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The map label for this gene is 49480949

Identifier: 49480949

GI number: 49480949

Start: 4045593

End: 4046291

Strand: Reverse

Name: 49480949

Synonym: BT9727_3978

Alternate gene names: NA

Gene position: 4046291-4045593 (Counterclockwise)

Preceding gene: 49480955

Following gene: 49478589

Centisome position: 77.25

GC content: 34.62

Gene sequence:

>699_bases
ATGATATATTCATGCTATCCAATCATTATTTTTAGGGAGAATAGGGAAATGACAAACAATAATCAAATAGGTGAAAATAA
GGAACAAACTATTTTTGATCATAAAGGAAATGTAATTATGACAGAAGATAGAGAAATACAAATTATTTCAAAATTCGAAG
AACCTCTTATTGTCGTGTTAGGAAATGTATTAAGTGATGAAGAGTGTGATGAATTAATTGAATTGTCTAAAAATAAATTG
GCACGTTCAAAAGTTGGTTCATCACGTGATGTAAATGATATTCGAACGAGTAGTGGTGCATTTTTGGACGATAATGAACT
TACGGAGAAGATTGAAAAACGAATTTCATCTATCATGAATGTTCCTGCGTCGCATGGAGAAGGCTTACACATTTTAAATT
ATGAAGTGGATCAACAATATAAAGCGCACTATGATTATTTTGCGGAACATAGTAGATCCGCTGCTAATAATCGTATTAGT
ACGCTTGTTATGTACTTAAATGATGTCGAAGAAGGCGGAGAAACGTTCTTTCCGAAATTAAATCTTTCTGTGCACCCTAG
AAAGGGAATGGCAGTATACTTTGAGTATTTCTATCAAGACCAATCATTAAACGAGCTTACGTTACACGGAGGGGCACCTG
TAACGAAAGGCGAGAAATGGATTGCAACGCAGTGGGTGAGAAGAGGTACTTATAAGTAA

Upstream 100 bases:

>100_bases
TGATTTCTCATTTGAAAATAGGCTGTTTTTTGAAAGAAAATGGATAAATAAAACAATTAAAAGTTTAAATTTTCTCAAAA
AAGAGAAAGGTAGAAAGAAT

Downstream 100 bases:

>100_bases
AAGTGTGTATGTGACGTTTTGTTCTTCAATGCATAAGGAGAACAAAACGTTTTTTTATTAAATAAAGGAGCATCTTTCGT
TATAATAACAAGTAATACAT

Product: prolyl 4-hydroxylase subunit alpha

Products: procollagen trans-4-hydroxy-L-proline; succinate; CO2

Alternate protein names: 2OG-Fe(II) Oxygenase; Prolyl 4-Hydroxylase Alpha Subunit; Oxidoreductase 2OG-Fe(II) Oxygenase Family; Oxygenase; Procollagen-Proline 2-Oxoglutarate-4-Dioxygenase; Response Regulator Receiver Domain-Containing Protein; 2OG-Fe(II) Oxygenase Family Oxidoreductase; Prolyl 4-Hydroxylase Subunit Alpha; 2OG-Fe(II) Oxygenase Superfamily Protein; 2OG-Fe(II) Oxygenase Family Protein; Procollagen-Proline 2-Oxoglutarate-4- Dioxygenase; Prolyl 4-Hydroxylase

Number of amino acids: Translated: 232; Mature: 232

Protein sequence:

>232_residues
MIYSCYPIIIFRENREMTNNNQIGENKEQTIFDHKGNVIMTEDREIQIISKFEEPLIVVLGNVLSDEECDELIELSKNKL
ARSKVGSSRDVNDIRTSSGAFLDDNELTEKIEKRISSIMNVPASHGEGLHILNYEVDQQYKAHYDYFAEHSRSAANNRIS
TLVMYLNDVEEGGETFFPKLNLSVHPRKGMAVYFEYFYQDQSLNELTLHGGAPVTKGEKWIATQWVRRGTYK

Sequences:

>Translated_232_residues
MIYSCYPIIIFRENREMTNNNQIGENKEQTIFDHKGNVIMTEDREIQIISKFEEPLIVVLGNVLSDEECDELIELSKNKL
ARSKVGSSRDVNDIRTSSGAFLDDNELTEKIEKRISSIMNVPASHGEGLHILNYEVDQQYKAHYDYFAEHSRSAANNRIS
TLVMYLNDVEEGGETFFPKLNLSVHPRKGMAVYFEYFYQDQSLNELTLHGGAPVTKGEKWIATQWVRRGTYK
>Mature_232_residues
MIYSCYPIIIFRENREMTNNNQIGENKEQTIFDHKGNVIMTEDREIQIISKFEEPLIVVLGNVLSDEECDELIELSKNKL
ARSKVGSSRDVNDIRTSSGAFLDDNELTEKIEKRISSIMNVPASHGEGLHILNYEVDQQYKAHYDYFAEHSRSAANNRIS
TLVMYLNDVEEGGETFFPKLNLSVHPRKGMAVYFEYFYQDQSLNELTLHGGAPVTKGEKWIATQWVRRGTYK

