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The map label for this gene is recF

Identifier: 50083300

GI number: 50083300

Start: 2998

End: 4074

Strand: Direct

Name: recF

Synonym: ACIAD0003

Alternate gene names: 50083300

Gene position: 2998-4074 (Clockwise)

Preceding gene: 50083299

Following gene: 50083301

Centisome position: 0.08

GC content: 42.06

Gene sequence:

>1077_bases
ATGCAAATTACGCGTTTAAACATTGAACGTGTGCGCAATTTAAAAGCGGTTGCACTCTCTGGATTGCAGCCGTTTAATAT
TTTTTATGGCGCCAACGGTTCAGGTAAAACTTCTATTCTAGAAGCGGTCCATCTTCTGGCGACTGGACGTTCGTTTAGAA
CCCATATGCCCAAGCATTACATTCAGCAGAATGCACAGGATGCCATCATTTTTGCCCAGTCTTTGTCTGAAAAAATTGGC
ATGCAAAAGCTGTTATCAGGGGAGCAATTAATTAAGGTCAATGGCGATACTGTCGCCACTCAGGGACAATTGGCCAAACT
GTTACCCTTACAACATTTAGACCCACAGAGCACAGATATTATTGATCATGGTGCAAAACCGCGGCGACAGTTGCTCGATT
GGTTAATGTTCCACGTGGAACCTGAGTTTTATTTTGCGTGGCAATATTATTCACGGGCGCTCAAGCAGCGTAATATGTTG
CTCAAAACCAAACGTCAGCTTAGTTTGGCAGAGCTGGAACCGTGGAACAAAATGCTAAGTGAATACGGGGAAATGCTACA
CTCACAGCGACTGGTGACCGTAGAGCGCTGGAAAGATTTTTTTCAACAAGATTTGGCGCAGCTATTGCCTGATCTTCAGA
TTGAACTGGAATACAGCCCAGGTTTTCATAGCGAAGTAGGATTATGGCAAGACCTTTTGAATTATCACAATAAAGATGTT
GAACGACGTTATACAGAGTACGGTCCACACCGCGCAGACTTGCGCCTAAAAACTGCATTGGGTGATGCCGATGATGTATT
GTCACGTGGGCAAAAGAAATTACTGATGATGGCATTAAAACTGTCACAGATTGCAATGTTACATGCGAGTAATAAGGAAA
CTGTGGTATTATTAGATGATCTGACAGCAGAATTAGATAGTAACGCGCAACGACGTTTAATTGAACGATTGAGCCAATTG
GGTAGTCAAGTTTTTATTACCACTTTAGATCATCAGGCTGTAACTCAACATTTAGATGGTCTGTCTATTTCTTATCAATT
ATACAATGTTGATCATGGTCAAGTTCATGCTGTTTGA

Upstream 100 bases:

>100_bases
TATGACTGAGGCCAATCAGTCGGTTTTGGTTCAAGATGCGGCACATCCTGATCAGACCTATGTGGTCATGCCAATGCGTG
TGTGATGTATTGACTAAATT

Downstream 100 bases:

>100_bases
CGTTGATTTTTTACCCATCTTTGGATAGACCTATTTTTGCTAGGGAGAAACCATGAGTTCAGAATCGCAATCTGCCTCTC
CAACAGAACAAACAATTGAA

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MQITRLNIERVRNLKAVALSGLQPFNIFYGANGSGKTSILEAVHLLATGRSFRTHMPKHYIQQNAQDAIIFAQSLSEKIG
MQKLLSGEQLIKVNGDTVATQGQLAKLLPLQHLDPQSTDIIDHGAKPRRQLLDWLMFHVEPEFYFAWQYYSRALKQRNML
LKTKRQLSLAELEPWNKMLSEYGEMLHSQRLVTVERWKDFFQQDLAQLLPDLQIELEYSPGFHSEVGLWQDLLNYHNKDV
ERRYTEYGPHRADLRLKTALGDADDVLSRGQKKLLMMALKLSQIAMLHASNKETVVLLDDLTAELDSNAQRRLIERLSQL
GSQVFITTLDHQAVTQHLDGLSISYQLYNVDHGQVHAV

Sequences:

>Translated_358_residues
MQITRLNIERVRNLKAVALSGLQPFNIFYGANGSGKTSILEAVHLLATGRSFRTHMPKHYIQQNAQDAIIFAQSLSEKIG
MQKLLSGEQLIKVNGDTVATQGQLAKLLPLQHLDPQSTDIIDHGAKPRRQLLDWLMFHVEPEFYFAWQYYSRALKQRNML
LKTKRQLSLAELEPWNKMLSEYGEMLHSQRLVTVERWKDFFQQDLAQLLPDLQIELEYSPGFHSEVGLWQDLLNYHNKDV
ERRYTEYGPHRADLRLKTALGDADDVLSRGQKKLLMMALKLSQIAMLHASNKETVVLLDDLTAELDSNAQRRLIERLSQL
GSQVFITTLDHQAVTQHLDGLSISYQLYNVDHGQVHAV
>Mature_358_residues
MQITRLNIERVRNLKAVALSGLQPFNIFYGANGSGKTSILEAVHLLATGRSFRTHMPKHYIQQNAQDAIIFAQSLSEKIG
MQKLLSGEQLIKVNGDTVATQGQLAKLLPLQHLDPQSTDIIDHGAKPRRQLLDWLMFHVEPEFYFAWQYYSRALKQRNML
LKTKRQLSLAELEPWNKMLSEYGEMLHSQRLVTVERWKDFFQQDLAQLLPDLQIELEYSPGFHSEVGLWQDLLNYHNKDV
ERRYTEYGPHRADLRLKTALGDADDVLSRGQKKLLMMALKLSQIAMLHASNKETVVLLDDLTAELDSNAQRRLIERLSQL
GSQVFITTLDHQAVTQHLDGLSISYQLYNVDHGQVHAV

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family

Homologues:

Organism=Escherichia coli, GI1790135, Length=361, Percent_Identity=33.5180055401662, Blast_Score=196, Evalue=3e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RECF_ACIAD (Q6FG19)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_044810.1
- ProteinModelPortal:   Q6FG19
- STRING:   Q6FG19
- GeneID:   2879480
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD0003
- NMPDR:   fig|62977.3.peg.1008
- eggNOG:   COG1195
- HOGENOM:   HBG645124
- OMA:   GQQRTLV
- PhylomeDB:   Q6FG19
- ProtClustDB:   CLSK706806
- BioCyc:   ASP62977:ACIAD0003-MONOMER
- GO:   GO:0005694
- GO:   GO:0005737
- HAMAP:   MF_00365
- InterPro:   IPR001238
- InterPro:   IPR003395
- TIGRFAMs:   TIGR00611

Pfam domain/function: PF02463 SMC_N

EC number: NA

Molecular weight: Translated: 41078; Mature: 41078

Theoretical pI: Translated: 7.59; Mature: 7.59

Prosite motif: PS00617 RECF_1; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQITRLNIERVRNLKAVALSGLQPFNIFYGANGSGKTSILEAVHLLATGRSFRTHMPKHY
CCEEEECHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCHHHHCCCHHH
IQQNAQDAIIFAQSLSEKIGMQKLLSGEQLIKVNGDTVATQGQLAKLLPLQHLDPQSTDI
HHCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEECCCHHHHHCCHHCCCCCCCCH
IDHGAKPRRQLLDWLMFHVEPEFYFAWQYYSRALKQRNMLLKTKRQLSLAELEPWNKMLS
HHCCCCHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
EYGEMLHSQRLVTVERWKDFFQQDLAQLLPDLQIELEYSPGFHSEVGLWQDLLNYHNKDV
HHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHCCCHH
ERRYTEYGPHRADLRLKTALGDADDVLSRGQKKLLMMALKLSQIAMLHASNKETVVLLDD
HHHHHHCCCCCCCEEEEHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEC
LTAELDSNAQRRLIERLSQLGSQVFITTLDHQAVTQHLDGLSISYQLYNVDHGQVHAV
HHHHHCCCHHHHHHHHHHHCCCEEEEEECCHHHHHHHCCCCEEEEEEEECCCCEEECC
>Mature Secondary Structure
MQITRLNIERVRNLKAVALSGLQPFNIFYGANGSGKTSILEAVHLLATGRSFRTHMPKHY
CCEEEECHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCHHHHCCCHHH
IQQNAQDAIIFAQSLSEKIGMQKLLSGEQLIKVNGDTVATQGQLAKLLPLQHLDPQSTDI
HHCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEECCCHHHHHCCHHCCCCCCCCH
IDHGAKPRRQLLDWLMFHVEPEFYFAWQYYSRALKQRNMLLKTKRQLSLAELEPWNKMLS
HHCCCCHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
EYGEMLHSQRLVTVERWKDFFQQDLAQLLPDLQIELEYSPGFHSEVGLWQDLLNYHNKDV
HHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHCCCHH
ERRYTEYGPHRADLRLKTALGDADDVLSRGQKKLLMMALKLSQIAMLHASNKETVVLLDD
HHHHHHCCCCCCCEEEEHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEC
LTAELDSNAQRRLIERLSQLGSQVFITTLDHQAVTQHLDGLSISYQLYNVDHGQVHAV
HHHHHCCCHHHHHHHHHHHCCCEEEEEECCHHHHHHHCCCCEEEEEEEECCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA