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The map label for this gene is sigG

Identifier: 49478441

GI number: 49478441

Start: 3742791

End: 3743660

Strand: Reverse

Name: sigG

Synonym: BT9727_3645

Alternate gene names: 49478441

Gene position: 3743660-3742791 (Counterclockwise)

Preceding gene: 49478442

Following gene: 49478895

Centisome position: 71.48

GC content: 36.09

Gene sequence:

>870_bases
ATGAAGAATGAAGAGAAAACCGCATGCATAAAATCCCCTTCAAAGGAAATACTTTACACTGTACAGCAACTCCCGATAGG
AGGGAACACTTTGACGAGAAACAAAGTAGAAATTTGCGGTGTTGATACAGCTAAACTTCCCGTACTAAAAAATGATGAAA
TGCGTAAATTATTTCGTGAAATGCAAAGTGGAGAGATAAGCGCAAGAGAGAAATTAGTGAATGGAAACTTACGTCTTGTA
CTGAGCGTCATCCAAAGATTTAATAACAGAGGAGAATATGTTGACGATTTATTTCAAGTTGGTTGTATCGGACTTATGAA
ATCCATTGATAATTTTGATTTAGGCCAAAATGTAAAATTTTCAACGTATGCTGTGCCGATGATTATTGGGGAAATACGCA
GATATTTGCGTGATAACAATCCGATTCGCGTATCTCGCTCATTACGAGATATTGCGTATAAAGCGTTACAAGTAAGAGAA
AAGTTGATTGCAGAAAATTCAAAAGAACCAACAGCAATGGATATTGCAAAAGTGCTTGAAGTGACTCATGAAGAAATCGT
TTTTGCTTTAGATGCAATTCAAGATCCAGTTTCATTATTTGAACCGATTTATAACGATGGGGGAGATCCTATCTTTGTTA
TGGATCAGTTAAGTGATGAAAAACAAAAGGACGAGCAGTGGGTTGAAGAGCTAGCACTAAAAGAAGGAATGAAGCGTTTA
AATGATCGTGAGAAAATGATTATTCGCAAACGTTTCTTCCAAGGGAAAACACAAATGGAAGTTGCAGAAGAAATTGGGAT
TTCTCAAGCACAAGTGTCACGTTTAGAGAAATCAGCTATTAAACAAATGAATAAGACAATTCAAGGATAA

Upstream 100 bases:

>100_bases
AGATTACGAAAAGAATTTAATAAAATGGTGTAAAGAATAAGACCAGCCGTTATGTAGGCTGGTCTTATTCTCTTTCGTTG
TATGAGGAGAGACAACGAAA

Downstream 100 bases:

>100_bases
GGTTTCACCATTTATGGTGAAACCTTTTTATATGTCATATAGTTTGTTTACGCTCATAGTAGAGTAGCTGTGTTCATATA
ATGAAAACAATATGGGATTG

Product: sporulation sigma factor SigG

Products: NA

Alternate protein names: Stage III sporulation protein G [H]

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MKNEEKTACIKSPSKEILYTVQQLPIGGNTLTRNKVEICGVDTAKLPVLKNDEMRKLFREMQSGEISAREKLVNGNLRLV
LSVIQRFNNRGEYVDDLFQVGCIGLMKSIDNFDLGQNVKFSTYAVPMIIGEIRRYLRDNNPIRVSRSLRDIAYKALQVRE
KLIAENSKEPTAMDIAKVLEVTHEEIVFALDAIQDPVSLFEPIYNDGGDPIFVMDQLSDEKQKDEQWVEELALKEGMKRL
NDREKMIIRKRFFQGKTQMEVAEEIGISQAQVSRLEKSAIKQMNKTIQG

Sequences:

>Translated_289_residues
MKNEEKTACIKSPSKEILYTVQQLPIGGNTLTRNKVEICGVDTAKLPVLKNDEMRKLFREMQSGEISAREKLVNGNLRLV
LSVIQRFNNRGEYVDDLFQVGCIGLMKSIDNFDLGQNVKFSTYAVPMIIGEIRRYLRDNNPIRVSRSLRDIAYKALQVRE
KLIAENSKEPTAMDIAKVLEVTHEEIVFALDAIQDPVSLFEPIYNDGGDPIFVMDQLSDEKQKDEQWVEELALKEGMKRL
NDREKMIIRKRFFQGKTQMEVAEEIGISQAQVSRLEKSAIKQMNKTIQG
>Mature_289_residues
MKNEEKTACIKSPSKEILYTVQQLPIGGNTLTRNKVEICGVDTAKLPVLKNDEMRKLFREMQSGEISAREKLVNGNLRLV
LSVIQRFNNRGEYVDDLFQVGCIGLMKSIDNFDLGQNVKFSTYAVPMIIGEIRRYLRDNNPIRVSRSLRDIAYKALQVRE
KLIAENSKEPTAMDIAKVLEVTHEEIVFALDAIQDPVSLFEPIYNDGGDPIFVMDQLSDEKQKDEQWVEELALKEGMKRL
NDREKMIIRKRFFQGKTQMEVAEEIGISQAQVSRLEKSAIKQMNKTIQG

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of sporulation specific genes in the forespore [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=247, Percent_Identity=28.7449392712551, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI1789098, Length=300, Percent_Identity=24.3333333333333, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1789871, Length=261, Percent_Identity=27.2030651340996, Blast_Score=78, Evalue=6e-16,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014322
- InterPro:   IPR014212
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 33074; Mature: 33074

Theoretical pI: Translated: 7.20; Mature: 7.20

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNEEKTACIKSPSKEILYTVQQLPIGGNTLTRNKVEICGVDTAKLPVLKNDEMRKLFRE
CCCCHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCEEEECCCHHHCCCCCCHHHHHHHHH
MQSGEISAREKLVNGNLRLVLSVIQRFNNRGEYVDDLFQVGCIGLMKSIDNFDLGQNVKF
HHCCCHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
STYAVPMIIGEIRRYLRDNNPIRVSRSLRDIAYKALQVREKLIAENSKEPTAMDIAKVLE
HHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
VTHEEIVFALDAIQDPVSLFEPIYNDGGDPIFVMDQLSDEKQKDEQWVEELALKEGMKRL
HHHHHHHHHHHHHHCHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
NDREKMIIRKRFFQGKTQMEVAEEIGISQAQVSRLEKSAIKQMNKTIQG
HHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKNEEKTACIKSPSKEILYTVQQLPIGGNTLTRNKVEICGVDTAKLPVLKNDEMRKLFRE
CCCCHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCEEEECCCHHHCCCCCCHHHHHHHHH
MQSGEISAREKLVNGNLRLVLSVIQRFNNRGEYVDDLFQVGCIGLMKSIDNFDLGQNVKF
HHCCCHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
STYAVPMIIGEIRRYLRDNNPIRVSRSLRDIAYKALQVREKLIAENSKEPTAMDIAKVLE
HHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
VTHEEIVFALDAIQDPVSLFEPIYNDGGDPIFVMDQLSDEKQKDEQWVEELALKEGMKRL
HHHHHHHHHHHHHHCHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
NDREKMIIRKRFFQGKTQMEVAEEIGISQAQVSRLEKSAIKQMNKTIQG
HHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2497052; 2459711; 9384377 [H]