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The map label for this gene is gyrB [H]

Identifier: 50083301

GI number: 50083301

Start: 4127

End: 6595

Strand: Direct

Name: gyrB [H]

Synonym: ACIAD0004

Alternate gene names: 50083301

Gene position: 4127-6595 (Clockwise)

Preceding gene: 50083300

Following gene: 50083303

Centisome position: 0.11

GC content: 44.67

Gene sequence:

>2469_bases
ATGAGTTCAGAATCGCAATCTGCCTCTCCAACAGAACAAACAATTGAAAAGGCGTATGATTCCTCTAGTATCAAGGTATT
ACGTGGGCTGGATGCTGTTCGTAAACGTCCAGGCATGTATATTGGTGATACTGACGATGGGACTGGCTTGCACCATATGG
TGTTTGAAGTCGTCGATAATGCGATTGATGAAGCTTTAGCCGGACACTGTGATGAGATTATTGTCACCATTCATGAAGAT
GAATCGGTGAGCGTCTCTGATAATGGTCGTGGTATCCCAACTGATATCCATCCTGAAGAGGGTGTTTCTGCCGCAGAAGT
GATTCTAACCATTCTGCATGCTGGCGGTAAGTTTGATGATAATAGTTATAAGGTTTCTGGCGGTCTGCATGGTGTAGGTG
TGTCAGTGGTCAATGCCTTGTCCAGCAAGCTACAGTTAACCATTCATCGTGCTGGTCAGATCCATGAACAGGAATATCAG
CACGGCGATCCTCAGTATCCGCTAAAAGTGGTCGGTGAGACATCTACCACGGGTACTACTGTACGTTTCTGGCCAAGTGG
GGATACTTTTAGCCAGACTATTTTTAATGTTGATATTCTGGCACGCCGTTTACGTGAGCTGTCTTTTCTAAATGCAGGCG
TTAAAATAGTCCTGCGTGATGAACGTGTGAACTTTGAGCATATTTATGCCTATGAAGGCGGCCTGTCAGAGTTTGTAAAA
TACATCAATGAAGGCAAAAATCACCTCAATGACATTTTCCATTTTACCGCTGATTCTGACAATGGCATTGCCGTTGAAGT
GGCGTTGCAATGGAATGACAGCTATCAGGAAAATGTACGCTGCTTTACCAACAATATTCCACAAAAAGATGGTGGAACAC
ACCTTGCGGGCTTCCGTGCAGCTTTGACACGTGGTTTAAATAGTTATCTTGAGAATGAAAATATTCTTAAGAAAGAAAAA
GTTAACGTGAGTGGTGATGATGCACGTGAAGGTTTGACCGCTATTATTTCGGTCAAGGTGCCTGATCCAAAATTCTCGTC
TCAAACCAAAGAAAAATTGGTTTCAAGTGAAGTCAAACCTGCGGTTGAACAGGCGATGAACAAGGAGTTTTCGGCTTATC
TGCTGGAAAATCCGCAAGCAGCAAAATCGATTGCTGGAAAAATTATTGATGCCGCACGTGCGCGTGATGCCGCACGTAAA
GCACGTGAAATGACGCGTCGTAAGAGTGCACTCGATATTGCGGGCTTACCGGGTAAACTTGCAGATTGTCAGGAAAAAGA
TCCAGCGTTGTCTGAGTTGTATCTGGTCGAGGGTGACTCTGCGGGTGGTAGTGCTAAGCAAGGTCGTAACCGTAAGATGC
AGGCGATCTTGCCGCTGAAAGGTAAAATCCTGAACGTGGAACGTGCGCGTTTTGACAAGATGATTTCCAGTCAGGAAGTG
GGAACATTGATCACCGCGCTGGGCTGTGGTATTGGTCGTGAAGAATATAATCCCGATAAGCTTCGTTACCACAAAATCAT
CATTATGACCGATGCGGATGTCGATGGTTCACACATTCGAACCTTACTATTGACTTTCTTCTTCCGTCAAATGCCAGAAC
TGGTTGAGCGTGGTCATATTTATATCGCCCAGCCACCATTGTACAAGTTGAAAAAAGGTAAGCAGGAACAGTACATCAAA
GATAATGATGCACTTGAAACCTTCCTGATTTCAAATGCCATTGATGATCTGGCATTGCATATCAGTGCAGAAGCACCTGC
CATTACAGGTGAAGCACTGGCTAAAGTCATTGATGACTACAAGATTTCGCAAAAAAGTTTGCAGCGTTTGACCCAGCGTT
ATCCTGCAACTTTGCTTGATGGCTTGCTCGAAGTTGAGCCATTTAAAGCCGATCTTAGCCATGATCGTCCCTATGTAGAG
CAGTGGGCCGAGCAACTGGGTCAAGTGATTGCAAAATTACAGCCAAGCTTACGACCTGAGGTGAGTTTAGAAAGCTTTGA
ACGTGAATCACCAACAGGGGAAACTTCGATTCATTGTTGGCCGCGTATTACGGTCTATGTGCATAATTTACCACACCATT
ACCTATTGGATGCTGGTTTGCTCAACTCTGCTGAATATGCACGATTACTCAAAAACTCCAAGAGCTGGTTTAAATTGCTT
GAAGAAGGTGCTTACTTGCAAAAAGGTGAACGTCGTATTCAGGTGAGCAATTTCCATCAGGTTTGGCAGCAAATCTTGCA
GGACTCACGTCGTGGCATGATGATTCAGCGCTATAAAGGTTTAGGTGAGATGAACGCCGAGCAGCTTTGGGAAACTACGA
TGGATCCTGATAACCGTCATATGCTGCAAGTCACCATTGATGATGCGATTGAAGCTGATCGTATGTTTAGTTGCCTCATG
GGCGATGACGTCGAGCCACGTCGTGCATTTATTGAGGAAAATGCGTTGAATGCAGATATTGATGCTTAA

Upstream 100 bases:

>100_bases
TCTTATCAATTATACAATGTTGATCATGGTCAAGTTCATGCTGTTTGACGTTGATTTTTTACCCATCTTTGGATAGACCT
ATTTTTGCTAGGGAGAAACC

Downstream 100 bases:

>100_bases
TATCTGATTGATTAAAACACCATAAAAAAATCAGGCACACAGTGCCTGATTTTTTTGACCATTTAATTTCCATAGGGGTC
ATGTGACTTCCTGTCTAGGG

Product: DNA gyrase, subunit B (type II topoisomerase)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 822; Mature: 821

Protein sequence:

>822_residues
MSSESQSASPTEQTIEKAYDSSSIKVLRGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNAIDEALAGHCDEIIVTIHED
ESVSVSDNGRGIPTDIHPEEGVSAAEVILTILHAGGKFDDNSYKVSGGLHGVGVSVVNALSSKLQLTIHRAGQIHEQEYQ
HGDPQYPLKVVGETSTTGTTVRFWPSGDTFSQTIFNVDILARRLRELSFLNAGVKIVLRDERVNFEHIYAYEGGLSEFVK
YINEGKNHLNDIFHFTADSDNGIAVEVALQWNDSYQENVRCFTNNIPQKDGGTHLAGFRAALTRGLNSYLENENILKKEK
VNVSGDDAREGLTAIISVKVPDPKFSSQTKEKLVSSEVKPAVEQAMNKEFSAYLLENPQAAKSIAGKIIDAARARDAARK
AREMTRRKSALDIAGLPGKLADCQEKDPALSELYLVEGDSAGGSAKQGRNRKMQAILPLKGKILNVERARFDKMISSQEV
GTLITALGCGIGREEYNPDKLRYHKIIIMTDADVDGSHIRTLLLTFFFRQMPELVERGHIYIAQPPLYKLKKGKQEQYIK
DNDALETFLISNAIDDLALHISAEAPAITGEALAKVIDDYKISQKSLQRLTQRYPATLLDGLLEVEPFKADLSHDRPYVE
QWAEQLGQVIAKLQPSLRPEVSLESFERESPTGETSIHCWPRITVYVHNLPHHYLLDAGLLNSAEYARLLKNSKSWFKLL
EEGAYLQKGERRIQVSNFHQVWQQILQDSRRGMMIQRYKGLGEMNAEQLWETTMDPDNRHMLQVTIDDAIEADRMFSCLM
GDDVEPRRAFIEENALNADIDA

Sequences:

>Translated_822_residues
MSSESQSASPTEQTIEKAYDSSSIKVLRGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNAIDEALAGHCDEIIVTIHED
ESVSVSDNGRGIPTDIHPEEGVSAAEVILTILHAGGKFDDNSYKVSGGLHGVGVSVVNALSSKLQLTIHRAGQIHEQEYQ
HGDPQYPLKVVGETSTTGTTVRFWPSGDTFSQTIFNVDILARRLRELSFLNAGVKIVLRDERVNFEHIYAYEGGLSEFVK
YINEGKNHLNDIFHFTADSDNGIAVEVALQWNDSYQENVRCFTNNIPQKDGGTHLAGFRAALTRGLNSYLENENILKKEK
VNVSGDDAREGLTAIISVKVPDPKFSSQTKEKLVSSEVKPAVEQAMNKEFSAYLLENPQAAKSIAGKIIDAARARDAARK
AREMTRRKSALDIAGLPGKLADCQEKDPALSELYLVEGDSAGGSAKQGRNRKMQAILPLKGKILNVERARFDKMISSQEV
GTLITALGCGIGREEYNPDKLRYHKIIIMTDADVDGSHIRTLLLTFFFRQMPELVERGHIYIAQPPLYKLKKGKQEQYIK
DNDALETFLISNAIDDLALHISAEAPAITGEALAKVIDDYKISQKSLQRLTQRYPATLLDGLLEVEPFKADLSHDRPYVE
QWAEQLGQVIAKLQPSLRPEVSLESFERESPTGETSIHCWPRITVYVHNLPHHYLLDAGLLNSAEYARLLKNSKSWFKLL
EEGAYLQKGERRIQVSNFHQVWQQILQDSRRGMMIQRYKGLGEMNAEQLWETTMDPDNRHMLQVTIDDAIEADRMFSCLM
GDDVEPRRAFIEENALNADIDA
>Mature_821_residues
SSESQSASPTEQTIEKAYDSSSIKVLRGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNAIDEALAGHCDEIIVTIHEDE
SVSVSDNGRGIPTDIHPEEGVSAAEVILTILHAGGKFDDNSYKVSGGLHGVGVSVVNALSSKLQLTIHRAGQIHEQEYQH
GDPQYPLKVVGETSTTGTTVRFWPSGDTFSQTIFNVDILARRLRELSFLNAGVKIVLRDERVNFEHIYAYEGGLSEFVKY
INEGKNHLNDIFHFTADSDNGIAVEVALQWNDSYQENVRCFTNNIPQKDGGTHLAGFRAALTRGLNSYLENENILKKEKV
NVSGDDAREGLTAIISVKVPDPKFSSQTKEKLVSSEVKPAVEQAMNKEFSAYLLENPQAAKSIAGKIIDAARARDAARKA
REMTRRKSALDIAGLPGKLADCQEKDPALSELYLVEGDSAGGSAKQGRNRKMQAILPLKGKILNVERARFDKMISSQEVG
TLITALGCGIGREEYNPDKLRYHKIIIMTDADVDGSHIRTLLLTFFFRQMPELVERGHIYIAQPPLYKLKKGKQEQYIKD
NDALETFLISNAIDDLALHISAEAPAITGEALAKVIDDYKISQKSLQRLTQRYPATLLDGLLEVEPFKADLSHDRPYVEQ
WAEQLGQVIAKLQPSLRPEVSLESFERESPTGETSIHCWPRITVYVHNLPHHYLLDAGLLNSAEYARLLKNSKSWFKLLE
EGAYLQKGERRIQVSNFHQVWQQILQDSRRGMMIQRYKGLGEMNAEQLWETTMDPDNRHMLQVTIDDAIEADRMFSCLMG
DDVEPRRAFIEENALNADIDA

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=552, Percent_Identity=25.9057971014493, Blast_Score=132, Evalue=1e-30,
Organism=Homo sapiens, GI19913406, Length=561, Percent_Identity=26.0249554367201, Blast_Score=129, Evalue=9e-30,
Organism=Escherichia coli, GI48994957, Length=808, Percent_Identity=67.0792079207921, Blast_Score=1064, Evalue=0.0,
Organism=Escherichia coli, GI1789408, Length=550, Percent_Identity=42.5454545454545, Blast_Score=415, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI17535065, Length=533, Percent_Identity=25.703564727955, Blast_Score=144, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI212645845, Length=505, Percent_Identity=24.950495049505, Blast_Score=132, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI212645657, Length=160, Percent_Identity=33.75, Blast_Score=74, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6324241, Length=592, Percent_Identity=25.6756756756757, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI17136538, Length=544, Percent_Identity=26.4705882352941, Blast_Score=145, Evalue=9e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 91788; Mature: 91657

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSESQSASPTEQTIEKAYDSSSIKVLRGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDN
CCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHH
AIDEALAGHCDEIIVTIHEDESVSVSDNGRGIPTDIHPEEGVSAAEVILTILHAGGKFDD
HHHHHHHCCCCEEEEEEECCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCC
NSYKVSGGLHGVGVSVVNALSSKLQLTIHRAGQIHEQEYQHGDPQYPLKVVGETSTTGTT
CCEEECCCCCHHHHHHHHHHHHHHEEEEECCCCHHHHHHHCCCCCCCEEEEECCCCCCCE
VRFWPSGDTFSQTIFNVDILARRLRELSFLNAGVKIVLRDERVNFEHIYAYEGGLSEFVK
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEEEECCCHHHHHH
YINEGKNHLNDIFHFTADSDNGIAVEVALQWNDSYQENVRCFTNNIPQKDGGTHLAGFRA
HHHHHHHHHHHHHEEECCCCCCEEEEEEEEECCCHHHHHHHHHCCCCCCCCCCHHHHHHH
ALTRGLNSYLENENILKKEKVNVSGDDAREGLTAIISVKVPDPKFSSQTKEKLVSSEVKP
HHHHHHHHHHCCCCCHHHHCCCCCCCCHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHH
AVEQAMNKEFSAYLLENPQAAKSIAGKIIDAARARDAARKAREMTRRKSALDIAGLPGKL
HHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
ADCQEKDPALSELYLVEGDSAGGSAKQGRNRKMQAILPLKGKILNVERARFDKMISSQEV
CCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEECCCCEEECHHHHHHHHHHHHHHH
GTLITALGCGIGREEYNPDKLRYHKIIIMTDADVDGSHIRTLLLTFFFRQMPELVERGHI
HHHHHHHHCCCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCE
YIAQPPLYKLKKGKQEQYIKDNDALETFLISNAIDDLALHISAEAPAITGEALAKVIDDY
EEECCCHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHH
KISQKSLQRLTQRYPATLLDGLLEVEPFKADLSHDRPYVEQWAEQLGQVIAKLQPSLRPE
CCCHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
VSLESFERESPTGETSIHCWPRITVYVHNLPHHYLLDAGLLNSAEYARLLKNSKSWFKLL
CCHHHHHCCCCCCCCEEEEEEEEEEEEECCCCHHHHHCHHCCHHHHHHHHHCCHHHHHHH
EEGAYLQKGERRIQVSNFHQVWQQILQDSRRGMMIQRYKGLGEMNAEQLWETTMDPDNRH
HCCCHHHCCCCEEEHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCHHHHHHHCCCCCCCE
MLQVTIDDAIEADRMFSCLMGDDVEPRRAFIEENALNADIDA
EEEEEECHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SSESQSASPTEQTIEKAYDSSSIKVLRGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDN
CCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHH
AIDEALAGHCDEIIVTIHEDESVSVSDNGRGIPTDIHPEEGVSAAEVILTILHAGGKFDD
HHHHHHHCCCCEEEEEEECCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCC
NSYKVSGGLHGVGVSVVNALSSKLQLTIHRAGQIHEQEYQHGDPQYPLKVVGETSTTGTT
CCEEECCCCCHHHHHHHHHHHHHHEEEEECCCCHHHHHHHCCCCCCCEEEEECCCCCCCE
VRFWPSGDTFSQTIFNVDILARRLRELSFLNAGVKIVLRDERVNFEHIYAYEGGLSEFVK
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEEEECCCHHHHHH
YINEGKNHLNDIFHFTADSDNGIAVEVALQWNDSYQENVRCFTNNIPQKDGGTHLAGFRA
HHHHHHHHHHHHHEEECCCCCCEEEEEEEEECCCHHHHHHHHHCCCCCCCCCCHHHHHHH
ALTRGLNSYLENENILKKEKVNVSGDDAREGLTAIISVKVPDPKFSSQTKEKLVSSEVKP
HHHHHHHHHHCCCCCHHHHCCCCCCCCHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHH
AVEQAMNKEFSAYLLENPQAAKSIAGKIIDAARARDAARKAREMTRRKSALDIAGLPGKL
HHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
ADCQEKDPALSELYLVEGDSAGGSAKQGRNRKMQAILPLKGKILNVERARFDKMISSQEV
CCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEECCCCEEECHHHHHHHHHHHHHHH
GTLITALGCGIGREEYNPDKLRYHKIIIMTDADVDGSHIRTLLLTFFFRQMPELVERGHI
HHHHHHHHCCCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCE
YIAQPPLYKLKKGKQEQYIKDNDALETFLISNAIDDLALHISAEAPAITGEALAKVIDDY
EEECCCHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHH
KISQKSLQRLTQRYPATLLDGLLEVEPFKADLSHDRPYVEQWAEQLGQVIAKLQPSLRPE
CCCHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
VSLESFERESPTGETSIHCWPRITVYVHNLPHHYLLDAGLLNSAEYARLLKNSKSWFKLL
CCHHHHHCCCCCCCCEEEEEEEEEEEEECCCCHHHHHCHHCCHHHHHHHHHCCHHHHHHH
EEGAYLQKGERRIQVSNFHQVWQQILQDSRRGMMIQRYKGLGEMNAEQLWETTMDPDNRH
HCCCHHHCCCCEEEHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCHHHHHHHCCCCCCCE
MLQVTIDDAIEADRMFSCLMGDDVEPRRAFIEENALNADIDA
EEEEEECHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12620739; 9464408 [H]