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The map label for this gene is argH

Identifier: 49481364

GI number: 49481364

Start: 4427009

End: 4428400

Strand: Reverse

Name: argH

Synonym: BT9727_4363

Alternate gene names: 49481364

Gene position: 4428400-4427009 (Counterclockwise)

Preceding gene: 49481365

Following gene: 49480696

Centisome position: 84.55

GC content: 38.22

Gene sequence:

>1392_bases
GTGAGCAAACTTTGGGGCGGACGTTTTACAGAAGAAGCGGAAGCGTGGGTTGAAGAGTTCGGAGCATCCATCTCCTTCGA
TCAACAATTAGTAAATCAAGATATAAACGGAAGTATTGCACACGTAACGATGCTAGCAAAGCAAGGCATCGTTACGAAAG
AAGAAGCAGAGAAAATAAAGATAGGTCTTCAATATTTATTAGAAGAAGCGAAACAAAATAAATTGCATTTTTCGGTTGAA
GCGGAAGACATTCATTTAAATATCGAAAAGATGTTAATTGAAAAAATCGGTGAAGTAGGAGGGAAACTTCATACTGGCCG
AAGTCGTAATGATCAAGTAGCGACAGATATGCACTTATATTTAAAAGAAAAAGTAGAGCATATTATGAAAGCTACAAAAC
AATTGCAGACGGTTCTTGTTCATCAAGCAGAAAATAATATTGAAACCATTATGCCTGGCTATACGCACTTACAGCGTGCC
CAGCCAATTTCATTTGCTCATCATATTCTCGCTTACTTTTGGATGTTAGAGCGTGATGTAAATCGTTATGAAGATTCATT
AAAGCGTATTAACATTTCGCCATTAGGAGCAGGTGCGTTAGCTGGAACGACATTTCCAATTGACCGAGAATATAGTGCGG
AGCTTCTTGGATTTAATGGAATCTATGAAAATAGTTTAGATGCAGTAAGCGATCGTGATTTCATACTGGAGTTCCTCAGT
AACTCATCTATGCTTATGATGCACTTATCACGCTTTTGCGAAGAACTTATTTTATGGAGTAGCCAAGAGTTTCAATTTAT
TGAAATGAGCGATCAATACGCAACTGGAAGCAGCATTATGCCGCAAAAGAAAAATCCAGATATGGCGGAACTTATTCGTG
GTAAAACAGGCAGAGTGTACGGTAATTTATTTAGTTTACTTACAGTCATGAAAGGATTACCGCTCGCTTATAATAAAGAT
TTGCAAGAAGATAAAGAAGGAATGTTTGATACAGTGAAAACAGTAGAAGGCTGCCTTCATATTATGGCAGGCATGTTAGA
GACAATGACTGTAAACAAAGAAAAAATGGGGCAAGCTGTAACGCAAGATTTCTCTAACGCAACAGAAATTGCTGACTACT
TAGCAAACAAAGGACTACCATTCCGCCAAGCTCATGAAATTGTTGGAAAGCTAGTGCTACACTGCACACAAAAAGGAATT
TATTTAATAGATGTACCACTTGCAACATATAAAGAAATGAGCGCGCTATTTGAAGGAGATTTGTATGAAGTTCTGTCACC
ATATGCAGCTGTAAAGCGTCGTAACAGTGCTGGTGGGACTGGGTTTGAGCAAATTGAAAAAGCTTTGGAGAAGGCGAAGG
GATTAGTTGGAGAGATTGTAGGAATTAAATGA

Upstream 100 bases:

>100_bases
CTCAGGATGAATTTAATCATGATGCAGCAGTTGGGTTCATTTCATTATTCGGTTTACCGACGAAAGTATACAGCCAAGTG
AATCAAAAGAAGGTGGAAGC

Downstream 100 bases:

>100_bases
TGTGTGAGATAGAAGGTAAGATGCTTTTTATAGAAAGCACCTTACCTTTTTGTATTTAGATTTTTACAAGAGTATTTATT
ATTTCATACTCATAAAATGA

Product: argininosuccinate lyase

Products: NA

Alternate protein names: ASAL; Arginosuccinase [H]

Number of amino acids: Translated: 463; Mature: 462

Protein sequence:

>463_residues
MSKLWGGRFTEEAEAWVEEFGASISFDQQLVNQDINGSIAHVTMLAKQGIVTKEEAEKIKIGLQYLLEEAKQNKLHFSVE
AEDIHLNIEKMLIEKIGEVGGKLHTGRSRNDQVATDMHLYLKEKVEHIMKATKQLQTVLVHQAENNIETIMPGYTHLQRA
QPISFAHHILAYFWMLERDVNRYEDSLKRINISPLGAGALAGTTFPIDREYSAELLGFNGIYENSLDAVSDRDFILEFLS
NSSMLMMHLSRFCEELILWSSQEFQFIEMSDQYATGSSIMPQKKNPDMAELIRGKTGRVYGNLFSLLTVMKGLPLAYNKD
LQEDKEGMFDTVKTVEGCLHIMAGMLETMTVNKEKMGQAVTQDFSNATEIADYLANKGLPFRQAHEIVGKLVLHCTQKGI
YLIDVPLATYKEMSALFEGDLYEVLSPYAAVKRRNSAGGTGFEQIEKALEKAKGLVGEIVGIK

Sequences:

>Translated_463_residues
MSKLWGGRFTEEAEAWVEEFGASISFDQQLVNQDINGSIAHVTMLAKQGIVTKEEAEKIKIGLQYLLEEAKQNKLHFSVE
AEDIHLNIEKMLIEKIGEVGGKLHTGRSRNDQVATDMHLYLKEKVEHIMKATKQLQTVLVHQAENNIETIMPGYTHLQRA
QPISFAHHILAYFWMLERDVNRYEDSLKRINISPLGAGALAGTTFPIDREYSAELLGFNGIYENSLDAVSDRDFILEFLS
NSSMLMMHLSRFCEELILWSSQEFQFIEMSDQYATGSSIMPQKKNPDMAELIRGKTGRVYGNLFSLLTVMKGLPLAYNKD
LQEDKEGMFDTVKTVEGCLHIMAGMLETMTVNKEKMGQAVTQDFSNATEIADYLANKGLPFRQAHEIVGKLVLHCTQKGI
YLIDVPLATYKEMSALFEGDLYEVLSPYAAVKRRNSAGGTGFEQIEKALEKAKGLVGEIVGIK
>Mature_462_residues
SKLWGGRFTEEAEAWVEEFGASISFDQQLVNQDINGSIAHVTMLAKQGIVTKEEAEKIKIGLQYLLEEAKQNKLHFSVEA
EDIHLNIEKMLIEKIGEVGGKLHTGRSRNDQVATDMHLYLKEKVEHIMKATKQLQTVLVHQAENNIETIMPGYTHLQRAQ
PISFAHHILAYFWMLERDVNRYEDSLKRINISPLGAGALAGTTFPIDREYSAELLGFNGIYENSLDAVSDRDFILEFLSN
SSMLMMHLSRFCEELILWSSQEFQFIEMSDQYATGSSIMPQKKNPDMAELIRGKTGRVYGNLFSLLTVMKGLPLAYNKDL
QEDKEGMFDTVKTVEGCLHIMAGMLETMTVNKEKMGQAVTQDFSNATEIADYLANKGLPFRQAHEIVGKLVLHCTQKGIY
LIDVPLATYKEMSALFEGDLYEVLSPYAAVKRRNSAGGTGFEQIEKALEKAKGLVGEIVGIK

Specific function: Arginine biosynthesis; eighth (last) step. [C]

COG id: COG0165

COG function: function code E; Argininosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Argininosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI31541964, Length=454, Percent_Identity=44.4933920704846, Blast_Score=397, Evalue=1e-110,
Organism=Homo sapiens, GI68303542, Length=454, Percent_Identity=44.4933920704846, Blast_Score=397, Evalue=1e-110,
Organism=Homo sapiens, GI68303549, Length=454, Percent_Identity=42.0704845814978, Blast_Score=364, Evalue=1e-101,
Organism=Homo sapiens, GI68303547, Length=454, Percent_Identity=41.8502202643172, Blast_Score=362, Evalue=1e-100,
Organism=Escherichia coli, GI1790398, Length=455, Percent_Identity=49.010989010989, Blast_Score=465, Evalue=1e-132,
Organism=Caenorhabditis elegans, GI17553882, Length=226, Percent_Identity=27.8761061946903, Blast_Score=69, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6321806, Length=454, Percent_Identity=46.0352422907489, Blast_Score=398, Evalue=1e-111,
Organism=Drosophila melanogaster, GI221473854, Length=451, Percent_Identity=40.1330376940133, Blast_Score=360, Evalue=1e-99,
Organism=Drosophila melanogaster, GI78706858, Length=451, Percent_Identity=40.1330376940133, Blast_Score=360, Evalue=1e-99,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009049
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF00206 Lyase_1 [H]

EC number: =4.3.2.1 [H]

Molecular weight: Translated: 52093; Mature: 51961

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLWGGRFTEEAEAWVEEFGASISFDQQLVNQDINGSIAHVTMLAKQGIVTKEEAEKIK
CCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHCCCCCCCHHHHHHHHHCCCCCHHHHHHHH
IGLQYLLEEAKQNKLHFSVEAEDIHLNIEKMLIEKIGEVGGKLHTGRSRNDQVATDMHLY
HHHHHHHHHHHCCCEEEEEECCCEEEHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
LKEKVEHIMKATKQLQTVLVHQAENNIETIMPGYTHLQRAQPISFAHHILAYFWMLERDV
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHHHH
NRYEDSLKRINISPLGAGALAGTTFPIDREYSAELLGFNGIYENSLDAVSDRDFILEFLS
HHHHHHHHEECCCCCCCCHHCCCCCCCCCCCCHHHHCCCCCCCCCCHHHCHHHHHHHHHC
NSSMLMMHLSRFCEELILWSSQEFQFIEMSDQYATGSSIMPQKKNPDMAELIRGKTGRVY
CCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHCCCCCHHH
GNLFSLLTVMKGLPLAYNKDLQEDKEGMFDTVKTVEGCLHIMAGMLETMTVNKEKMGQAV
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
TQDFSNATEIADYLANKGLPFRQAHEIVGKLVLHCTQKGIYLIDVPLATYKEMSALFEGD
HHHHCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHCCC
LYEVLSPYAAVKRRNSAGGTGFEQIEKALEKAKGLVGEIVGIK
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SKLWGGRFTEEAEAWVEEFGASISFDQQLVNQDINGSIAHVTMLAKQGIVTKEEAEKIK
CCCCCCCCCHHHHHHHHHHCCCCCHHHHHHCCCCCCCHHHHHHHHHCCCCCHHHHHHHH
IGLQYLLEEAKQNKLHFSVEAEDIHLNIEKMLIEKIGEVGGKLHTGRSRNDQVATDMHLY
HHHHHHHHHHHCCCEEEEEECCCEEEHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
LKEKVEHIMKATKQLQTVLVHQAENNIETIMPGYTHLQRAQPISFAHHILAYFWMLERDV
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHHHH
NRYEDSLKRINISPLGAGALAGTTFPIDREYSAELLGFNGIYENSLDAVSDRDFILEFLS
HHHHHHHHEECCCCCCCCHHCCCCCCCCCCCCHHHHCCCCCCCCCCHHHCHHHHHHHHHC
NSSMLMMHLSRFCEELILWSSQEFQFIEMSDQYATGSSIMPQKKNPDMAELIRGKTGRVY
CCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHCCCCCHHH
GNLFSLLTVMKGLPLAYNKDLQEDKEGMFDTVKTVEGCLHIMAGMLETMTVNKEKMGQAV
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
TQDFSNATEIADYLANKGLPFRQAHEIVGKLVLHCTQKGIYLIDVPLATYKEMSALFEGD
HHHHCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHCCC
LYEVLSPYAAVKRRNSAGGTGFEQIEKALEKAKGLVGEIVGIK
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA