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The map label for this gene is rutR [H]

Identifier: 50083321

GI number: 50083321

Start: 32918

End: 33535

Strand: Reverse

Name: rutR [H]

Synonym: ACIAD0026

Alternate gene names: 50083321

Gene position: 33535-32918 (Counterclockwise)

Preceding gene: 50083328

Following gene: 50083318

Centisome position: 0.93

GC content: 41.26

Gene sequence:

>618_bases
ATGGAACAAAAAAAGAGTACGCAAAAACGCAATCAGCTCCTCAATGCCGCTCTGGATGTATTTTCCACCTATGGCTTTAG
TGGGGCAAGTCTGGACGAAATCGCACAACTCGCGCAGATGCATAAGTCCAATATCTTTTATTATTATGAAAATAAAGAAG
CACTCTATGTAGAAGTGCTGACGACAGTATTACAAAAATGGCTGGCGCCTTTGCAGACGCTTGAAGTCGAGCTTGAGCCA
ACAGAAGCACTGGCACATTATTTAATTCAAAAAATTGAAGTCTCACGGACTCAACCTAAAGCGTCCAAACTGTTCGCACT
GGAGATTATTCAAGGGGCACCACATATTCTGGAAATTTTAAAAGGCCCATTAAAAAAGCTCTTCAAACGCAAAGCTAAAG
TCATCCAGACATGGCAAGAACAAGGTAAAATCTCGGCGGATATTGATCCCGAGCTACTGATTCTAAATATCTGGGCAATT
ACCCAAAACTATGCAGATTTTAGTATCCAAATGGAAATGGTCACAGGCAAAACCCTAAAAAATAAAAGCATGCATCAACG
TGCGATTGAGCACACTGTACACATGATGCTCTATGGTGTTATTCCTCGTTCAGTGTGA

Upstream 100 bases:

>100_bases
CGACTTAGCCCAGATAAAGATAAAGAAAATCGCTCGGGACTGTGCACAAATCGCTCGGATTCCCTTACAATGAGCTGACC
TTTCCCAATTTGTTATCTCA

Downstream 100 bases:

>100_bases
CAAGATCAGATCAGTTGATTAAGGATGTGCCATCGTTTTTACGATGGCATTTTTATGCTTCAAACTTATATTTTAAGTTG
GTGCTGATGTCTTAAGAAAA

Product: TetR/AcrR family transcriptional regulator

Products: NA

Alternate protein names: Rut operon repressor [H]

Number of amino acids: Translated: 205; Mature: 205

Protein sequence:

>205_residues
MEQKKSTQKRNQLLNAALDVFSTYGFSGASLDEIAQLAQMHKSNIFYYYENKEALYVEVLTTVLQKWLAPLQTLEVELEP
TEALAHYLIQKIEVSRTQPKASKLFALEIIQGAPHILEILKGPLKKLFKRKAKVIQTWQEQGKISADIDPELLILNIWAI
TQNYADFSIQMEMVTGKTLKNKSMHQRAIEHTVHMMLYGVIPRSV

Sequences:

>Translated_205_residues
MEQKKSTQKRNQLLNAALDVFSTYGFSGASLDEIAQLAQMHKSNIFYYYENKEALYVEVLTTVLQKWLAPLQTLEVELEP
TEALAHYLIQKIEVSRTQPKASKLFALEIIQGAPHILEILKGPLKKLFKRKAKVIQTWQEQGKISADIDPELLILNIWAI
TQNYADFSIQMEMVTGKTLKNKSMHQRAIEHTVHMMLYGVIPRSV
>Mature_205_residues
MEQKKSTQKRNQLLNAALDVFSTYGFSGASLDEIAQLAQMHKSNIFYYYENKEALYVEVLTTVLQKWLAPLQTLEVELEP
TEALAHYLIQKIEVSRTQPKASKLFALEIIQGAPHILEILKGPLKKLFKRKAKVIQTWQEQGKISADIDPELLILNIWAI
TQNYADFSIQMEMVTGKTLKNKSMHQRAIEHTVHMMLYGVIPRSV

Specific function: Master transcription regulator which represses the degradation of pyrimidines (rutABCDEFG) and purines (gcl operon) for maintenance of metabolic balance between pyrimidines and purines. It also regulates the synthesis of pyrimidine nucleotides and arginin

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH tetR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787249, Length=197, Percent_Identity=43.6548223350254, Blast_Score=181, Evalue=3e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR015893
- InterPro:   IPR011075
- InterPro:   IPR019915
- InterPro:   IPR001647
- InterPro:   IPR013573 [H]

Pfam domain/function: PF08362 TetR_C_3; PF00440 TetR_N [H]

EC number: NA

Molecular weight: Translated: 23515; Mature: 23515

Theoretical pI: Translated: 9.42; Mature: 9.42

Prosite motif: PS50977 HTH_TETR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQKKSTQKRNQLLNAALDVFSTYGFSGASLDEIAQLAQMHKSNIFYYYENKEALYVEVL
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCEEEEEECCCCHHHHHH
TTVLQKWLAPLQTLEVELEPTEALAHYLIQKIEVSRTQPKASKLFALEIIQGAPHILEIL
HHHHHHHHHHHHHHEEEECHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHH
KGPLKKLFKRKAKVIQTWQEQGKISADIDPELLILNIWAITQNYADFSIQMEMVTGKTLK
HHHHHHHHHHHHHHHHHHHHCCCEECCCCCCEEEEEHHHHHCCCCCEEEEEEEECCCHHH
NKSMHQRAIEHTVHMMLYGVIPRSV
HHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MEQKKSTQKRNQLLNAALDVFSTYGFSGASLDEIAQLAQMHKSNIFYYYENKEALYVEVL
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCEEEEEECCCCHHHHHH
TTVLQKWLAPLQTLEVELEPTEALAHYLIQKIEVSRTQPKASKLFALEIIQGAPHILEIL
HHHHHHHHHHHHHHEEEECHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHH
KGPLKKLFKRKAKVIQTWQEQGKISADIDPELLILNIWAITQNYADFSIQMEMVTGKTLK
HHHHHHHHHHHHHHHHHHHHCCCEECCCCCCEEEEEHHHHHCCCCCEEEEEEEECCCHHH
NKSMHQRAIEHTVHMMLYGVIPRSV
HHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]