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The map label for this gene is rutD

Identifier: 50083320

GI number: 50083320

Start: 32045

End: 32845

Strand: Direct

Name: rutD

Synonym: ACIAD0025

Alternate gene names: 50083320

Gene position: 32045-32845 (Clockwise)

Preceding gene: 50083319

Following gene: 50083322

Centisome position: 0.89

GC content: 43.45

Gene sequence:

>801_bases
ATGAACTATCAACTTTTTAAGCACTCCGATGAAAATGCATCTTATGTGGTGTTTTCATCGGGTCTGGGTGGGCATGGCAG
TTTTTGGCAGGCACAACTGGATGTATTTCGGCAGTATTTTCATGTATTGATTTATGATCAGGAAGGGTGTCACGCGTCGA
GTGAGTTGTTAGCTGATGGCTATTCATTTGAGCATCTGGCCTTACAAGTCAAGCAGTTATTACAGCAACTCAACATCGTA
CGCTTTCATTTTATTGGACATGCATTAGGCGGATTTATTGGGATAGAACTGGCGCATCGTTATGCATCTGAAACGTGTCA
ACTGTTAAGCCTGACCTTGATTAATGCCTGGCAGCAGCTCGATCCACACACCTTAAGATGTTTTACCACCCGTATCGCGT
TATTACAGCATGCAGGCACAGCAGCATATTTACATGCTCAGGCTTTGTTTTTGTATCCACCCTTATGGATTTCTGAACAT
ACAGCTTTACTTGAGCAGCAAGAGGCCAAAATGCAATCCGATTTTCCGCCACATGCCAATGTCTTAAAGCGGTTAAATGC
CCTCATGCAGTATCAGGTAAACACTGCACGTATAGACACGTTAAAACAATTGCCTGTGTGCCTGATCGCTAATCAGGACG
ATATGTTGGTGCCATACGTACAATCTTTGAATTTATGGAAAAAATTACCAGATGCGCAGCTCAAGCTACTGCCTTATGGG
GGACATGCATCTACAGTCACTGAGGCTCGGCAGGTCAATCAACTGATGCTTGATTTTCTTAAGACATCAGCACCAACTTA
A

Upstream 100 bases:

>100_bases
GCTTGCAGCAATTACAGCAACAACAAAAGGCGTGCGCACAATCTGAAACGGCATAAATCTTGCTGATAATTTAGATCATG
ATTTTACCAAATGGTTAAAA

Downstream 100 bases:

>100_bases
AATATAAGTTTGAAGCATAAAAATGCCATCGTAAAAACGATGGCACATCCTTAATCAACTGATCTGATCTTGTCACACTG
AACGAGGAATAACACCATAG

Product: alpha/beta family hydrolase

Products: NA

Alternate protein names: Aminohydrolase

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MNYQLFKHSDENASYVVFSSGLGGHGSFWQAQLDVFRQYFHVLIYDQEGCHASSELLADGYSFEHLALQVKQLLQQLNIV
RFHFIGHALGGFIGIELAHRYASETCQLLSLTLINAWQQLDPHTLRCFTTRIALLQHAGTAAYLHAQALFLYPPLWISEH
TALLEQQEAKMQSDFPPHANVLKRLNALMQYQVNTARIDTLKQLPVCLIANQDDMLVPYVQSLNLWKKLPDAQLKLLPYG
GHASTVTEARQVNQLMLDFLKTSAPT

Sequences:

>Translated_266_residues
MNYQLFKHSDENASYVVFSSGLGGHGSFWQAQLDVFRQYFHVLIYDQEGCHASSELLADGYSFEHLALQVKQLLQQLNIV
RFHFIGHALGGFIGIELAHRYASETCQLLSLTLINAWQQLDPHTLRCFTTRIALLQHAGTAAYLHAQALFLYPPLWISEH
TALLEQQEAKMQSDFPPHANVLKRLNALMQYQVNTARIDTLKQLPVCLIANQDDMLVPYVQSLNLWKKLPDAQLKLLPYG
GHASTVTEARQVNQLMLDFLKTSAPT
>Mature_266_residues
MNYQLFKHSDENASYVVFSSGLGGHGSFWQAQLDVFRQYFHVLIYDQEGCHASSELLADGYSFEHLALQVKQLLQQLNIV
RFHFIGHALGGFIGIELAHRYASETCQLLSLTLINAWQQLDPHTLRCFTTRIALLQHAGTAAYLHAQALFLYPPLWISEH
TALLEQQEAKMQSDFPPHANVLKRLNALMQYQVNTARIDTLKQLPVCLIANQDDMLVPYVQSLNLWKKLPDAQLKLLPYG
GHASTVTEARQVNQLMLDFLKTSAPT

Specific function: May increase the rate of spontaneous hydrolysis of aminoacrylate to malonic semialdehyde. Required to remove a toxic intermediate produce in the pyrimidine nitrogen degradation

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Hydrolase RutD family

Homologues:

Organism=Escherichia coli, GI1787244, Length=244, Percent_Identity=38.9344262295082, Blast_Score=170, Evalue=1e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUTD_ACIAD (Q6FFZ9)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_044830.1
- ProteinModelPortal:   Q6FFZ9
- STRING:   Q6FFZ9
- GeneID:   2880163
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD0025
- NMPDR:   fig|62977.3.peg.1090
- eggNOG:   COG0596
- HOGENOM:   HBG748003
- OMA:   CIVQEMA
- PhylomeDB:   Q6FFZ9
- BioCyc:   ASP62977:ACIAD0025-MONOMER
- HAMAP:   MF_00832
- InterPro:   IPR000073
- InterPro:   IPR019913
- TIGRFAMs:   TIGR03611

Pfam domain/function: PF00561 Abhydrolase_1

EC number: NA

Molecular weight: Translated: 30161; Mature: 30161

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYQLFKHSDENASYVVFSSGLGGHGSFWQAQLDVFRQYFHVLIYDQEGCHASSELLADG
CCCCEEEECCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHCC
YSFEHLALQVKQLLQQLNIVRFHFIGHALGGFIGIELAHRYASETCQLLSLTLINAWQQL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DPHTLRCFTTRIALLQHAGTAAYLHAQALFLYPPLWISEHTALLEQQEAKMQSDFPPHAN
CCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHH
VLKRLNALMQYQVNTARIDTLKQLPVCLIANQDDMLVPYVQSLNLWKKLPDAQLKLLPYG
HHHHHHHHHHHCCCHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEECC
GHASTVTEARQVNQLMLDFLKTSAPT
CCHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNYQLFKHSDENASYVVFSSGLGGHGSFWQAQLDVFRQYFHVLIYDQEGCHASSELLADG
CCCCEEEECCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHCC
YSFEHLALQVKQLLQQLNIVRFHFIGHALGGFIGIELAHRYASETCQLLSLTLINAWQQL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DPHTLRCFTTRIALLQHAGTAAYLHAQALFLYPPLWISEHTALLEQQEAKMQSDFPPHAN
CCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHH
VLKRLNALMQYQVNTARIDTLKQLPVCLIANQDDMLVPYVQSLNLWKKLPDAQLKLLPYG
HHHHHHHHHHHCCCHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEECC
GHASTVTEARQVNQLMLDFLKTSAPT
CCHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA