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The map label for this gene is argG

Identifier: 49481365

GI number: 49481365

Start: 4428397

End: 4429602

Strand: Reverse

Name: argG

Synonym: BT9727_4364

Alternate gene names: 49481365

Gene position: 4429602-4428397 (Counterclockwise)

Preceding gene: 49480698

Following gene: 49481364

Centisome position: 84.57

GC content: 37.81

Gene sequence:

>1206_bases
ATGGATAAGAAAAAAGTTGTATTAGCATATTCCGGAGGTCTTGATACTTCCGTTGCAATTAAATGGTTACAAGAGAAGAA
TTATGATATTATCGCGCTTTGTTTAGACTTAGGGGAAGGTAAAGACTTAGCATTTGTAAAAGAAAAGGCACTTTCAGTAG
GTGCAATTAAATCATATATGATTGATGTTCAAGAAGAATTTGCAAACGAATATGCATTGATGGCGATGCAAGCACACACG
TTATACGAAGGGAAATATCCTCTCGTTTCGGCGTTATCTCGTCCACTTATCGCAAAGAAATTAGTAGAAATTGCAGAACA
AGAAGGAGCGACTGCAGTTGCACACGGATGTACAGGGAAAGGGAATGATCAAGTTCGTTTTGAAGTTTCTATTCAAGCAT
TAAATCCTTACTTAGAAGTAATTGCACCTGTACGTGAATGGAAATGGTCACGTGAAGAAGAAATTGCCTATGCAAAAGAA
AATAATGTACCGATTCCAATTAATTTAGATAGCCCGTTTTCAATCGATCAAAATTTATGGGGACGCAGCAATGAATGTGG
CATTTTAGAAGATCCATGGGCAGCGCCGCCAGAAGATGCATATGAGATGACATTGGCATTAGAAGACACACCGAATAAAC
CAGAATTTGTAGAAATTGGCTTTGAAGCAGGCGTACCGACTACTTTAAATGGCACTGCATATCCACTTTCAGAACTTATT
AAAACGTTAAATGCACTTGCAGGAAAACATGGCGTTGGACGTATCGATCACGTAGAAAATCGTCTTGTTGGTATTAAATC
ACGTGAAGTATACGAATGCCCAGCAGCAATGACATTAATTACGGCGCATAAAGAACTTGAAGATTTAACACTTGTAAAAG
AAGTAGCACACTTTAAACCGATGATTGAGCAAAAAATAACAGAGCTTATTTATAACGGTTTATGGTTCTCGCCTTTAAAA
CAAGCGCTTCATGCTTTCTTACAAGAAACGCAAAAGAATGTAACAGGCATGGTGCGTGTGAAATTATTTAAAGGTCATGC
AATTGTAGAAGGGCGTAAATCTGAGTACTCTTTATACGATGAAAAATTAGCAACATATACTGCTCAGGATGAATTTAATC
ATGATGCAGCAGTTGGGTTCATTTCATTATTCGGTTTACCGACGAAAGTATACAGCCAAGTGAATCAAAAGAAGGTGGAA
GCGTGA

Upstream 100 bases:

>100_bases
GAAAAAATTTGAAAATGGCTCTTGATACATTTTGTAAAAGGTGTTAAAGTGTGAATTATTATAAAAGAAAATGAATATTT
ATTCTTTAGGGGGATGCAAA

Downstream 100 bases:

>100_bases
GCAAACTTTGGGGCGGACGTTTTACAGAAGAAGCGGAAGCGTGGGTTGAAGAGTTCGGAGCATCCATCTCCTTCGATCAA
CAATTAGTAAATCAAGATAT

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase [H]

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MDKKKVVLAYSGGLDTSVAIKWLQEKNYDIIALCLDLGEGKDLAFVKEKALSVGAIKSYMIDVQEEFANEYALMAMQAHT
LYEGKYPLVSALSRPLIAKKLVEIAEQEGATAVAHGCTGKGNDQVRFEVSIQALNPYLEVIAPVREWKWSREEEIAYAKE
NNVPIPINLDSPFSIDQNLWGRSNECGILEDPWAAPPEDAYEMTLALEDTPNKPEFVEIGFEAGVPTTLNGTAYPLSELI
KTLNALAGKHGVGRIDHVENRLVGIKSREVYECPAAMTLITAHKELEDLTLVKEVAHFKPMIEQKITELIYNGLWFSPLK
QALHAFLQETQKNVTGMVRVKLFKGHAIVEGRKSEYSLYDEKLATYTAQDEFNHDAAVGFISLFGLPTKVYSQVNQKKVE
A

Sequences:

>Translated_401_residues
MDKKKVVLAYSGGLDTSVAIKWLQEKNYDIIALCLDLGEGKDLAFVKEKALSVGAIKSYMIDVQEEFANEYALMAMQAHT
LYEGKYPLVSALSRPLIAKKLVEIAEQEGATAVAHGCTGKGNDQVRFEVSIQALNPYLEVIAPVREWKWSREEEIAYAKE
NNVPIPINLDSPFSIDQNLWGRSNECGILEDPWAAPPEDAYEMTLALEDTPNKPEFVEIGFEAGVPTTLNGTAYPLSELI
KTLNALAGKHGVGRIDHVENRLVGIKSREVYECPAAMTLITAHKELEDLTLVKEVAHFKPMIEQKITELIYNGLWFSPLK
QALHAFLQETQKNVTGMVRVKLFKGHAIVEGRKSEYSLYDEKLATYTAQDEFNHDAAVGFISLFGLPTKVYSQVNQKKVE
A
>Mature_401_residues
MDKKKVVLAYSGGLDTSVAIKWLQEKNYDIIALCLDLGEGKDLAFVKEKALSVGAIKSYMIDVQEEFANEYALMAMQAHT
LYEGKYPLVSALSRPLIAKKLVEIAEQEGATAVAHGCTGKGNDQVRFEVSIQALNPYLEVIAPVREWKWSREEEIAYAKE
NNVPIPINLDSPFSIDQNLWGRSNECGILEDPWAAPPEDAYEMTLALEDTPNKPEFVEIGFEAGVPTTLNGTAYPLSELI
KTLNALAGKHGVGRIDHVENRLVGIKSREVYECPAAMTLITAHKELEDLTLVKEVAHFKPMIEQKITELIYNGLWFSPLK
QALHAFLQETQKNVTGMVRVKLFKGHAIVEGRKSEYSLYDEKLATYTAQDEFNHDAAVGFISLFGLPTKVYSQVNQKKVE
A

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI53759107, Length=409, Percent_Identity=43.520782396088, Blast_Score=311, Evalue=7e-85,
Organism=Homo sapiens, GI16950633, Length=409, Percent_Identity=43.520782396088, Blast_Score=311, Evalue=7e-85,
Organism=Escherichia coli, GI1789563, Length=406, Percent_Identity=26.6009852216749, Blast_Score=117, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6324514, Length=417, Percent_Identity=38.3693045563549, Blast_Score=282, Evalue=8e-77,
Organism=Drosophila melanogaster, GI21358151, Length=398, Percent_Identity=39.6984924623116, Blast_Score=285, Evalue=4e-77,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00764 Arginosuc_synth [H]

EC number: =6.3.4.5 [H]

Molecular weight: Translated: 44710; Mature: 44710

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1 ; PS00565 ARGININOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKKKVVLAYSGGLDTSVAIKWLQEKNYDIIALCLDLGEGKDLAFVKEKALSVGAIKSYM
CCCCEEEEEECCCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
IDVQEEFANEYALMAMQAHTLYEGKYPLVSALSRPLIAKKLVEIAEQEGATAVAHGCTGK
HHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHCHHHHHHHHHHHHHCCCEEEEECCCCC
GNDQVRFEVSIQALNPYLEVIAPVREWKWSREEEIAYAKENNVPIPINLDSPFSIDQNLW
CCCEEEEEEEEEHHHHHHHHHHHHHHHCCCCCHHCEEECCCCCEEEEECCCCCCCCHHHC
GRSNECGILEDPWAAPPEDAYEMTLALEDTPNKPEFVEIGFEAGVPTTLNGTAYPLSELI
CCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCHHHHH
KTLNALAGKHGVGRIDHVENRLVGIKSREVYECPAAMTLITAHKELEDLTLVKEVAHFKP
HHHHHHHCCCCCCCHHHHHHHEECCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
MIEQKITELIYNGLWFSPLKQALHAFLQETQKNVTGMVRVKLFKGHAIVEGRKSEYSLYD
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCEEECCCCCCHHHHH
EKLATYTAQDEFNHDAAVGFISLFGLPTKVYSQVNQKKVEA
HHHHHEECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MDKKKVVLAYSGGLDTSVAIKWLQEKNYDIIALCLDLGEGKDLAFVKEKALSVGAIKSYM
CCCCEEEEEECCCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
IDVQEEFANEYALMAMQAHTLYEGKYPLVSALSRPLIAKKLVEIAEQEGATAVAHGCTGK
HHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHCHHHHHHHHHHHHHCCCEEEEECCCCC
GNDQVRFEVSIQALNPYLEVIAPVREWKWSREEEIAYAKENNVPIPINLDSPFSIDQNLW
CCCEEEEEEEEEHHHHHHHHHHHHHHHCCCCCHHCEEECCCCCEEEEECCCCCCCCHHHC
GRSNECGILEDPWAAPPEDAYEMTLALEDTPNKPEFVEIGFEAGVPTTLNGTAYPLSELI
CCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCHHHHH
KTLNALAGKHGVGRIDHVENRLVGIKSREVYECPAAMTLITAHKELEDLTLVKEVAHFKP
HHHHHHHCCCCCCCHHHHHHHEECCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
MIEQKITELIYNGLWFSPLKQALHAFLQETQKNVTGMVRVKLFKGHAIVEGRKSEYSLYD
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCEEECCCCCCHHHHH
EKLATYTAQDEFNHDAAVGFISLFGLPTKVYSQVNQKKVEA
HHHHHEECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA