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The map label for this gene is menF

Identifier: 49479345

GI number: 49479345

Start: 4636898

End: 4638292

Strand: Reverse

Name: menF

Synonym: BT9727_4589

Alternate gene names: 49479345

Gene position: 4638292-4636898 (Counterclockwise)

Preceding gene: 49478797

Following gene: 49478795

Centisome position: 88.56

GC content: 37.99

Gene sequence:

>1395_bases
GTGATTCAAACGAAACAAATAGGCTTGCAAGAAGTTCTAAGTGCGGCCATTAAGCGTGCAACTGATGAAAAAATATTGGT
GAGCTTCGTAAAACAAATAGACTGGATGGATCCGCTTCTGTTTTATGCAGCAGGAAAAAGGATCTCATTCGAAAATAGAT
GTTATTTTGCAGACCCAGCGCAGCATGTAATATTTGCTGGAATTGGCTCTGTTTTCACTATAGCAACTGCTTCTCACAAA
CGCTTTCAAACTGCTCGTGACGAGTGGGATAAAGTAAAAGAGAAAGCATTTGTACAAAGGGAAAAATACGAGTTTGGAAC
AGGTCCTCTTTTATTTGGTGGATTTTCATTTGACCAAGAAAAAGAGAAAACAGATTTATGGAAAGAATTTGATGATACGA
CATTTTCACTACCGGCATTTTTATTAACTGTAAAAAATGAAAAAGCATGGTTAACAATGAATACATTCGTTTCAGCAACA
GATTGTGCAGAAACTCTTTATAACGAAATTGTTTCTTTAGAAGAGAAAATTTTTGAGGAGAGTAAATGTGCACTAGAAGG
ATCAAAATTAACAGTTACTTCTAAAGTAGAAGTGGATCCGAAAGGCTGGATGAAAGCCATTGAGAAAGTGCAAGATGAGA
TGAAGCAGGGGAACGTACAGAAGGTTGTATTAGCGAGGGAGCTAAAAGTAGAGATGGATCATCATATTGATTCTGCTCTT
GTTTTAGAAGCACTTCGCATTGGACAACCAGATTGTTACGTATTTTCTTTTGATTATAAAGGAGCATGCTTCTTAGGAGC
GACGCCAGAGAGATTAATTCGCAAAGAAGATGAGAAGTTTACTTCAATGTGTCTTGCTGGTTCAACTGGTCATGGTCAAT
CTATAGAAGAAAGTAAGCGAAATAGTAATGCGCTTCTTCATGATGAAAAGAATTTAGCTGAACACGGTTATGTTGTGAAC
ATGATTAGGTCGGTATTAAATGAGCACTGCGAATTCGTTAATATTCCAGAAAGTCCAGGTCTATTAACAACGAAAAACTT
AATTCATTTATATACGCCTGTAGAGGCAAAAGGCGATGCGAGTCTTTTAACGATGGTAGAAGAATTACATCCAACACCAG
CTCTTGGCGGGACACCTCGTTTAGAAGCGATGAAACTAATTCGTGATGTTGAATTGTTAGACAGAGGATTATATGGTGCA
CCGATTGGCTGGATAGATGATGAAGGAAATGGTGAATTTGCGGTTGCACTTCGCTGCGGATTATTAAATGGCGAGAAAGC
ATCCTTATTTGCCGGTTGCGGAATTGTAATTGATTCAGTACCACAACTTGAATATGAAGAAACAAGTTTGAAGTTTAGAC
CGATGCTTGGTGCTTTGGAGGAATTAATGAAATGA

Upstream 100 bases:

>100_bases
TTTCTCAGAAATGCTAAACGTTGACAGGCCTTTCGTTTGAGGTGTATCTTAAAATCAAGCGTAATTATGACTATTTTTCG
CCCATAGGGGGGAGACAATT

Downstream 100 bases:

>100_bases
ACAATCATATAGAAGCATTATCATATTATTTAGGCGCGTTCGTGGATGAACTGACGCGTCTAAATGTATGTGATGTTGTC
ATTAGTCCAGGCTCACGGTC

Product: menaquinone-specific isochorismate synthase

Products: NA

Alternate protein names: Isochorismate mutase [H]

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MIQTKQIGLQEVLSAAIKRATDEKILVSFVKQIDWMDPLLFYAAGKRISFENRCYFADPAQHVIFAGIGSVFTIATASHK
RFQTARDEWDKVKEKAFVQREKYEFGTGPLLFGGFSFDQEKEKTDLWKEFDDTTFSLPAFLLTVKNEKAWLTMNTFVSAT
DCAETLYNEIVSLEEKIFEESKCALEGSKLTVTSKVEVDPKGWMKAIEKVQDEMKQGNVQKVVLARELKVEMDHHIDSAL
VLEALRIGQPDCYVFSFDYKGACFLGATPERLIRKEDEKFTSMCLAGSTGHGQSIEESKRNSNALLHDEKNLAEHGYVVN
MIRSVLNEHCEFVNIPESPGLLTTKNLIHLYTPVEAKGDASLLTMVEELHPTPALGGTPRLEAMKLIRDVELLDRGLYGA
PIGWIDDEGNGEFAVALRCGLLNGEKASLFAGCGIVIDSVPQLEYEETSLKFRPMLGALEELMK

Sequences:

>Translated_464_residues
MIQTKQIGLQEVLSAAIKRATDEKILVSFVKQIDWMDPLLFYAAGKRISFENRCYFADPAQHVIFAGIGSVFTIATASHK
RFQTARDEWDKVKEKAFVQREKYEFGTGPLLFGGFSFDQEKEKTDLWKEFDDTTFSLPAFLLTVKNEKAWLTMNTFVSAT
DCAETLYNEIVSLEEKIFEESKCALEGSKLTVTSKVEVDPKGWMKAIEKVQDEMKQGNVQKVVLARELKVEMDHHIDSAL
VLEALRIGQPDCYVFSFDYKGACFLGATPERLIRKEDEKFTSMCLAGSTGHGQSIEESKRNSNALLHDEKNLAEHGYVVN
MIRSVLNEHCEFVNIPESPGLLTTKNLIHLYTPVEAKGDASLLTMVEELHPTPALGGTPRLEAMKLIRDVELLDRGLYGA
PIGWIDDEGNGEFAVALRCGLLNGEKASLFAGCGIVIDSVPQLEYEETSLKFRPMLGALEELMK
>Mature_464_residues
MIQTKQIGLQEVLSAAIKRATDEKILVSFVKQIDWMDPLLFYAAGKRISFENRCYFADPAQHVIFAGIGSVFTIATASHK
RFQTARDEWDKVKEKAFVQREKYEFGTGPLLFGGFSFDQEKEKTDLWKEFDDTTFSLPAFLLTVKNEKAWLTMNTFVSAT
DCAETLYNEIVSLEEKIFEESKCALEGSKLTVTSKVEVDPKGWMKAIEKVQDEMKQGNVQKVVLARELKVEMDHHIDSAL
VLEALRIGQPDCYVFSFDYKGACFLGATPERLIRKEDEKFTSMCLAGSTGHGQSIEESKRNSNALLHDEKNLAEHGYVVN
MIRSVLNEHCEFVNIPESPGLLTTKNLIHLYTPVEAKGDASLLTMVEELHPTPALGGTPRLEAMKLIRDVELLDRGLYGA
PIGWIDDEGNGEFAVALRCGLLNGEKASLFAGCGIVIDSVPQLEYEETSLKFRPMLGALEELMK

Specific function: Siderophore biosynthesis; enterobactin biosynthesis. [C]

COG id: COG1169

COG function: function code HQ; Isochorismate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786809, Length=242, Percent_Identity=41.7355371900826, Blast_Score=178, Evalue=8e-46,
Organism=Escherichia coli, GI87082077, Length=273, Percent_Identity=29.6703296703297, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1787518, Length=272, Percent_Identity=25.7352941176471, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6320935, Length=270, Percent_Identity=25.9259259259259, Blast_Score=81, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR015890
- InterPro:   IPR004561 [H]

Pfam domain/function: PF00425 Chorismate_bind [H]

EC number: =5.4.4.2 [H]

Molecular weight: Translated: 52019; Mature: 52019

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQTKQIGLQEVLSAAIKRATDEKILVSFVKQIDWMDPLLFYAAGKRISFENRCYFADPA
CCCCHHCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHCCCEECCCCCEEECCCH
QHVIFAGIGSVFTIATASHKRFQTARDEWDKVKEKAFVQREKYEFGTGPLLFGGFSFDQE
HHHHHHHHCHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCH
KEKTDLWKEFDDTTFSLPAFLLTVKNEKAWLTMNTFVSATDCAETLYNEIVSLEEKIFEE
HHHHHHHHHHCCCCEECCEEEEEEECCCEEEEEHHHCCHHHHHHHHHHHHHHHHHHHHHH
SKCALEGSKLTVTSKVEVDPKGWMKAIEKVQDEMKQGNVQKVVLARELKVEMDHHIDSAL
HHHHCCCCEEEEEEEEEECHHHHHHHHHHHHHHHHCCCHHEEHHHHHHHHHHHHCCHHHH
VLEALRIGQPDCYVFSFDYKGACFLGATPERLIRKEDEKFTSMCLAGSTGHGQSIEESKR
HHHHHHCCCCCEEEEEECCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHC
NSNALLHDEKNLAEHGYVVNMIRSVLNEHCEFVNIPESPGLLTTKNLIHLYTPVEAKGDA
CCCCEEECCHHHHHCCHHHHHHHHHHHHCCCEEECCCCCCCEEECCEEEEECCCCCCCCH
SLLTMVEELHPTPALGGTPRLEAMKLIRDVELLDRGLYGAPIGWIDDEGNGEFAVALRCG
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEE
LLNGEKASLFAGCGIVIDSVPQLEYEETSLKFRPMLGALEELMK
CCCCCCCHHHCCCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHC
>Mature Secondary Structure
MIQTKQIGLQEVLSAAIKRATDEKILVSFVKQIDWMDPLLFYAAGKRISFENRCYFADPA
CCCCHHCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHCCCEECCCCCEEECCCH
QHVIFAGIGSVFTIATASHKRFQTARDEWDKVKEKAFVQREKYEFGTGPLLFGGFSFDQE
HHHHHHHHCHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCH
KEKTDLWKEFDDTTFSLPAFLLTVKNEKAWLTMNTFVSATDCAETLYNEIVSLEEKIFEE
HHHHHHHHHHCCCCEECCEEEEEEECCCEEEEEHHHCCHHHHHHHHHHHHHHHHHHHHHH
SKCALEGSKLTVTSKVEVDPKGWMKAIEKVQDEMKQGNVQKVVLARELKVEMDHHIDSAL
HHHHCCCCEEEEEEEEEECHHHHHHHHHHHHHHHHCCCHHEEHHHHHHHHHHHHCCHHHH
VLEALRIGQPDCYVFSFDYKGACFLGATPERLIRKEDEKFTSMCLAGSTGHGQSIEESKR
HHHHHHCCCCCEEEEEECCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHC
NSNALLHDEKNLAEHGYVVNMIRSVLNEHCEFVNIPESPGLLTTKNLIHLYTPVEAKGDA
CCCCEEECCHHHHHCCHHHHHHHHHHHHCCCEEECCCCCCCEEECCEEEEECCCCCCCCH
SLLTMVEELHPTPALGGTPRLEAMKLIRDVELLDRGLYGAPIGWIDDEGNGEFAVALRCG
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEE
LLNGEKASLFAGCGIVIDSVPQLEYEETSLKFRPMLGALEELMK
CCCCCCCHHHCCCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1629163; 8566759; 9387221; 9384377; 3131310 [H]