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The map label for this gene is pdhD2 [H]

Identifier: 49476301

GI number: 49476301

Start: 1906415

End: 1907821

Strand: Reverse

Name: pdhD2 [H]

Synonym: BH16520

Alternate gene names: 49476301

Gene position: 1907821-1906415 (Counterclockwise)

Preceding gene: 49476302

Following gene: 49476297

Centisome position: 98.8

GC content: 40.72

Gene sequence:

>1407_bases
ATGTCTTATGATGTAGTTGTGATCGGGGCAGGTCCAGGTGGTTATGTGGCAGCAATCAAAGCAGCACAGTTGGGGCTTAA
AACAGCGATTATTGAAAAACGTATGACACTAGGTGGTACATGTCTCAATGTTGGCTGTATTCCTTCTAAAGCATTGTTGC
ATGCTTCAGAAGTCTTTGCTGAGACGCAACATGGTTTTGAAACGCTTGGTATTTCTATTGCAAAATCTAAGCTTAATCTT
GAACAAATGATGGCGCATAAAAAAGCTGTTGTCACTGCAAATACGAGTGGTGTTTCTTTTTTGATGAAGAAAAATAAAAT
TGACACTTTTTTTGGTACTGCCAAAATTTTAAACGCAGGTCAGATCGAAGTTGTTGCAAGGGATGGTAACAAACAAACGA
TTGAAACAAAAAATATTATTATTGCTACTGGTTCAGAGAGCTCAGGCATTCCGGGTGTGAATGTTGAAATTGATGAAAAG
GTTATTGTATCCTCTACAGGAGCACTTGCTCTTGAAAAAGTTCCAACGCGTATGATTGTTGTGGGCGCTGGTGTTATTGG
TTCAGAGCTTGGTTCGGTTTGGAGCCGTTTAGGGGCTAAAGTTACCATTATTGAATATCTTAATAAAGTTTTAGGATCAA
TGGATGGTGAAGTCTCACGGCAATTCCAAAAAATAATGGAAAAACAAGGAATTGAATATAAGATTGGTGCAAAAGTAACA
GCTATTACACAATCTGGTTCAGTTGCTCAGGTGACTTTTGAGGCTGTTAAGGGTGGTCCTTCTGAAACTCTAGAAGCTGA
TGTCGTGCTTATTGCTACTGGACGTTTTCCATATACAGAAGGACTTGGCTTGGAAGAGGCTGGTGTTCAGTTGGATGAAC
GGGGGTTTATTACAATTGATGCGCATTGGCAAACGAATATTCCAGGAGTTTATGCGATTGGTGATGTCGTTAAAGGACCA
ATGTTGGCACATAAGGCAGAGGAAGAAGGCGTTGCTGTGGCAGAAATTTTAGCGGGTCAAAAGGGACATGTTAATTTTGA
TGTCATTCCTAGTGTTGTTTACACACAGCCAGAAATTGCTAGTGTAGGCAAGACAGAAGAAGAACTTAAAGCAGCCGGTA
TCGATTATAATGTTGGAAAATTTCCTTTCATGGCCAATGGTCGTGCGCGTGCCATGCAAAAGAGTGATGGATTTGTTAAA
ATTCTTGCTGATAAAAAGACAGATCGGGTTTTGGGCGGACATATTTTGGGGTTTGGTGCTGGTGAAATGATTCATGAAAT
TGCAGTTTTAATGGAATTTGGTGGTTCATCAGAAGATCTGGGACGTTGTTGTCATGCGCATCCTACTTTATCAGAGGCAG
TGCGCGAAGCAGCTTTGGCAACATTTGCTAAACCCCTTCATATCTAG

Upstream 100 bases:

>100_bases
CTGTCACTTTCCTTGTGCGTGTTAAGGAAAGTTTAGAAGATCCGGAACGCCTGGTTCTTGACTTGTAAAAGTACAGTTTT
CAGGATGAAAGGAAAAACAG

Downstream 100 bases:

>100_bases
TTTCTGTGAACCTTCGTTAAAACTTGAGAGTAGAAGAGTTGGTAACATTCCATTTGTAACAGGTATGTTACTAAGGGCAA
GTTCTCTCTCAAGTAAAGGA

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes [H]

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MSYDVVVIGAGPGGYVAAIKAAQLGLKTAIIEKRMTLGGTCLNVGCIPSKALLHASEVFAETQHGFETLGISIAKSKLNL
EQMMAHKKAVVTANTSGVSFLMKKNKIDTFFGTAKILNAGQIEVVARDGNKQTIETKNIIIATGSESSGIPGVNVEIDEK
VIVSSTGALALEKVPTRMIVVGAGVIGSELGSVWSRLGAKVTIIEYLNKVLGSMDGEVSRQFQKIMEKQGIEYKIGAKVT
AITQSGSVAQVTFEAVKGGPSETLEADVVLIATGRFPYTEGLGLEEAGVQLDERGFITIDAHWQTNIPGVYAIGDVVKGP
MLAHKAEEEGVAVAEILAGQKGHVNFDVIPSVVYTQPEIASVGKTEEELKAAGIDYNVGKFPFMANGRARAMQKSDGFVK
ILADKKTDRVLGGHILGFGAGEMIHEIAVLMEFGGSSEDLGRCCHAHPTLSEAVREAALATFAKPLHI

Sequences:

>Translated_468_residues
MSYDVVVIGAGPGGYVAAIKAAQLGLKTAIIEKRMTLGGTCLNVGCIPSKALLHASEVFAETQHGFETLGISIAKSKLNL
EQMMAHKKAVVTANTSGVSFLMKKNKIDTFFGTAKILNAGQIEVVARDGNKQTIETKNIIIATGSESSGIPGVNVEIDEK
VIVSSTGALALEKVPTRMIVVGAGVIGSELGSVWSRLGAKVTIIEYLNKVLGSMDGEVSRQFQKIMEKQGIEYKIGAKVT
AITQSGSVAQVTFEAVKGGPSETLEADVVLIATGRFPYTEGLGLEEAGVQLDERGFITIDAHWQTNIPGVYAIGDVVKGP
MLAHKAEEEGVAVAEILAGQKGHVNFDVIPSVVYTQPEIASVGKTEEELKAAGIDYNVGKFPFMANGRARAMQKSDGFVK
ILADKKTDRVLGGHILGFGAGEMIHEIAVLMEFGGSSEDLGRCCHAHPTLSEAVREAALATFAKPLHI
>Mature_467_residues
SYDVVVIGAGPGGYVAAIKAAQLGLKTAIIEKRMTLGGTCLNVGCIPSKALLHASEVFAETQHGFETLGISIAKSKLNLE
QMMAHKKAVVTANTSGVSFLMKKNKIDTFFGTAKILNAGQIEVVARDGNKQTIETKNIIIATGSESSGIPGVNVEIDEKV
IVSSTGALALEKVPTRMIVVGAGVIGSELGSVWSRLGAKVTIIEYLNKVLGSMDGEVSRQFQKIMEKQGIEYKIGAKVTA
ITQSGSVAQVTFEAVKGGPSETLEADVVLIATGRFPYTEGLGLEEAGVQLDERGFITIDAHWQTNIPGVYAIGDVVKGPM
LAHKAEEEGVAVAEILAGQKGHVNFDVIPSVVYTQPEIASVGKTEEELKAAGIDYNVGKFPFMANGRARAMQKSDGFVKI
LADKKTDRVLGGHILGFGAGEMIHEIAVLMEFGGSSEDLGRCCHAHPTLSEAVREAALATFAKPLHI

Specific function: Lipoamide dehydrogenase is a component of the alpha- ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=470, Percent_Identity=55.7446808510638, Blast_Score=513, Evalue=1e-145,
Organism=Homo sapiens, GI50301238, Length=467, Percent_Identity=32.762312633833, Blast_Score=195, Evalue=8e-50,
Organism=Homo sapiens, GI22035672, Length=476, Percent_Identity=30.0420168067227, Blast_Score=141, Evalue=2e-33,
Organism=Homo sapiens, GI33519430, Length=476, Percent_Identity=27.1008403361345, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI33519428, Length=476, Percent_Identity=27.1008403361345, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI33519426, Length=476, Percent_Identity=27.1008403361345, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI148277065, Length=476, Percent_Identity=27.1008403361345, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI148277071, Length=476, Percent_Identity=27.1008403361345, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI291045266, Length=460, Percent_Identity=26.5217391304348, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI291045268, Length=454, Percent_Identity=25.7709251101322, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI226437568, Length=205, Percent_Identity=29.7560975609756, Blast_Score=76, Evalue=7e-14,
Organism=Homo sapiens, GI21389617, Length=205, Percent_Identity=29.7560975609756, Blast_Score=76, Evalue=7e-14,
Organism=Homo sapiens, GI65787454, Length=205, Percent_Identity=29.7560975609756, Blast_Score=76, Evalue=8e-14,
Organism=Escherichia coli, GI1786307, Length=454, Percent_Identity=41.8502202643172, Blast_Score=341, Evalue=5e-95,
Organism=Escherichia coli, GI87082354, Length=463, Percent_Identity=31.1015118790497, Blast_Score=213, Evalue=3e-56,
Organism=Escherichia coli, GI1789915, Length=439, Percent_Identity=32.5740318906606, Blast_Score=206, Evalue=3e-54,
Organism=Escherichia coli, GI87081717, Length=467, Percent_Identity=30.406852248394, Blast_Score=191, Evalue=1e-49,
Organism=Escherichia coli, GI1789065, Length=143, Percent_Identity=31.4685314685315, Blast_Score=64, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI32565766, Length=461, Percent_Identity=53.7960954446855, Blast_Score=496, Evalue=1e-140,
Organism=Caenorhabditis elegans, GI17557007, Length=472, Percent_Identity=29.4491525423729, Blast_Score=153, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI71983429, Length=442, Percent_Identity=27.6018099547511, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI71983419, Length=442, Percent_Identity=27.6018099547511, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI71982272, Length=485, Percent_Identity=25.7731958762887, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17559934, Length=261, Percent_Identity=24.904214559387, Blast_Score=76, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6321091, Length=475, Percent_Identity=50.3157894736842, Blast_Score=466, Evalue=1e-132,
Organism=Saccharomyces cerevisiae, GI6325240, Length=470, Percent_Identity=32.1276595744681, Blast_Score=250, Evalue=4e-67,
Organism=Saccharomyces cerevisiae, GI6325166, Length=467, Percent_Identity=28.2655246252677, Blast_Score=175, Evalue=1e-44,
Organism=Drosophila melanogaster, GI21358499, Length=468, Percent_Identity=54.7008547008547, Blast_Score=521, Evalue=1e-148,
Organism=Drosophila melanogaster, GI24640553, Length=483, Percent_Identity=26.7080745341615, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24640549, Length=483, Percent_Identity=26.7080745341615, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24640551, Length=483, Percent_Identity=26.7080745341615, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI17737741, Length=480, Percent_Identity=27.5, Blast_Score=120, Evalue=2e-27,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 49420; Mature: 49288

