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The map label for this gene is hemC

Identifier: 49478646

GI number: 49478646

Start: 4261366

End: 4262295

Strand: Reverse

Name: hemC

Synonym: BT9727_4197

Alternate gene names: 49478646

Gene position: 4262295-4261366 (Counterclockwise)

Preceding gene: 49478821

Following gene: 49478645

Centisome position: 81.38

GC content: 40.0

Gene sequence:

>930_bases
ATGCGCAAAATTATTGTAGGGTCACGAAAGAGTAAATTAGCATTAACGCAAACAAACTGGTTTATTGATCAGTTAAAAGC
GCTAGGTTTACCATATGAATTTGAAGTGAAAGAAATCGTCACAAAAGGTGATGTTATTTTAGATGTTACTCTTTCAAAAG
TAGGCGGAAAAGGTTTGTTTGTGAAAGAGATTGAACATGCGTTACTTACGAAAGAAATTGATATGGCTGTACATAGTATG
AAAGATATGCCGGCCGTACTTCCAGAAGGATTAATGATCGGTTGTACACCAAAGCGTGTTGACCCTCGTGATGCTTTTAT
CTCAAAAAGTGGAGCGTCGTTTAAAGAGTTAGCAGAAGGTGCTATTTTAGGTACGAGTAGTTTAAGACGTAGTGCACAGC
TGCTCGCTGCAAGACCAGATTTACAAGTGAAGTGGATTCGCGGAAATATCGATACACGTTTACGCAAATTAAAAGAAGAA
GATTACGATGCAATCATTTTAGCGACAGCTGGGTTACAAAGAATGGGCTGGGATGATGAAGTTATTACAGAACATTTAGA
TGAAACGTTATGTGTACCAGCTGTAGGGCAAGGCGCATTAGCGATTGAGTGCCGTGAAGATGATAAGGATTTACTACAGT
TGTTAGCGCATATAAATGATGCAGTAACAGAAAGAACAGTGGCAGCAGAACGGGTATTTCTTCATAAACTTGAAGGTGGA
TGCCAAGTTCCGATCGCTGGATATGCAACTCTTACAGAAAACGATGCAATCGAATTAACAGCTCTTGTCGGGTCTATGGA
CGGTTCTATCTTGTTGAAAGAGACAGTAGTAGGAACTGATCCAGAGAAAGTAGGATTAGAGGCTGCGGACCGTTTAATTA
AACAAGGCGCAAAAGAACTCATTCTTGCTGCAAATAAGGGGCAACAATAA

Upstream 100 bases:

>100_bases
GTGGAACATTGGGGCATTTCTCGTAATGCTAATTAATATTTTTCTATTAAGCAGTTTATCCAACTTCCACTTTTGGTATT
TATAATGAGGAGAGAAAATT

Downstream 100 bases:

>100_bases
ATATGAACGCTCTCGCTGGCAAAACGGTATTGATTACACGTGCCCAGCACCAAGCAAAACAAATGAGTGTAGCGGTGAAA
GAAAAGAGCGGAATTCCATT

Product: porphobilinogen deaminase

Products: NA

Alternate protein names: PBG; Hydroxymethylbilane synthase; HMBS; Pre-uroporphyrinogen synthase [H]

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MRKIIVGSRKSKLALTQTNWFIDQLKALGLPYEFEVKEIVTKGDVILDVTLSKVGGKGLFVKEIEHALLTKEIDMAVHSM
KDMPAVLPEGLMIGCTPKRVDPRDAFISKSGASFKELAEGAILGTSSLRRSAQLLAARPDLQVKWIRGNIDTRLRKLKEE
DYDAIILATAGLQRMGWDDEVITEHLDETLCVPAVGQGALAIECREDDKDLLQLLAHINDAVTERTVAAERVFLHKLEGG
CQVPIAGYATLTENDAIELTALVGSMDGSILLKETVVGTDPEKVGLEAADRLIKQGAKELILAANKGQQ

Sequences:

>Translated_309_residues
MRKIIVGSRKSKLALTQTNWFIDQLKALGLPYEFEVKEIVTKGDVILDVTLSKVGGKGLFVKEIEHALLTKEIDMAVHSM
KDMPAVLPEGLMIGCTPKRVDPRDAFISKSGASFKELAEGAILGTSSLRRSAQLLAARPDLQVKWIRGNIDTRLRKLKEE
DYDAIILATAGLQRMGWDDEVITEHLDETLCVPAVGQGALAIECREDDKDLLQLLAHINDAVTERTVAAERVFLHKLEGG
CQVPIAGYATLTENDAIELTALVGSMDGSILLKETVVGTDPEKVGLEAADRLIKQGAKELILAANKGQQ
>Mature_309_residues
MRKIIVGSRKSKLALTQTNWFIDQLKALGLPYEFEVKEIVTKGDVILDVTLSKVGGKGLFVKEIEHALLTKEIDMAVHSM
KDMPAVLPEGLMIGCTPKRVDPRDAFISKSGASFKELAEGAILGTSSLRRSAQLLAARPDLQVKWIRGNIDTRLRKLKEE
DYDAIILATAGLQRMGWDDEVITEHLDETLCVPAVGQGALAIECREDDKDLLQLLAHINDAVTERTVAAERVFLHKLEGG
CQVPIAGYATLTENDAIELTALVGSMDGSILLKETVVGTDPEKVGLEAADRLIKQGAKELILAANKGQQ

Specific function: Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps [H]

COG id: COG0181

COG function: function code H; Porphobilinogen deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMBS family [H]

Homologues:

Organism=Homo sapiens, GI66933009, Length=315, Percent_Identity=46.031746031746, Blast_Score=243, Evalue=1e-64,
Organism=Homo sapiens, GI20149500, Length=315, Percent_Identity=46.031746031746, Blast_Score=243, Evalue=1e-64,
Organism=Escherichia coli, GI48994974, Length=304, Percent_Identity=48.0263157894737, Blast_Score=257, Evalue=8e-70,
Organism=Saccharomyces cerevisiae, GI6319996, Length=316, Percent_Identity=38.2911392405063, Blast_Score=187, Evalue=1e-48,
Organism=Drosophila melanogaster, GI20130425, Length=309, Percent_Identity=41.747572815534, Blast_Score=205, Evalue=3e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000860
- InterPro:   IPR022419
- InterPro:   IPR022417
- InterPro:   IPR022418 [H]

Pfam domain/function: PF01379 Porphobil_deam; PF03900 Porphobil_deamC [H]

EC number: =2.5.1.61 [H]

Molecular weight: Translated: 33691; Mature: 33691

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS00533 PORPHOBILINOGEN_DEAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKIIVGSRKSKLALTQTNWFIDQLKALGLPYEFEVKEIVTKGDVILDVTLSKVGGKGLF
CCCEEECCCCCEEEEEEHHHHHHHHHHHCCCCEEHHHHHHCCCCEEEEEEEHHCCCCCEE
VKEIEHALLTKEIDMAVHSMKDMPAVLPEGLMIGCTPKRVDPRDAFISKSGASFKELAEG
HHHHHHHHHHHHHHHHHHHHHCCHHHCCCCEEEECCCCCCCCHHHHHCCCCCCHHHHHCC
AILGTSSLRRSAQLLAARPDLQVKWIRGNIDTRLRKLKEEDYDAIILATAGLQRMGWDDE
CEECHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHCCCCCEEEEEECCHHHCCCCHH
VITEHLDETLCVPAVGQGALAIECREDDKDLLQLLAHINDAVTERTVAAERVFLHKLEGG
HHHHHHHCCEECCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
CQVPIAGYATLTENDAIELTALVGSMDGSILLKETVVGTDPEKVGLEAADRLIKQGAKEL
CCCCCCCEEEECCCCCEEEEEEEECCCCCEEEEEEECCCCHHHHCHHHHHHHHHHCHHHE
ILAANKGQQ
EEECCCCCC
>Mature Secondary Structure
MRKIIVGSRKSKLALTQTNWFIDQLKALGLPYEFEVKEIVTKGDVILDVTLSKVGGKGLF
CCCEEECCCCCEEEEEEHHHHHHHHHHHCCCCEEHHHHHHCCCCEEEEEEEHHCCCCCEE
VKEIEHALLTKEIDMAVHSMKDMPAVLPEGLMIGCTPKRVDPRDAFISKSGASFKELAEG
HHHHHHHHHHHHHHHHHHHHHCCHHHCCCCEEEECCCCCCCCHHHHHCCCCCCHHHHHCC
AILGTSSLRRSAQLLAARPDLQVKWIRGNIDTRLRKLKEEDYDAIILATAGLQRMGWDDE
CEECHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHCCCCCEEEEEECCHHHCCCCHH
VITEHLDETLCVPAVGQGALAIECREDDKDLLQLLAHINDAVTERTVAAERVFLHKLEGG
HHHHHHHCCEECCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
CQVPIAGYATLTENDAIELTALVGSMDGSILLKETVVGTDPEKVGLEAADRLIKQGAKEL
CCCCCCCEEEECCCCCEEEEEEEECCCCCEEEEEEECCCCHHHHCHHHHHHHHHHCHHHE
ILAANKGQQ
EEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA