Click here to switch to the map view.

The map label for this gene is spoIVB

Identifier: 49480921

GI number: 49480921

Start: 3996624

End: 3997922

Strand: Reverse

Name: spoIVB

Synonym: BT9727_3915

Alternate gene names: 49480921

Gene position: 3997922-3996624 (Counterclockwise)

Preceding gene: 49478569

Following gene: 49481644

Centisome position: 76.33

GC content: 36.41

Gene sequence:

>1299_bases
GTGAACAAATTGAAATTAGAAAGATTTCGCAAGATAATAGGTATTTGTCTCCTTGTTTCTTTAGTTTTTATTGGGTGTTT
TAAACCGCTTCGGACGTTTATTTCATCCCCGAAACAACTTGTTGTTTTTGAAGGACAACAATCAGAAATAGCTTCATTAC
CAGTGTTTAAAGCTTCATCTACAGATCGTCATGTGTTTACAGTAAGTTCAAATGAACAAAAACAAGGACTTATGGTAAAT
TCTCATCAAAATGGTGAAGCGGATATGGTGTTTCAGCTTGCTGGTTTTCCGGTGAAAAAAGTAAATGTGAGAGTATTAAA
AGATTTCAAAGTTATTCCAGGTGGACAATCAATTGGTGTAAAGTTAAACACAAAAGGGGTACTTGTTGTAGGCCATCATT
TAATTCAAACTGAAAAAGGGAAAGTATCTCCTGGTGAAACAGCGGGTGTGCAAATTGGAGATATGATTACTGAAATTAAT
GGTAAAACAATTGAAAGAATGAGTGATGTAGCACCATTTATTCATAATAGTGGGGAAACAGGCGAACCGCTTAATCTCGT
TTTGTTAAGAGATGGAAAGCATATCCGTACTAAGTTAACACCACAAAAAGACAGTGGAGAATCTTCTTATCGCATTGGCT
TATATATTCGTGATTCGGCAGCTGGAATCGGAACAATGACATTTGTTCATCCTGATTCTATGAAATATGGTGCGCTTGGT
CATGTCATTTCTGATAACGATACGAAAAAACCGATTCAAGTCGAAGATGGACAAATTATGCGTTCGACAGTTACATCAAT
TGAAAGAGGTAGTCACGGAAATCCAGGAGAGAAATTAGCGAGGTTCTCACCAGATCGTGAAGTAATTGGCAATATTACAA
TAAATAGTCCATTCGGTATTTTTGGCAAATTGAATACAAATATGACAAATGGCGTAATGGATAAAGCAATGCCTATTGCA
CTATCCCATCAAGTAAAAGAGGGGCCGGCAAAAATATTAACGGTTATTGATCAAGATAAAGTGGAAGCGTTTGATATTGA
AGTTGTTAGTACAGTACCGCAAAAGTTTCCAGCAACAAAAGGCATGGTAATAAAAGTAACAGACAAACGTTTATTAGCGA
AAACGGGTGGTATCGTACAAGGGATGAGCGGAAGTCCAATTGTACAAAATGGGAAAGTAATTGGTGCTGTTACACATGTA
TTTGTAAATGATCCGACATCAGGATATGGTGTTCATATTGAATGGATGTTACATGAAGCTGGAATTAACATTTATGATCA
AGAAAGAAAAGCGAGCTAA

Upstream 100 bases:

>100_bases
AAATGATGATTTCTTAATTCTTCCGGTTATAAATAAAGCATTGCAAGGAGAGATTAAAAAGTGAAGCCAAGAGGCTGAAT
GTGGGGTAGGAGCGAGGAGA

Downstream 100 bases:

>100_bases
TTAATAATTAGCTCGCTTTTCTATTGAAAAATTCGTTGAAATTTTGTAAAAGCTCCGCAAGAGGTGTTTTTTGTTGTAAA
ATAAAAGGAAAGGGTTGAAC

Product: stage IV sporulation protein B

Products: NA

Alternate protein names: Sporulation factor IV B protease; Stage IV sporulation protein B [H]

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MNKLKLERFRKIIGICLLVSLVFIGCFKPLRTFISSPKQLVVFEGQQSEIASLPVFKASSTDRHVFTVSSNEQKQGLMVN
SHQNGEADMVFQLAGFPVKKVNVRVLKDFKVIPGGQSIGVKLNTKGVLVVGHHLIQTEKGKVSPGETAGVQIGDMITEIN
GKTIERMSDVAPFIHNSGETGEPLNLVLLRDGKHIRTKLTPQKDSGESSYRIGLYIRDSAAGIGTMTFVHPDSMKYGALG
HVISDNDTKKPIQVEDGQIMRSTVTSIERGSHGNPGEKLARFSPDREVIGNITINSPFGIFGKLNTNMTNGVMDKAMPIA
LSHQVKEGPAKILTVIDQDKVEAFDIEVVSTVPQKFPATKGMVIKVTDKRLLAKTGGIVQGMSGSPIVQNGKVIGAVTHV
FVNDPTSGYGVHIEWMLHEAGINIYDQERKAS

Sequences:

>Translated_432_residues
MNKLKLERFRKIIGICLLVSLVFIGCFKPLRTFISSPKQLVVFEGQQSEIASLPVFKASSTDRHVFTVSSNEQKQGLMVN
SHQNGEADMVFQLAGFPVKKVNVRVLKDFKVIPGGQSIGVKLNTKGVLVVGHHLIQTEKGKVSPGETAGVQIGDMITEIN
GKTIERMSDVAPFIHNSGETGEPLNLVLLRDGKHIRTKLTPQKDSGESSYRIGLYIRDSAAGIGTMTFVHPDSMKYGALG
HVISDNDTKKPIQVEDGQIMRSTVTSIERGSHGNPGEKLARFSPDREVIGNITINSPFGIFGKLNTNMTNGVMDKAMPIA
LSHQVKEGPAKILTVIDQDKVEAFDIEVVSTVPQKFPATKGMVIKVTDKRLLAKTGGIVQGMSGSPIVQNGKVIGAVTHV
FVNDPTSGYGVHIEWMLHEAGINIYDQERKAS
>Mature_432_residues
MNKLKLERFRKIIGICLLVSLVFIGCFKPLRTFISSPKQLVVFEGQQSEIASLPVFKASSTDRHVFTVSSNEQKQGLMVN
SHQNGEADMVFQLAGFPVKKVNVRVLKDFKVIPGGQSIGVKLNTKGVLVVGHHLIQTEKGKVSPGETAGVQIGDMITEIN
GKTIERMSDVAPFIHNSGETGEPLNLVLLRDGKHIRTKLTPQKDSGESSYRIGLYIRDSAAGIGTMTFVHPDSMKYGALG
HVISDNDTKKPIQVEDGQIMRSTVTSIERGSHGNPGEKLARFSPDREVIGNITINSPFGIFGKLNTNMTNGVMDKAMPIA
LSHQVKEGPAKILTVIDQDKVEAFDIEVVSTVPQKFPATKGMVIKVTDKRLLAKTGGIVQGMSGSPIVQNGKVIGAVTHV
FVNDPTSGYGVHIEWMLHEAGINIYDQERKAS

Specific function: Plays a central role in the sigma-K checkpoint which coordinates gene expression during the later stages of spore formation. The protease is activated by trans cleavage of the zymogen precursor producing SpoIVB-45 kDa. This undergoes further trimming by c

COG id: COG0750

COG function: function code M; Predicted membrane-associated Zn-dependent proteases 1

Gene ontology:

Cell location: Forespore intermembrane space [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 peptidase S55 domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001478
- InterPro:   IPR014219
- InterPro:   IPR008763 [H]

Pfam domain/function: PF00595 PDZ; PF05580 Peptidase_S55 [H]

EC number: =3.4.21.116 [H]