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYDVVVIGAGPGGYVAAIKAAQLGLKTAIIEKRMTLGGTCLNVGCIPSKALLHASEVFA
CCEEEEEEECCCCCCEEEHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHH
ETQHGFETLGISIAKSKLNLEQMMAHKKAVVTANTSGVSFLMKKNKIDTFFGTAKILNAG
HHHCCHHHHHHEEEHHHCCHHHHHHHCCEEEEECCCCEEEEEECCCCCHHCCHHHHCCCC
QIEVVARDGNKQTIETKNIIIATGSESSGIPGVNVEIDEKVIVSSTGALALEKVPTRMIV
EEEEEEECCCCCEEEECEEEEEECCCCCCCCCCEEEECCEEEEECCCCEEECCCCCEEEE
VGAGVIGSELGSVWSRLGAKVTIIEYLNKVLGSMDGEVSRQFQKIMEKQGIEYKIGAKVT
EECCHHHHHHHHHHHHHCCEEEHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEECCEEE
AITQSGSVAQVTFEAVKGGPSETLEADVVLIATGRFPYTEGLGLEEAGVQLDERGFITID
EEECCCCEEEEEEEHHCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHCCEECCCCEEEEE
AHWQTNIPGVYAIGDVVKGPMLAHKAEEEGVAVAEILAGQKGHVNFDVIPSVVYTQPEIA
ECCCCCCCCEEEEHHHHCCCHHHHCCCCCCCHHHHHHCCCCCCEEHEECCCEEECCCCHH
SVGKTEEELKAAGIDYNVGKFPFMANGRARAMQKSDGFVKILADKKTDRVLGGHILGFGA
HCCCCHHHHHHCCCCCCCCCCCEEECCHHHHHCCCCCEEEEEECCCCCHHHCCEEEECCH
GEMIHEIAVLMEFGGSSEDLGRCCHAHPTLSEAVREAALATFAKPLHI
HHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SYDVVVIGAGPGGYVAAIKAAQLGLKTAIIEKRMTLGGTCLNVGCIPSKALLHASEVFA
CEEEEEEECCCCCCEEEHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHH
ETQHGFETLGISIAKSKLNLEQMMAHKKAVVTANTSGVSFLMKKNKIDTFFGTAKILNAG
HHHCCHHHHHHEEEHHHCCHHHHHHHCCEEEEECCCCEEEEEECCCCCHHCCHHHHCCCC
QIEVVARDGNKQTIETKNIIIATGSESSGIPGVNVEIDEKVIVSSTGALALEKVPTRMIV
EEEEEEECCCCCEEEECEEEEEECCCCCCCCCCEEEECCEEEEECCCCEEECCCCCEEEE
VGAGVIGSELGSVWSRLGAKVTIIEYLNKVLGSMDGEVSRQFQKIMEKQGIEYKIGAKVT
EECCHHHHHHHHHHHHHCCEEEHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEECCEEE
AITQSGSVAQVTFEAVKGGPSETLEADVVLIATGRFPYTEGLGLEEAGVQLDERGFITID
EEECCCCEEEEEEEHHCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHCCEECCCCEEEEE
AHWQTNIPGVYAIGDVVKGPMLAHKAEEEGVAVAEILAGQKGHVNFDVIPSVVYTQPEIA
ECCCCCCCCEEEEHHHHCCCHHHHCCCCCCCHHHHHHCCCCCCEEHEECCCEEECCCCHH
SVGKTEEELKAAGIDYNVGKFPFMANGRARAMQKSDGFVKILADKKTDRVLGGHILGFGA
HCCCCHHHHHHCCCCCCCCCCCEEECCHHHHHCCCCCEEEEEECCCCCHHHCCEEEECCH
GEMIHEIAVLMEFGGSSEDLGRCCHAHPTLSEAVREAALATFAKPLHI
HHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9226266 [H]