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The map label for this gene is gidA

Identifier: 49478916

GI number: 49478916

Start: 3668621

End: 3669925

Strand: Reverse

Name: gidA

Synonym: BT9727_3573

Alternate gene names: 49478916

Gene position: 3669925-3668621 (Counterclockwise)

Preceding gene: 49478403

Following gene: 49478402

Centisome position: 70.07

GC content: 39.69

Gene sequence:

>1305_bases
ATGACAACACAAGTAGTAAACGTCATTGGCGCAGGTCTTGCAGGAAGTGAAGCAGCTTATCAAATTGCAAAACGTGGTGT
CCAAGTAAGATTATACGAAATGAGACCGGTAAGGCAAACGCCAGCTCATCACACAGATAAATTTGCAGAATTAGTATGTA
GTAACTCACTTCGCGCAAACACATTAACAAATGCTGTTGGTGTTATTAAAGAAGAAATGCGTTTAATGGATTCTGTTATT
ATTCGTGCAGCTGATGAGTGTTCTGTACCAGCAGGAGGAGCTTTAGCGGTAGATCGTCATGAATTTGCGGCAAAAGTAAC
GGAATATGTGAAAAACCATCCGAACGTAACTGTGATGAATGAAGAAATCACTGAAATTCCAGAAGGTCCTACTATTATCG
CGACAGGTCCACTTACGTCACCGGATCTTTCTGCACAATTAAAAGAATTAACGGGTGAAGACTACTTTTATTTCTATGAT
GCTGCAGCACCGATTGTTGAGAAAGACAGCATTGATATGAATAAGGTGTATTTAAAATCTCGTTACGATAAAGGTGAAGC
GGCATATTTAAACTGCCCAATGACGGAAGAGGAATTTGACCGTTTTTACGAGGCTTTAATCGCTGCTGAAACAGTACCTT
TAAAAGAATTTGAAAAAGAAATTTTCTTTGAAGGTTGTATGCCAGTAGAAGTTATGGCAAGTAGAGGAAGACAAACACTA
GTATTTGGACCGATGAAACCTGTTGGATTAGAAGATCCGAAAACAGGGAAAACGCCATATGCTGTTGTTCAGTTACGTCA
AGATGATGCAGCGGGAACGTTATACAATATCGTAGGTTTCCAAACGCATTTAAAATGGGGACCACAAAAAGAAGTGTTAC
AGTTGATTCCAGGACTGGAAAACGCAGAGATTGTACGTTATGGTGTAATGCATCGTAATACGTTTATTAATTCACCTAAC
TTGCTTCGTCCAACATACCAATATAAACAACGTGATGATTTATTCTTCGCTGGTCAAATGACTGGAGTAGAGGGATATGT
TGAATCGGCAGCTTCAGGATTGTTAGCAGGTATCAACGCTGCTCGACTTGTAAAAGGTGAAGAGCCGGTTGTATTACCAC
CTGTAACTGCAATGGGAAGTATGGCGAATTATATTACTGCGACAAACGCGAAAAACTTCCAACCAATGAATGCGAACTTC
GGTCTGTTTGCACCGTTAGAGAAGAAAATTAAGAAGAAAGCAGAACGTAATGAAGCCTATGCAACACGCGCTTTAGAAAC
GATTCGAAATTTTGTAAATATTTAG

Upstream 100 bases:

>100_bases
TACAAGTACAATGTATTTCGTGTGATTATGAAGAAGAACAACAAATGTGAGCGTAACTGCTCACATTTTTTTCGGGAAGA
AAAGGAAAGGTGATAAATAT

Downstream 100 bases:

>100_bases
TTAAATTTCTTGCAAAGGCTACTAAGTTGTGATACGATTTAGTAGCCTTTATTGAGGTGAATTGTGTGAATGTGAAGAAA
TTGTTACAATTATTCGTTGG

Product: tRNA (uracil-5-)-methyltransferase Gid

Products: NA

Alternate protein names: Folate-dependent tRNA (uracil-5-)-methyltransferase; Folate-dependent tRNA(M-5-U54)-methyltransferase [H]

Number of amino acids: Translated: 434; Mature: 433

Protein sequence:

>434_residues
MTTQVVNVIGAGLAGSEAAYQIAKRGVQVRLYEMRPVRQTPAHHTDKFAELVCSNSLRANTLTNAVGVIKEEMRLMDSVI
IRAADECSVPAGGALAVDRHEFAAKVTEYVKNHPNVTVMNEEITEIPEGPTIIATGPLTSPDLSAQLKELTGEDYFYFYD
AAAPIVEKDSIDMNKVYLKSRYDKGEAAYLNCPMTEEEFDRFYEALIAAETVPLKEFEKEIFFEGCMPVEVMASRGRQTL
VFGPMKPVGLEDPKTGKTPYAVVQLRQDDAAGTLYNIVGFQTHLKWGPQKEVLQLIPGLENAEIVRYGVMHRNTFINSPN
LLRPTYQYKQRDDLFFAGQMTGVEGYVESAASGLLAGINAARLVKGEEPVVLPPVTAMGSMANYITATNAKNFQPMNANF
GLFAPLEKKIKKKAERNEAYATRALETIRNFVNI

Sequences:

>Translated_434_residues
MTTQVVNVIGAGLAGSEAAYQIAKRGVQVRLYEMRPVRQTPAHHTDKFAELVCSNSLRANTLTNAVGVIKEEMRLMDSVI
IRAADECSVPAGGALAVDRHEFAAKVTEYVKNHPNVTVMNEEITEIPEGPTIIATGPLTSPDLSAQLKELTGEDYFYFYD
AAAPIVEKDSIDMNKVYLKSRYDKGEAAYLNCPMTEEEFDRFYEALIAAETVPLKEFEKEIFFEGCMPVEVMASRGRQTL
VFGPMKPVGLEDPKTGKTPYAVVQLRQDDAAGTLYNIVGFQTHLKWGPQKEVLQLIPGLENAEIVRYGVMHRNTFINSPN
LLRPTYQYKQRDDLFFAGQMTGVEGYVESAASGLLAGINAARLVKGEEPVVLPPVTAMGSMANYITATNAKNFQPMNANF
GLFAPLEKKIKKKAERNEAYATRALETIRNFVNI
>Mature_433_residues
TTQVVNVIGAGLAGSEAAYQIAKRGVQVRLYEMRPVRQTPAHHTDKFAELVCSNSLRANTLTNAVGVIKEEMRLMDSVII
RAADECSVPAGGALAVDRHEFAAKVTEYVKNHPNVTVMNEEITEIPEGPTIIATGPLTSPDLSAQLKELTGEDYFYFYDA
AAPIVEKDSIDMNKVYLKSRYDKGEAAYLNCPMTEEEFDRFYEALIAAETVPLKEFEKEIFFEGCMPVEVMASRGRQTLV
FGPMKPVGLEDPKTGKTPYAVVQLRQDDAAGTLYNIVGFQTHLKWGPQKEVLQLIPGLENAEIVRYGVMHRNTFINSPNL
LRPTYQYKQRDDLFFAGQMTGVEGYVESAASGLLAGINAARLVKGEEPVVLPPVTAMGSMANYITATNAKNFQPMNANFG
LFAPLEKKIKKKAERNEAYATRALETIRNFVNI

Specific function: Catalyzes the folate-dependent formation of 5-methyl- uridine at position 54 (M-5-U54) in all tRNAs [H]

COG id: COG1206

COG function: function code J; NAD(FAD)-utilizing enzyme possibly involved in translation

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family. TrmFO subfamily [H]

Homologues:

Organism=Escherichia coli, GI2367273, Length=90, Percent_Identity=45.5555555555556, Blast_Score=73, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17534255, Length=131, Percent_Identity=34.3511450381679, Blast_Score=65, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6321202, Length=170, Percent_Identity=28.8235294117647, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004417
- InterPro:   IPR002218
- InterPro:   IPR020595 [H]

Pfam domain/function: PF01134 GIDA [H]

EC number: =2.1.1.74 [H]

Molecular weight: Translated: 48039; Mature: 47907

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTQVVNVIGAGLAGSEAAYQIAKRGVQVRLYEMRPVRQTPAHHTDKFAELVCSNSLRAN
CCCHHHHHHHCCCCCCHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCHH
TLTNAVGVIKEEMRLMDSVIIRAADECSVPAGGALAVDRHEFAAKVTEYVKNHPNVTVMN
HHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCEEEEHHHHHHHHHHHHHHCCCCEEEEC
EEITEIPEGPTIIATGPLTSPDLSAQLKELTGEDYFYFYDAAAPIVEKDSIDMNKVYLKS
CHHHHCCCCCEEEEECCCCCCCHHHHHHHCCCCCEEEEEECCCCCEECCCCCHHHHHHHH
RYDKGEAAYLNCPMTEEEFDRFYEALIAAETVPLKEFEKEIFFEGCMPVEVMASRGRQTL
CCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHCCCEEE
VFGPMKPVGLEDPKTGKTPYAVVQLRQDDAAGTLYNIVGFQTHLKWGPQKEVLQLIPGLE
EECCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHCCCCC
NAEIVRYGVMHRNTFINSPNLLRPTYQYKQRDDLFFAGQMTGVEGYVESAASGLLAGINA
CCCEEEEEHHHCCEECCCCCCCCCCHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCH
ARLVKGEEPVVLPPVTAMGSMANYITATNAKNFQPMNANFGLFAPLEKKIKKKAERNEAY
HHEECCCCCEEECCHHHHHHHHHHEEECCCCCCCCCCCCCCEECHHHHHHHHHHHHHHHH
ATRALETIRNFVNI
HHHHHHHHHHHHCC
>Mature Secondary Structure 
TTQVVNVIGAGLAGSEAAYQIAKRGVQVRLYEMRPVRQTPAHHTDKFAELVCSNSLRAN
CCHHHHHHHCCCCCCHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCHH
TLTNAVGVIKEEMRLMDSVIIRAADECSVPAGGALAVDRHEFAAKVTEYVKNHPNVTVMN
HHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCEEEEHHHHHHHHHHHHHHCCCCEEEEC
EEITEIPEGPTIIATGPLTSPDLSAQLKELTGEDYFYFYDAAAPIVEKDSIDMNKVYLKS
CHHHHCCCCCEEEEECCCCCCCHHHHHHHCCCCCEEEEEECCCCCEECCCCCHHHHHHHH
RYDKGEAAYLNCPMTEEEFDRFYEALIAAETVPLKEFEKEIFFEGCMPVEVMASRGRQTL
CCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHCCCEEE
VFGPMKPVGLEDPKTGKTPYAVVQLRQDDAAGTLYNIVGFQTHLKWGPQKEVLQLIPGLE
EECCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHCCCCC
NAEIVRYGVMHRNTFINSPNLLRPTYQYKQRDDLFFAGQMTGVEGYVESAASGLLAGINA
CCCEEEEEHHHCCEECCCCCCCCCCHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCH
ARLVKGEEPVVLPPVTAMGSMANYITATNAKNFQPMNANFGLFAPLEKKIKKKAERNEAY
HHEECCCCCEEECCHHHHHHHHHHEEECCCCCCCCCCCCCCEECHHHHHHHHHHHHHHHH
ATRALETIRNFVNI
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA