIQRLQIPKQSLILLTLQDTQKLNQHVQQLKLAALGQLS
ASIAHEIRNPLAAIAQANELFTDSDSAQQKQLQYMIAKQTKRINSIIEDTLGMARNHATYPVVIQLTAFLYGLLEEDLTD
VKDQIRLVFSREDLQIIFDPSQLRQVLINLTRNALRHNAPDAPFVEISVKENGDILWIDIIDYGDGVTKRNISQLFKPFF
STEIKGTGLGLYLSHSLCEVNHAKLTYVEQEKGACFRIQCSIKENE

Sequences:

>Translated_526_residues
MMQSQLPSTMLSYTIYRLGTWYTLYRFIIAFSLLLIFYITGGRVGSDYPQPDTYFYTLIAYTLINFIQGALFRFYPKWIT
QQLSSLFIIDIIALSFLTYAVGGPNLHLGLLYVVTVFSAAILLHARMSLVMTLLAVIILVYQQIVIVLFNTDHLNAIGDS
ILLAFLFFVVYGIGQIAVRRFQILETITNYQSDILNQLQNINRYILDQVDDGYLVLDENYYVVVSNPAACNFLGIPPLFA
HEQLTLAKVQPDLFEILKSTNLQDGEKFNFESQLSTYQIHIRIQRLQIPKQSLILLTLQDTQKLNQHVQQLKLAALGQLS
ASIAHEIRNPLAAIAQANELFTDSDSAQQKQLQYMIAKQTKRINSIIEDTLGMARNHATYPVVIQLTAFLYGLLEEDLTD
VKDQIRLVFSREDLQIIFDPSQLRQVLINLTRNALRHNAPDAPFVEISVKENGDILWIDIIDYGDGVTKRNISQLFKPFF
STEIKGTGLGLYLSHSLCEVNHAKLTYVEQEKGACFRIQCSIKENE
>Mature_526_residues
MMQSQLPSTMLSYTIYRLGTWYTLYRFIIAFSLLLIFYITGGRVGSDYPQPDTYFYTLIAYTLINFIQGALFRFYPKWIT
QQLSSLFIIDIIALSFLTYAVGGPNLHLGLLYVVTVFSAAILLHARMSLVMTLLAVIILVYQQIVIVLFNTDHLNAIGDS
ILLAFLFFVVYGIGQIAVRRFQILETITNYQSDILNQLQNINRYILDQVDDGYLVLDENYYVVVSNPAACNFLGIPPLFA
HEQLTLAKVQPDLFEILKSTNLQDGEKFNFESQLSTYQIHIRIQRLQIPKQSLILLTLQDTQKLNQHVQQLKLAALGQLS
ASIAHEIRNPLAAIAQANELFTDSDSAQQKQLQYMIAKQTKRINSIIEDTLGMARNHATYPVVIQLTAFLYGLLEEDLTD
VKDQIRLVFSREDLQIIFDPSQLRQVLINLTRNALRHNAPDAPFVEISVKENGDILWIDIIDYGDGVTKRNISQLFKPFF
STEIKGTGLGLYLSHSLCEVNHAKLTYVEQEKGACFRIQCSIKENE

Specific function: Member of the two-component regulatory system pilS/pilR involved in the regulation of the expression of type 4 fimbriae. PilS may function as a membrane-associated protein kinase that phosphorylates pilR in response to environmental signals [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=311, Percent_Identity=27.9742765273312, Blast_Score=105, Evalue=9e-24,
Organism=Escherichia coli, GI1788549, Length=352, Percent_Identity=26.4204545454545, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1790300, Length=357, Percent_Identity=24.0896358543417, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI87081816, Length=218, Percent_Identity=27.0642201834862, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1790346, Length=203, Percent_Identity=27.5862068965517, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI87082128, Length=219, Percent_Identity=23.7442922374429, Blast_Score=64, Evalue=3e-11,
Organism=Escherichia coli, GI1789808, Length=201, Percent_Identity=23.3830845771144, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 59938; Mature: 59938

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMQSQLPSTMLSYTIYRLGTWYTLYRFIIAFSLLLIFYITGGRVGSDYPQPDTYFYTLIA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
YTLINFIQGALFRFYPKWITQQLSSLFIIDIIALSFLTYAVGGPNLHLGLLYVVTVFSAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
ILLHARMSLVMTLLAVIILVYQQIVIVLFNTDHLNAIGDSILLAFLFFVVYGIGQIAVRR
HHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FQILETITNYQSDILNQLQNINRYILDQVDDGYLVLDENYYVVVSNPAACNFLGIPPLFA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCEEEEECCCCCCCEECCCCCCC
HEQLTLAKVQPDLFEILKSTNLQDGEKFNFESQLSTYQIHIRIQRLQIPKQSLILLTLQD
CCCCHHHHCCHHHHHHHHHCCCCCCCCCCCHHCCCEEEEEEEEEEECCCCCCEEEEEEHH
TQKLNQHVQQLKLAALGQLSASIAHEIRNPLAAIAQANELFTDSDSAQQKQLQYMIAKQT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
KRINSIIEDTLGMARNHATYPVVIQLTAFLYGLLEEDLTDVKDQIRLVFSREDLQIIFDP
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEECH
SQLRQVLINLTRNALRHNAPDAPFVEISVKENGDILWIDIIDYGDGVTKRNISQLFKPFF
HHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEEEEEECCCCCCHHHHHHHHHHHH
STEIKGTGLGLYLSHSLCEVNHAKLTYVEQEKGACFRIQCSIKENE
CCCCCCCCEEHEEHHHHHHCCCCEEEEEECCCCCEEEEEEEEECCC
>Mature Secondary Structure
MMQSQLPSTMLSYTIYRLGTWYTLYRFIIAFSLLLIFYITGGRVGSDYPQPDTYFYTLIA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
YTLINFIQGALFRFYPKWITQQLSSLFIIDIIALSFLTYAVGGPNLHLGLLYVVTVFSAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
ILLHARMSLVMTLLAVIILVYQQIVIVLFNTDHLNAIGDSILLAFLFFVVYGIGQIAVRR
HHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FQILETITNYQSDILNQLQNINRYILDQVDDGYLVLDENYYVVVSNPAACNFLGIPPLFA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCEEEEECCCCCCCEECCCCCCC
HEQLTLAKVQPDLFEILKSTNLQDGEKFNFESQLSTYQIHIRIQRLQIPKQSLILLTLQD
CCCCHHHHCCHHHHHHHHHCCCCCCCCCCCHHCCCEEEEEEEEEEECCCCCCEEEEEEHH
TQKLNQHVQQLKLAALGQLSASIAHEIRNPLAAIAQANELFTDSDSAQQKQLQYMIAKQT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
KRINSIIEDTLGMARNHATYPVVIQLTAFLYGLLEEDLTDVKDQIRLVFSREDLQIIFDP
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEECH
SQLRQVLINLTRNALRHNAPDAPFVEISVKENGDILWIDIIDYGDGVTKRNISQLFKPFF
HHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEEEEEECCCCCCHHHHHHHHHHHH
STEIKGTGLGLYLSHSLCEVNHAKLTYVEQEKGACFRIQCSIKENE
CCCCCCCCEEHEEHHHHHHCCCCEEEEEECCCCCEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8097014; 10984043 [H]