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The map label for this gene is dnaN [H]

Identifier: 50083299

GI number: 50083299

Start: 1834

End: 2982

Strand: Direct

Name: dnaN [H]

Synonym: ACIAD0002

Alternate gene names: 50083299

Gene position: 1834-2982 (Clockwise)

Preceding gene: 50083298

Following gene: 50083300

Centisome position: 0.05

GC content: 44.13

Gene sequence:

>1149_bases
GTGCGACTAAAAATAGCCAAAGAAAGTTTACTCAATGTTCTCTCCCATGTGGTGGGTGCAGTAGAGCGTCGCCATACGTT
GAATATTCTTTCAAATGTTAAAATTCAGGCTAATGCGCAGGCTTTGACCATTACTGGATCTGATCTTGAAGTTGAACTGG
TCGCAAGTACGACATTAGCAGAAGGGGCATGCATTGAAGCTGGAGAGACCACAGTACCTGCACGAAAACTGGTCGATATC
TGTAAATCTCTTCCTTCTGCTGCCTTGATTGATCTACAAATTACCGAAGATCAGCGCTGTATCCTGAAGTCTGGAAATAG
CCGTTTTGTGCTGGGCACTTTACCAGCAGAAGACTATCCGCTATTGACCACTGAGAGCAGTCAAGGTACTCAGGTTCAAG
TGACTCAGCGCGAGTTGAAGCGTTTGTTTGAAAAAACATCTTTTGCTATGGCAGTTCAGGATGTGCGTTTTTATTTGACG
GGCACATTACTAGAAATTGATCAAAATCAGTTACGTGCAGTCACGACTGATGGGCATCGTTTGGCACTGTGTGAAGTTCA
GGCATCATCCACCGCCATGCAGGCAGTACAAGCAATTGTGCCACGTAAAGCTGTGGGTGAATTACAGCGTTTGTTAAGTA
TCGAAGATGATCAGCTAAGTTTGCTGATTGGTCGTGAACTATTGAATGTGACCATTAACATTGCCAATCGTGATAAGGAA
CAACATCCAATTACCGTGCGCTTTACCACCAAATTAATTGATGGAAAGTTTCCTGATTATCGCCGTGTCATTCCACGTGG
TGGCGATAAACACGTACAAATTGCGCATGACGTGTTCAAGCAGTCACTACAGCGTGTCGCGATTTTGAGTAATGAGAAAT
TACGCGGCGTATTCCTGAATTTTAATCCTGATGTGTTACAGCTTCGTGCCAATAACCCAGAGCAGGATGAGGCAATTGAA
GATATTGCAATTCAATATCAGGATGCATCGCTTGAAATGTCCTTTAATGCGCAGTATTTGCTAGATGTCTTAAGTGTACT
GGATGGTGATGATGTCAGCATGAGTATGACTGAGGCCAATCAGTCGGTTTTGGTTCAAGATGCGGCACATCCTGATCAGA
CCTATGTGGTCATGCCAATGCGTGTGTGA

Upstream 100 bases:

>100_bases
GCTGATGCTCCATCGATCATATTGATAAAATTTTCGTCCTGTCTTTTACATTAAATTAGGTTAGAATACTTCAAGTTTTT
TTGAGTTTAAGGGTTAAAAC

Downstream 100 bases:

>100_bases
TGTATTGACTAAATTATGCAAATTACGCGTTTAAACATTGAACGTGTGCGCAATTTAAAAGCGGTTGCACTCTCTGGATT
GCAGCCGTTTAATATTTTTT

Product: DNA polymerase III beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 382; Mature: 382

Protein sequence:

>382_residues
MRLKIAKESLLNVLSHVVGAVERRHTLNILSNVKIQANAQALTITGSDLEVELVASTTLAEGACIEAGETTVPARKLVDI
CKSLPSAALIDLQITEDQRCILKSGNSRFVLGTLPAEDYPLLTTESSQGTQVQVTQRELKRLFEKTSFAMAVQDVRFYLT
GTLLEIDQNQLRAVTTDGHRLALCEVQASSTAMQAVQAIVPRKAVGELQRLLSIEDDQLSLLIGRELLNVTINIANRDKE
QHPITVRFTTKLIDGKFPDYRRVIPRGGDKHVQIAHDVFKQSLQRVAILSNEKLRGVFLNFNPDVLQLRANNPEQDEAIE
DIAIQYQDASLEMSFNAQYLLDVLSVLDGDDVSMSMTEANQSVLVQDAAHPDQTYVVMPMRV

Sequences:

