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The map label for this gene is lutR [H]

Identifier: 50083420

GI number: 50083420

Start: 136994

End: 137707

Strand: Direct

Name: lutR [H]

Synonym: ACIAD0132

Alternate gene names: 50083420

Gene position: 136994-137707 (Clockwise)

Preceding gene: 50083419

Following gene: 50083422

Centisome position: 3.81

GC content: 36.13

Gene sequence:

>714_bases
ATGTCTAGTATTACAAAATCTAGATCAACAAAAAAATTAAGTCAGGTCATTGTTGAGCAATTGATTGAAACGATTCAATC
TGGAGAATTTAAGATTGGAGAAAAAATTCCAACCGAACTTGAATTGATTGAGCAATTTGATGTCAGCCGATCCGTAATTC
GTGAAGCGATTACAGAGCTACGATCTCTTGGGTTTGTTGAGACACGACATGGGATTGGAACTTTTGTTAAAGAGCAAAAC
ATTGAACAAAATTTTCTGTTGTCAAATGCAAGTCTTGAAACAATCAATGATATTGTTGCACTGCTTGAATTGCGCATTAG
TCTTGAGTCTGAGGCCGTATTTCTTGCTTCTGAACGAAGAAAAAACAAGCATCTGGAAAAGATGAAGATAGCACTCAATG
ATTTTGAAGCACATATTTCTTCAGGTGAAAATGATGGAACAGTCAAGGCTGATTATGACTTTCATATCGCGATTGCTGAA
GCTTCAGGTAATCAATATTTTGTCGATTTTCTGAAATATTTGGGTGAAAAAATTATTCCTCGGGCAAGGGTGAAGTCAAT
TGAACAAAGCCCAGAAAATCGGGAAGAATACCTTAAAACAGTACACCATGATCATGTGAATATCTATAATGCAATTCTTG
ATCAGGATGGATTGCTGGCTCGTCAAATGATGCGCGCTCACCTTTCAAAAAGTATCAAAAAATTCAAGCAATAA

Upstream 100 bases:

>100_bases
TTTTATAATTTCATTGAAATGAAAGTTGTGGTGACGCTATAGCCGAATTATCGTAAAATAATTGCTAAATTTTTCAATTT
TATTATGAGCCCTTCAGATT

Downstream 100 bases:

>100_bases
AAAAATTGAAAATAATACTTTTATTACATGACGTTGGTGAGAATAACCACTAACGCCATGCCAGAATTAAACTAAATTTT
GTGCAATACCGTTCATGTTT

Product: GntR family transcriptional regulator

Products: NA

Alternate protein names: L-lactate utilization operon repressor [H]

Number of amino acids: Translated: 237; Mature: 236

Protein sequence:

>237_residues
MSSITKSRSTKKLSQVIVEQLIETIQSGEFKIGEKIPTELELIEQFDVSRSVIREAITELRSLGFVETRHGIGTFVKEQN
IEQNFLLSNASLETINDIVALLELRISLESEAVFLASERRKNKHLEKMKIALNDFEAHISSGENDGTVKADYDFHIAIAE
ASGNQYFVDFLKYLGEKIIPRARVKSIEQSPENREEYLKTVHHDHVNIYNAILDQDGLLARQMMRAHLSKSIKKFKQ

Sequences:

>Translated_237_residues
MSSITKSRSTKKLSQVIVEQLIETIQSGEFKIGEKIPTELELIEQFDVSRSVIREAITELRSLGFVETRHGIGTFVKEQN
IEQNFLLSNASLETINDIVALLELRISLESEAVFLASERRKNKHLEKMKIALNDFEAHISSGENDGTVKADYDFHIAIAE
ASGNQYFVDFLKYLGEKIIPRARVKSIEQSPENREEYLKTVHHDHVNIYNAILDQDGLLARQMMRAHLSKSIKKFKQ
>Mature_236_residues
SSITKSRSTKKLSQVIVEQLIETIQSGEFKIGEKIPTELELIEQFDVSRSVIREAITELRSLGFVETRHGIGTFVKEQNI
EQNFLLSNASLETINDIVALLELRISLESEAVFLASERRKNKHLEKMKIALNDFEAHISSGENDGTVKADYDFHIAIAEA
SGNQYFVDFLKYLGEKIIPRARVKSIEQSPENREEYLKTVHHDHVNIYNAILDQDGLLARQMMRAHLSKSIKKFKQ

Specific function: Negatively regulates the transcription of the lutABC operon, which is required for L-lactate utilization. LutR activity is regulated by lactate, since presence of L-lactate, that probably binds to lutR, leads to derepression of the operon. Also appears to

COG id: COG2186

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786303, Length=226, Percent_Identity=32.3008849557522, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI1790780, Length=235, Percent_Identity=28.0851063829787, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1789353, Length=236, Percent_Identity=27.9661016949153, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1790032, Length=240, Percent_Identity=28.75, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI48994961, Length=165, Percent_Identity=30.9090909090909, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1789621, Length=230, Percent_Identity=27.8260869565217, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI48994955, Length=228, Percent_Identity=27.1929824561404, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 27088; Mature: 26957

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSITKSRSTKKLSQVIVEQLIETIQSGEFKIGEKIPTELELIEQFDVSRSVIREAITEL
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RSLGFVETRHGIGTFVKEQNIEQNFLLSNASLETINDIVALLELRISLESEAVFLASERR
HHCCCHHHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
KNKHLEKMKIALNDFEAHISSGENDGTVKADYDFHIAIAEASGNQYFVDFLKYLGEKIIP
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCEEEEEEEECCCCHHHHHHHHHHHHHCCC
RARVKSIEQSPENREEYLKTVHHDHVNIYNAILDQDGLLARQMMRAHLSKSIKKFKQ
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SSITKSRSTKKLSQVIVEQLIETIQSGEFKIGEKIPTELELIEQFDVSRSVIREAITEL
CCCHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RSLGFVETRHGIGTFVKEQNIEQNFLLSNASLETINDIVALLELRISLESEAVFLASERR
HHCCCHHHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
KNKHLEKMKIALNDFEAHISSGENDGTVKADYDFHIAIAEASGNQYFVDFLKYLGEKIIP
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCEEEEEEEECCCCHHHHHHHHHHHHHCCC
RARVKSIEQSPENREEYLKTVHHDHVNIYNAILDQDGLLARQMMRAHLSKSIKKFKQ
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]