Molecular weight: Translated: 46975; Mature: 46975

Theoretical pI: Translated: 9.92; Mature: 9.92

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKLKLERFRKIIGICLLVSLVFIGCFKPLRTFISSPKQLVVFEGQQSEIASLPVFKASS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHCCCEEECCC
TDRHVFTVSSNEQKQGLMVNSHQNGEADMVFQLAGFPVKKVNVRVLKDFKVIPGGQSIGV
CCCEEEEECCCCCCCCEEEECCCCCCCEEEEEECCCCEEEEEEEEEEEEEECCCCCEEEE
KLNTKGVLVVGHHLIQTEKGKVSPGETAGVQIGDMITEINGKTIERMSDVAPFIHNSGET
EECCCEEEEEEEEEEECCCCCCCCCCCCCEEHHHHHHHCCCHHHHHHHHHHHHHCCCCCC
GEPLNLVLLRDGKHIRTKLTPQKDSGESSYRIGLYIRDSAAGIGTMTFVHPDSMKYGALG
CCCEEEEEEECCCEEEEECCCCCCCCCCCEEEEEEEECCCCCCEEEEEECCCCCCCCCCC
HVISDNDTKKPIQVEDGQIMRSTVTSIERGSHGNPGEKLARFSPDREVIGNITINSPFGI
EEECCCCCCCCEEECCCCHHHHHHHHHHCCCCCCCHHHHHHCCCCCCEEEEEEECCCCCE
FGKLNTNMTNGVMDKAMPIALSHQVKEGPAKILTVIDQDKVEAFDIEVVSTVPQKFPATK
EEEECCCCCCCHHHHCCCEEEHHHCCCCCEEEEEEECCCCCEEEEEEHHHHCHHHCCCCC
GMVIKVTDKRLLAKTGGIVQGMSGSPIVQNGKVIGAVTHVFVNDPTSGYGVHIEWMLHEA
CEEEEEECCCHHHHCCCEEECCCCCCEEECCEEEEEEEEEEECCCCCCCEEEEEEEEEEC
GINIYDQERKAS
CCCEECCCCCCC
>Mature Secondary Structure
MNKLKLERFRKIIGICLLVSLVFIGCFKPLRTFISSPKQLVVFEGQQSEIASLPVFKASS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHCCCEEECCC
TDRHVFTVSSNEQKQGLMVNSHQNGEADMVFQLAGFPVKKVNVRVLKDFKVIPGGQSIGV
CCCEEEEECCCCCCCCEEEECCCCCCCEEEEEECCCCEEEEEEEEEEEEEECCCCCEEEE
KLNTKGVLVVGHHLIQTEKGKVSPGETAGVQIGDMITEINGKTIERMSDVAPFIHNSGET
EECCCEEEEEEEEEEECCCCCCCCCCCCCEEHHHHHHHCCCHHHHHHHHHHHHHCCCCCC
GEPLNLVLLRDGKHIRTKLTPQKDSGESSYRIGLYIRDSAAGIGTMTFVHPDSMKYGALG
CCCEEEEEEECCCEEEEECCCCCCCCCCCEEEEEEEECCCCCCEEEEEECCCCCCCCCCC
HVISDNDTKKPIQVEDGQIMRSTVTSIERGSHGNPGEKLARFSPDREVIGNITINSPFGI
EEECCCCCCCCEEECCCCHHHHHHHHHHCCCCCCCHHHHHHCCCCCCEEEEEEECCCCCE
FGKLNTNMTNGVMDKAMPIALSHQVKEGPAKILTVIDQDKVEAFDIEVVSTVPQKFPATK
EEEECCCCCCCHHHHCCCEEEHHHCCCCCEEEEEEECCCCCEEEEEEHHHHCHHHCCCCC
GMVIKVTDKRLLAKTGGIVQGMSGSPIVQNGKVIGAVTHVFVNDPTSGYGVHIEWMLHEA
CEEEEEECCCHHHHCCCEEECCCCCCEEECCEEEEEEEEEEECCCCCCCEEEEEEEEEEC
GINIYDQERKAS
CCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2106508; 8969508; 9384377; 11741860 [H]