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The map label for this gene is dsbA [H]

Identifier: 50083339

GI number: 50083339

Start: 49432

End: 50052

Strand: Direct

Name: dsbA [H]

Synonym: ACIAD0045

Alternate gene names: 50083339

Gene position: 49432-50052 (Clockwise)

Preceding gene: 50083327

Following gene: 50083342

Centisome position: 1.37

GC content: 41.22

Gene sequence:

>621_bases
ATGAAAAAATTTGTATTGGGCGGACTTTCAGCAGCGATTATGGCATTTTCGGGCACCACCATGGCTGCCGACTTTGTAGC
AGGTAAAGATTACACGGTGATTGCCAATCCAGGCAAAGTAGATGTACCTGGTAAAATCGAAGTGCGTGAGTTTTTTTGGT
ATGGTTGTCCGCATTGTTATAAGCTTGAACCGTTTATGCAGACTTGGTTAAAGCAGTTACCTAAAGATGTTAATTTTATT
CGTACGCCAGCGGCAATGAATAAAGTATGGGAGCAGGGCGCACGTGCTTACTATGTCTCTGAAGCATTGGGTGTACGTCA
AAAAACGCATTTGCCATTGTTCCATGCGATCCATGCACAAAACCAGCAACTTTTTGATCAGGCGTCTTTGGCAAAATTCT
ATGTGAATTATGGTGTACCTGAACAAAAATTTAATAGCATGTTCAACTCTTTCCCAATCACATCCAAAATTGCAGAATCG
AATAAACTGGCACAGCAATATCAATTGACAGGTGTGCCTGCGATAGTGGTGAATGGTAAATATGTGGTTCAGGGTGAAGA
TGGTAAAGTGACACAAGTGGTTAATTATCTGATCCAGAAAGAACGTACTGCCAAGAAATAA

Upstream 100 bases:

>100_bases
AAATTCAGAAAAAATCTTCACATAACGAAACGAAATTTGAATATTTGTTTTATATTTAACGCATACACTCAGCATAACGG
TTTAGAGGATAACACGGACA

Downstream 100 bases:

>100_bases
CCATGCAGAATTTTTTCTGAATGCATAAAAAAGGTTTCTATGATGTAGAAACCTTTTTTATTGACTGTGATTTAGAGGCG
AGCGTTACGAAAGATCTTTC

Product: thiol:disulfide interchange protein, periplasmic, alkali-inducible

Products: 3-oxo-D 4-steroid; protein with oxidized disulfide bonds [C]

Alternate protein names: NA

Number of amino acids: Translated: 206; Mature: 206

Protein sequence:

>206_residues
MKKFVLGGLSAAIMAFSGTTMAADFVAGKDYTVIANPGKVDVPGKIEVREFFWYGCPHCYKLEPFMQTWLKQLPKDVNFI
RTPAAMNKVWEQGARAYYVSEALGVRQKTHLPLFHAIHAQNQQLFDQASLAKFYVNYGVPEQKFNSMFNSFPITSKIAES
NKLAQQYQLTGVPAIVVNGKYVVQGEDGKVTQVVNYLIQKERTAKK

Sequences:

>Translated_206_residues
MKKFVLGGLSAAIMAFSGTTMAADFVAGKDYTVIANPGKVDVPGKIEVREFFWYGCPHCYKLEPFMQTWLKQLPKDVNFI
RTPAAMNKVWEQGARAYYVSEALGVRQKTHLPLFHAIHAQNQQLFDQASLAKFYVNYGVPEQKFNSMFNSFPITSKIAES
NKLAQQYQLTGVPAIVVNGKYVVQGEDGKVTQVVNYLIQKERTAKK
>Mature_206_residues
MKKFVLGGLSAAIMAFSGTTMAADFVAGKDYTVIANPGKVDVPGKIEVREFFWYGCPHCYKLEPFMQTWLKQLPKDVNFI
RTPAAMNKVWEQGARAYYVSEALGVRQKTHLPLFHAIHAQNQQLFDQASLAKFYVNYGVPEQKFNSMFNSFPITSKIAES
NKLAQQYQLTGVPAIVVNGKYVVQGEDGKVTQVVNYLIQKERTAKK

Specific function: Involved in disulfide-bond formation. Acts by transferring its disulfide bond to other proteins [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 thioredoxin domain [H]

Homologues:

Organism=Escherichia coli, GI1790291, Length=194, Percent_Identity=33.5051546391753, Blast_Score=99, Evalue=3e-22,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 1348 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001853
- InterPro:   IPR023205
- InterPro:   IPR017936
- InterPro:   IPR012336
- InterPro:   IPR017937
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01323 DSBA [H]

EC number: NA

Molecular weight: Translated: 23167; Mature: 23167

Theoretical pI: Translated: 9.91; Mature: 9.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKFVLGGLSAAIMAFSGTTMAADFVAGKDYTVIANPGKVDVPGKIEVREFFWYGCPHCY
CCCEEHHHHHHHHHHHCCCHHHHHHCCCCCEEEEECCCCCCCCCCEEHHHHEECCCCCCE
KLEPFMQTWLKQLPKDVNFIRTPAAMNKVWEQGARAYYVSEALGVRQKTHLPLFHAIHAQ
ECCHHHHHHHHHCCCCCCEEECCHHHHHHHHHCCCEEEHHHHHCCCCCCCCCHHHHHHHC
NQQLFDQASLAKFYVNYGVPEQKFNSMFNSFPITSKIAESNKLAQQYQLTGVPAIVVNGK
CHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEECCE
YVVQGEDGKVTQVVNYLIQKERTAKK
EEEECCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKFVLGGLSAAIMAFSGTTMAADFVAGKDYTVIANPGKVDVPGKIEVREFFWYGCPHCY
CCCEEHHHHHHHHHHHCCCHHHHHHCCCCCEEEEECCCCCCCCCCEEHHHHEECCCCCCE
KLEPFMQTWLKQLPKDVNFIRTPAAMNKVWEQGARAYYVSEALGVRQKTHLPLFHAIHAQ
ECCHHHHHHHHHCCCCCCEEECCHHHHHHHHHCCCEEEHHHHHCCCCCCCCCHHHHHHHC
NQQLFDQASLAKFYVNYGVPEQKFNSMFNSFPITSKIAESNKLAQQYQLTGVPAIVVNGK
CHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEECCE
YVVQGEDGKVTQVVNYLIQKERTAKK
EEEECCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-oxo-D 5-steroid; protein with reduced SH groups [C]

Specific reaction: 3-oxo-D 5-steroid = 3-oxo-D 4-steroid protein with reduced SH groups = protein with oxidized disulfide bonds [C]

General reaction: Isomerases; Intramolecular Oxidoreductases; Transposing S-S Bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10051655 [H]