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The map label for this gene is azo1 [H]

Identifier: 49476278

GI number: 49476278

Start: 1881425

End: 1881994

Strand: Reverse

Name: azo1 [H]

Synonym: BH16270

Alternate gene names: 49476278

Gene position: 1881994-1881425 (Counterclockwise)

Preceding gene: 49476279

Following gene: 49476277

Centisome position: 97.46

GC content: 42.46

Gene sequence:

>570_bases
TTGAATATTACGATTATTTTGGGGTCTGTACGTCAACCATCATTAACAAGAACTTTAGCCCATTGCTTGGCTGATTGCCT
CTTGATGCGGGATGCATCGCTTAATTGGGTTGATTTGCGTGAACAGCCCTTGCCGATTGTTGATCCAGATTATCATAGCC
ATGTAGAGGATAATCCAAGCGCTGCGGTACGTCAGTTTATTAAAAAAATCGCTGCGGCTGATGGTGTTATTCTTGCGAGT
CCTCTTTATCAAGGGTCTTATTCGGGTGTTTTAAAAAATGCTCTTGATCATTTGACCTATGATGCCTTTTTAAGAAAACC
GATTGGGCTTATCTCTCATGGTAGTACAGTCCAAAAATGTGCACAACCTTGTGAACATTTATTGCCGGTGGTGCGCACGC
TTTATGGTTATCCGCTGCAATGTCAAGTTACTTCGGCTAAGCAAGATTTTGCATCGGATGATGAAGGTCGTACATGGAAA
ATCATAAGTGAAGAGGTGCGGGAGCGTTGTGAGCGGCTTGCTGATGAAATGTGCGAATTTTTAAACCAGAAGAGAGAGAT
CATAGGATAA

Upstream 100 bases:

>100_bases
TGTCATCACAATTACTGTTATGATAGATGGCGTTTTTATATCAGTCATTTAGTTAGATTTGCCGGGAGAAAATATGGAGA
AAATATAAAGGAGAATTCTA

Downstream 100 bases:

>100_bases
AAAATTGAGCAATATCGTTATCGTTATTTAGTGGTAGGCGACACGGGAATACTTTCCCCTTTGGTTAATAGCTAGAGCTG
CAAGAAGTGATTGTTGTCAC

Product: hypothetical protein

Products: NAD(P)(+); FMNH(2). [C]

Alternate protein names: NADPH-dependent flavo-azoreductase; NADPH-flavin azoreductase [H]

Number of amino acids: Translated: 189; Mature: 189

Protein sequence:

>189_residues
MNITIILGSVRQPSLTRTLAHCLADCLLMRDASLNWVDLREQPLPIVDPDYHSHVEDNPSAAVRQFIKKIAAADGVILAS
PLYQGSYSGVLKNALDHLTYDAFLRKPIGLISHGSTVQKCAQPCEHLLPVVRTLYGYPLQCQVTSAKQDFASDDEGRTWK
IISEEVRERCERLADEMCEFLNQKREIIG

Sequences:

>Translated_189_residues
MNITIILGSVRQPSLTRTLAHCLADCLLMRDASLNWVDLREQPLPIVDPDYHSHVEDNPSAAVRQFIKKIAAADGVILAS
PLYQGSYSGVLKNALDHLTYDAFLRKPIGLISHGSTVQKCAQPCEHLLPVVRTLYGYPLQCQVTSAKQDFASDDEGRTWK
IISEEVRERCERLADEMCEFLNQKREIIG
>Mature_189_residues
MNITIILGSVRQPSLTRTLAHCLADCLLMRDASLNWVDLREQPLPIVDPDYHSHVEDNPSAAVRQFIKKIAAADGVILAS
PLYQGSYSGVLKNALDHLTYDAFLRKPIGLISHGSTVQKCAQPCEHLLPVVRTLYGYPLQCQVTSAKQDFASDDEGRTWK
IISEEVRERCERLADEMCEFLNQKREIIG

Specific function: Catalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Requires NADPH, but not NADH, as an electron donor for its activity [H]

COG id: COG0431

COG function: function code R; Predicted flavoprotein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the azoreductase type 2 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005025 [H]

Pfam domain/function: PF03358 FMN_red [H]

EC number: 1.5.1.29 [C]

Molecular weight: Translated: 21207; Mature: 21207

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
3.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNITIILGSVRQPSLTRTLAHCLADCLLMRDASLNWVDLREQPLPIVDPDYHSHVEDNPS
CEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCCHH
AAVRQFIKKIAAADGVILASPLYQGSYSGVLKNALDHLTYDAFLRKPIGLISHGSTVQKC
HHHHHHHHHHHHCCCEEEECCHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHH
AQPCEHLLPVVRTLYGYPLQCQVTSAKQDFASDDEGRTWKIISEEVRERCERLADEMCEF
HHHHHHHHHHHHHHHCCCCEEEECCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LNQKREIIG
HHHHHHHCC
>Mature Secondary Structure
MNITIILGSVRQPSLTRTLAHCLADCLLMRDASLNWVDLREQPLPIVDPDYHSHVEDNPS
CEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCCHH
AAVRQFIKKIAAADGVILASPLYQGSYSGVLKNALDHLTYDAFLRKPIGLISHGSTVQKC
HHHHHHHHHHHHCCCEEEECCHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHH
AQPCEHLLPVVRTLYGYPLQCQVTSAKQDFASDDEGRTWKIISEEVRERCERLADEMCEF
HHHHHHHHHHHHHHHCCCCEEEECCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LNQKREIIG
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD(P)H; FMN [C]

Specific reaction: NAD(P)H + FMN = NAD(P)(+) + FMNH(2). [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA