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The map label for this gene is spoIVFB

Identifier: 49478636

GI number: 49478636

Start: 4243166

End: 4244026

Strand: Reverse

Name: spoIVFB

Synonym: BT9727_4180

Alternate gene names: 49478636

Gene position: 4244026-4243166 (Counterclockwise)

Preceding gene: 49478637

Following gene: 49480672

Centisome position: 81.03

GC content: 33.68

Gene sequence:

>861_bases
TTGATTAAATATAGAGAGGTTTTAACAAAGATTTCAGTGCATCCGTTGTTTTGGGTTATTATTGTCATTGGTATTTTTAC
GGCACGTTTTAAAGAGTTACTATTGTTATTTTGTATCGTTCTTATCCATGAACTTGGGCATGCTTTTGCAGCGGCACACT
ATAATTGGCGCATCAAAAAAATTCAACTTTTACCGTTTGGTGGTGTAGCTGAGCTTGAGGAACATGGGAATAAGTCATTG
AAAGAGGAGCTTGTTGTCGTAATTGCAGGACCTATTCAACATATTTGGATGATGGCGATAGGCTATACGTTGTTTGAAGC
TGGTTGGCTTCATGCGGAATTATATTATTTCTTTATGTGGAATAATATAATTATTTTAGCATTTAATTTACTACCTATTT
GGCCACTTGATGGCGGGAAAGTATTGTTTAACGTATTATCATATCGTTTTCCTTATTTACAAGCACATGAAAAGATGATG
AAATTATCATGTGTTTTTTTTAGTGTCATACTAGGATGGCAGTTACTTTGGAATAGTAACAATATTATGATGTGGGTACT
GCTCATATTTTTAGCGGTGTCGTTATATCAAGAATGGAAACAAAGGCGGTATGCCTTTATGCGTTTTTTATTAGAACGTT
ATTATGGGAACAAAAGAGGAATTGAAAAGATTGCACCAATTGAGGTGAAAACAGAAGATCGTTTATATACAATCTTCACA
AAATTTCGTAGAGGGTATAAGCACTCTATTATCGTTCATGGAAAATATAAAGAGCATTACACATTGGATGAAAATGAATT
GCTTTATGCGTATTTTACTGAAAAACGAACAACTTCATCTGTTGAAGAATTAATCGGTTAG

Upstream 100 bases:

>100_bases
ATTGGAACGGTGAGTAATGATACAAACGATCAAAATGGTAAGTTTTATTTCGCAATAAAAAAGGATGAAAAATTTATTGA
CCCGATTCAGGTGATTTCAT

Downstream 100 bases:

>100_bases
TGTTGACGTTAGCACTAACCTTTTATTATTGTAAAAGCAAAGAAGATTATAAAGTGAGGAAAGAATTTTGAAAACGTTAT
ATATGAACTACGCTGGATCG

Product: stage IV sporulation protein FB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MIKYREVLTKISVHPLFWVIIVIGIFTARFKELLLLFCIVLIHELGHAFAAAHYNWRIKKIQLLPFGGVAELEEHGNKSL
KEELVVVIAGPIQHIWMMAIGYTLFEAGWLHAELYYFFMWNNIIILAFNLLPIWPLDGGKVLFNVLSYRFPYLQAHEKMM
KLSCVFFSVILGWQLLWNSNNIMMWVLLIFLAVSLYQEWKQRRYAFMRFLLERYYGNKRGIEKIAPIEVKTEDRLYTIFT
KFRRGYKHSIIVHGKYKEHYTLDENELLYAYFTEKRTTSSVEELIG

Sequences:

>Translated_286_residues
MIKYREVLTKISVHPLFWVIIVIGIFTARFKELLLLFCIVLIHELGHAFAAAHYNWRIKKIQLLPFGGVAELEEHGNKSL
KEELVVVIAGPIQHIWMMAIGYTLFEAGWLHAELYYFFMWNNIIILAFNLLPIWPLDGGKVLFNVLSYRFPYLQAHEKMM
KLSCVFFSVILGWQLLWNSNNIMMWVLLIFLAVSLYQEWKQRRYAFMRFLLERYYGNKRGIEKIAPIEVKTEDRLYTIFT
KFRRGYKHSIIVHGKYKEHYTLDENELLYAYFTEKRTTSSVEELIG
>Mature_286_residues
MIKYREVLTKISVHPLFWVIIVIGIFTARFKELLLLFCIVLIHELGHAFAAAHYNWRIKKIQLLPFGGVAELEEHGNKSL
KEELVVVIAGPIQHIWMMAIGYTLFEAGWLHAELYYFFMWNNIIILAFNLLPIWPLDGGKVLFNVLSYRFPYLQAHEKMM
KLSCVFFSVILGWQLLWNSNNIMMWVLLIFLAVSLYQEWKQRRYAFMRFLLERYYGNKRGIEKIAPIEVKTEDRLYTIFT
KFRRGYKHSIIVHGKYKEHYTLDENELLYAYFTEKRTTSSVEELIG

Specific function: Implicated in the coupling of mother cell to forespore gene expression. Required for spore formation. Processes the pro- sigma K factor [H]

COG id: COG1994

COG function: function code R; Zn-dependent proteases

Gene ontology:

Cell location: Forespore outer membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M50B family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008915 [H]

Pfam domain/function: PF02163 Peptidase_M50 [H]

EC number: NA

Molecular weight: Translated: 34034; Mature: 34034

Theoretical pI: Translated: 9.50; Mature: 9.50

Prosite motif: PS00142 ZINC_PROTEASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKYREVLTKISVHPLFWVIIVIGIFTARFKELLLLFCIVLIHELGHAFAAAHYNWRIKK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEE
IQLLPFGGVAELEEHGNKSLKEELVVVIAGPIQHIWMMAIGYTLFEAGWLHAELYYFFMW
EEEECCCCHHHHHHCCCCCHHHHEEEEEECHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
NNIIILAFNLLPIWPLDGGKVLFNVLSYRFPYLQAHEKMMKLSCVFFSVILGWQLLWNSN
CCCEEEEEECCEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHEECCC
NIMMWVLLIFLAVSLYQEWKQRRYAFMRFLLERYYGNKRGIEKIAPIEVKTEDRLYTIFT
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCHHHHHHH
KFRRGYKHSIIVHGKYKEHYTLDENELLYAYFTEKRTTSSVEELIG
HHHCCCCCEEEEEECCCCCEECCCCCEEEEEEECCCCHHHHHHHCC
>Mature Secondary Structure
MIKYREVLTKISVHPLFWVIIVIGIFTARFKELLLLFCIVLIHELGHAFAAAHYNWRIKK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEE
IQLLPFGGVAELEEHGNKSLKEELVVVIAGPIQHIWMMAIGYTLFEAGWLHAELYYFFMW
EEEECCCCHHHHHHCCCCCHHHHEEEEEECHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
NNIIILAFNLLPIWPLDGGKVLFNVLSYRFPYLQAHEKMMKLSCVFFSVILGWQLLWNSN
CCCEEEEEECCEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHEECCC
NIMMWVLLIFLAVSLYQEWKQRRYAFMRFLLERYYGNKRGIEKIAPIEVKTEDRLYTIFT
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCHHHHHHH
KFRRGYKHSIIVHGKYKEHYTLDENELLYAYFTEKRTTSSVEELIG
HHHCCCCCEEEEEECCCCCEECCCCCEEEEEEECCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1942049; 9384377; 10809718 [H]