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The map label for this gene is ndhF

Identifier: 49479131

GI number: 49479131

Start: 3015188

End: 3016720

Strand: Reverse

Name: ndhF

Synonym: BT9727_2938

Alternate gene names: 49479131

Gene position: 3016720-3015188 (Counterclockwise)

Preceding gene: 49479520

Following gene: 49479522

Centisome position: 57.6

GC content: 36.59

Gene sequence:

>1533_bases
ATGTTAATTTCGCTAAGTTCATCAACACTGTTAACATTATTTTTTATCGCGCTTAGTGCTTCTTGGCTAAGTGGATTATT
GTTTTTACATGCAAGGATGCCTTTACGTTTTGTTCATATCCATATTGGTATCGCTGCTTTGCCTTCATTGGTTTCTTTAC
TTGCACTTGTTAATAACAATGGAGATAGAGTTGTGGGGCCTTGGCATCTAGATACCTTAGCTTGGTTAATGGCTTTCTTC
GTTCTTACAATTGGTTTAATCATTCAGCGATTTTCTGTACGTTACTTAATGGGAGATCGCTCATATCGAAAATACTTTGC
ACTTTTTACATTTACTACAGGTGTATCTTCAGTTGCATGGTTAAGTGACGATCTTCGCTTCATGATTATGTGTTGGGGTG
CAACACTTATAGGGTTGGTTCTACTCATCGGGCTAAATAAAGGGTGGAAGGTAGTTAGTGAAGCAACAAAAATCTCAGGC
TATTTATTTACAATAAGTTGGATTGCCTTACTGTTAGCTATCATTTGGCTGTTTCAAATAACTGGACAGTGGCAGGTAAC
ATCAGTTTTAACGAATGAAAATGTAGCCCAATTTGGAACATTGGAGAAAACAGGAATTAACTTATTGATTATAGTAGCTG
TGATGATTCCAGCAGCACAATGGCCTTTTCAAAGATGGTTAATTGAATCAGCTGTTGCTCCAACTCCTGTTTCTGCTATT
ATGCATGCGGGTTTAGTAAATGCTGGCGGTATCATGTTAACTAGATTTTCGCCACTTTTTCATGATGATATTGCACAAAT
CATTTTACTAATTTTCTCTAGTATTTCTGTCTTAATAGGAACAGGAATTAGTTTAGTTCAAGTTGATTATAAACGTCAGC
TAGTAGGATCTACTATTGCACAAATGGGATTTATGCTGATTCAATGTGCATTAGGCGCTTATTTAGCAGCTGTTATTCAT
TTAATTTTACATGGTTTATTTAAAGCCACACTGTTTTTACAAGCTGGTTCCTCAGTACAACGTGTTGAGGTAGTGAAGCA
GTCTAATAAAAAGATGTCCAATTTATGGATGATAGTCGGGCGTGTTTTAGGGTTATTTATAGCAATTGCTTTCTGGTTTA
TAACTTCTGGAGAGGGGTATCAATTAGTTAGTGCGTTAATCTTAGGCTGGTCACTATACTTTTCTTGGAAACAGCTTGTT
GTTTTTGGAGAGGGAAGAATGGGACGAATTGCTGGCTTAATTGTTTTGATTGGATTTTCTCTCATTTACTTTACCGTTCA
TAATTCTCTTTATAAATGGTTGCACACTGACATGTATCAAAGTGTTCAACCTTCAGCTCCAGCCGTTATCTTTGTTATAT
GTACCTTACTATTTAGTAGTGTTATTTGTACTTTCGTTACTCGTAATCAATCTTCTACTTTATCCGCTGTACTTTATCTT
TGGTCAGTTCGAGTAGGTGAGGCGAGACGAAAGTCAGTAGAAAGTCATCCAAGCTATCTGAAACATGATGTATCAAAGGG
AGGTAATTCGTGA

Upstream 100 bases:

>100_bases
ACGATGTACTAAAAAGAGTGAATAAAAATTGAAATTAAAAAACTATCGTAATGAAAAGGAATTTTTATAGGTTATTTTCG
TTTGGAAGGGAGTATTTTAC

Downstream 100 bases:

>100_bases
TGAGCATACCGTCAATATTAAGGAAAGAGACTTTAAAAAAGAAAGATAAAAATATTGATCTACAAGAAAATAATATAAAT
GATTTAGTTGTATCGGCTAG

Product: NADH dehydrogenase subunit 5

Products: NA

Alternate protein names: NADH dehydrogenase subunit 5 [H]

Number of amino acids: Translated: 510; Mature: 510

Protein sequence:

>510_residues
MLISLSSSTLLTLFFIALSASWLSGLLFLHARMPLRFVHIHIGIAALPSLVSLLALVNNNGDRVVGPWHLDTLAWLMAFF
VLTIGLIIQRFSVRYLMGDRSYRKYFALFTFTTGVSSVAWLSDDLRFMIMCWGATLIGLVLLIGLNKGWKVVSEATKISG
YLFTISWIALLLAIIWLFQITGQWQVTSVLTNENVAQFGTLEKTGINLLIIVAVMIPAAQWPFQRWLIESAVAPTPVSAI
MHAGLVNAGGIMLTRFSPLFHDDIAQIILLIFSSISVLIGTGISLVQVDYKRQLVGSTIAQMGFMLIQCALGAYLAAVIH
LILHGLFKATLFLQAGSSVQRVEVVKQSNKKMSNLWMIVGRVLGLFIAIAFWFITSGEGYQLVSALILGWSLYFSWKQLV
VFGEGRMGRIAGLIVLIGFSLIYFTVHNSLYKWLHTDMYQSVQPSAPAVIFVICTLLFSSVICTFVTRNQSSTLSAVLYL
WSVRVGEARRKSVESHPSYLKHDVSKGGNS

Sequences:

>Translated_510_residues
MLISLSSSTLLTLFFIALSASWLSGLLFLHARMPLRFVHIHIGIAALPSLVSLLALVNNNGDRVVGPWHLDTLAWLMAFF
VLTIGLIIQRFSVRYLMGDRSYRKYFALFTFTTGVSSVAWLSDDLRFMIMCWGATLIGLVLLIGLNKGWKVVSEATKISG
YLFTISWIALLLAIIWLFQITGQWQVTSVLTNENVAQFGTLEKTGINLLIIVAVMIPAAQWPFQRWLIESAVAPTPVSAI
MHAGLVNAGGIMLTRFSPLFHDDIAQIILLIFSSISVLIGTGISLVQVDYKRQLVGSTIAQMGFMLIQCALGAYLAAVIH
LILHGLFKATLFLQAGSSVQRVEVVKQSNKKMSNLWMIVGRVLGLFIAIAFWFITSGEGYQLVSALILGWSLYFSWKQLV
VFGEGRMGRIAGLIVLIGFSLIYFTVHNSLYKWLHTDMYQSVQPSAPAVIFVICTLLFSSVICTFVTRNQSSTLSAVLYL
WSVRVGEARRKSVESHPSYLKHDVSKGGNS
>Mature_510_residues
MLISLSSSTLLTLFFIALSASWLSGLLFLHARMPLRFVHIHIGIAALPSLVSLLALVNNNGDRVVGPWHLDTLAWLMAFF
VLTIGLIIQRFSVRYLMGDRSYRKYFALFTFTTGVSSVAWLSDDLRFMIMCWGATLIGLVLLIGLNKGWKVVSEATKISG
YLFTISWIALLLAIIWLFQITGQWQVTSVLTNENVAQFGTLEKTGINLLIIVAVMIPAAQWPFQRWLIESAVAPTPVSAI
MHAGLVNAGGIMLTRFSPLFHDDIAQIILLIFSSISVLIGTGISLVQVDYKRQLVGSTIAQMGFMLIQCALGAYLAAVIH
LILHGLFKATLFLQAGSSVQRVEVVKQSNKKMSNLWMIVGRVLGLFIAIAFWFITSGEGYQLVSALILGWSLYFSWKQLV
VFGEGRMGRIAGLIVLIGFSLIYFTVHNSLYKWLHTDMYQSVQPSAPAVIFVICTLLFSSVICTFVTRNQSSTLSAVLYL
WSVRVGEARRKSVESHPSYLKHDVSKGGNS

Specific function: Ndh-1 Shuttles Electrons From NADH, Via Fmn And Iron- Sulfur (Fe-S) Centers, To Quinones In The Respiratory Chain. The Immediate Electron Acceptor For The Enzyme In This Species Is Believed To Be Ubiquinone. Couples The Redox Reaction To Proton Translocat

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=140, Percent_Identity=32.8571428571429, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1788614, Length=412, Percent_Identity=26.9417475728155, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1788613, Length=271, Percent_Identity=28.4132841328413, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788831, Length=125, Percent_Identity=33.6, Blast_Score=63, Evalue=4e-11,
Organism=Escherichia coli, GI1788829, Length=304, Percent_Identity=26.6447368421053, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR001516 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF00662 Oxidored_q1_N [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 56514; Mature: 56514

Theoretical pI: Translated: 10.18; Mature: 10.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLISLSSSTLLTLFFIALSASWLSGLLFLHARMPLRFVHIHIGIAALPSLVSLLALVNNN
CEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHCCC
GDRVVGPWHLDTLAWLMAFFVLTIGLIIQRFSVRYLMGDRSYRKYFALFTFTTGVSSVAW
CCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHHHH
LSDDLRFMIMCWGATLIGLVLLIGLNKGWKVVSEATKISGYLFTISWIALLLAIIWLFQI
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TGQWQVTSVLTNENVAQFGTLEKTGINLLIIVAVMIPAAQWPFQRWLIESAVAPTPVSAI
CCCEEEEEEECCCCHHHHCCCHHCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHH
MHAGLVNAGGIMLTRFSPLFHDDIAQIILLIFSSISVLIGTGISLVQVDYKRQLVGSTIA
HHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHH
QMGFMLIQCALGAYLAAVIHLILHGLFKATLFLQAGSSVQRVEVVKQSNKKMSNLWMIVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCHHHHHHHHHHH
RVLGLFIAIAFWFITSGEGYQLVSALILGWSLYFSWKQLVVFGEGRMGRIAGLIVLIGFS
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHH
LIYFTVHNSLYKWLHTDMYQSVQPSAPAVIFVICTLLFSSVICTFVTRNQSSTLSAVLYL
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
WSVRVGEARRKSVESHPSYLKHDVSKGGNS
HHHHHCHHHHHHHHCCHHHHHHHHHCCCCC
>Mature Secondary Structure
MLISLSSSTLLTLFFIALSASWLSGLLFLHARMPLRFVHIHIGIAALPSLVSLLALVNNN
CEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHCCC
GDRVVGPWHLDTLAWLMAFFVLTIGLIIQRFSVRYLMGDRSYRKYFALFTFTTGVSSVAW
CCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHHHH
LSDDLRFMIMCWGATLIGLVLLIGLNKGWKVVSEATKISGYLFTISWIALLLAIIWLFQI
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TGQWQVTSVLTNENVAQFGTLEKTGINLLIIVAVMIPAAQWPFQRWLIESAVAPTPVSAI
CCCEEEEEEECCCCHHHHCCCHHCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHH
MHAGLVNAGGIMLTRFSPLFHDDIAQIILLIFSSISVLIGTGISLVQVDYKRQLVGSTIA
HHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHH
QMGFMLIQCALGAYLAAVIHLILHGLFKATLFLQAGSSVQRVEVVKQSNKKMSNLWMIVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCHHHHHHHHHHH
RVLGLFIAIAFWFITSGEGYQLVSALILGWSLYFSWKQLVVFGEGRMGRIAGLIVLIGFS
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHH
LIYFTVHNSLYKWLHTDMYQSVQPSAPAVIFVICTLLFSSVICTFVTRNQSSTLSAVLYL
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
WSVRVGEARRKSVESHPSYLKHDVSKGGNS
HHHHHCHHHHHHHHCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]