>Translated_382_residues
MRLKIAKESLLNVLSHVVGAVERRHTLNILSNVKIQANAQALTITGSDLEVELVASTTLAEGACIEAGETTVPARKLVDI
CKSLPSAALIDLQITEDQRCILKSGNSRFVLGTLPAEDYPLLTTESSQGTQVQVTQRELKRLFEKTSFAMAVQDVRFYLT
GTLLEIDQNQLRAVTTDGHRLALCEVQASSTAMQAVQAIVPRKAVGELQRLLSIEDDQLSLLIGRELLNVTINIANRDKE
QHPITVRFTTKLIDGKFPDYRRVIPRGGDKHVQIAHDVFKQSLQRVAILSNEKLRGVFLNFNPDVLQLRANNPEQDEAIE
DIAIQYQDASLEMSFNAQYLLDVLSVLDGDDVSMSMTEANQSVLVQDAAHPDQTYVVMPMRV
>Mature_382_residues
MRLKIAKESLLNVLSHVVGAVERRHTLNILSNVKIQANAQALTITGSDLEVELVASTTLAEGACIEAGETTVPARKLVDI
CKSLPSAALIDLQITEDQRCILKSGNSRFVLGTLPAEDYPLLTTESSQGTQVQVTQRELKRLFEKTSFAMAVQDVRFYLT
GTLLEIDQNQLRAVTTDGHRLALCEVQASSTAMQAVQAIVPRKAVGELQRLLSIEDDQLSLLIGRELLNVTINIANRDKE
QHPITVRFTTKLIDGKFPDYRRVIPRGGDKHVQIAHDVFKQSLQRVAILSNEKLRGVFLNFNPDVLQLRANNPEQDEAIE
DIAIQYQDASLEMSFNAQYLLDVLSVLDGDDVSMSMTEANQSVLVQDAAHPDQTYVVMPMRV

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=382, Percent_Identity=44.2408376963351, Blast_Score=310, Evalue=8e-86,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 42341; Mature: 42341

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLKIAKESLLNVLSHVVGAVERRHTLNILSNVKIQANAQALTITGSDLEVELVASTTLA
CCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCEEEEEECCCEEEEEEEECCCC
EGACIEAGETTVPARKLVDICKSLPSAALIDLQITEDQRCILKSGNSRFVLGTLPAEDYP
CCCEEECCCCCCCHHHHHHHHHCCCCCEEEEEEECCCCEEEEECCCCEEEEEECCCCCCC
LLTTESSQGTQVQVTQRELKRLFEKTSFAMAVQDVRFYLTGTLLEIDQNQLRAVTTDGHR
EEEECCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEECCCCEEEEECCCCE
LALCEVQASSTAMQAVQAIVPRKAVGELQRLLSIEDDQLSLLIGRELLNVTINIANRDKE
EEEEEECCHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCEEHEECCEEEEEEEEEECCCCC
QHPITVRFTTKLIDGKFPDYRRVIPRGGDKHVQIAHDVFKQSLQRVAILSNEKLRGVFLN
CCCEEEEEEEEECCCCCCCHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCEEEEEEE
FNPDVLQLRANNPEQDEAIEDIAIQYQDASLEMSFNAQYLLDVLSVLDGDDVSMSMTEAN
CCCCEEEEECCCCCHHHHHHHHEEEEECCEEEEECCHHHHHHHHHHHCCCCCEEEEECCC
QSVLVQDAAHPDQTYVVMPMRV
CEEEEEECCCCCCEEEEEEECC
>Mature Secondary Structure
MRLKIAKESLLNVLSHVVGAVERRHTLNILSNVKIQANAQALTITGSDLEVELVASTTLA
CCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCEEEEEECCCEEEEEEEECCCC
EGACIEAGETTVPARKLVDICKSLPSAALIDLQITEDQRCILKSGNSRFVLGTLPAEDYP
CCCEEECCCCCCCHHHHHHHHHCCCCCEEEEEEECCCCEEEEECCCCEEEEEECCCCCCC
LLTTESSQGTQVQVTQRELKRLFEKTSFAMAVQDVRFYLTGTLLEIDQNQLRAVTTDGHR
EEEECCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEECCCCEEEEECCCCE
LALCEVQASSTAMQAVQAIVPRKAVGELQRLLSIEDDQLSLLIGRELLNVTINIANRDKE
EEEEEECCHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCEEHEECCEEEEEEEEEECCCCC
QHPITVRFTTKLIDGKFPDYRRVIPRGGDKHVQIAHDVFKQSLQRVAILSNEKLRGVFLN
CCCEEEEEEEEECCCCCCCHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCEEEEEEE
FNPDVLQLRANNPEQDEAIEDIAIQYQDASLEMSFNAQYLLDVLSVLDGDDVSMSMTEAN
CCCCEEEEECCCCCHHHHHHHHEEEEECCEEEEECCHHHHHHHHHHHCCCCCEEEEECCC
QSVLVQDAAHPDQTYVVMPMRV
CEEEEEECCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]