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The map label for this gene is sucB [H]

Identifier: 49476302

GI number: 49476302

Start: 1907854

End: 1909074

Strand: Reverse

Name: sucB [H]

Synonym: BH16530

Alternate gene names: 49476302

Gene position: 1909074-1907854 (Counterclockwise)

Preceding gene: 49476303

Following gene: 49476301

Centisome position: 98.86

GC content: 42.42

Gene sequence:

>1221_bases
ATGACTACTGAAATCCGTGTTCCTACTTTAGGCGAATCAGTTACTGAGGCGACAGTTGGTAAGTGGTTTAAAAAGCTTGG
CGAAGCTGTGGCTGTGGATGAACCCTTGATTGAATTAGAAACTGATAAGGTAACCGTTGAGGTTCCTTCGCCTGTTGCAG
GAAAATTATCTGAAATCATCGCAAAAGAAGGTGATACGGTTGAAGTAAAAGCTCTGTTAGGATTAGTGGAAGCTGGAGCT
GCTGGTATTTCCCAATCATTTTCACCTTCTGCTACGCCTATTCCAGAAGTGCCATCTGAATTGAAGCAGTCTTCTTCCAG
TGGTGCAATGCAGAAAGATACAATGCCGCCCTCTCCTTCAGCAGCAAAATTGATGGCTGAAAATAATATTGCAAAAAGCA
ATATTTCAGGTTCTGGAAAACGTGGACAAATCCTCAAAGAAGATGTTCTTGGTGTCTTAGAACAAGAAGTAAAAGCGCCA
TCTGTTTCTGCAGCTTCCTCTTCGGCTTCTCTTGTTCAAGAAAAGCATGAAGAGCGGGTTCGTATGACAAAATTGCGTCA
AACAATTGCGCGTCGTCTTAAAGATGCACAGAATACAGCAGCAATGTTAACTACATTTAATGAGGTTGATATGTCGGCTG
TGATGGATTTGCGCAAGCGTTATAAGGATCTTTTTGAAAAGAAACATGGTGTTAAACTCGGCTTTATGGGATTTTTTACC
AAGGCTGTTTGTCATGCATTAAAAGAACTTCCTGCTGTTAATGCGGAAATTGATGGAACGGATATTGTTTACAAAAATTA
CGTCAATGTTGGAATTGCTGTTGGAACGGATAAAGGACTTGTCGTTCCGGTAGTGCGTCATGCAGATCAGATGTCGTTAG
CAGAGATTGAAAAGGAAATTGGTCGTTTGGGGCGTCTTGCTCGTGATGGAAAATTAGCGGTTTCTGATATGCAAGGTGGA
ACTTTTACCATTACCAATGGGGGTGTTTATGGTTCGTTAATGTCGACACCGATTTTAAATGCACCACAGTCCGGTATCTT
GGGAATGCACGCGATTAAAGAACGAGCAATGGTTGTTGGCGGGCAAATAGTCATTCGCCCCATGATGTATCTGGCGCTTT
CATATGATCACCGTATTGTAGATGGACAAGAGGCTGTCACTTTCCTTGTGCGTGTTAAGGAAAGTTTAGAAGATCCGGAA
CGCCTGGTTCTTGACTTGTAA

Upstream 100 bases:

>100_bases
AGCCACTGGATTGATGGTACAACATGCTGAACAGCTTGCCGCGTTTCTTGAAGACGCGTTAGGTTCTTAACTTAATTACT
CTGATGTATGGAAAAATATT

Downstream 100 bases:

>100_bases
AAGTACAGTTTTCAGGATGAAAGGAAAAACAGATGTCTTATGATGTAGTTGTGATCGGGGCAGGTCCAGGTGGTTATGTG
GCAGCAATCAAAGCAGCACA

Product: dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 406; Mature: 405

Protein sequence:

>406_residues
MTTEIRVPTLGESVTEATVGKWFKKLGEAVAVDEPLIELETDKVTVEVPSPVAGKLSEIIAKEGDTVEVKALLGLVEAGA
AGISQSFSPSATPIPEVPSELKQSSSSGAMQKDTMPPSPSAAKLMAENNIAKSNISGSGKRGQILKEDVLGVLEQEVKAP
SVSAASSSASLVQEKHEERVRMTKLRQTIARRLKDAQNTAAMLTTFNEVDMSAVMDLRKRYKDLFEKKHGVKLGFMGFFT
KAVCHALKELPAVNAEIDGTDIVYKNYVNVGIAVGTDKGLVVPVVRHADQMSLAEIEKEIGRLGRLARDGKLAVSDMQGG
TFTITNGGVYGSLMSTPILNAPQSGILGMHAIKERAMVVGGQIVIRPMMYLALSYDHRIVDGQEAVTFLVRVKESLEDPE
RLVLDL

Sequences:

>Translated_406_residues
MTTEIRVPTLGESVTEATVGKWFKKLGEAVAVDEPLIELETDKVTVEVPSPVAGKLSEIIAKEGDTVEVKALLGLVEAGA
AGISQSFSPSATPIPEVPSELKQSSSSGAMQKDTMPPSPSAAKLMAENNIAKSNISGSGKRGQILKEDVLGVLEQEVKAP
SVSAASSSASLVQEKHEERVRMTKLRQTIARRLKDAQNTAAMLTTFNEVDMSAVMDLRKRYKDLFEKKHGVKLGFMGFFT
KAVCHALKELPAVNAEIDGTDIVYKNYVNVGIAVGTDKGLVVPVVRHADQMSLAEIEKEIGRLGRLARDGKLAVSDMQGG
TFTITNGGVYGSLMSTPILNAPQSGILGMHAIKERAMVVGGQIVIRPMMYLALSYDHRIVDGQEAVTFLVRVKESLEDPE
RLVLDL
>Mature_405_residues
TTEIRVPTLGESVTEATVGKWFKKLGEAVAVDEPLIELETDKVTVEVPSPVAGKLSEIIAKEGDTVEVKALLGLVEAGAA
GISQSFSPSATPIPEVPSELKQSSSSGAMQKDTMPPSPSAAKLMAENNIAKSNISGSGKRGQILKEDVLGVLEQEVKAPS
VSAASSSASLVQEKHEERVRMTKLRQTIARRLKDAQNTAAMLTTFNEVDMSAVMDLRKRYKDLFEKKHGVKLGFMGFFTK
AVCHALKELPAVNAEIDGTDIVYKNYVNVGIAVGTDKGLVVPVVRHADQMSLAEIEKEIGRLGRLARDGKLAVSDMQGGT
FTITNGGVYGSLMSTPILNAPQSGILGMHAIKERAMVVGGQIVIRPMMYLALSYDHRIVDGQEAVTFLVRVKESLEDPER
LVLDL

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and l

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=234, Percent_Identity=56.4102564102564, Blast_Score=293, Evalue=2e-79,
Organism=Homo sapiens, GI110671329, Length=424, Percent_Identity=28.3018867924528, Blast_Score=165, Evalue=6e-41,
Organism=Homo sapiens, GI203098753, Length=448, Percent_Identity=27.4553571428571, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI203098816, Length=448, Percent_Identity=27.4553571428571, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI31711992, Length=432, Percent_Identity=27.0833333333333, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI260898739, Length=159, Percent_Identity=32.7044025157233, Blast_Score=96, Evalue=7e-20,
Organism=Escherichia coli, GI1786946, Length=408, Percent_Identity=50.9803921568627, Blast_Score=392, Evalue=1e-110,
Organism=Escherichia coli, GI1786305, Length=425, Percent_Identity=28.9411764705882, Blast_Score=177, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI25146366, Length=403, Percent_Identity=43.9205955334988, Blast_Score=328, Evalue=4e-90,
Organism=Caenorhabditis elegans, GI17560088, Length=436, Percent_Identity=30.9633027522936, Blast_Score=159, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17537937, Length=421, Percent_Identity=26.1282660332542, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17538894, Length=305, Percent_Identity=28.8524590163934, Blast_Score=125, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6320352, Length=403, Percent_Identity=46.4019851116625, Blast_Score=346, Evalue=3e-96,
Organism=Saccharomyces cerevisiae, GI6324258, Length=456, Percent_Identity=26.3157894736842, Blast_Score=132, Evalue=9e-32,
Organism=Drosophila melanogaster, GI24645909, Length=251, Percent_Identity=55.7768924302789, Blast_Score=293, Evalue=1e-79,
Organism=Drosophila melanogaster, GI18859875, Length=427, Percent_Identity=29.9765807962529, Blast_Score=152, Evalue=5e-37,
Organism=Drosophila melanogaster, GI20129315, Length=237, Percent_Identity=31.2236286919831, Blast_Score=119, Evalue=5e-27,
Organism=Drosophila melanogaster, GI24582497, Length=237, Percent_Identity=31.2236286919831, Blast_Score=118, Evalue=7e-27,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 43641; Mature: 43510

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTEIRVPTLGESVTEATVGKWFKKLGEAVAVDEPLIELETDKVTVEVPSPVAGKLSEII
CCCCEECCCCCCHHHHHHHHHHHHHHCCHHCCCCCCEEEECCEEEEECCCCHHHHHHHHH
AKEGDTVEVKALLGLVEAGAAGISQSFSPSATPIPEVPSELKQSSSSGAMQKDTMPPSPS
HCCCCCEEHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCC
AAKLMAENNIAKSNISGSGKRGQILKEDVLGVLEQEVKAPSVSAASSSASLVQEKHEERV
HHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
RMTKLRQTIARRLKDAQNTAAMLTTFNEVDMSAVMDLRKRYKDLFEKKHGVKLGFMGFFT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHH
KAVCHALKELPAVNAEIDGTDIVYKNYVNVGIAVGTDKGLVVPVVRHADQMSLAEIEKEI
HHHHHHHHHCCCCCCCCCCCCEEEECCEEEEEEEECCCCEEEHHHHCCCHHHHHHHHHHH
GRLGRLARDGKLAVSDMQGGTFTITNGGVYGSLMSTPILNAPQSGILGMHAIKERAMVVG
HHHHHHCCCCCEEEEECCCCEEEEECCCEEHHHHHCCCCCCCCCCCHHHHHHHHHHHHCC
GQIVIRPMMYLALSYDHRIVDGQEAVTFLVRVKESLEDPERLVLDL
CHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHCCC
>Mature Secondary Structure 
TTEIRVPTLGESVTEATVGKWFKKLGEAVAVDEPLIELETDKVTVEVPSPVAGKLSEII
CCCEECCCCCCHHHHHHHHHHHHHHCCHHCCCCCCEEEECCEEEEECCCCHHHHHHHHH
AKEGDTVEVKALLGLVEAGAAGISQSFSPSATPIPEVPSELKQSSSSGAMQKDTMPPSPS
HCCCCCEEHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCC
AAKLMAENNIAKSNISGSGKRGQILKEDVLGVLEQEVKAPSVSAASSSASLVQEKHEERV
HHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
RMTKLRQTIARRLKDAQNTAAMLTTFNEVDMSAVMDLRKRYKDLFEKKHGVKLGFMGFFT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHH
KAVCHALKELPAVNAEIDGTDIVYKNYVNVGIAVGTDKGLVVPVVRHADQMSLAEIEKEI
HHHHHHHHHCCCCCCCCCCCCEEEECCEEEEEEEECCCCEEEHHHHCCCHHHHHHHHHHH
GRLGRLARDGKLAVSDMQGGTFTITNGGVYGSLMSTPILNAPQSGILGMHAIKERAMVVG
HHHHHHCCCCCEEEEECCCCEEEEECCCEEHHHHHCCCCCCCCCCCHHHHHHHHHHHHCC
GQIVIRPMMYLALSYDHRIVDGQEAVTFLVRVKESLEDPERLVLDL
CHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12874367 [H]