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4758868, Length=186, Percent_Identity=31.7204301075269, Blast_Score=108, Evalue=3e-24,
Organism=Homo sapiens, GI217272863, Length=186, Percent_Identity=31.7204301075269, Blast_Score=108, Evalue=3e-24,
Organism=Homo sapiens, GI63252893, Length=184, Percent_Identity=32.0652173913043, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI63252891, Length=184, Percent_Identity=32.0652173913043, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI217272861, Length=184, Percent_Identity=32.0652173913043, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI63252886, Length=187, Percent_Identity=29.9465240641711, Blast_Score=102, Evalue=2e-22,
Organism=Homo sapiens, GI217272849, Length=187, Percent_Identity=29.9465240641711, Blast_Score=102, Evalue=2e-22,
Organism=Homo sapiens, GI63252888, Length=187, Percent_Identity=29.9465240641711, Blast_Score=102, Evalue=2e-22,
Organism=Homo sapiens, GI33589818, Length=183, Percent_Identity=31.1475409836066, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI217272851, Length=181, Percent_Identity=28.7292817679558, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17552840, Length=189, Percent_Identity=32.2751322751323, Blast_Score=94, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17541712, Length=184, Percent_Identity=29.3478260869565, Blast_Score=94, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI193209070, Length=194, Percent_Identity=27.8350515463918, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI193209068, Length=194, Percent_Identity=27.8350515463918, Blast_Score=75, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI72000637, Length=197, Percent_Identity=26.3959390862944, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24651424, Length=184, Percent_Identity=33.1521739130435, Blast_Score=110, Evalue=9e-25,
Organism=Drosophila melanogaster, GI24651477, Length=183, Percent_Identity=33.3333333333333, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI281362877, Length=226, Percent_Identity=30.5309734513274, Blast_Score=106, Evalue=1e-23,
Organism=Drosophila melanogaster, GI116008432, Length=178, Percent_Identity=32.0224719101124, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI116008128, Length=178, Percent_Identity=32.0224719101124, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI116008434, Length=187, Percent_Identity=28.8770053475936, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI221460681, Length=189, Percent_Identity=31.7460317460317, Blast_Score=103, Evalue=8e-23,
Organism=Drosophila melanogaster, GI281361323, Length=175, Percent_Identity=28.5714285714286, Blast_Score=100, Evalue=7e-22,
Organism=Drosophila melanogaster, GI116008130, Length=181, Percent_Identity=28.7292817679558, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI116008537, Length=181, Percent_Identity=28.7292817679558, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24651407, Length=184, Percent_Identity=27.1739130434783, Blast_Score=98, Evalue=6e-21,
Organism=Drosophila melanogaster, GI78706702, Length=175, Percent_Identity=29.1428571428571, Blast_Score=97, Evalue=8e-21,
Organism=Drosophila melanogaster, GI24651418, Length=185, Percent_Identity=30.2702702702703, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24651420, Length=185, Percent_Identity=28.1081081081081, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24651430, Length=183, Percent_Identity=30.0546448087432, Blast_Score=91, Evalue=8e-19,
Organism=Drosophila melanogaster, GI24651416, Length=190, Percent_Identity=31.5789473684211, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21358309, Length=182, Percent_Identity=32.4175824175824, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21358233, Length=176, Percent_Identity=28.4090909090909, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI221512818, Length=180, Percent_Identity=27.7777777777778, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI45550650, Length=173, Percent_Identity=25.4335260115607, Blast_Score=81, Evalue=7e-16,
Organism=Drosophila melanogaster, GI161076739, Length=177, Percent_Identity=23.1638418079096, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24666354, Length=126, Percent_Identity=27.7777777777778, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 1.14.11.2

Molecular weight: Translated: 26723; Mature: 26723

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYSCYPIIIFRENREMTNNNQIGENKEQTIFDHKGNVIMTEDREIQIISKFEEPLIVVL
CEEEEEEEEEEECCCCCCCCCCCCCCCCCEEEECCCCEEEECCCCEEEEHHCCCCEEEEE
GNVLSDEECDELIELSKNKLARSKVGSSRDVNDIRTSSGAFLDDNELTEKIEKRISSIMN
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCEECCHHHHHHHHHHHHHHHC
VPASHGEGLHILNYEVDQQYKAHYDYFAEHSRSAANNRISTLVMYLNDVEEGGETFFPKL
CCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCEECEEE
NLSVHPRKGMAVYFEYFYQDQSLNELTLHGGAPVTKGEKWIATQWVRRGTYK
CEEECCCCCCEEEEEEHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHCCCCC
>Mature Secondary Structure
MIYSCYPIIIFRENREMTNNNQIGENKEQTIFDHKGNVIMTEDREIQIISKFEEPLIVVL
CEEEEEEEEEEECCCCCCCCCCCCCCCCCEEEECCCCEEEECCCCEEEEHHCCCCEEEEE
GNVLSDEECDELIELSKNKLARSKVGSSRDVNDIRTSSGAFLDDNELTEKIEKRISSIMN
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCEECCHHHHHHHHHHHHHHHC
VPASHGEGLHILNYEVDQQYKAHYDYFAEHSRSAANNRISTLVMYLNDVEEGGETFFPKL
CCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCEECEEE
NLSVHPRKGMAVYFEYFYQDQSLNELTLHGGAPVTKGEKWIATQWVRRGTYK
CEEECCCCCCEEEEEEHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: procollagen L-proline; 2-oxoglutarate; O2

Specific reaction: procollagen L-proline + 2-oxoglutarate + O2 = procollagen trans-4-hydroxy-L-proline + succinate + CO2

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA