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The map label for this gene is braZ [H]

Identifier: 161349964

GI number: 161349964

Start: 1978715

End: 1980016

Strand: Direct

Name: braZ [H]

Synonym: ACIAD1988

Alternate gene names: 161349964

Gene position: 1978715-1980016 (Clockwise)

Preceding gene: 50085119

Following gene: 50085126

Centisome position: 54.99

GC content: 39.32

Gene sequence:

>1302_bases
ATGACTAGTCTTCGTACTTCAGATATTATTGCATTGGGTTTTATGACCTTTGCTCTATTTATTGGGGCTGGTAACATTAT
TTTCCCACCCATTGTTGCTCAGCAAGCTGGCGAGCACGTATGGTTAGCTGCTGCTGGTTTCTTGATTACAGCCGTTGGAC
TACCTGTAATTACGATTATGGCTTTATCGCGTATGGAAGGTTCCATCGCAATGATCAGCTCTCCTTTAGGTCGTTTTTTT
AGCCTGCTACTCACCATCGTTTGTTACTTATCTGTTGGGCCATTATTTGCAACACCAAGGACTGCAACCGTTTCATATGA
AATTGGTTTTTCTTCTTACTTTGGAACATCAAGTGGCAGTCTCTTTGTTTATAGCTTGATTTATTTTTCCATTGTGACAC
TGGTATCACTTTATCCGAATAAATTACTCGATACTGTTGGCCATGTACTTGCACCCCTTAAGATTATTGCATTGGCAATC
TTAGGTATTGCAGCAGTCCTCATCCCATCTAATTATATTCCACCAGCAATTAACAACTATGTAGAAAACCCTGTATCAGA
AGGTTTTGTAAATGGTTACCTCACGATGGATACATTAGGTGCATTGGTTTTTGGTATTGTGATTATTCAGGCAATTTACT
CACGTGGTGTACGTGATCCAAAACTGGTAACCAAATATGCAATCATTGCCAGTATCATCTCGGGTATTGGCCTTACTTTG
GTTTATTTAAGCCTATTTAAGTTAGGTTTAGGCAGTCATGAAGTTGCACCAAATGCGAGCAATGGTGCCGTAATTTTGCA
CGCTTATGTGCAACATGCATTTGGTAATTTAGGTTCCTTATTTTTATCTGGCCTTATTTTCCTTGCTTGTATGGTCACGG
CTATTGGCTTGACCTGCGCATGTGCAGAATATTTTGCTCAGTTAACTAACTTGCCTTACAAATTATTTGTATTTGTTTTA
GTTGCTTTTTCATTACTGATATCGAATCTAGGTTTAACCAAGCTCATTACAGTATCCGTGCCTGTATTGACTGCAATCTA
TCCGCCTGCAATCGTTGTCATCATGCTCAGTTTCTTCTGGAAATTTTGGAATGTACCATCTCGCGTTGTTGGCCCGGTTA
CCGCTATAGCATTGTTGTTTGGTGTGATTGAAGGCTTGAAAGTCGCTGGTTTTACACAGATTCTGCCAAATTTTGTGCAG
CATTTGCCGCTTAATGAACAAAATTTAGCTTGGCTGATTCCATCACTTGTCGTTTTAGTCATTTCTATTGTGATCGATAA
AGTTAGAAAACCAAACGTTTAA

Upstream 100 bases:

>100_bases
TCTTATCCCAAGCAAAATACTTTTTTACCTCTCTAGACTTCACAAAAATGTCATCATTTCAACTAAAATTCGCGCTTCTT
TATTGCGTCTGTCCCTTTTT

Downstream 100 bases:

>100_bases
AACTTATCGTTTTTATAAAGTTGAGCGCTTTATCCAAGCCTGATTTTATACAATAAAAATGCCGTTGTTTTTACCCAAAA
CTGGCATTTTTCTTTTTTTG

Product: branched chain amino acid transporter

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: LIV-III [H]

Number of amino acids: Translated: 433; Mature: 432

Protein sequence:

>433_residues
MTSLRTSDIIALGFMTFALFIGAGNIIFPPIVAQQAGEHVWLAAAGFLITAVGLPVITIMALSRMEGSIAMISSPLGRFF
SLLLTIVCYLSVGPLFATPRTATVSYEIGFSSYFGTSSGSLFVYSLIYFSIVTLVSLYPNKLLDTVGHVLAPLKIIALAI
LGIAAVLIPSNYIPPAINNYVENPVSEGFVNGYLTMDTLGALVFGIVIIQAIYSRGVRDPKLVTKYAIIASIISGIGLTL
VYLSLFKLGLGSHEVAPNASNGAVILHAYVQHAFGNLGSLFLSGLIFLACMVTAIGLTCACAEYFAQLTNLPYKLFVFVL
VAFSLLISNLGLTKLITVSVPVLTAIYPPAIVVIMLSFFWKFWNVPSRVVGPVTAIALLFGVIEGLKVAGFTQILPNFVQ
HLPLNEQNLAWLIPSLVVLVISIVIDKVRKPNV

Sequences:

>Translated_433_residues
MTSLRTSDIIALGFMTFALFIGAGNIIFPPIVAQQAGEHVWLAAAGFLITAVGLPVITIMALSRMEGSIAMISSPLGRFF
SLLLTIVCYLSVGPLFATPRTATVSYEIGFSSYFGTSSGSLFVYSLIYFSIVTLVSLYPNKLLDTVGHVLAPLKIIALAI
LGIAAVLIPSNYIPPAINNYVENPVSEGFVNGYLTMDTLGALVFGIVIIQAIYSRGVRDPKLVTKYAIIASIISGIGLTL
VYLSLFKLGLGSHEVAPNASNGAVILHAYVQHAFGNLGSLFLSGLIFLACMVTAIGLTCACAEYFAQLTNLPYKLFVFVL
VAFSLLISNLGLTKLITVSVPVLTAIYPPAIVVIMLSFFWKFWNVPSRVVGPVTAIALLFGVIEGLKVAGFTQILPNFVQ
HLPLNEQNLAWLIPSLVVLVISIVIDKVRKPNV
>Mature_432_residues
TSLRTSDIIALGFMTFALFIGAGNIIFPPIVAQQAGEHVWLAAAGFLITAVGLPVITIMALSRMEGSIAMISSPLGRFFS
LLLTIVCYLSVGPLFATPRTATVSYEIGFSSYFGTSSGSLFVYSLIYFSIVTLVSLYPNKLLDTVGHVLAPLKIIALAIL
GIAAVLIPSNYIPPAINNYVENPVSEGFVNGYLTMDTLGALVFGIVIIQAIYSRGVRDPKLVTKYAIIASIISGIGLTLV
YLSLFKLGLGSHEVAPNASNGAVILHAYVQHAFGNLGSLFLSGLIFLACMVTAIGLTCACAEYFAQLTNLPYKLFVFVLV
AFSLLISNLGLTKLITVSVPVLTAIYPPAIVVIMLSFFWKFWNVPSRVVGPVTAIALLFGVIEGLKVAGFTQILPNFVQH
LPLNEQNLAWLIPSLVVLVISIVIDKVRKPNV

Specific function: Component of the LIV-III transport system for branched- chain amino acids. BraZ is specific for isoleucine and valine. The LIV-III transport system may be H(+)-coupled [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=431, Percent_Identity=58.7006960556845, Blast_Score=517, Evalue=1e-148,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 46367; Mature: 46236

Theoretical pI: Translated: 8.98; Mature: 8.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSLRTSDIIALGFMTFALFIGAGNIIFPPIVAQQAGEHVWLAAAGFLITAVGLPVITIM
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCEEHHHHHHHHHHHHHHHHHHHH
ALSRMEGSIAMISSPLGRFFSLLLTIVCYLSVGPLFATPRTATVSYEIGFSSYFGTSSGS
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCHHHCCCCCCH
LFVYSLIYFSIVTLVSLYPNKLLDTVGHVLAPLKIIALAILGIAAVLIPSNYIPPAINNY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
VENPVSEGFVNGYLTMDTLGALVFGIVIIQAIYSRGVRDPKLVTKYAIIASIISGIGLTL
HCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
VYLSLFKLGLGSHEVAPNASNGAVILHAYVQHAFGNLGSLFLSGLIFLACMVTAIGLTCA
HHHHHHHHCCCCCCCCCCCCCCEEHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
CAEYFAQLTNLPYKLFVFVLVAFSLLISNLGLTKLITVSVPVLTAIYPPAIVVIMLSFFW
HHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KFWNVPSRVVGPVTAIALLFGVIEGLKVAGFTQILPNFVQHLPLNEQNLAWLIPSLVVLV
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
ISIVIDKVRKPNV
HHHHHHHHCCCCC
>Mature Secondary Structure 
TSLRTSDIIALGFMTFALFIGAGNIIFPPIVAQQAGEHVWLAAAGFLITAVGLPVITIM
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCEEHHHHHHHHHHHHHHHHHHHH
ALSRMEGSIAMISSPLGRFFSLLLTIVCYLSVGPLFATPRTATVSYEIGFSSYFGTSSGS
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCHHHCCCCCCH
LFVYSLIYFSIVTLVSLYPNKLLDTVGHVLAPLKIIALAILGIAAVLIPSNYIPPAINNY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
VENPVSEGFVNGYLTMDTLGALVFGIVIIQAIYSRGVRDPKLVTKYAIIASIISGIGLTL
HCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
VYLSLFKLGLGSHEVAPNASNGAVILHAYVQHAFGNLGSLFLSGLIFLACMVTAIGLTCA
HHHHHHHHCCCCCCCCCCCCCCEEHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
CAEYFAQLTNLPYKLFVFVLVAFSLLISNLGLTKLITVSVPVLTAIYPPAIVVIMLSFFW
HHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KFWNVPSRVVGPVTAIALLFGVIEGLKVAGFTQILPNFVQHLPLNEQNLAWLIPSLVVLV
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
ISIVIDKVRKPNV
HHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1900503; 10984043 [H]