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The map label for this gene is topA

Identifier: 49478403

GI number: 49478403

Start: 3669976

End: 3672054

Strand: Reverse

Name: topA

Synonym: BT9727_3574

Alternate gene names: 49478403

Gene position: 3672054-3669976 (Counterclockwise)

Preceding gene: 49478404

Following gene: 49478916

Centisome position: 70.11

GC content: 39.15

Gene sequence:

>2079_bases
ATGTCAGATTACCTCGTAATCGTGGAGTCGCCTTCTAAGGCGAAGACCATTGAGAAATATTTAGGGAAAAAATACAAAGT
TGTCGCGTCTATGGGACACGTTCGCGATTTACCTAAAAGCCAAATGGGGATAGAAGTAAAGAACAACTTCACCCCGAAGT
ATATTACCATTCGTGGTAAAGGTCCCGTCTTAAAAGACTTAAAATCAGCGGCAAAAAAAGCAAAGAAAGTCTATCTCGCG
GCCGATCCAGACCGCGAAGGAGAAGCTATTGCTTGGCATTTAGCCAATACGTTAAATGTGGACGTTGAATCAGATTGCCG
AGTTGTGTTTAATGAGATTACAAAAGATGCAATTAAAGAATCATTCAAACATCCTCGTGCGATTAACATGGATTTAGTAG
ATGCACAACAAGCAAGACGTATACTAGATCGTCTTGTTGGTTACAATATTAGTCCTTTATTATGGAAGAAAGTAAAAAAA
GGATTAAGTGCAGGGCGCGTACAATCGGTAGCAGTTCGTTTAATTATCGAACGTGAAAGAGAAATTCAAAGTTTCGAGCC
TGAAGAATTCTGGACAATTAAAACAGAATTTGTGAAAGGGAAAGACACATTTGAAGCAAGCTTTTACGGTGTAGATGGTG
AAAAAGTTCAATTAACGAATGAAACACAAGTGAATGAAATAATTGAACAGCTGAAAGATAATGCGTTCTCAGTTGAAAAT
GTAACGCGAAAAGAGCGAAAACGTAATCCTGCATTACCATTTACAACATCTTCCTTGCAACAAGAGGCAGCGCGTAAGTT
AAACATGCGAGCAAAGAAAACGATGATGCTTGCGCAGCAATTGTATGAAGGGATCGATCTTGGAAAACAAGGAACTGTAG
GTCTTATTACGTATATGAGAACTGATTCAACACGTATCTCAGAAACAGCTCAAACAGAGGCACGTACTTATATTACTGAG
GCGTATGGTACGGAATACATAGGAGCAGAAAAGAAGAAAGAAACGAAGAAGTCGAACGCACAAGATGCGCATGAGGCAAT
TCGTCCTACTTCGGTAATGAGAAAGCCAGAGGAATTAAAGAGTTTCTTAAGTCGTGATCAACTTCGATTGTATAAATTGA
TTTGGGAGCGATTTGTTGCAAGTCAAATGGCGTCTGCTATAATGGATACTGTGACAGCGAGACTCATTAATAACAATGTT
CAGTTCCGTGCAAGTGGATCGGTTGTAAAGTTCCCAGGATTTATGAAAGTGTATGTAGAGTCGAAAGATGACGGGGCTGA
AGAAAAGGATAAGATGTTGCCACCTTTAGAAGTAGGGGAAACCGTATTTTCGAAGGATTTAGAACCGAAGCAGCACTTTA
CACAACCTCCGCCGCGCTATACTGAGGCTCGTCTAGTAAGAACACTTGAAGAGCTTGGAATTGGAAGACCGTCGACTTAC
GTACCGACACTTGAAACGATTCAAAAACGTGGATATGTAGGTTTGGATAATAAACGCTTCGTTCCAACTGAACTTGGTGA
AATAGTAATTGAACTTATTTTAGAGTTTTTCCCAGAAATTATTAACATTGAATTTACTGCTAACATGGAGCAAAGCCTCG
ATGAAATAGAAGAAGGAAATGCAAATTGGGTAAAAATTGTTGATGATTTCTACGTAGGGTTTGAACCGCGTTTAGAAAAA
GCGGAAAAAGAAATGCGTGAAGTAGAAATTAAAGATGAACCAGCTGGGGAAGACTGTGAATTATGTAATCACCCAATGGT
CTTTAAAATGGGTAAATACGGGAAATTTATGGCTTGCTCGAATTTCCCAGATTGTCGTAATACAAAACCGATTGTGAAAG
AAATCGGTGTTACTTGTCCGAAATGTGATAAAGGTCAAATTATTGAACGTCGTAGTAATAAAAAGAAACGTCTTTTCTAT
GGATGCGGTACGTATCCAGAGTGTGACTTTGTATCTTGGGATAAGCCGATTGGCCGTAAATGTCCGAAGTGTGAAGGTAT
GCTAGTAGAGAAGAAGTTGAAAAAAGGCGTACAAGTACAATGTATTTCGTGTGATTATGAAGAAGAACAACAAATGTGA

Upstream 100 bases:

>100_bases
AGATAGGCACTGTATGGTATATTCATTCTTGATTGACAAAAACAAGAGAAATCAATAAGATTAATGAAGACTTGAATCCA
CCTCTTAAGGAGGCACTAGC

Downstream 100 bases:

>100_bases
GCGTAACTGCTCACATTTTTTTCGGGAAGAAAAGGAAAGGTGATAAATATATGACAACACAAGTAGTAAACGTCATTGGC
GCAGGTCTTGCAGGAAGTGA

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 692; Mature: 691

Protein sequence:

>692_residues
MSDYLVIVESPSKAKTIEKYLGKKYKVVASMGHVRDLPKSQMGIEVKNNFTPKYITIRGKGPVLKDLKSAAKKAKKVYLA
ADPDREGEAIAWHLANTLNVDVESDCRVVFNEITKDAIKESFKHPRAINMDLVDAQQARRILDRLVGYNISPLLWKKVKK
GLSAGRVQSVAVRLIIEREREIQSFEPEEFWTIKTEFVKGKDTFEASFYGVDGEKVQLTNETQVNEIIEQLKDNAFSVEN
VTRKERKRNPALPFTTSSLQQEAARKLNMRAKKTMMLAQQLYEGIDLGKQGTVGLITYMRTDSTRISETAQTEARTYITE
AYGTEYIGAEKKKETKKSNAQDAHEAIRPTSVMRKPEELKSFLSRDQLRLYKLIWERFVASQMASAIMDTVTARLINNNV
QFRASGSVVKFPGFMKVYVESKDDGAEEKDKMLPPLEVGETVFSKDLEPKQHFTQPPPRYTEARLVRTLEELGIGRPSTY
VPTLETIQKRGYVGLDNKRFVPTELGEIVIELILEFFPEIINIEFTANMEQSLDEIEEGNANWVKIVDDFYVGFEPRLEK
AEKEMREVEIKDEPAGEDCELCNHPMVFKMGKYGKFMACSNFPDCRNTKPIVKEIGVTCPKCDKGQIIERRSNKKKRLFY
GCGTYPECDFVSWDKPIGRKCPKCEGMLVEKKLKKGVQVQCISCDYEEEQQM

Sequences:

>Translated_692_residues
MSDYLVIVESPSKAKTIEKYLGKKYKVVASMGHVRDLPKSQMGIEVKNNFTPKYITIRGKGPVLKDLKSAAKKAKKVYLA
ADPDREGEAIAWHLANTLNVDVESDCRVVFNEITKDAIKESFKHPRAINMDLVDAQQARRILDRLVGYNISPLLWKKVKK
GLSAGRVQSVAVRLIIEREREIQSFEPEEFWTIKTEFVKGKDTFEASFYGVDGEKVQLTNETQVNEIIEQLKDNAFSVEN
VTRKERKRNPALPFTTSSLQQEAARKLNMRAKKTMMLAQQLYEGIDLGKQGTVGLITYMRTDSTRISETAQTEARTYITE
AYGTEYIGAEKKKETKKSNAQDAHEAIRPTSVMRKPEELKSFLSRDQLRLYKLIWERFVASQMASAIMDTVTARLINNNV
QFRASGSVVKFPGFMKVYVESKDDGAEEKDKMLPPLEVGETVFSKDLEPKQHFTQPPPRYTEARLVRTLEELGIGRPSTY
VPTLETIQKRGYVGLDNKRFVPTELGEIVIELILEFFPEIINIEFTANMEQSLDEIEEGNANWVKIVDDFYVGFEPRLEK
AEKEMREVEIKDEPAGEDCELCNHPMVFKMGKYGKFMACSNFPDCRNTKPIVKEIGVTCPKCDKGQIIERRSNKKKRLFY
GCGTYPECDFVSWDKPIGRKCPKCEGMLVEKKLKKGVQVQCISCDYEEEQQM
>Mature_691_residues
SDYLVIVESPSKAKTIEKYLGKKYKVVASMGHVRDLPKSQMGIEVKNNFTPKYITIRGKGPVLKDLKSAAKKAKKVYLAA
DPDREGEAIAWHLANTLNVDVESDCRVVFNEITKDAIKESFKHPRAINMDLVDAQQARRILDRLVGYNISPLLWKKVKKG
LSAGRVQSVAVRLIIEREREIQSFEPEEFWTIKTEFVKGKDTFEASFYGVDGEKVQLTNETQVNEIIEQLKDNAFSVENV
TRKERKRNPALPFTTSSLQQEAARKLNMRAKKTMMLAQQLYEGIDLGKQGTVGLITYMRTDSTRISETAQTEARTYITEA
YGTEYIGAEKKKETKKSNAQDAHEAIRPTSVMRKPEELKSFLSRDQLRLYKLIWERFVASQMASAIMDTVTARLINNNVQ
FRASGSVVKFPGFMKVYVESKDDGAEEKDKMLPPLEVGETVFSKDLEPKQHFTQPPPRYTEARLVRTLEELGIGRPSTYV
PTLETIQKRGYVGLDNKRFVPTELGEIVIELILEFFPEIINIEFTANMEQSLDEIEEGNANWVKIVDDFYVGFEPRLEKA
EKEMREVEIKDEPAGEDCELCNHPMVFKMGKYGKFMACSNFPDCRNTKPIVKEIGVTCPKCDKGQIIERRSNKKKRLFYG
CGTYPECDFVSWDKPIGRKCPKCEGMLVEKKLKKGVQVQCISCDYEEEQQM

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=591, Percent_Identity=26.0575296108291, Blast_Score=130, Evalue=4e-30,
Organism=Homo sapiens, GI4507635, Length=673, Percent_Identity=22.7340267459138, Blast_Score=111, Evalue=2e-24,
Organism=Escherichia coli, GI1787529, Length=765, Percent_Identity=39.0849673202614, Blast_Score=503, Evalue=1e-143,
Organism=Escherichia coli, GI1788061, Length=600, Percent_Identity=22.1666666666667, Blast_Score=102, Evalue=7e-23,
Organism=Escherichia coli, GI48994931, Length=111, Percent_Identity=39.6396396396396, Blast_Score=97, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI32563869, Length=562, Percent_Identity=24.0213523131673, Blast_Score=139, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI17555378, Length=510, Percent_Identity=24.1176470588235, Blast_Score=117, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6323263, Length=550, Percent_Identity=26.3636363636364, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24585251, Length=626, Percent_Identity=24.4408945686901, Blast_Score=132, Evalue=5e-31,
Organism=Drosophila melanogaster, GI24640096, Length=582, Percent_Identity=24.914089347079, Blast_Score=123, Evalue=4e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 79079; Mature: 78948

Theoretical pI: Translated: 8.42; Mature: 8.42

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDYLVIVESPSKAKTIEKYLGKKYKVVASMGHVRDLPKSQMGIEVKNNFTPKYITIRGK
CCCEEEEEECCCHHHHHHHHHCCCEEHHHHHHHHHCCCCHHCCEEEECCCCCCEEEEECC
GPVLKDLKSAAKKAKKVYLAADPDREGEAIAWHLANTLNVDVESDCRVVFNEITKDAIKE
CCHHHHHHHHHHHHHEEEEEECCCCCCCEEEEEECCEEECCCCCCHHHHHHHHHHHHHHH
SFKHPRAINMDLVDAQQARRILDRLVGYNISPLLWKKVKKGLSAGRVQSVAVRLIIERER
HHCCCCCCCCHHHCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
EIQSFEPEEFWTIKTEFVKGKDTFEASFYGVDGEKVQLTNETQVNEIIEQLKDNAFSVEN
HHHCCCCCCEEEEEEEEECCCCCCEEEEECCCCCEEEECCCHHHHHHHHHHHCCCCCHHH
VTRKERKRNPALPFTTSSLQQEAARKLNMRAKKTMMLAQQLYEGIDLGKQGTVGLITYMR
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEE
TDSTRISETAQTEARTYITEAYGTEYIGAEKKKETKKSNAQDAHEAIRPTSVMRKPEELK
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHHHHHCCHHHHCCHHHHH
SFLSRDQLRLYKLIWERFVASQMASAIMDTVTARLINNNVQFRASGSVVKFPGFMKVYVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEECCCEEEEEEE
SKDDGAEEKDKMLPPLEVGETVFSKDLEPKQHFTQPPPRYTEARLVRTLEELGIGRPSTY
CCCCCCCHHHHCCCCHHHHHHHHHCCCCCHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCC
VPTLETIQKRGYVGLDNKRFVPTELGEIVIELILEFFPEIINIEFTANMEQSLDEIEEGN
CCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEEEEECCHHHHHHHHHCCC
ANWVKIVDDFYVGFEPRLEKAEKEMREVEIKDEPAGEDCELCNHPMVFKMGKYGKFMACS
CCEEEEEEHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCEEEECCCCCEEEEC
NFPDCRNTKPIVKEIGVTCPKCDKGQIIERRSNKKKRLFYGCGTYPECDFVSWDKPIGRK
CCCCCCCCCHHHHHHCCCCCCCCCCCHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCC
CPKCEGMLVEKKLKKGVQVQCISCDYEEEQQM
CCCCCCHHHHHHHHCCCEEEEEECCCHHHHCC
>Mature Secondary Structure 
SDYLVIVESPSKAKTIEKYLGKKYKVVASMGHVRDLPKSQMGIEVKNNFTPKYITIRGK
CCEEEEEECCCHHHHHHHHHCCCEEHHHHHHHHHCCCCHHCCEEEECCCCCCEEEEECC
GPVLKDLKSAAKKAKKVYLAADPDREGEAIAWHLANTLNVDVESDCRVVFNEITKDAIKE
CCHHHHHHHHHHHHHEEEEEECCCCCCCEEEEEECCEEECCCCCCHHHHHHHHHHHHHHH
SFKHPRAINMDLVDAQQARRILDRLVGYNISPLLWKKVKKGLSAGRVQSVAVRLIIERER
HHCCCCCCCCHHHCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
EIQSFEPEEFWTIKTEFVKGKDTFEASFYGVDGEKVQLTNETQVNEIIEQLKDNAFSVEN
HHHCCCCCCEEEEEEEEECCCCCCEEEEECCCCCEEEECCCHHHHHHHHHHHCCCCCHHH
VTRKERKRNPALPFTTSSLQQEAARKLNMRAKKTMMLAQQLYEGIDLGKQGTVGLITYMR
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEE
TDSTRISETAQTEARTYITEAYGTEYIGAEKKKETKKSNAQDAHEAIRPTSVMRKPEELK
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHHHHHCCHHHHCCHHHHH
SFLSRDQLRLYKLIWERFVASQMASAIMDTVTARLINNNVQFRASGSVVKFPGFMKVYVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEECCCEEEEEEE
SKDDGAEEKDKMLPPLEVGETVFSKDLEPKQHFTQPPPRYTEARLVRTLEELGIGRPSTY
CCCCCCCHHHHCCCCHHHHHHHHHCCCCCHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCC
VPTLETIQKRGYVGLDNKRFVPTELGEIVIELILEFFPEIINIEFTANMEQSLDEIEEGN
CCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEEEEECCHHHHHHHHHCCC
ANWVKIVDDFYVGFEPRLEKAEKEMREVEIKDEPAGEDCELCNHPMVFKMGKYGKFMACS
CCEEEEEEHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCEEEECCCCCEEEEC
NFPDCRNTKPIVKEIGVTCPKCDKGQIIERRSNKKKRLFYGCGTYPECDFVSWDKPIGRK
CCCCCCCCCHHHHHHCCCCCCCCCCCHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCC
CPKCEGMLVEKKLKKGVQVQCISCDYEEEQQM
CCCCCCHHHHHHHHCCCEEEEEECCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]