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The map label for this gene is rutA

Identifier: 50083322

GI number: 50083322

Start: 33988

End: 35097

Strand: Direct

Name: rutA

Synonym: ACIAD0027

Alternate gene names: 50083322

Gene position: 33988-35097 (Clockwise)

Preceding gene: 50083320

Following gene: 50083323

Centisome position: 0.94

GC content: 44.5

Gene sequence:

>1110_bases
ATGAAAATAGGTGTTTTTTGCCCCATCGGAAACAATGGCTGGCTACTATCCGAAAATGCGCCGCAGTATATGCCAAGCTT
TGAATTGAATAAAAAAATTGTACAACGTGCGGAACATTATGGCTTTGACTTTGCTTTGTCGATGATCAAGCTACGTGGTT
TTGGTGGAAAAACCGAATTTTGGGATCACAATCTGGAAACCTTTACTTTGATGGCTGGACTAGCCGCAGTGACCAGTAAA
ATTAAAATCTATGCCACTGCTGCCACATTGGTCATGCCACCTGCGATTGTTGCGCGTATGGCCTCAACCATTGATTCGAT
CTCTAACGGACGCTTTGGTCTTAATGTGGTGACAGGTTGGCAGGCGCCTGAATATAGTCAAATGGGTATGTGGCCAGGTG
ATGAATACTTTGCCAAACGTTATGAATATCTGTCTGAATATGTACAAATTCTGAGAGAGCTTTGGGCGACAGGACAATCC
GACTTTAAAGGTGAACATTTTCAGATGGAAGATTGTCGAGTTAGCCCTCAACCACAAGCCGAGATGAAAATTATCTGTGC
AGGACAGTCTGATACAGGACTCGAATTTTCTGCACAACATGCTGATTACAATTTCGTGTTTGGTAAGGGCTTAAATACGC
CAACCGCTTACGCAGGCATTAATGACCGTTTGAAACACTTTACCGATCAGACTGGGCGTGATGTTCAAACCTATGTGTTG
TTTATGGTGATTGCCGCAGAAACCGATGCTGAAGCGATGGCCAAGTGGCAAAGCTATAACGATGGTGCCGACGTAGAAGC
CATTAACTGGCTGATGAATCAGGGCGGCAAAGATACTAAATCAGGTGCCGATACCAATATTCGCCAAATGGCATCTAGTG
TTTCACCTGTGAATATCAATATGGGGACTCTCGTGGGTTCCTATGAAAAAGTCGCTGCGATGCTAGACGAAATTGCCGAA
ATTAAAGGCACAGAAGGCATTTTACTGACCTTTGATGATTTTGTGCAGGGGGTTGAAGACTTTGGCGAACGCATCCAACC
CTTAATGACATCCCGTCAAGATATCGTGGCCGAATATCAACCTGTTACCCCATTGGAGAAAAGCGCATGA

Upstream 100 bases:

>100_bases
TAAAAAATATAAGAATAGGCCATTCTTGAAAAAGACAATCATCAGTTTTAGGTGACGCTCATGTAATTCATGAATCGCAT
CTCCCAAGAGGATTACCGAT

Downstream 100 bases:

>100_bases
ATACTGTCATTGCAGATTTTACCGAACGTGCAGGCACAGGACGTGTTCTGAAAGCAGAACCTGAGGCCATCCAGCTAGCG
CCCGAGCAAACAGCACTGAT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 369; Mature: 369

Protein sequence:

>369_residues
MKIGVFCPIGNNGWLLSENAPQYMPSFELNKKIVQRAEHYGFDFALSMIKLRGFGGKTEFWDHNLETFTLMAGLAAVTSK
IKIYATAATLVMPPAIVARMASTIDSISNGRFGLNVVTGWQAPEYSQMGMWPGDEYFAKRYEYLSEYVQILRELWATGQS
DFKGEHFQMEDCRVSPQPQAEMKIICAGQSDTGLEFSAQHADYNFVFGKGLNTPTAYAGINDRLKHFTDQTGRDVQTYVL
FMVIAAETDAEAMAKWQSYNDGADVEAINWLMNQGGKDTKSGADTNIRQMASSVSPVNINMGTLVGSYEKVAAMLDEIAE
IKGTEGILLTFDDFVQGVEDFGERIQPLMTSRQDIVAEYQPVTPLEKSA

Sequences:

>Translated_369_residues
MKIGVFCPIGNNGWLLSENAPQYMPSFELNKKIVQRAEHYGFDFALSMIKLRGFGGKTEFWDHNLETFTLMAGLAAVTSK
IKIYATAATLVMPPAIVARMASTIDSISNGRFGLNVVTGWQAPEYSQMGMWPGDEYFAKRYEYLSEYVQILRELWATGQS
DFKGEHFQMEDCRVSPQPQAEMKIICAGQSDTGLEFSAQHADYNFVFGKGLNTPTAYAGINDRLKHFTDQTGRDVQTYVL
FMVIAAETDAEAMAKWQSYNDGADVEAINWLMNQGGKDTKSGADTNIRQMASSVSPVNINMGTLVGSYEKVAAMLDEIAE
IKGTEGILLTFDDFVQGVEDFGERIQPLMTSRQDIVAEYQPVTPLEKSA
>Mature_369_residues
MKIGVFCPIGNNGWLLSENAPQYMPSFELNKKIVQRAEHYGFDFALSMIKLRGFGGKTEFWDHNLETFTLMAGLAAVTSK
IKIYATAATLVMPPAIVARMASTIDSISNGRFGLNVVTGWQAPEYSQMGMWPGDEYFAKRYEYLSEYVQILRELWATGQS
DFKGEHFQMEDCRVSPQPQAEMKIICAGQSDTGLEFSAQHADYNFVFGKGLNTPTAYAGINDRLKHFTDQTGRDVQTYVL
FMVIAAETDAEAMAKWQSYNDGADVEAINWLMNQGGKDTKSGADTNIRQMASSVSPVNINMGTLVGSYEKVAAMLDEIAE
IKGTEGILLTFDDFVQGVEDFGERIQPLMTSRQDIVAEYQPVTPLEKSA

Specific function: Catalyzes the pyrimidine ring opening between N-3 and C- 4 by an unusual flavin hydroperoxide-catalyzed mechanism to yield ureidoacrylate peracid. It cleaves pyrmidine rings directly by adding oxygen atoms, making a toxic ureidoacrylate peracid product wh

COG id: COG2141

COG function: function code C; Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ntaA/snaA/soxA(dszA) monooxygenase family. RutA subfamily

Homologues:

Organism=Escherichia coli, GI1787247, Length=362, Percent_Identity=72.0994475138122, Blast_Score=568, Evalue=1e-163,
Organism=Escherichia coli, GI1787166, Length=322, Percent_Identity=24.5341614906832, Blast_Score=76, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUTA_ACIAD (Q6FFZ7)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_044832.1
- ProteinModelPortal:   Q6FFZ7
- SMR:   Q6FFZ7
- STRING:   Q6FFZ7
- GeneID:   2879057
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD0027
- NMPDR:   fig|62977.3.peg.1092
- eggNOG:   COG2141
- HOGENOM:   HBG350183
- OMA:   FIPIGNN
- PhylomeDB:   Q6FFZ7
- ProtClustDB:   CLSK879925
- BioCyc:   ASP62977:ACIAD0027-MONOMER
- HAMAP:   MF_01699
- InterPro:   IPR011251
- InterPro:   IPR019914
- InterPro:   IPR016048
- Gene3D:   G3DSA:3.20.20.30
- TIGRFAMs:   TIGR03612

Pfam domain/function: PF00296 Bac_luciferase; SSF51679 Luciferase_like

EC number: NA

Molecular weight: Translated: 40999; Mature: 40999

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: NA

Important sites: BINDING 115-115 BINDING 124-124 BINDING 190-190

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGVFCPIGNNGWLLSENAPQYMPSFELNKKIVQRAEHYGFDFALSMIKLRGFGGKTEF
CCEEEEEEECCCCEEECCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCC
WDHNLETFTLMAGLAAVTSKIKIYATAATLVMPPAIVARMASTIDSISNGRFGLNVVTGW
CCCCCHHHHHHHHHHHHHHCEEEEEEHHHHHCCHHHHHHHHHHHHHHCCCCEEEEEEECC
QAPEYSQMGMWPGDEYFAKRYEYLSEYVQILRELWATGQSDFKGEHFQMEDCRVSPQPQA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEHHCCCCCCCCCC
EMKIICAGQSDTGLEFSAQHADYNFVFGKGLNTPTAYAGINDRLKHFTDQTGRDVQTYVL
CEEEEEECCCCCCCEEEECCCCEEEEEECCCCCCCHHCCHHHHHHHHHCCCCCHHHHHHH
FMVIAAETDAEAMAKWQSYNDGADVEAINWLMNQGGKDTKSGADTNIRQMASSVSPVNIN
HHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCEEE
MGTLVGSYEKVAAMLDEIAEIKGTEGILLTFDDFVQGVEDFGERIQPLMTSRQDIVAEYQ
HHHHHCCHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCHHHHHHHCC
PVTPLEKSA
CCCCCCCCC
>Mature Secondary Structure
MKIGVFCPIGNNGWLLSENAPQYMPSFELNKKIVQRAEHYGFDFALSMIKLRGFGGKTEF
CCEEEEEEECCCCEEECCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCC
WDHNLETFTLMAGLAAVTSKIKIYATAATLVMPPAIVARMASTIDSISNGRFGLNVVTGW
CCCCCHHHHHHHHHHHHHHCEEEEEEHHHHHCCHHHHHHHHHHHHHHCCCCEEEEEEECC
QAPEYSQMGMWPGDEYFAKRYEYLSEYVQILRELWATGQSDFKGEHFQMEDCRVSPQPQA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEHHCCCCCCCCCC
EMKIICAGQSDTGLEFSAQHADYNFVFGKGLNTPTAYAGINDRLKHFTDQTGRDVQTYVL
CEEEEEECCCCCCCEEEECCCCEEEEEECCCCCCCHHCCHHHHHHHHHCCCCCHHHHHHH
FMVIAAETDAEAMAKWQSYNDGADVEAINWLMNQGGKDTKSGADTNIRQMASSVSPVNIN
HHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCEEE
MGTLVGSYEKVAAMLDEIAEIKGTEGILLTFDDFVQGVEDFGERIQPLMTSRQDIVAEYQ
HHHHHCCHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCHHHHHHHCC
PVTPLEKSA
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA