Click here to switch to the map view.

The map label for this gene is recN

Identifier: 49478569

GI number: 49478569

Start: 3998041

End: 3999780

Strand: Reverse

Name: recN

Synonym: BT9727_3916

Alternate gene names: 49478569

Gene position: 3999780-3998041 (Counterclockwise)

Preceding gene: 49478570

Following gene: 49480921

Centisome position: 76.37

GC content: 35.92

Gene sequence:

>1740_bases
TTGTTATCGGAATTATCGATTAGAAACTTTGCTATTATTGAGGCATTAAATATTTCTTTTCAAAAAGGATTAACGGTTTT
AAGTGGTGAAACAGGGGCCGGAAAATCGATTATTATTGATGCGATTAGTTTACTTGTAGGTGGCCGCGGTTCGGCAGAAT
TTGTTCGATACGGAACAGAAAAAGCTGAGATAGAGGGGCTATTTTATGTGGAAGATGATAAGCATCCATGTATTGAAAAG
GCAGAAGAATTGGATATAGAAATAGAAGACGGCATGATTATTTTGAAGCGTGATATCGCTGCAAACGGAAAAAGCGTATG
TCGTGTAAATGGAAAACTAGTTACCCTTAGTGTATTAAAAGAAATTGGAAAAACACTTGTTGATATTCATGGGCAACACG
AAACGCAAGATTTAATGAATGAAGAACGTCATCTATTTATGCTCGATCATTTTGATGGGGAGCGTATCGTAAAACAATTG
GATATATACCAAAACGTATACGCTGATTATGAGAAGTTAAAAAAACAATTGAAATCGTTAAGTGAAAATGAACAACAAAT
GGCGCATCGCTTAGATTTAATTCAATTCCAGCATGAAGAAATCCGTAAGGCGGATTTAAAGATTGATGAAGAAAATAACT
TAACTGAAGAACGATTGCAAATTGCTAATTTCGAAAAGATTTATAAAGCATTAGGTGACGCATATCGCTCGTTAAGCGCT
GATGGACAAGGGTTAGATAATGTAAGAAGTGCAATGGGACAAATGGAGAGTATTACACATTTAGACGAAGTGTACCAAGA
AAATCATGATTCAATTGCAAATAGCTATTACCTATTAGAAGAAGTTGCATATCAACTAAGAGAAAAACTCGATATGATGG
AATATGACCCAAATCGTTTAGATGAAATTGAAACGCGTTTAAATGAAATTCGTATGCTAAAGAGAAAATATGGAAATACT
GTAGAAGAGATATTAGCGTATGCTGATAAAATTGAACAAGAGATTTTTACGATTGAAAATAAAGACGTTCATATTGAAAC
GACGAAGAAACAGTTGAAGGAATTAGAAAGTGTTATTCTGAAAGAAGCAACGTTGTTAAGTAATATGCGTCATGAACTTG
CAGAGCATCTTACAAATGCCATTCATCAGGAATTAAAAGAATTGTATATGGAAAAAACAAAATTTGAAGTGAGAATCATA
AAGCGAGAAGGAAATGCGGAAGAGCCTCTCGTGGAGGGAGCACCGGTAAGGCTTACAGCGGAAGGTTATGATCATGTGGA
ATTTTATATTTCAACCAATCCAGGTGAGCCGCTAAAACCACTTTCAAAGGTCGCTTCTGGCGGAGAGTTGTCCCGTATTA
TTTTAGCTTTAAAAAGTATTTTTTCTAAGCATCAAGGTGTTGCATCTGTTATTTTTGATGAAGTGGATACTGGTGTAAGT
GGCCGGGTCGCACAGGCTATTGCGGAAAAAATTTATCGAGTATCAGTAAACTCACAAGTACTTTGTATTACGCACTTACC
TCAAGTAGCTTCAATGGCGGATTCGCATTTATTTATCCGAAAACAAGTAGCGAATGATCGAACAATTACATCCGTTACCG
TTTTAACTATGGAGGATAAAGTAACAGAAATAGCTCGAATGATTTCTGGTGTGGAAATTACAGATTTAACGACAGAACAT
GCGAAAGAATTACTTACGCAAGCGCATCATTTTAAACAGACAGCAGAGGCTATCCAGTAA

Upstream 100 bases:

>100_bases
AAAGTAATTTATACATACAAACATATAGTATGACTATTTGAATAAAGAACTGAGAAAAGCCCTTAATAGGGGATTAGTTT
ATAACGAGGTGAATGGGGCA

Downstream 100 bases:

>100_bases
TGGATAGCTTTTTTTCTCAAATGATGATTTCTTAATTCTTCCGGTTATAAATAAAGCATTGCAAGGAGAGATTAAAAAGT
GAAGCCAAGAGGCTGAATGT

Product: DNA repair protein

Products: NA

Alternate protein names: Recombination protein N [H]

Number of amino acids: Translated: 579; Mature: 579

Protein sequence:

>579_residues
MLSELSIRNFAIIEALNISFQKGLTVLSGETGAGKSIIIDAISLLVGGRGSAEFVRYGTEKAEIEGLFYVEDDKHPCIEK
AEELDIEIEDGMIILKRDIAANGKSVCRVNGKLVTLSVLKEIGKTLVDIHGQHETQDLMNEERHLFMLDHFDGERIVKQL
DIYQNVYADYEKLKKQLKSLSENEQQMAHRLDLIQFQHEEIRKADLKIDEENNLTEERLQIANFEKIYKALGDAYRSLSA
DGQGLDNVRSAMGQMESITHLDEVYQENHDSIANSYYLLEEVAYQLREKLDMMEYDPNRLDEIETRLNEIRMLKRKYGNT
VEEILAYADKIEQEIFTIENKDVHIETTKKQLKELESVILKEATLLSNMRHELAEHLTNAIHQELKELYMEKTKFEVRII
KREGNAEEPLVEGAPVRLTAEGYDHVEFYISTNPGEPLKPLSKVASGGELSRIILALKSIFSKHQGVASVIFDEVDTGVS
GRVAQAIAEKIYRVSVNSQVLCITHLPQVASMADSHLFIRKQVANDRTITSVTVLTMEDKVTEIARMISGVEITDLTTEH
AKELLTQAHHFKQTAEAIQ

Sequences:

>Translated_579_residues
MLSELSIRNFAIIEALNISFQKGLTVLSGETGAGKSIIIDAISLLVGGRGSAEFVRYGTEKAEIEGLFYVEDDKHPCIEK
AEELDIEIEDGMIILKRDIAANGKSVCRVNGKLVTLSVLKEIGKTLVDIHGQHETQDLMNEERHLFMLDHFDGERIVKQL
DIYQNVYADYEKLKKQLKSLSENEQQMAHRLDLIQFQHEEIRKADLKIDEENNLTEERLQIANFEKIYKALGDAYRSLSA
DGQGLDNVRSAMGQMESITHLDEVYQENHDSIANSYYLLEEVAYQLREKLDMMEYDPNRLDEIETRLNEIRMLKRKYGNT
VEEILAYADKIEQEIFTIENKDVHIETTKKQLKELESVILKEATLLSNMRHELAEHLTNAIHQELKELYMEKTKFEVRII
KREGNAEEPLVEGAPVRLTAEGYDHVEFYISTNPGEPLKPLSKVASGGELSRIILALKSIFSKHQGVASVIFDEVDTGVS
GRVAQAIAEKIYRVSVNSQVLCITHLPQVASMADSHLFIRKQVANDRTITSVTVLTMEDKVTEIARMISGVEITDLTTEH
AKELLTQAHHFKQTAEAIQ
>Mature_579_residues
MLSELSIRNFAIIEALNISFQKGLTVLSGETGAGKSIIIDAISLLVGGRGSAEFVRYGTEKAEIEGLFYVEDDKHPCIEK
AEELDIEIEDGMIILKRDIAANGKSVCRVNGKLVTLSVLKEIGKTLVDIHGQHETQDLMNEERHLFMLDHFDGERIVKQL
DIYQNVYADYEKLKKQLKSLSENEQQMAHRLDLIQFQHEEIRKADLKIDEENNLTEERLQIANFEKIYKALGDAYRSLSA
DGQGLDNVRSAMGQMESITHLDEVYQENHDSIANSYYLLEEVAYQLREKLDMMEYDPNRLDEIETRLNEIRMLKRKYGNT
VEEILAYADKIEQEIFTIENKDVHIETTKKQLKELESVILKEATLLSNMRHELAEHLTNAIHQELKELYMEKTKFEVRII
KREGNAEEPLVEGAPVRLTAEGYDHVEFYISTNPGEPLKPLSKVASGGELSRIILALKSIFSKHQGVASVIFDEVDTGVS
GRVAQAIAEKIYRVSVNSQVLCITHLPQVASMADSHLFIRKQVANDRTITSVTVLTMEDKVTEIARMISGVEITDLTTEH
AKELLTQAHHFKQTAEAIQ

Specific function: Involved in recombinational repair of damaged DNA. Seems to be the first protein recruited to repair centers, foci that are the site of double-strand DNA break(s), followed by recO and then recF [H]

COG id: COG0497

COG function: function code L; ATPase involved in DNA repair

Gene ontology:

Cell location: Cytoplasm, nucleoid. Note=Cytoplasmically located in untreated cells. Recruited to foci following treatment with DNA damaging agents; these foci are presumably the breaks themselves. They are almost always located within nucleoids [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recN family [H]

Homologues:

Organism=Escherichia coli, GI48994901, Length=573, Percent_Identity=32.8097731239092, Blast_Score=294, Evalue=9e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004604
- InterPro:   IPR003395 [H]

Pfam domain/function: PF02463 SMC_N [H]

EC number: NA

Molecular weight: Translated: 65756; Mature: 65756

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSELSIRNFAIIEALNISFQKGLTVLSGETGAGKSIIIDAISLLVGGRGSAEFVRYGTE
CCCCCCCCHHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHCCCCCHHHHHCCCC
KAEIEGLFYVEDDKHPCIEKAEELDIEIEDGMIILKRDIAANGKSVCRVNGKLVTLSVLK
HHHCCEEEEEECCCCCHHHHHHHCCEEEECCEEEEEECCCCCCCCEEEECCEEEHHHHHH
EIGKTLVDIHGQHETQDLMNEERHLFMLDHFDGERIVKQLDIYQNVYADYEKLKKQLKSL
HHHHHHHHHCCCCHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SENEQQMAHRLDLIQFQHEEIRKADLKIDEENNLTEERLQIANFEKIYKALGDAYRSLSA
CCHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
DGQGLDNVRSAMGQMESITHLDEVYQENHDSIANSYYLLEEVAYQLREKLDMMEYDPNRL
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
DEIETRLNEIRMLKRKYGNTVEEILAYADKIEQEIFTIENKDVHIETTKKQLKELESVIL
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEECCCCEEEEHHHHHHHHHHHHHH
KEATLLSNMRHELAEHLTNAIHQELKELYMEKTKFEVRIIKREGNAEEPLVEGAPVRLTA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCCCCEEEEE
EGYDHVEFYISTNPGEPLKPLSKVASGGELSRIILALKSIFSKHQGVASVIFDEVDTGVS
CCCCEEEEEEECCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCC
GRVAQAIAEKIYRVSVNSQVLCITHLPQVASMADSHLFIRKQVANDRTITSVTVLTMEDK
HHHHHHHHHHHHHEECCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEHHH
VTEIARMISGVEITDLTTEHAKELLTQAHHFKQTAEAIQ
HHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLSELSIRNFAIIEALNISFQKGLTVLSGETGAGKSIIIDAISLLVGGRGSAEFVRYGTE
CCCCCCCCHHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHCCCCCHHHHHCCCC
KAEIEGLFYVEDDKHPCIEKAEELDIEIEDGMIILKRDIAANGKSVCRVNGKLVTLSVLK
HHHCCEEEEEECCCCCHHHHHHHCCEEEECCEEEEEECCCCCCCCEEEECCEEEHHHHHH
EIGKTLVDIHGQHETQDLMNEERHLFMLDHFDGERIVKQLDIYQNVYADYEKLKKQLKSL
HHHHHHHHHCCCCHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SENEQQMAHRLDLIQFQHEEIRKADLKIDEENNLTEERLQIANFEKIYKALGDAYRSLSA
CCHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
DGQGLDNVRSAMGQMESITHLDEVYQENHDSIANSYYLLEEVAYQLREKLDMMEYDPNRL
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
DEIETRLNEIRMLKRKYGNTVEEILAYADKIEQEIFTIENKDVHIETTKKQLKELESVIL
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEECCCCEEEEHHHHHHHHHHHHHH
KEATLLSNMRHELAEHLTNAIHQELKELYMEKTKFEVRIIKREGNAEEPLVEGAPVRLTA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCCCCEEEEE
EGYDHVEFYISTNPGEPLKPLSKVASGGELSRIILALKSIFSKHQGVASVIFDEVDTGVS
CCCCEEEEEEECCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCC
GRVAQAIAEKIYRVSVNSQVLCITHLPQVASMADSHLFIRKQVANDRTITSVTVLTMEDK
HHHHHHHHHHHHHEECCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEHHH
VTEIARMISGVEITDLTTEHAKELLTQAHHFKQTAEAIQ
HHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2106508; 8969508; 9384377; 2507400; 9044256 [H]