Click here to switch to the map view.

The map label for this gene is prpB [H]

Identifier: 50085814

GI number: 50085814

Start: 2697340

End: 2698224

Strand: Reverse

Name: prpB [H]

Synonym: ACIAD2758

Alternate gene names: 50085814

Gene position: 2698224-2697340 (Counterclockwise)

Preceding gene: 50085815

Following gene: 161349960

Centisome position: 74.98

GC content: 46.78

Gene sequence:

>885_bases
ATGTCTACAGTATCAGCAGGACAGAAATTTAGAGACGCGGTCGCCAGTGAACGCCCATTACAGGTGGTGGGTGCCATCAA
TGCCAACCATGCCTTATTGGCCAAACGTGCAGGCTATAAAGCCATTTATTTGTCGGGTGGTGGGGTTGCGGCGGGTTCGC
TTGGTTTACCCGATTTAGGCATTAGCAATTTAGATGATGTATTGATCGATGTGCGCCGTATTACGGATGTATGCGATTTG
CCTTTGCTGGTCGATGCCGATACAGGCTTTGGCGCTTCAGCATTTAATATTGCCCGTACTACTAAATCGCTGATTAAATT
TGGTGCTGCTGCCATGCACATTGAAGATCAGGTCGGTGCCAAGCGTTGTGGTCATCGTCCAAACAAAGCCATTGTGAGCC
AAGAAGAAATGGTCGATCGGATTAAAGCGGCGGTTGATGCGCGTACCGATGAAAATTTTGTGATTATGGCACGTACCGAT
GCATTGGCTGTCGATGGTTTGCAAGCTGCAATTGACCGTGCAGGCGCGTATATCGAAGCTGGTGCAGACATGCTATTTCC
AGAAGCCATTACCGAGCTTGCAATGTATAAGCAATTTGCACAGGCGACTGGCGCACCCATTTTGGCCAATATTACCGAGT
TTGGTTCTACGCCACTATTTACCACCGATGAACTGGCCTCTGCCGATGTAAGCCTTGCTTTGTATCCACTCTCAGCATTT
CGTGCCATGAACAAGGCAGCCGAAACAGTCTATGAAACCTTACGTAAAGAAGGCACACAAAAGAATGTGGTCGACATCAT
GCAAACGCGTAAAGAGCTATATGAGCGCATTAATTACTATGCGTTTGAAGACTATTTAGACAATGCATTTGCGAAAAAGA
AATAA

Upstream 100 bases:

>100_bases
ATACTGAACTGGCAGAACTTCTGATGCGCAGGCATATCGCAGCATCGTATCGCAACATCGAACAACATTTTATTTAATTG
ACAGGGAGAGAAACCAGCAC

Downstream 100 bases:

>100_bases
ATAAGCCCTCTTTGAAAATAAGCGGAAACGATGGTCTTGGCCATCGTGAGGGCGGCATGGATGCAGCTACATTGGACGGC
CTTGCATTGCTATCAAAGAG

Product: 2-methylisocitrate lyase

Products: NA

Alternate protein names: 2-methylisocitrate lyase [H]

Number of amino acids: Translated: 294; Mature: 293

Protein sequence:

>294_residues
MSTVSAGQKFRDAVASERPLQVVGAINANHALLAKRAGYKAIYLSGGGVAAGSLGLPDLGISNLDDVLIDVRRITDVCDL
PLLVDADTGFGASAFNIARTTKSLIKFGAAAMHIEDQVGAKRCGHRPNKAIVSQEEMVDRIKAAVDARTDENFVIMARTD
ALAVDGLQAAIDRAGAYIEAGADMLFPEAITELAMYKQFAQATGAPILANITEFGSTPLFTTDELASADVSLALYPLSAF
RAMNKAAETVYETLRKEGTQKNVVDIMQTRKELYERINYYAFEDYLDNAFAKKK

Sequences:

>Translated_294_residues
MSTVSAGQKFRDAVASERPLQVVGAINANHALLAKRAGYKAIYLSGGGVAAGSLGLPDLGISNLDDVLIDVRRITDVCDL
PLLVDADTGFGASAFNIARTTKSLIKFGAAAMHIEDQVGAKRCGHRPNKAIVSQEEMVDRIKAAVDARTDENFVIMARTD
ALAVDGLQAAIDRAGAYIEAGADMLFPEAITELAMYKQFAQATGAPILANITEFGSTPLFTTDELASADVSLALYPLSAF
RAMNKAAETVYETLRKEGTQKNVVDIMQTRKELYERINYYAFEDYLDNAFAKKK
>Mature_293_residues
STVSAGQKFRDAVASERPLQVVGAINANHALLAKRAGYKAIYLSGGGVAAGSLGLPDLGISNLDDVLIDVRRITDVCDLP
LLVDADTGFGASAFNIARTTKSLIKFGAAAMHIEDQVGAKRCGHRPNKAIVSQEEMVDRIKAAVDARTDENFVIMARTDA
LAVDGLQAAIDRAGAYIEAGADMLFPEAITELAMYKQFAQATGAPILANITEFGSTPLFTTDELASADVSLALYPLSAFR
AMNKAAETVYETLRKEGTQKNVVDIMQTRKELYERINYYAFEDYLDNAFAKKK

Specific function: Catalyzes the formation of pyruvate and succinate from 2-methylisocitrate [H]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Methylisocitrate lyase family [H]

Homologues:

Organism=Escherichia coli, GI1786525, Length=294, Percent_Identity=77.891156462585, Blast_Score=480, Evalue=1e-137,
Organism=Escherichia coli, GI1790445, Length=256, Percent_Identity=28.90625, Blast_Score=78, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI71982926, Length=203, Percent_Identity=32.512315270936, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17561814, Length=203, Percent_Identity=32.512315270936, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR012695
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00463 ICL [H]

EC number: =4.1.3.30 [H]

Molecular weight: Translated: 31682; Mature: 31551

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00161 ISOCITRATE_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTVSAGQKFRDAVASERPLQVVGAINANHALLAKRAGYKAIYLSGGGVAAGSLGLPDLG
CCCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHCCCEEEEEECCCEEECCCCCCCCC
ISNLDDVLIDVRRITDVCDLPLLVDADTGFGASAFNIARTTKSLIKFGAAAMHIEDQVGA
CCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHEEHHHHHHH
KRCGHRPNKAIVSQEEMVDRIKAAVDARTDENFVIMARTDALAVDGLQAAIDRAGAYIEA
HHCCCCCCHHHCCHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHCCCEEEC
GADMLFPEAITELAMYKQFAQATGAPILANITEFGSTPLFTTDELASADVSLALYPLSAF
CCCHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHCCCCCCCCHHHHCCCCEEEEHHHHHH
RAMNKAAETVYETLRKEGTQKNVVDIMQTRKELYERINYYAFEDYLDNAFAKKK
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
STVSAGQKFRDAVASERPLQVVGAINANHALLAKRAGYKAIYLSGGGVAAGSLGLPDLG
CCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHCCCEEEEEECCCEEECCCCCCCCC
ISNLDDVLIDVRRITDVCDLPLLVDADTGFGASAFNIARTTKSLIKFGAAAMHIEDQVGA
CCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHEEHHHHHHH
KRCGHRPNKAIVSQEEMVDRIKAAVDARTDENFVIMARTDALAVDGLQAAIDRAGAYIEA
HHCCCCCCHHHCCHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHCCCEEEC
GADMLFPEAITELAMYKQFAQATGAPILANITEFGSTPLFTTDELASADVSLALYPLSAF
CCCHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHCCCCCCCCHHHHCCCCEEEEHHHHHH
RAMNKAAETVYETLRKEGTQKNVVDIMQTRKELYERINYYAFEDYLDNAFAKKK
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9006051; 11677609; 10482501; 14575713 [H]