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The map label for this gene is ribF [H]

Identifier: 50083318

GI number: 50083318

Start: 29666

End: 30667

Strand: Reverse

Name: ribF [H]

Synonym: ACIAD0023

Alternate gene names: 50083318

Gene position: 30667-29666 (Counterclockwise)

Preceding gene: 50083321

Following gene: 50083317

Centisome position: 0.85

GC content: 44.01

Gene sequence:

>1002_bases
ATGAAACTGCTCCGTCTTAATGCGTTATCGCCAGATTTTGAGTTAGCTGCCACTGCGGTCACGATTGGCAATTTTGATGG
CGTCCATCTTGGACATCAGGCCATGATTAGTCAGCTTAAAAATATTGCAGATGCGAACAGCCTGAAAACGTTAGTGATGA
TTTTTGAGCCTCAGCCGCTTGAATACTTCAAGGGTTATGAAGCACCGCCACGCATCAGTTCACTCAGAGAAAAAGTCGAA
TACTTGACTGAATTGGGCGTCGATTATATTGCGATTGCAAAATTTGATCATTATTTCCGTGGACTCAGTGCCCAAGAATT
TGCAGACCTACTCAAATTTAAGCTGAATGCCAAACAGCTTGTACTGGGTGACGATTTTCATTTTGGTAAAAATCGTCAGG
GAAATAGTGAATTTTTACGGGATTATGGCTTTAAAATCACCAACCTTGACACCATAGAGTTCAAAGGAGAACGTGTCAGT
TCCACCCGTATTCGTCAGGTTCTGGCAGAGGGTGATCTGGCATTGGCAGCCAAGTTACTTGGACGTCCATATAGTATGAT
TGGTCGTGTGCAATATGGTGACCAAATTGGTCGTACACTCAATTTTCCAACCATCAATGTCCGTTTAGGCCGGCATAAAC
CGTGTCTGAGTGGTATTTATGGTGTAGATGTCGTCTGTGAAACAACGTCTTTACGCAAGAAGATCATGCATGATCCGAAT
GCACCTAAAGGTATTGCAGGCTACGACCCAGACAGCTTATATGGTGCGGGTCATGTTGGAACTCGTCCTGCCATCAAACA
GGCGCATCCCGAATGGCGATTGGAAGTACATTTTCCAGAAGTTTCTGCTAATCTGTATGGCCTATTAATGCGGGTGACGT
TTTTACACTATTTACATGGTGAGCGAAACTATCCTTCGCTAGACGCGCTGAAAGCTGGGATTGACCAAGATGTTGAGGAT
TTGCTCGAATTTCGTAAGCAAAATACCCAACGCCCTTTTTAA

Upstream 100 bases:

>100_bases
AATGTTAAGTTTTCTTTTGCATAAAAACACAACAACAAGACGAATCCATTAGATTATGGCAATATATAAGCCTTTCAAAA
ATTAATCTTCACTGAAGTCC

Downstream 100 bases:

>100_bases
TTTAAAATTTTTGATGTTAAAAACACGTCAGCTTTAACGCTGATCGAACTGGAAGACAACTTGCATGAGCGATAAGCAAA
CTCCTGAAAATGCAGTGGAC

Product: bifunctional riboflavin kinase/FMN adenylyltransferase

Products: NA

Alternate protein names: Riboflavin kinase; Flavokinase; FMN adenylyltransferase; FAD pyrophosphorylase; FAD synthase [H]

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MKLLRLNALSPDFELAATAVTIGNFDGVHLGHQAMISQLKNIADANSLKTLVMIFEPQPLEYFKGYEAPPRISSLREKVE
YLTELGVDYIAIAKFDHYFRGLSAQEFADLLKFKLNAKQLVLGDDFHFGKNRQGNSEFLRDYGFKITNLDTIEFKGERVS
STRIRQVLAEGDLALAAKLLGRPYSMIGRVQYGDQIGRTLNFPTINVRLGRHKPCLSGIYGVDVVCETTSLRKKIMHDPN
APKGIAGYDPDSLYGAGHVGTRPAIKQAHPEWRLEVHFPEVSANLYGLLMRVTFLHYLHGERNYPSLDALKAGIDQDVED
LLEFRKQNTQRPF

Sequences:

>Translated_333_residues
MKLLRLNALSPDFELAATAVTIGNFDGVHLGHQAMISQLKNIADANSLKTLVMIFEPQPLEYFKGYEAPPRISSLREKVE
YLTELGVDYIAIAKFDHYFRGLSAQEFADLLKFKLNAKQLVLGDDFHFGKNRQGNSEFLRDYGFKITNLDTIEFKGERVS
STRIRQVLAEGDLALAAKLLGRPYSMIGRVQYGDQIGRTLNFPTINVRLGRHKPCLSGIYGVDVVCETTSLRKKIMHDPN
APKGIAGYDPDSLYGAGHVGTRPAIKQAHPEWRLEVHFPEVSANLYGLLMRVTFLHYLHGERNYPSLDALKAGIDQDVED
LLEFRKQNTQRPF
>Mature_333_residues
MKLLRLNALSPDFELAATAVTIGNFDGVHLGHQAMISQLKNIADANSLKTLVMIFEPQPLEYFKGYEAPPRISSLREKVE
YLTELGVDYIAIAKFDHYFRGLSAQEFADLLKFKLNAKQLVLGDDFHFGKNRQGNSEFLRDYGFKITNLDTIEFKGERVS
STRIRQVLAEGDLALAAKLLGRPYSMIGRVQYGDQIGRTLNFPTINVRLGRHKPCLSGIYGVDVVCETTSLRKKIMHDPN
APKGIAGYDPDSLYGAGHVGTRPAIKQAHPEWRLEVHFPEVSANLYGLLMRVTFLHYLHGERNYPSLDALKAGIDQDVED
LLEFRKQNTQRPF

Specific function: Unknown

COG id: COG0196

COG function: function code H; FAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribF family [H]

Homologues:

Organism=Escherichia coli, GI1786208, Length=330, Percent_Identity=41.2121212121212, Blast_Score=227, Evalue=8e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015864
- InterPro:   IPR015865
- InterPro:   IPR002606
- InterPro:   IPR014729 [H]

Pfam domain/function: PF06574 FAD_syn; PF01687 Flavokinase [H]

EC number: =2.7.1.26; =2.7.7.2 [H]

Molecular weight: Translated: 37556; Mature: 37556

Theoretical pI: Translated: 8.57; Mature: 8.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLRLNALSPDFELAATAVTIGNFDGVHLGHQAMISQLKNIADANSLKTLVMIFEPQPL
CCEEEECCCCCCHHHHEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCH
EYFKGYEAPPRISSLREKVEYLTELGVDYIAIAKFDHYFRGLSAQEFADLLKFKLNAKQL
HHHCCCCCCHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHCCCCHHHHHHHHHHHCCCCEE
VLGDDFHFGKNRQGNSEFLRDYGFKITNLDTIEFKGERVSSTRIRQVLAEGDLALAAKLL
EECCCCCCCCCCCCCHHHHHHHCCEEECCEEEEECCCCHHHHHHHHHHHCCCHHHHHHHH
GRPYSMIGRVQYGDQIGRTLNFPTINVRLGRHKPCLSGIYGVDVVCETTSLRKKIMHDPN
CCCHHHHHCCHHHHHHCCEECCCEEEEEECCCCCHHHCCCCCEEEECCHHHHHHHHCCCC
APKGIAGYDPDSLYGAGHVGTRPAIKQAHPEWRLEVHFPEVSANLYGLLMRVTFLHYLHG
CCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCC
ERNYPSLDALKAGIDQDVEDLLEFRKQNTQRPF
CCCCCHHHHHHHCCCCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKLLRLNALSPDFELAATAVTIGNFDGVHLGHQAMISQLKNIADANSLKTLVMIFEPQPL
CCEEEECCCCCCHHHHEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCH
EYFKGYEAPPRISSLREKVEYLTELGVDYIAIAKFDHYFRGLSAQEFADLLKFKLNAKQL
HHHCCCCCCHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHCCCCHHHHHHHHHHHCCCCEE
VLGDDFHFGKNRQGNSEFLRDYGFKITNLDTIEFKGERVSSTRIRQVLAEGDLALAAKLL
EECCCCCCCCCCCCCHHHHHHHCCEEECCEEEEECCCCHHHHHHHHHHHCCCHHHHHHHH
GRPYSMIGRVQYGDQIGRTLNFPTINVRLGRHKPCLSGIYGVDVVCETTSLRKKIMHDPN
CCCHHHHHCCHHHHHHCCEECCCEEEEEECCCCCHHHCCCCCEEEECCHHHHHHHHCCCC
APKGIAGYDPDSLYGAGHVGTRPAIKQAHPEWRLEVHFPEVSANLYGLLMRVTFLHYLHG
CCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCC
ERNYPSLDALKAGIDQDVEDLLEFRKQNTQRPF
CCCCCHHHHHHHCCCCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]