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The map label for this gene is Hgd [H]

Identifier: 50083327

GI number: 50083327

Start: 38279

End: 39151

Strand: Direct

Name: Hgd [H]

Synonym: ACIAD0032

Alternate gene names: 50083327

Gene position: 38279-39151 (Clockwise)

Preceding gene: 50083326

Following gene: 50083339

Centisome position: 1.06

GC content: 47.08

Gene sequence:

>873_bases
ATGCGTATCGGATTTATTGGATTAGGGGCCATGGGCTGGCATATGGCAGGCCACTTGACCCAACTCGATGCCACTGTGAT
GGTCTGGAACCGTACTCAGCATAAAGCCGAAGCACATGCAATGCAGTTTGGTAGTTTGGCGGTGGAGCTAGAACAATTGG
TACAGGCAGATTTAATTTTTTCCTGTTTACCCACCAGTGCCGAGGTGGAAGCTGTGATTGCAGCTTATCCACCACGTTCT
GGCAGTGTCTGGATTGACTGTACCAGTGGTGTTCCTGCATCGGCACAACAACTCAGTCGACACCTCAAACAGCATGGTGT
GGATTTTTTGGATGCACCTGTTTCAGGGCAAACGATTGGCGCTGAAAAGGGCACATTGACGGTGATGATTGGTGGTGAGC
GTACTGCTTTTGAGCGTGCCCAACCTATCATTCAGGCTTTTGCTGGATTGATCGAGCATGTCGGTGACAGTGGAGCAGGT
TTTGCCGTTAAGGCGGTTAATAATATTTTGATGGCTGCTAATCTATGGGCATTGAGTGAGGGCATGTCTGTACTTAAAGC
CCATGCGGTTCATCTAGATGCTGCTTTGGCTTGTATTAATCATTCGAGTGGTAAAAGTACCATGTCTGAAACGATCATGC
CGCAGAAAATTTTAAGCCGTGGCTTTCATAAAACATTTGCACTAAATCTGCTGCAAAAAGATATTGGGATCGCCATCGAC
CTGATAAAAGCTGTAGGCCAACCGGCAGACGTATTACATTGTGTGCAGCAAATGTATAGCGAAATGGATCTTGAGCGCGC
GACACAGGTAGATTTTTCAGCTGCCATTCAAACACTTGAGCAGCGCAATCAATTGCTTCTCGATCTAGCCTAA

Upstream 100 bases:

>100_bases
CAATCGTGATTCTGGCAAGCGCAGTCAGCTTGAACCCAATGCACAGCCTGAGAAAATCATTCAGCCTGTCAGCCCTCAAT
CTTAAGGAGACCGTTGTATC

Downstream 100 bases:

>100_bases
TGAAGACCAAGCCGCATCCTGCGGCTTTTTTTAGGTGGCTAACACAGCTTCAAGCACTTGTTGTTCGAGTTGAGCAAACT
CCAGACTTTTGTGACGTGGA

Product: 3-hydroxyisobutyrate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MRIGFIGLGAMGWHMAGHLTQLDATVMVWNRTQHKAEAHAMQFGSLAVELEQLVQADLIFSCLPTSAEVEAVIAAYPPRS
GSVWIDCTSGVPASAQQLSRHLKQHGVDFLDAPVSGQTIGAEKGTLTVMIGGERTAFERAQPIIQAFAGLIEHVGDSGAG
FAVKAVNNILMAANLWALSEGMSVLKAHAVHLDAALACINHSSGKSTMSETIMPQKILSRGFHKTFALNLLQKDIGIAID
LIKAVGQPADVLHCVQQMYSEMDLERATQVDFSAAIQTLEQRNQLLLDLA

Sequences:

>Translated_290_residues
MRIGFIGLGAMGWHMAGHLTQLDATVMVWNRTQHKAEAHAMQFGSLAVELEQLVQADLIFSCLPTSAEVEAVIAAYPPRS
GSVWIDCTSGVPASAQQLSRHLKQHGVDFLDAPVSGQTIGAEKGTLTVMIGGERTAFERAQPIIQAFAGLIEHVGDSGAG
FAVKAVNNILMAANLWALSEGMSVLKAHAVHLDAALACINHSSGKSTMSETIMPQKILSRGFHKTFALNLLQKDIGIAID
LIKAVGQPADVLHCVQQMYSEMDLERATQVDFSAAIQTLEQRNQLLLDLA
>Mature_290_residues
MRIGFIGLGAMGWHMAGHLTQLDATVMVWNRTQHKAEAHAMQFGSLAVELEQLVQADLIFSCLPTSAEVEAVIAAYPPRS
GSVWIDCTSGVPASAQQLSRHLKQHGVDFLDAPVSGQTIGAEKGTLTVMIGGERTAFERAQPIIQAFAGLIEHVGDSGAG
FAVKAVNNILMAANLWALSEGMSVLKAHAVHLDAALACINHSSGKSTMSETIMPQKILSRGFHKTFALNLLQKDIGIAID
LIKAVGQPADVLHCVQQMYSEMDLERATQVDFSAAIQTLEQRNQLLLDLA

Specific function: Catalyzes the conversion of 2-formylglutarate to (S)-2- hydroxymethylglutarate. Has very low activity with (S)-3- hydroxyisobutyrate [H]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=295, Percent_Identity=29.1525423728814, Blast_Score=107, Evalue=9e-24,
Organism=Homo sapiens, GI40556376, Length=283, Percent_Identity=24.3816254416961, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI145693186, Length=283, Percent_Identity=31.4487632508834, Blast_Score=131, Evalue=6e-32,
Organism=Escherichia coli, GI1786719, Length=290, Percent_Identity=30.6896551724138, Blast_Score=127, Evalue=8e-31,
Organism=Escherichia coli, GI1790315, Length=283, Percent_Identity=27.9151943462898, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI1789092, Length=287, Percent_Identity=26.4808362369338, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1788341, Length=202, Percent_Identity=26.7326732673267, Blast_Score=69, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17557316, Length=298, Percent_Identity=26.510067114094, Blast_Score=95, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24655230, Length=291, Percent_Identity=25.7731958762887, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI19922568, Length=291, Percent_Identity=25.7731958762887, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI28574115, Length=265, Percent_Identity=21.1320754716981, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040
- InterPro:   IPR006183 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: =1.1.1.291 [H]

Molecular weight: Translated: 31118; Mature: 31118

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGFIGLGAMGWHMAGHLTQLDATVMVWNRTQHKAEAHAMQFGSLAVELEQLVQADLIF
CEEEEEECCHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SCLPTSAEVEAVIAAYPPRSGSVWIDCTSGVPASAQQLSRHLKQHGVDFLDAPVSGQTIG
HHCCCCHHHEEEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCEECCCCCCCEEC
AEKGTLTVMIGGERTAFERAQPIIQAFAGLIEHVGDSGAGFAVKAVNNILMAANLWALSE
CCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
GMSVLKAHAVHLDAALACINHSSGKSTMSETIMPQKILSRGFHKTFALNLLQKDIGIAID
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCHHHHHHCCHHHHHHHHHHHHHHHHHHH
LIKAVGQPADVLHCVQQMYSEMDLERATQVDFSAAIQTLEQRNQLLLDLA
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECC
>Mature Secondary Structure
MRIGFIGLGAMGWHMAGHLTQLDATVMVWNRTQHKAEAHAMQFGSLAVELEQLVQADLIF
CEEEEEECCHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SCLPTSAEVEAVIAAYPPRSGSVWIDCTSGVPASAQQLSRHLKQHGVDFLDAPVSGQTIG
HHCCCCHHHEEEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCEECCCCCCCEEC
AEKGTLTVMIGGERTAFERAQPIIQAFAGLIEHVGDSGAGFAVKAVNNILMAANLWALSE
CCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
GMSVLKAHAVHLDAALACINHSSGKSTMSETIMPQKILSRGFHKTFALNLLQKDIGIAID
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCHHHHHHCCHHHHHHHHHHHHHHHHHHH
LIKAVGQPADVLHCVQQMYSEMDLERATQVDFSAAIQTLEQRNQLLLDLA
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA