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The map label for this gene is thiD2 [H]

Identifier: 49476279

GI number: 49476279

Start: 1882671

End: 1883534

Strand: Reverse

Name: thiD2 [H]

Synonym: BH16280

Alternate gene names: 49476279

Gene position: 1883534-1882671 (Counterclockwise)

Preceding gene: 49476284

Following gene: 49476278

Centisome position: 97.54

GC content: 41.55

Gene sequence:

>864_bases
ATGAAATTACAGGTTAGCCAAGACACTCTTTTTATTCCACGGATTTTATCTATTGCGGGAACAGATCCTTCTGGTGGCGC
TGGAATGCAAGCGGATTTGAAAGTTTTTTCAGCTATGAAAACCTATGGAATGAGTGTGGTGACCGCTGTGGTTGCACAAA
ATACTCAAGGTGTACGTGCTTTTCAGGCGTTGGATGCTTCTTTTGTTGCTGACCAAATTGATGCGGTTTTTGAAGATATT
CATGTTGATGCTGTTAAAATTGGTATGGTAGCAAATGCTCAAATTGCTCGGACTGTCGCAGAACGTCTTGCTTATCACAA
AGCGCGCTTCATTGTTTTTGATCCCGTTATGGTGGCAAAAAGTGGCGATGTCCTTTTAAAGTCTGATGCGATTGAAATTA
TGCGTGATGTTCTTGTGCCGATGTCAACTTTGATCACGCCAAATTTACCTGAGGCATCTCTATTGTTGGGATGTGAGGTA
AAGTGGTCTTTAGACGCTATGTACCAATATGGTCCACAGCTTCTGACTCTAGGGTGTCATGCAGTTCTTTTGAAGGGTGG
GCATTTGGACGTTTTTTCTCGTACAAATGAGGCGAATTATGATTATTCTAGTCCAGATCTTTATTGTGATTGTGAAGATC
TTGTCATGCTTGAAGCCCCTCGTTTTATAACCACTCATGATCATGGTACAGGTTGTACTATTTCAGCTGCCATCGCAGCT
TTGTTGCCTACAAAGCCTTTGGTTGATGCGGTTAAACAAGCAAAAGACTATTTAAATGGGGCTTTATCTACCTCAAGTGC
TTTACAAGTAGGGAAAGGACGTGGTCCCCTTCACCATTTTTATGAATTATGGAAGAATGTATGA

Upstream 100 bases:

>100_bases
TTACTTTGACACTGGAAAACACCTCTCTTTTATGTTTTTTTTAATATCTAATTTGGGGATGGTTTCTTTTTTGATGTTTA
GAATATAAGGAAAAGGGTGG

Downstream 100 bases:

>100_bases
AACAAATAATTGTAAAGCAAAAATTTTCTGCTCTGTTGTATGTTTCGTTGTTATACTTTCCATGGTTATACAAAAACTAA
TCAAAATAAGTGTCGTTTGA

Product: phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MKLQVSQDTLFIPRILSIAGTDPSGGAGMQADLKVFSAMKTYGMSVVTAVVAQNTQGVRAFQALDASFVADQIDAVFEDI
HVDAVKIGMVANAQIARTVAERLAYHKARFIVFDPVMVAKSGDVLLKSDAIEIMRDVLVPMSTLITPNLPEASLLLGCEV
KWSLDAMYQYGPQLLTLGCHAVLLKGGHLDVFSRTNEANYDYSSPDLYCDCEDLVMLEAPRFITTHDHGTGCTISAAIAA
LLPTKPLVDAVKQAKDYLNGALSTSSALQVGKGRGPLHHFYELWKNV

Sequences:

>Translated_287_residues
MKLQVSQDTLFIPRILSIAGTDPSGGAGMQADLKVFSAMKTYGMSVVTAVVAQNTQGVRAFQALDASFVADQIDAVFEDI
HVDAVKIGMVANAQIARTVAERLAYHKARFIVFDPVMVAKSGDVLLKSDAIEIMRDVLVPMSTLITPNLPEASLLLGCEV
KWSLDAMYQYGPQLLTLGCHAVLLKGGHLDVFSRTNEANYDYSSPDLYCDCEDLVMLEAPRFITTHDHGTGCTISAAIAA
LLPTKPLVDAVKQAKDYLNGALSTSSALQVGKGRGPLHHFYELWKNV
>Mature_287_residues
MKLQVSQDTLFIPRILSIAGTDPSGGAGMQADLKVFSAMKTYGMSVVTAVVAQNTQGVRAFQALDASFVADQIDAVFEDI
HVDAVKIGMVANAQIARTVAERLAYHKARFIVFDPVMVAKSGDVLLKSDAIEIMRDVLVPMSTLITPNLPEASLLLGCEV
KWSLDAMYQYGPQLLTLGCHAVLLKGGHLDVFSRTNEANYDYSSPDLYCDCEDLVMLEAPRFITTHDHGTGCTISAAIAA
LLPTKPLVDAVKQAKDYLNGALSTSSALQVGKGRGPLHHFYELWKNV

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P. Shows no activity with pyridoxal, pyridoxamine or pyridoxine [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=270, Percent_Identity=49.2592592592593, Blast_Score=256, Evalue=9e-70,
Organism=Saccharomyces cerevisiae, GI6324997, Length=277, Percent_Identity=33.2129963898917, Blast_Score=114, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6324517, Length=277, Percent_Identity=32.4909747292419, Blast_Score=114, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6325378, Length=280, Percent_Identity=28.9285714285714, Blast_Score=110, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013749
- InterPro:   IPR004399 [H]

Pfam domain/function: PF08543 Phos_pyr_kin [H]

EC number: =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 31033; Mature: 31033

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLQVSQDTLFIPRILSIAGTDPSGGAGMQADLKVFSAMKTYGMSVVTAVVAQNTQGVRA
CEEECCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
FQALDASFVADQIDAVFEDIHVDAVKIGMVANAQIARTVAERLAYHKARFIVFDPVMVAK
HHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHCEEEEECCEEEEC
SGDVLLKSDAIEIMRDVLVPMSTLITPNLPEASLLLGCEVKWSLDAMYQYGPQLLTLGCH
CCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECEEEECHHHHHHCCCHHHHHHHE
AVLLKGGHLDVFSRTNEANYDYSSPDLYCDCEDLVMLEAPRFITTHDHGTGCTISAAIAA
EEEEECCCEEEEECCCCCCCCCCCCCEEECCCHHEEECCCCEEEECCCCCCCHHHHHHHH
LLPTKPLVDAVKQAKDYLNGALSTSSALQVGKGRGPLHHFYELWKNV
HCCCCHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MKLQVSQDTLFIPRILSIAGTDPSGGAGMQADLKVFSAMKTYGMSVVTAVVAQNTQGVRA
CEEECCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
FQALDASFVADQIDAVFEDIHVDAVKIGMVANAQIARTVAERLAYHKARFIVFDPVMVAK
HHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHCEEEEECCEEEEC
SGDVLLKSDAIEIMRDVLVPMSTLITPNLPEASLLLGCEVKWSLDAMYQYGPQLLTLGCH
CCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECEEEECHHHHHHCCCHHHHHHHE
AVLLKGGHLDVFSRTNEANYDYSSPDLYCDCEDLVMLEAPRFITTHDHGTGCTISAAIAA
EEEEECCCEEEEECCCCCCCCCCCCCEEECCCHHEEECCCCEEEECCCCCCCHHHHHHHH
LLPTKPLVDAVKQAKDYLNGALSTSSALQVGKGRGPLHHFYELWKNV
HCCCCHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10075431; 9097040; 9278503 [H]