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The map label for this gene is wcaJ [H]

Identifier: 50083388

GI number: 50083388

Start: 94679

End: 96043

Strand: Direct

Name: wcaJ [H]

Synonym: ACIAD0098

Alternate gene names: 50083388

Gene position: 94679-96043 (Clockwise)

Preceding gene: 50083387

Following gene: 50083389

Centisome position: 2.63

GC content: 36.63

Gene sequence:

>1365_bases
ATGGCTTCTAGATTAAATGCATCCTATATCTCAATATTGCAAAGATTTTGTGATGTGGTTGCGATATATTTGGGATTGTA
TGTTGTTTTTAAAATAAACAGTTTGCCGATTAATAATCATACATTGCTCGCATTTTTAAGTATTGTGAGTATTTTCCAAC
TGATTGGTGGTCTTACAGACTTCTATCGTTCATGGCGTGGGATTAGTTTTTTGAGTGAACTAATTTCATGTTTCAAAAAT
ATTACGCTCAGTGTTTGTGTGTTTTATTTGATCAGCTTTTTCTTTGTAAGTATAAAGCTGCTCGAAGTTTTTGAAGTTCT
CTTTTTACTCATTGTTTTTATGAGTATTTTTAGAGGATTAGCGCGGGTTTTATATTCTTTATTTTTTAAAATTATAGATA
ATTCTAAAGGCGTTATGGTGATTGGTGATTCACCCAAAGCCAGTAAGCTTTTTCTTGATCTGGGTATTTCAAAATGGACA
GGTTACAATCCAGTGGGATTGTTTTCATTTAAGATTGATTTTAACCGTTTGAATTTTCATGGGGGCATCCAAGATGCAAT
TACTAAAATTCAGTCAGGAGATATACGTAAAGTTTATATCGTGATTGATCAAGACAATGTGAAGCAGGTTGAGTCATTAC
TTGAGATCTTGGCAGACTCGACCTGTTCAGCAGTGATTATTCCAGATCTATTTTCTTATGATTTTCTCTATTCGCGTGTT
GAAGACGTTAATGGTACGCCAATCATTCCTTTATTTGACTCCCGAATTGACGGGATTAATAATTTTTTAAAGCGGGTTGA
AGATATCGTCATTGGTTCGATGATTTTATTATTGATTTCGCCCCTACTGCTGGTCATTTCGATTGCGATTAAGTTGACTT
CGTCAGGCCCCGTGTTTTTTAAGCAAATTCGTTATGGTCTAAATGGCAAATCCATTCTGGTTTATAAGTTTCGAACCATG
AAAGTGATGGAAAACGGTTCTCAAGTAAAACAAGCTGTAAAGAATGACCCAAGAGTGACCTCTGTTGGACGCTTGTTACG
GAGAACTTCACTGGACGAGTTACCACAATTTTTTAATGTGATTTTAGGTAATATGTCGATTGTGGGTCCACGTCCGCATG
CGGTCGCGCATAATGAGGAATACCGCAAACTGATCCCTGGTTATATGTTACGTCATAAGGTGAAGCCTGGAATTACGGGA
TTGGCTCAAATCCGTGGGTGGCGTGGTGAAACCGATACTTTAGATAAAATGGAAAAACGGATTGAGTGCGATCTTGAATA
CATACGTACGTGGAGCATTTGGCTTGATATTAAGATTATTTTTCTGACCGTATTTCGTGGGTTTATCCATAAAGCAGCGT
ATTAA

Upstream 100 bases:

>100_bases
AATAAACAGAAAGTATAAAACTTACTTTCCTGTAAGTTGTATTTTGATTACGGCATACAGCTTTTTAAATAAAATATAAG
CTTACTTCCAAGGGTCCAAG

Downstream 100 bases:

>100_bases
TAACATTTCTCACTCTGTATCAAGACTTCGAATATTTTAAATAAATTAATTTAAGAGAAGTCCTAATTTTTTAGACTAAG
TGGTTGATAAAATGATCAAA

Product: UDP-glucose lipid carrier transferase/glucose-1-phosphate transferase in colanic acid gene cluster (WcaJ)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 454; Mature: 453

Protein sequence:

>454_residues
MASRLNASYISILQRFCDVVAIYLGLYVVFKINSLPINNHTLLAFLSIVSIFQLIGGLTDFYRSWRGISFLSELISCFKN
ITLSVCVFYLISFFFVSIKLLEVFEVLFLLIVFMSIFRGLARVLYSLFFKIIDNSKGVMVIGDSPKASKLFLDLGISKWT
GYNPVGLFSFKIDFNRLNFHGGIQDAITKIQSGDIRKVYIVIDQDNVKQVESLLEILADSTCSAVIIPDLFSYDFLYSRV
EDVNGTPIIPLFDSRIDGINNFLKRVEDIVIGSMILLLISPLLLVISIAIKLTSSGPVFFKQIRYGLNGKSILVYKFRTM
KVMENGSQVKQAVKNDPRVTSVGRLLRRTSLDELPQFFNVILGNMSIVGPRPHAVAHNEEYRKLIPGYMLRHKVKPGITG
LAQIRGWRGETDTLDKMEKRIECDLEYIRTWSIWLDIKIIFLTVFRGFIHKAAY

Sequences:

>Translated_454_residues
MASRLNASYISILQRFCDVVAIYLGLYVVFKINSLPINNHTLLAFLSIVSIFQLIGGLTDFYRSWRGISFLSELISCFKN
ITLSVCVFYLISFFFVSIKLLEVFEVLFLLIVFMSIFRGLARVLYSLFFKIIDNSKGVMVIGDSPKASKLFLDLGISKWT
GYNPVGLFSFKIDFNRLNFHGGIQDAITKIQSGDIRKVYIVIDQDNVKQVESLLEILADSTCSAVIIPDLFSYDFLYSRV
EDVNGTPIIPLFDSRIDGINNFLKRVEDIVIGSMILLLISPLLLVISIAIKLTSSGPVFFKQIRYGLNGKSILVYKFRTM
KVMENGSQVKQAVKNDPRVTSVGRLLRRTSLDELPQFFNVILGNMSIVGPRPHAVAHNEEYRKLIPGYMLRHKVKPGITG
LAQIRGWRGETDTLDKMEKRIECDLEYIRTWSIWLDIKIIFLTVFRGFIHKAAY
>Mature_453_residues
ASRLNASYISILQRFCDVVAIYLGLYVVFKINSLPINNHTLLAFLSIVSIFQLIGGLTDFYRSWRGISFLSELISCFKNI
TLSVCVFYLISFFFVSIKLLEVFEVLFLLIVFMSIFRGLARVLYSLFFKIIDNSKGVMVIGDSPKASKLFLDLGISKWTG
YNPVGLFSFKIDFNRLNFHGGIQDAITKIQSGDIRKVYIVIDQDNVKQVESLLEILADSTCSAVIIPDLFSYDFLYSRVE
DVNGTPIIPLFDSRIDGINNFLKRVEDIVIGSMILLLISPLLLVISIAIKLTSSGPVFFKQIRYGLNGKSILVYKFRTMK
VMENGSQVKQAVKNDPRVTSVGRLLRRTSLDELPQFFNVILGNMSIVGPRPHAVAHNEEYRKLIPGYMLRHKVKPGITGL
AQIRGWRGETDTLDKMEKRIECDLEYIRTWSIWLDIKIIFLTVFRGFIHKAAY

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=457, Percent_Identity=49.671772428884, Blast_Score=454, Evalue=1e-129,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362
- InterPro:   IPR017475
- InterPro:   IPR017473 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 51691; Mature: 51560

Theoretical pI: Translated: 9.83; Mature: 9.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASRLNASYISILQRFCDVVAIYLGLYVVFKINSLPINNHTLLAFLSIVSIFQLIGGLTD
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHH
FYRSWRGISFLSELISCFKNITLSVCVFYLISFFFVSIKLLEVFEVLFLLIVFMSIFRGL
HHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARVLYSLFFKIIDNSKGVMVIGDSPKASKLFLDLGISKWTGYNPVGLFSFKIDFNRLNFH
HHHHHHHHHHHHCCCCCEEEECCCCCCHHEEEECCCHHCCCCCCCEEEEEEEEECEECCC
GGIQDAITKIQSGDIRKVYIVIDQDNVKQVESLLEILADSTCSAVIIPDLFSYDFLYSRV
CCHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHH
EDVNGTPIIPLFDSRIDGINNFLKRVEDIVIGSMILLLISPLLLVISIAIKLTSSGPVFF
HCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
KQIRYGLNGKSILVYKFRTMKVMENGSQVKQAVKNDPRVTSVGRLLRRTSLDELPQFFNV
HHHHHCCCCCEEEEEEHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHH
ILGNMSIVGPRPHAVAHNEEYRKLIPGYMLRHKVKPGITGLAQIRGWRGETDTLDKMEKR
HHCCCEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCHHHHHHHHH
IECDLEYIRTWSIWLDIKIIFLTVFRGFIHKAAY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ASRLNASYISILQRFCDVVAIYLGLYVVFKINSLPINNHTLLAFLSIVSIFQLIGGLTD
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHH
FYRSWRGISFLSELISCFKNITLSVCVFYLISFFFVSIKLLEVFEVLFLLIVFMSIFRGL
HHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARVLYSLFFKIIDNSKGVMVIGDSPKASKLFLDLGISKWTGYNPVGLFSFKIDFNRLNFH
HHHHHHHHHHHHCCCCCEEEECCCCCCHHEEEECCCHHCCCCCCCEEEEEEEEECEECCC
GGIQDAITKIQSGDIRKVYIVIDQDNVKQVESLLEILADSTCSAVIIPDLFSYDFLYSRV
CCHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHH
EDVNGTPIIPLFDSRIDGINNFLKRVEDIVIGSMILLLISPLLLVISIAIKLTSSGPVFF
HCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
KQIRYGLNGKSILVYKFRTMKVMENGSQVKQAVKNDPRVTSVGRLLRRTSLDELPQFFNV
HHHHHCCCCCEEEEEEHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHH
ILGNMSIVGPRPHAVAHNEEYRKLIPGYMLRHKVKPGITGLAQIRGWRGETDTLDKMEKR
HHCCCEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCHHHHHHHHH
IECDLEYIRTWSIWLDIKIIFLTVFRGFIHKAAY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]