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The map label for this gene is capD [H]

Identifier: 50083387

GI number: 50083387

Start: 92376

End: 94265

Strand: Direct

Name: capD [H]

Synonym: ACIAD0096

Alternate gene names: 50083387

Gene position: 92376-94265 (Clockwise)

Preceding gene: 50083386

Following gene: 50083388

Centisome position: 2.57

GC content: 45.24

Gene sequence:

>1890_bases
ATGTTAAAAACAGGCATGGTCAAAATTTATCTGAATTATTTGTCGGGGCTGGGACGGGTACAAAAAAAAGCCCTGTTACT
GTTCATCGATGCCTGTATGTTGCCACTCCTGATGTGGTTTGCTTTTGCGATCCGACTTTTTAACTACAAAACACCTGTGA
TGGCAGGTTTTTCTTGGTGGCCTGCACTGGTGATTGTTTTAGGCATCAGTTGTCTATGGCTTACTGGCGTGTATCGTGCG
GTGATACGGAGCTTTAACGAAAACTTTATTTTACGTCTTGCCTTTGCCGTAATTCTTAACATGCTGGCGCTATATGTGGT
CGCAAAGCTCCGACTGGCTTTTGTACCGATGAGCATCCCGATTATTTTTGCTTCATTACTATTTGCATGGGTATGGTGTA
GCCGCGCATTTATCCGTATTGTGATCAACAATACCTTGTATAGCCATACCGAAAAAACCCGTGTTGCGATTTATGGTGCG
GGTAATGCGGGTCAGCAAATGGCTTTGGCGCTGGAACGTTCACCCAATCATATTCCTGTGCTTTTTCTGGATGACCACGT
GGGTTTGCAAGGACAAATGGTGCAGGGGTTAAAAGTTTATTCACCTCGACAGCTTAATGAACTCAAACAGCGTTTTCACT
TACATGAAATTTTGCTGGCGATTCCATCGATTGGACGTCAGCGTAAAATGCAGGTGATTCAACAACTGGAATCTCTAGCG
TTAAAAGTGAGGGAAATTCCGAGTGTAGAGCAAATCATTGATGGTCAGTTGCATGTATCCGATATTCGCGAAGTGGATAT
CGAGACCCTGCTTGGACGTGAACCTGTCCCGCCCAATGTTGAGCTTCTGGATAAAAATATCCGAGATAAAGTGGTGCTGG
TGACTGGAGCAGGGGGATCAATTGGTTCTGAGCTATGTCGTCAAATCTTGCAGCGTCAGCCCAAGTTACTCATCTTGTTT
GAGCAATCTGAATTCTCACTCTACAGCATTCATCAGGAATTACAGCAGCGTCATCAAGCCCTGATTCCCATTTTGGGTTC
GGTGTTCAATGAAAAAAAACTGACGCGGATCATGCAGCAGTATCAGGTCCAAACGGTCTATCATGCCGCAGCGTATAAAC
ATGTACCTTTGGTAGAAGCCAATCCGATTGAAGGTCTGCGTAATAACAGTGTTGGAACCGCCAAAAGCTTAAATGCAGCA
GTAACGGCAGGTGTGGAAACTTTTGTTCTGATCTCGACCGATAAAGCCGTACGTCCGACCAATGTCATGGGAGCGTCCAA
GCGTTTGGCCGAATTGTACTGTCAAGCGGTAGCTGCTTCACAGCCCAAGACTAAAATCAGTATTGTACGTTTTGGCAATG
TTTTGGGTTCATCTGGCTCTGTGGTGCCTTTATTTAAAAAGCAGATTGCGGCAGGTGGCCCCATTACAGTGACTCATCCA
GATGTCACCCGTTACTTTATGACCATTCCTGAAGCCGCGTTGCTGGTGATTCAAGCGGGAGCTATGGGTGAGGGCGGCGA
TGTATTTTTATTGGATATGGGAGAGTCAGTTAAAATACGCGATCTGGCATTACAAATGATTGCTTTGAGTGGTCTCAAGC
TGAAAGAAGGCGCCGAGGATGAAGGCGATATCGAGATTCACTATACAGGATTACGTCCAGGTGAAAAGCTTTATGAAGAG
CTCCTGATCGAGCAAAGCAATGTTCAGCCTACAGCACATGAGCGCATTGTCAAATCTTATGAAAAGCAGTTTGCTCTAGA
GCAAATCAAAATCCTGATTGATCTCATCCAGCACAGTTATACCCATCGTGATGATCTAGATGTGATTTTGCAGCAGCTTG
AGCACTATGTCGATGGTTATAAACGCAGTGATCAGGTCGTGGTGAGTTGA

Upstream 100 bases:

>100_bases
ACCAGTCTGATGTTGTTGGTGCATCCCACATTGACGGCTGAAAATATGCAGCAGACCCTCAATGGTATTCAGCATGTTCT
TCAGATGATGGCAGCATAGG

Downstream 100 bases:

>100_bases
GATGCTTCCTAGATATAGCCTTGTACTAGCGGCTTACATTCAAAAGTTACAATCTGATCTAGAATATCGCCCATATATTT
CATAATTCTTAAAAATAAAA

Product: dTDP-glucose-4,6-dehydratase/UDP-glucose 4-epimerase

Products: UDPglucoseal [C]

Alternate protein names: NA

Number of amino acids: Translated: 629; Mature: 629

Protein sequence:

>629_residues
MLKTGMVKIYLNYLSGLGRVQKKALLLFIDACMLPLLMWFAFAIRLFNYKTPVMAGFSWWPALVIVLGISCLWLTGVYRA
VIRSFNENFILRLAFAVILNMLALYVVAKLRLAFVPMSIPIIFASLLFAWVWCSRAFIRIVINNTLYSHTEKTRVAIYGA
GNAGQQMALALERSPNHIPVLFLDDHVGLQGQMVQGLKVYSPRQLNELKQRFHLHEILLAIPSIGRQRKMQVIQQLESLA
LKVREIPSVEQIIDGQLHVSDIREVDIETLLGREPVPPNVELLDKNIRDKVVLVTGAGGSIGSELCRQILQRQPKLLILF
EQSEFSLYSIHQELQQRHQALIPILGSVFNEKKLTRIMQQYQVQTVYHAAAYKHVPLVEANPIEGLRNNSVGTAKSLNAA
VTAGVETFVLISTDKAVRPTNVMGASKRLAELYCQAVAASQPKTKISIVRFGNVLGSSGSVVPLFKKQIAAGGPITVTHP
DVTRYFMTIPEAALLVIQAGAMGEGGDVFLLDMGESVKIRDLALQMIALSGLKLKEGAEDEGDIEIHYTGLRPGEKLYEE
LLIEQSNVQPTAHERIVKSYEKQFALEQIKILIDLIQHSYTHRDDLDVILQQLEHYVDGYKRSDQVVVS

Sequences:

>Translated_629_residues
MLKTGMVKIYLNYLSGLGRVQKKALLLFIDACMLPLLMWFAFAIRLFNYKTPVMAGFSWWPALVIVLGISCLWLTGVYRA
VIRSFNENFILRLAFAVILNMLALYVVAKLRLAFVPMSIPIIFASLLFAWVWCSRAFIRIVINNTLYSHTEKTRVAIYGA
GNAGQQMALALERSPNHIPVLFLDDHVGLQGQMVQGLKVYSPRQLNELKQRFHLHEILLAIPSIGRQRKMQVIQQLESLA
LKVREIPSVEQIIDGQLHVSDIREVDIETLLGREPVPPNVELLDKNIRDKVVLVTGAGGSIGSELCRQILQRQPKLLILF
EQSEFSLYSIHQELQQRHQALIPILGSVFNEKKLTRIMQQYQVQTVYHAAAYKHVPLVEANPIEGLRNNSVGTAKSLNAA
VTAGVETFVLISTDKAVRPTNVMGASKRLAELYCQAVAASQPKTKISIVRFGNVLGSSGSVVPLFKKQIAAGGPITVTHP
DVTRYFMTIPEAALLVIQAGAMGEGGDVFLLDMGESVKIRDLALQMIALSGLKLKEGAEDEGDIEIHYTGLRPGEKLYEE
LLIEQSNVQPTAHERIVKSYEKQFALEQIKILIDLIQHSYTHRDDLDVILQQLEHYVDGYKRSDQVVVS
>Mature_629_residues
MLKTGMVKIYLNYLSGLGRVQKKALLLFIDACMLPLLMWFAFAIRLFNYKTPVMAGFSWWPALVIVLGISCLWLTGVYRA
VIRSFNENFILRLAFAVILNMLALYVVAKLRLAFVPMSIPIIFASLLFAWVWCSRAFIRIVINNTLYSHTEKTRVAIYGA
GNAGQQMALALERSPNHIPVLFLDDHVGLQGQMVQGLKVYSPRQLNELKQRFHLHEILLAIPSIGRQRKMQVIQQLESLA
LKVREIPSVEQIIDGQLHVSDIREVDIETLLGREPVPPNVELLDKNIRDKVVLVTGAGGSIGSELCRQILQRQPKLLILF
EQSEFSLYSIHQELQQRHQALIPILGSVFNEKKLTRIMQQYQVQTVYHAAAYKHVPLVEANPIEGLRNNSVGTAKSLNAA
VTAGVETFVLISTDKAVRPTNVMGASKRLAELYCQAVAASQPKTKISIVRFGNVLGSSGSVVPLFKKQIAAGGPITVTHP
DVTRYFMTIPEAALLVIQAGAMGEGGDVFLLDMGESVKIRDLALQMIALSGLKLKEGAEDEGDIEIHYTGLRPGEKLYEE
LLIEQSNVQPTAHERIVKSYEKQFALEQIKILIDLIQHSYTHRDDLDVILQQLEHYVDGYKRSDQVVVS

Specific function: Required for the biosynthesis of type 1 capsular polysaccharide [H]

COG id: COG1086

COG function: function code MG; Predicted nucleoside-diphosphate sugar epimerases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786974, Length=186, Percent_Identity=28.494623655914, Blast_Score=69, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR003869 [H]

Pfam domain/function: PF02719 Polysacc_synt_2 [H]

EC number: 5.1.3.2 [C]

Molecular weight: Translated: 70406; Mature: 70406

Theoretical pI: Translated: 9.08; Mature: 9.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKTGMVKIYLNYLSGLGRVQKKALLLFIDACMLPLLMWFAFAIRLFNYKTPVMAGFSWW
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCHHH
PALVIVLGISCLWLTGVYRAVIRSFNENFILRLAFAVILNMLALYVVAKLRLAFVPMSIP
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
IIFASLLFAWVWCSRAFIRIVINNTLYSHTEKTRVAIYGAGNAGQQMALALERSPNHIPV
HHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCEEEEEECCCCCCCEEEEEECCCCEEEE
LFLDDHVGLQGQMVQGLKVYSPRQLNELKQRFHLHEILLAIPSIGRQRKMQVIQQLESLA
EEECCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
LKVREIPSVEQIIDGQLHVSDIREVDIETLLGREPVPPNVELLDKNIRDKVVLVTGAGGS
HHHHCCCCHHHHHCCCEEHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCEEEEECCCCC
IGSELCRQILQRQPKLLILFEQSEFSLYSIHQELQQRHQALIPILGSVFNEKKLTRIMQQ
HHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHH
YQVQTVYHAAAYKHVPLVEANPIEGLRNNSVGTAKSLNAAVTAGVETFVLISTDKAVRPT
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHCCHHHCCCCEEEEEEECCCCCCCC
NVMGASKRLAELYCQAVAASQPKTKISIVRFGNVLGSSGSVVPLFKKQIAAGGPITVTHP
CCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECHHHCCCCCEEHHHHHHHCCCCCEEEECC
DVTRYFMTIPEAALLVIQAGAMGEGGDVFLLDMGESVKIRDLALQMIALSGLKLKEGAED
HHHHHHHHCCHHHEEEEEECCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCC
EGDIEIHYTGLRPGEKLYEELLIEQSNVQPTAHERIVKSYEKQFALEQIKILIDLIQHSY
CCCEEEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
THRDDLDVILQQLEHYVDGYKRSDQVVVS
CCCCCHHHHHHHHHHHHCCCCCCCCEEEC
>Mature Secondary Structure
MLKTGMVKIYLNYLSGLGRVQKKALLLFIDACMLPLLMWFAFAIRLFNYKTPVMAGFSWW
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCHHH
PALVIVLGISCLWLTGVYRAVIRSFNENFILRLAFAVILNMLALYVVAKLRLAFVPMSIP
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
IIFASLLFAWVWCSRAFIRIVINNTLYSHTEKTRVAIYGAGNAGQQMALALERSPNHIPV
HHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCEEEEEECCCCCCCEEEEEECCCCEEEE
LFLDDHVGLQGQMVQGLKVYSPRQLNELKQRFHLHEILLAIPSIGRQRKMQVIQQLESLA
EEECCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
LKVREIPSVEQIIDGQLHVSDIREVDIETLLGREPVPPNVELLDKNIRDKVVLVTGAGGS
HHHHCCCCHHHHHCCCEEHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCEEEEECCCCC
IGSELCRQILQRQPKLLILFEQSEFSLYSIHQELQQRHQALIPILGSVFNEKKLTRIMQQ
HHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHH
YQVQTVYHAAAYKHVPLVEANPIEGLRNNSVGTAKSLNAAVTAGVETFVLISTDKAVRPT
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHCCHHHCCCCEEEEEEECCCCCCCC
NVMGASKRLAELYCQAVAASQPKTKISIVRFGNVLGSSGSVVPLFKKQIAAGGPITVTHP
CCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECHHHCCCCCEEHHHHHHHCCCCCEEEECC
DVTRYFMTIPEAALLVIQAGAMGEGGDVFLLDMGESVKIRDLALQMIALSGLKLKEGAED
HHHHHHHHCCHHHEEEEEECCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCC
EGDIEIHYTGLRPGEKLYEELLIEQSNVQPTAHERIVKSYEKQFALEQIKILIDLIQHSY
CCCEEEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
THRDDLDVILQQLEHYVDGYKRSDQVVVS
CCCCCHHHHHHHHHHHHCCCCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDPglucose [C]

Specific reaction: UDPglucose <==> UDPglucoseal [C]

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7961465 [H]