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The map label for this gene is menD

Identifier: 49478795

GI number: 49478795

Start: 4635147

End: 4636901

Strand: Reverse

Name: menD

Synonym: BT9727_4588

Alternate gene names: 49478795

Gene position: 4636901-4635147 (Counterclockwise)

Preceding gene: 49479345

Following gene: 49481645

Centisome position: 88.53

GC content: 40.63

Gene sequence:

>1755_bases
ATGAACAATCATATAGAAGCATTATCATATTATTTAGGCGCGTTCGTGGATGAACTGACGCGTCTAAATGTATGTGATGT
TGTCATTAGTCCAGGCTCACGGTCAACGCCGATTGCTCTACTAATGGAACAACATGAAGGAATGAACACATATTTACATG
TAGATGAAAGATCTGCAGGATTTTTTGCGCTCGGTATCGCAAAAGCGAAAAAACGTCCGGTCGCATTATTATGTACATCA
GGAACGGCTGCAGCGAACTATTATCCAGCTGTATGTGAAGCTTTTCATTCGCGAGTGCCGCTTATTGTCTTAACAGCAGA
TAGACCGCATGAATTAAGAGATGTCGGTGCACCACAAGCGATGAATCAAATTAATTTATACGGTACGTTTGTGAAGCAAT
TTACAGAGATGGCACTTCCAGAAGCGAGTGAAGCGATGTATCATTACGCTCGCATGACAACGCAGCGCATGATAGCAAGT
GCTTGTTTAGCACCGCAAGGACCTGTTCATCTTAATTTTCCAGTTCGTGAACCGCTTATCCCGGACTTCTCATTAGAAAG
TTTATGGGATAAAGGACGCGGGGAATATACAGGAGTAGTTCAGCAAGGGAACGCGGTGATGCCGAGTGAATATGTAGATT
CTCTTGTAGGGCGCCTTTCACATATGAAAAAGGGGCTTATTATTTGTGGAGATGATAGTCATTCAGAAATCGCAACGTTT
GCAACGCAACTAGCTGAAAAAACGGGATATCCAATATTAGCGGATCCACTTTCTAACATTCGTAGCGGACACCACGATAA
AACGATGGTAATCGACTGTTACGATACATTTTTACGAAATGAGCTGTTAAAGGAAACGTGGAAGCCGGACGTTTTAATTC
GCTTCGGTGGTATGCCTGTTTCAAAAGCATTAACGCAGTTCATCAAAAAACAAACGAAAGCTGTTCATATCGTAGTTGAC
GAATCAGGACAATGGAGAGATCCAGCTCTTGTTGCAACAGAAGTTGTTCAAGCAAGTGATATTGCATTTTGCAGTGCACT
AATAGAAAAAATGCCAGTTATGAAAAAGAATGATTGGTTCCGAATGTGGCAACATATAAACGAAAAAACGAAGGAAACGC
TTCGTGAAATGGAAACATATGATACGGCGTTTGAAGGAAGAGTCATTACGGATATTGTCCGCGTATTACCAGAAGGGGCA
ACGTTATTTGCGAGTAATAGTATGCCAATTCGTGATACAGATTCATTCTTTTTCACTTCGGATAAAACTATTCAAGTGAT
GGCTAACCGTGGTGTAAATGGGATTGATGGAATCATTTCGACAGCTTTAGGAGCGAGCATGATTTGCGATCCGCTCGTAT
TAGTAATCGGTGATTTATCATTTTATCACGATTTAAATGGATTATTAGCCGCAAAATTACATGAATTAAATATAACAATT
GTCGTTGTAAATAATGACGGTGGTGGTATTTTCTCATTCTTACCGCAATATGAGAAAAAGGAACATTTCGAATCGTTATT
TGGAACACCGATTGGGCTTGATTATGAGCATGTTGTTACAATGTATGGCGGTTCATTTAGCCGTGTAAATGGCTGGGAGC
AGTTCCGAGAAGAAGTACAAAAGGGTGCAACGACAGAAGGTTTACACGTTGTAGAAATTTGTACGAACCGTGATGAAAAC
GTAACATTGCACCGTAAATTATGGGCAAAAACGCAGGACGTAATTACTACATCGTTGCAAGGTGAATCAAAATGA

Upstream 100 bases:

>100_bases
CCGGTTGCGGAATTGTAATTGATTCAGTACCACAACTTGAATATGAAGAAACAAGTTTGAAGTTTAGACCGATGCTTGGT
GCTTTGGAGGAATTAATGAA

Downstream 100 bases:

>100_bases
ACGTAACGCTGCAAGGTGTATCGTATGAATATGAAGTAGTCGGAAGCGGAGAACCACTTCTACTTCTTCATGGTTTTACG
GGAAGCATGGAAACGTGGCG

Product: 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-carboxylate synthase

Products: NA

Alternate protein names: SEPHCHC synthase; Menaquinone biosynthesis protein menD [H]

Number of amino acids: Translated: 584; Mature: 584

Protein sequence:

>584_residues
MNNHIEALSYYLGAFVDELTRLNVCDVVISPGSRSTPIALLMEQHEGMNTYLHVDERSAGFFALGIAKAKKRPVALLCTS
GTAAANYYPAVCEAFHSRVPLIVLTADRPHELRDVGAPQAMNQINLYGTFVKQFTEMALPEASEAMYHYARMTTQRMIAS
ACLAPQGPVHLNFPVREPLIPDFSLESLWDKGRGEYTGVVQQGNAVMPSEYVDSLVGRLSHMKKGLIICGDDSHSEIATF
ATQLAEKTGYPILADPLSNIRSGHHDKTMVIDCYDTFLRNELLKETWKPDVLIRFGGMPVSKALTQFIKKQTKAVHIVVD
ESGQWRDPALVATEVVQASDIAFCSALIEKMPVMKKNDWFRMWQHINEKTKETLREMETYDTAFEGRVITDIVRVLPEGA
TLFASNSMPIRDTDSFFFTSDKTIQVMANRGVNGIDGIISTALGASMICDPLVLVIGDLSFYHDLNGLLAAKLHELNITI
VVVNNDGGGIFSFLPQYEKKEHFESLFGTPIGLDYEHVVTMYGGSFSRVNGWEQFREEVQKGATTEGLHVVEICTNRDEN
VTLHRKLWAKTQDVITTSLQGESK

Sequences:

>Translated_584_residues
MNNHIEALSYYLGAFVDELTRLNVCDVVISPGSRSTPIALLMEQHEGMNTYLHVDERSAGFFALGIAKAKKRPVALLCTS
GTAAANYYPAVCEAFHSRVPLIVLTADRPHELRDVGAPQAMNQINLYGTFVKQFTEMALPEASEAMYHYARMTTQRMIAS
ACLAPQGPVHLNFPVREPLIPDFSLESLWDKGRGEYTGVVQQGNAVMPSEYVDSLVGRLSHMKKGLIICGDDSHSEIATF
ATQLAEKTGYPILADPLSNIRSGHHDKTMVIDCYDTFLRNELLKETWKPDVLIRFGGMPVSKALTQFIKKQTKAVHIVVD
ESGQWRDPALVATEVVQASDIAFCSALIEKMPVMKKNDWFRMWQHINEKTKETLREMETYDTAFEGRVITDIVRVLPEGA
TLFASNSMPIRDTDSFFFTSDKTIQVMANRGVNGIDGIISTALGASMICDPLVLVIGDLSFYHDLNGLLAAKLHELNITI
VVVNNDGGGIFSFLPQYEKKEHFESLFGTPIGLDYEHVVTMYGGSFSRVNGWEQFREEVQKGATTEGLHVVEICTNRDEN
VTLHRKLWAKTQDVITTSLQGESK
>Mature_584_residues
MNNHIEALSYYLGAFVDELTRLNVCDVVISPGSRSTPIALLMEQHEGMNTYLHVDERSAGFFALGIAKAKKRPVALLCTS
GTAAANYYPAVCEAFHSRVPLIVLTADRPHELRDVGAPQAMNQINLYGTFVKQFTEMALPEASEAMYHYARMTTQRMIAS
ACLAPQGPVHLNFPVREPLIPDFSLESLWDKGRGEYTGVVQQGNAVMPSEYVDSLVGRLSHMKKGLIICGDDSHSEIATF
ATQLAEKTGYPILADPLSNIRSGHHDKTMVIDCYDTFLRNELLKETWKPDVLIRFGGMPVSKALTQFIKKQTKAVHIVVD
ESGQWRDPALVATEVVQASDIAFCSALIEKMPVMKKNDWFRMWQHINEKTKETLREMETYDTAFEGRVITDIVRVLPEGA
TLFASNSMPIRDTDSFFFTSDKTIQVMANRGVNGIDGIISTALGASMICDPLVLVIGDLSFYHDLNGLLAAKLHELNITI
VVVNNDGGGIFSFLPQYEKKEHFESLFGTPIGLDYEHVVTMYGGSFSRVNGWEQFREEVQKGATTEGLHVVEICTNRDEN
VTLHRKLWAKTQDVITTSLQGESK

Specific function: Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-ca

COG id: COG1165

COG function: function code H; 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family. MenD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788599, Length=567, Percent_Identity=28.5714285714286, Blast_Score=210, Evalue=2e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004433
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.9 [H]

Molecular weight: Translated: 65041; Mature: 65041

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS00041 HTH_ARAC_FAMILY_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNHIEALSYYLGAFVDELTRLNVCDVVISPGSRSTPIALLMEQHEGMNTYLHVDERSAG
CCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCEEEEHHCCCCCEEEEEECCCCC
FFALGIAKAKKRPVALLCTSGTAAANYYPAVCEAFHSRVPLIVLTADRPHELRDVGAPQA
EEEEEEHHHCCCCEEEEEECCCCCCCCCHHHHHHHHCCCCEEEEECCCCHHHHHCCCCHH
MNQINLYGTFVKQFTEMALPEASEAMYHYARMTTQRMIASACLAPQGPVHLNFPVREPLI
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCC
PDFSLESLWDKGRGEYTGVVQQGNAVMPSEYVDSLVGRLSHMKKGLIICGDDSHSEIATF
CCCCHHHHHHCCCCCEEEEEECCCEECCHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHH
ATQLAEKTGYPILADPLSNIRSGHHDKTMVIDCYDTFLRNELLKETWKPDVLIRFGGMPV
HHHHHHHCCCCEEECCHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHCCCCEEEEECCCCH
SKALTQFIKKQTKAVHIVVDESGQWRDPALVATEVVQASDIAFCSALIEKMPVMKKNDWF
HHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
RMWQHINEKTKETLREMETYDTAFEGRVITDIVRVLPEGATLFASNSMPIRDTDSFFFTS
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEECCCCCCCCCCCEEEEC
DKTIQVMANRGVNGIDGIISTALGASMICDPLVLVIGDLSFYHDLNGLLAAKLHELNITI
CCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEE
VVVNNDGGGIFSFLPQYEKKEHFESLFGTPIGLDYEHVVTMYGGSFSRVNGWEQFREEVQ
EEEECCCCCHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHEEECCCCHHCCCHHHHHHHHH
KGATTEGLHVVEICTNRDENVTLHRKLWAKTQDVITTSLQGESK
CCCCCCCEEEEEEECCCCCCEEEHHHHHHHHHHHHEECCCCCCC
>Mature Secondary Structure
MNNHIEALSYYLGAFVDELTRLNVCDVVISPGSRSTPIALLMEQHEGMNTYLHVDERSAG
CCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCEEEEHHCCCCCEEEEEECCCCC
FFALGIAKAKKRPVALLCTSGTAAANYYPAVCEAFHSRVPLIVLTADRPHELRDVGAPQA
EEEEEEHHHCCCCEEEEEECCCCCCCCCHHHHHHHHCCCCEEEEECCCCHHHHHCCCCHH
MNQINLYGTFVKQFTEMALPEASEAMYHYARMTTQRMIASACLAPQGPVHLNFPVREPLI
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCC
PDFSLESLWDKGRGEYTGVVQQGNAVMPSEYVDSLVGRLSHMKKGLIICGDDSHSEIATF
CCCCHHHHHHCCCCCEEEEEECCCEECCHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHH
ATQLAEKTGYPILADPLSNIRSGHHDKTMVIDCYDTFLRNELLKETWKPDVLIRFGGMPV
HHHHHHHCCCCEEECCHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHCCCCEEEEECCCCH
SKALTQFIKKQTKAVHIVVDESGQWRDPALVATEVVQASDIAFCSALIEKMPVMKKNDWF
HHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
RMWQHINEKTKETLREMETYDTAFEGRVITDIVRVLPEGATLFASNSMPIRDTDSFFFTS
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEECCCCCCCCCCCEEEEC
DKTIQVMANRGVNGIDGIISTALGASMICDPLVLVIGDLSFYHDLNGLLAAKLHELNITI
CCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEE
VVVNNDGGGIFSFLPQYEKKEHFESLFGTPIGLDYEHVVTMYGGSFSRVNGWEQFREEVQ
EEEECCCCCHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHEEECCCCHHCCCHHHHHHHHH
KGATTEGLHVVEICTNRDENVTLHRKLWAKTQDVITTSLQGESK
CCCCCCCEEEEEEECCCCCCEEEHHHHHHHHHHHHEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA