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The map label for this gene is prpC [H]

Identifier: 161349960

GI number: 161349960

Start: 2695966

End: 2697123

Strand: Reverse

Name: prpC [H]

Synonym: ACIAD2756

Alternate gene names: 161349960

Gene position: 2697123-2695966 (Counterclockwise)

Preceding gene: 50085814

Following gene: 50085812

Centisome position: 74.95

GC content: 48.96

Gene sequence:

>1158_bases
ATGAGCTCAAATGAAACAACAACAGGCTTTAAACCAAAAAAATCAGTTGCACTTAGCGGACAAGTGGCAGGCAACACCGC
ACTCTGTACCGTTGGCCGTAGCGGTAACGACTTGCATTATCGTGGCTATGACATTTTAGATCTCGCTGTAGGCAGCCAGT
TTGAAGAAGTGGCACACTTGCTGGTACATGGCAAATTACCAAATCAAGCTGAGCTTAAAGCGTATAAAGCCAAATTAAAG
GCACTTCGTGGCTTACCGGCAGCACTCAAAACAGCACTTGAACAACTTCCGCCGTCTGCTCACCCCATGGATGTGATGCG
TACGGGCGTGTCGGTATTGGGCTGTTTAACCCCAGAACATGAAGACCATAACGAAGCTGGCGCAAAAGACATTGCCGATA
AACTTATGGCCAGCCTCGGTTCGATGCTGCTGTACTGGTATCACTTTAGCAACAATGGGCGCCGTATTGAGGTCGAAACC
GACGACGACTCAATCGCAGCGCATTTCCTGCACTTGTTGCATGGCGAAAAACCATCTGAAGAATGGATTCAGGCTATGCA
CACCTCACTCATTTTGTATGCAGAGCACGAGTTTAATGCATCTACCTTTACCTCACGCGTAGTCGCCGGTACAGGTTCAG
ATATGTACTCGGCCATTACCGGTGGAATTGGCGCACTGCGTGGCCCTAAACACGGCGGTGCCAATGAAGTGGCCTTTGTG
ATTCAGCAACGTTATGACAATGCAGACGAAGCTGAGGCAGACATTCGAAGCCGTGTAGAGAAGAAAGAAGTGGTGATCGG
ATTTGGTCATCCGGTTTATACCGTATCCGACCCGCGTAATGAAGTGATTAAAAAAGTGGCCTACGATTTGGCGCAGTCCC
AGCAAAACACCAAGATGTATTTGATCGCGGAGCGTTTGGAAGCCGTGATGAAAGAAGTAAAAAACATGTTTCCAAACCTC
GACTGGTTTAGTGCGGTAAGTTACCACCTGATGGGTGTGCCAACAGCCATGTTTACACCATTGTTCGTGATTGCACGTAC
CGCAGGGTGGTCGGCGCACGTGATTGAGCAGCGTCAAGACGGCAAGATTATTCGTCCAAGTGCGAACTATACCGGCCCAG
AAAATCTCGAATTTAAACCGTTGGCGGAGCGTGGCTAA

Upstream 100 bases:

>100_bases
GGCGCAAGGCGAAGTGGTTAATCTCGAAATAAGAAGTGAAAATGGTACGACTCCATCGTACCTAATAAATAAATTCAGAT
CGATAGGAAAGGACAATCTT

Downstream 100 bases:

>100_bases
TGTCAAATCCATATCGTAAAACGTTGCCGAATGCTGCACTTGGCGATACCCCGCTGGACTATTACGACGTGCGTCAAGCC
GTTGAAGATATTCTGCCTGG

Product: methylcitrate synthase

Products: NA

Alternate protein names: Citrate synthase 2; Methylcitrate synthase [H]

Number of amino acids: Translated: 385; Mature: 384

Protein sequence:

>385_residues
MSSNETTTGFKPKKSVALSGQVAGNTALCTVGRSGNDLHYRGYDILDLAVGSQFEEVAHLLVHGKLPNQAELKAYKAKLK
ALRGLPAALKTALEQLPPSAHPMDVMRTGVSVLGCLTPEHEDHNEAGAKDIADKLMASLGSMLLYWYHFSNNGRRIEVET
DDDSIAAHFLHLLHGEKPSEEWIQAMHTSLILYAEHEFNASTFTSRVVAGTGSDMYSAITGGIGALRGPKHGGANEVAFV
IQQRYDNADEAEADIRSRVEKKEVVIGFGHPVYTVSDPRNEVIKKVAYDLAQSQQNTKMYLIAERLEAVMKEVKNMFPNL
DWFSAVSYHLMGVPTAMFTPLFVIARTAGWSAHVIEQRQDGKIIRPSANYTGPENLEFKPLAERG

Sequences:

>Translated_385_residues
MSSNETTTGFKPKKSVALSGQVAGNTALCTVGRSGNDLHYRGYDILDLAVGSQFEEVAHLLVHGKLPNQAELKAYKAKLK
ALRGLPAALKTALEQLPPSAHPMDVMRTGVSVLGCLTPEHEDHNEAGAKDIADKLMASLGSMLLYWYHFSNNGRRIEVET
DDDSIAAHFLHLLHGEKPSEEWIQAMHTSLILYAEHEFNASTFTSRVVAGTGSDMYSAITGGIGALRGPKHGGANEVAFV
IQQRYDNADEAEADIRSRVEKKEVVIGFGHPVYTVSDPRNEVIKKVAYDLAQSQQNTKMYLIAERLEAVMKEVKNMFPNL
DWFSAVSYHLMGVPTAMFTPLFVIARTAGWSAHVIEQRQDGKIIRPSANYTGPENLEFKPLAERG
>Mature_384_residues
SSNETTTGFKPKKSVALSGQVAGNTALCTVGRSGNDLHYRGYDILDLAVGSQFEEVAHLLVHGKLPNQAELKAYKAKLKA
LRGLPAALKTALEQLPPSAHPMDVMRTGVSVLGCLTPEHEDHNEAGAKDIADKLMASLGSMLLYWYHFSNNGRRIEVETD
DDSIAAHFLHLLHGEKPSEEWIQAMHTSLILYAEHEFNASTFTSRVVAGTGSDMYSAITGGIGALRGPKHGGANEVAFVI
QQRYDNADEAEADIRSRVEKKEVVIGFGHPVYTVSDPRNEVIKKVAYDLAQSQQNTKMYLIAERLEAVMKEVKNMFPNLD
WFSAVSYHLMGVPTAMFTPLFVIARTAGWSAHVIEQRQDGKIIRPSANYTGPENLEFKPLAERG

Specific function: Catalyzes the synthesis of 2-methylcitrate from propionyl-CoA and oxaloacetate. Also catalyzes the condensation of oxaloacetate with acetyl-CoA but with a lower specificity [H]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786527, Length=379, Percent_Identity=74.6701846965699, Blast_Score=581, Evalue=1e-167,
Organism=Escherichia coli, GI1786939, Length=369, Percent_Identity=31.4363143631436, Blast_Score=167, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17555174, Length=369, Percent_Identity=23.5772357723577, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6319850, Length=368, Percent_Identity=25.8152173913043, Blast_Score=91, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6324328, Length=345, Percent_Identity=26.0869565217391, Blast_Score=81, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6325257, Length=392, Percent_Identity=26.0204081632653, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24640124, Length=341, Percent_Identity=24.3401759530792, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24640126, Length=336, Percent_Identity=24.4047619047619, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI21356863, Length=378, Percent_Identity=23.015873015873, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011278
- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.5 [H]

Molecular weight: Translated: 42335; Mature: 42203

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSNETTTGFKPKKSVALSGQVAGNTALCTVGRSGNDLHYRGYDILDLAVGSQFEEVAHL
CCCCCCCCCCCCCCCEEEECEECCCEEEEEECCCCCCCEECCCEEEEHHHCCCHHHHHHH
LVHGKLPNQAELKAYKAKLKALRGLPAALKTALEQLPPSAHPMDVMRTGVSVLGCLTPEH
HHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCC
EDHNEAGAKDIADKLMASLGSMLLYWYHFSNNGRRIEVETDDDSIAAHFLHLLHGEKPSE
CCCCCCCHHHHHHHHHHHHHHHHEEEEEECCCCEEEEEECCCHHHHHHHHHHHCCCCCHH
EWIQAMHTSLILYAEHEFNASTFTSRVVAGTGSDMYSAITGGIGALRGPKHGGANEVAFV
HHHHHHHHHEEEEEECCCCCHHHHHHHEECCCCHHHHHHHCCHHHCCCCCCCCCHHHHHH
IQQRYDNADEAEADIRSRVEKKEVVIGFGHPVYTVSDPRNEVIKKVAYDLAQSQQNTKMY
HHHHCCCCCHHHHHHHHHHHHCCEEEECCCEEEEECCCHHHHHHHHHHHHHHCCCCCEEE
LIAERLEAVMKEVKNMFPNLDWFSAVSYHLMGVPTAMFTPLFVIARTAGWSAHVIEQRQD
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHCCC
GKIIRPSANYTGPENLEFKPLAERG
CCEECCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SSNETTTGFKPKKSVALSGQVAGNTALCTVGRSGNDLHYRGYDILDLAVGSQFEEVAHL
CCCCCCCCCCCCCCEEEECEECCCEEEEEECCCCCCCEECCCEEEEHHHCCCHHHHHHH
LVHGKLPNQAELKAYKAKLKALRGLPAALKTALEQLPPSAHPMDVMRTGVSVLGCLTPEH
HHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCC
EDHNEAGAKDIADKLMASLGSMLLYWYHFSNNGRRIEVETDDDSIAAHFLHLLHGEKPSE
CCCCCCCHHHHHHHHHHHHHHHHEEEEEECCCCEEEEEECCCHHHHHHHHHHHCCCCCHH
EWIQAMHTSLILYAEHEFNASTFTSRVVAGTGSDMYSAITGGIGALRGPKHGGANEVAFV
HHHHHHHHHEEEEEECCCCCHHHHHHHEECCCCHHHHHHHCCHHHCCCCCCCCCHHHHHH
IQQRYDNADEAEADIRSRVEKKEVVIGFGHPVYTVSDPRNEVIKKVAYDLAQSQQNTKMY
HHHHCCCCCHHHHHHHHHHHHCCEEEECCCEEEEECCCHHHHHHHHHHHHHHCCCCCEEE
LIAERLEAVMKEVKNMFPNLDWFSAVSYHLMGVPTAMFTPLFVIARTAGWSAHVIEQRQD
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHCCC
GKIIRPSANYTGPENLEFKPLAERG
CCEECCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 8508809; 9325432 [H]