Click here to switch to the map view.

The map label for this gene is rutB

Identifier: 50083323

GI number: 50083323

Start: 35094

End: 35831

Strand: Direct

Name: rutB

Synonym: ACIAD0028

Alternate gene names: 50083323

Gene position: 35094-35831 (Clockwise)

Preceding gene: 50083322

Following gene: 50083324

Centisome position: 0.98

GC content: 43.5

Gene sequence:

>738_bases
ATGAATACTGTCATTGCAGATTTTACCGAACGTGCAGGCACAGGACGTGTTCTGAAAGCAGAACCTGAGGCCATCCAGCT
AGCGCCCGAGCAAACAGCACTGATCGTGGTTGATATGCAAAATGCCTATACCTCGATTGGAGGATATCTGGACCTCGCGG
GTTTTGATGTTTCTAAAACTAAGCCTGTTGTCGAAAATATTCAAAAAGCAGTTACGGCGGCACATGCTGCTGGCATTCAG
GTAATTTATTTTAAAAATGGTTGGGACAATCAATATGTAGAAGCTGGAGGCGCAGGTTCACCTAATTTTCATAAATCCAA
TGCACTTAAAACCATGCGTAAGCAACCTGAGCTACAAGGCAAATTACTGGCCAAAGGCGGTTGGGATTTTGAACTGATTG
ATGAGTTACAGCCGAAAAGCCAAGATATTGTGATTGAAAAACCACGTTATAGCGGTTTTTTTAATACAGCGCTCGACAGC
ATGTTACGGGCGCGTGGCATTCGTAATCTAGTCTTCGTTGGTATTGCGACCAATGTTTGTGTGGAGTCGACTTTACGGGA
TGGATTTTTTCTCGAATATTTTGGAGTGGCGTTAGACGATGCTTGTTATCAGGCAGGACCAGTCGAAATGCATCGTGCCA
GCATGTACAACATCAAAACCTTTTTTGGCTGGGTGTCGGATACACAAAGTTTTGTTAATACGTTTCAACACAATCAACAG
CTTGCCAAAACTGCTTAA

Upstream 100 bases:

>100_bases
AGGGGGTTGAAGACTTTGGCGAACGCATCCAACCCTTAATGACATCCCGTCAAGATATCGTGGCCGAATATCAACCTGTT
ACCCCATTGGAGAAAAGCGC

Downstream 100 bases:

>100_bases
GGGAGAATCAGAATGCCTAAAGAGATTATTTTACCAGAAGGAACGCCACCACCACTTGCGCCGTATGTGCCAGCGACCAA
GGCAGATAATATCGTTTATG

Product: amidohydrolase

Products: NA

Alternate protein names: Ureidoacrylate amidohydrolase

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MNTVIADFTERAGTGRVLKAEPEAIQLAPEQTALIVVDMQNAYTSIGGYLDLAGFDVSKTKPVVENIQKAVTAAHAAGIQ
VIYFKNGWDNQYVEAGGAGSPNFHKSNALKTMRKQPELQGKLLAKGGWDFELIDELQPKSQDIVIEKPRYSGFFNTALDS
MLRARGIRNLVFVGIATNVCVESTLRDGFFLEYFGVALDDACYQAGPVEMHRASMYNIKTFFGWVSDTQSFVNTFQHNQQ
LAKTA

Sequences:

>Translated_245_residues
MNTVIADFTERAGTGRVLKAEPEAIQLAPEQTALIVVDMQNAYTSIGGYLDLAGFDVSKTKPVVENIQKAVTAAHAAGIQ
VIYFKNGWDNQYVEAGGAGSPNFHKSNALKTMRKQPELQGKLLAKGGWDFELIDELQPKSQDIVIEKPRYSGFFNTALDS
MLRARGIRNLVFVGIATNVCVESTLRDGFFLEYFGVALDDACYQAGPVEMHRASMYNIKTFFGWVSDTQSFVNTFQHNQQ
LAKTA
>Mature_245_residues
MNTVIADFTERAGTGRVLKAEPEAIQLAPEQTALIVVDMQNAYTSIGGYLDLAGFDVSKTKPVVENIQKAVTAAHAAGIQ
VIYFKNGWDNQYVEAGGAGSPNFHKSNALKTMRKQPELQGKLLAKGGWDFELIDELQPKSQDIVIEKPRYSGFFNTALDS
MLRARGIRNLVFVGIATNVCVESTLRDGFFLEYFGVALDDACYQAGPVEMHRASMYNIKTFFGWVSDTQSFVNTFQHNQQ
LAKTA

Specific function: In vivo, quickly hydrolyzes the ureidoacrylate peracid to avoid toxicity, but can also hydrolyzes ureidoacrylate that is formed spontaneously from ureidoacrylate peracid. One of the products of hydrolysis, carbamate, hydrolyzes spontaneously, thereby rele

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismatase family. RutB subfamily

Homologues:

Organism=Escherichia coli, GI87081820, Length=216, Percent_Identity=73.6111111111111, Blast_Score=347, Evalue=3e-97,
Organism=Escherichia coli, GI87081992, Length=211, Percent_Identity=29.8578199052133, Blast_Score=73, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUTB_ACIAD (Q6FFZ6)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_044833.1
- ProteinModelPortal:   Q6FFZ6
- SMR:   Q6FFZ6
- STRING:   Q6FFZ6
- GeneID:   2879058
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD0028
- NMPDR:   fig|62977.3.peg.1093
- eggNOG:   COG1335
- HOGENOM:   HBG752434
- OMA:   MQNAYAS
- PhylomeDB:   Q6FFZ6
- ProtClustDB:   CLSK879924
- BioCyc:   ASP62977:ACIAD0028-MONOMER
- HAMAP:   MF_00830
- InterPro:   IPR019916
- InterPro:   IPR000868
- Gene3D:   G3DSA:3.40.50.850
- TIGRFAMs:   TIGR03614

Pfam domain/function: PF00857 Isochorismatase; SSF52499 Iscrsm_hydrolase

EC number: NA

Molecular weight: Translated: 27044; Mature: 27044

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: NA

Important sites: ACT_SITE 180-180

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTVIADFTERAGTGRVLKAEPEAIQLAPEQTALIVVDMQNAYTSIGGYLDLAGFDVSKT
CCCEECCHHHCCCCCCEEECCCCEEEECCCCEEEEEEECCHHHHHHCCEEEECCCCCCCC
KPVVENIQKAVTAAHAAGIQVIYFKNGWDNQYVEAGGAGSPNFHKSNALKTMRKQPELQG
CHHHHHHHHHHHHHHHCCEEEEEEECCCCCCEEECCCCCCCCCCHHHHHHHHHHCCCCCC
KLLAKGGWDFELIDELQPKSQDIVIEKPRYSGFFNTALDSMLRARGIRNLVFVGIATNVC
EEEECCCCCHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEEHHHHHH
VESTLRDGFFLEYFGVALDDACYQAGPVEMHRASMYNIKTFFGWVSDTQSFVNTFQHNQQ
HHHHHCCCEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAKTA
HHHCC
>Mature Secondary Structure
MNTVIADFTERAGTGRVLKAEPEAIQLAPEQTALIVVDMQNAYTSIGGYLDLAGFDVSKT
CCCEECCHHHCCCCCCEEECCCCEEEECCCCEEEEEEECCHHHHHHCCEEEECCCCCCCC
KPVVENIQKAVTAAHAAGIQVIYFKNGWDNQYVEAGGAGSPNFHKSNALKTMRKQPELQG
CHHHHHHHHHHHHHHHCCEEEEEEECCCCCCEEECCCCCCCCCCHHHHHHHHHHCCCCCC
KLLAKGGWDFELIDELQPKSQDIVIEKPRYSGFFNTALDSMLRARGIRNLVFVGIATNVC
EEEECCCCCHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEEHHHHHH
VESTLRDGFFLEYFGVALDDACYQAGPVEMHRASMYNIKTFFGWVSDTQSFVNTFQHNQQ
HHHHHCCCEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAKTA
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA