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The map label for this gene is ruvB [H]

Identifier: 49476146

GI number: 49476146

Start: 1709079

End: 1710164

Strand: Reverse

Name: ruvB [H]

Synonym: BH14890

Alternate gene names: 49476146

Gene position: 1710164-1709079 (Counterclockwise)

Preceding gene: 49476147

Following gene: 49476145

Centisome position: 88.56

GC content: 44.75

Gene sequence:

>1086_bases
ATGCATAAAGATGAAGATCAACGCCTTTTGGGTGCCGTCCCCCTTCCTAATGATCCAGATCGTTCCTTAAGACCACAAGT
GCTTGATGACTTTATTGGTCAAGAGGCAGCACGGGCCAATTTAAAAATATTTATTGAAGCGGCAAAAGCGCGTCAAGAGG
CCCTCGATCATGTTTTGTTTGTAGGACCACCTGGTTTAGGCAAAACAACGCTTTCACAAATTATGGCTAAGGAACTAGGG
GTTAATTTTCGCTCTACCTCAGGTCCTGTTATTGCTAAGGCGGGAGATTTGGCAGCTCTCTTAACCAATCTAGAAGAGCG
CGATGTTTTGTTTATTGATGAAATTCATCGTTTAAGTCCGGCTATTGAAGAAATTCTTTATCCCGCCATGGAAGATTATC
AGCTTGATCTTATTATTGGTGAGGGACCGGCAGCACGTTCGGTTAAAATTGATCTTGCTAAATTTACTTTGGTTGCGGCA
ACCACGCGTTTAGGGCTTTTGACCACACCATTACGAGACCGTTTCGGTATCCCCATTCGATTGAATTTCTATACCATAGA
AGAATTGGAATATATCGTTCAGCGTAATGCACGGCTTTTTTCGGTACAGATCAGTGAGGATGGTGCTCATGAAATTGCTC
GTCGAGCACGCGGTACTCCACGGATTGCCGGGCGACTTTTACGGCGGGTTTGTGATTTTGCTCTTGTCAAAAGGGCAAAA
AAAATTGATCGAAAAATTGCTGATGAAGCACTGTCTCGTCTAGAAGTTGATCATCTAGGGCTCGATCCACTTGACCGTCG
TTATTTGTTGCTGATTGCTCAGACTTTTTTAGGTGGTCCAGTGGGTATTGAAACCATTGCTGCTGCTCTTTCAGAACCGC
GGGATGCTATTGAAGATATTATTGAACCCTATCTTCTCCAACAAGGTTTTATTCAGCGAACTGCCCGCGGGCGCATTTTG
CACCAAAAAGCTTGGAGTCATTTGGGGCTTTGTGCCCCGGCTTCCACACAAAAACATCCTCAATTGTTCGATATGCCAGA
CAATGCATCCCGCCAGACGGTTTTATGGGATGAGGCTGATGATTGA

Upstream 100 bases:

>100_bases
TTTAGCGGTGAACGCTCTTGAAGGGGAGACTGTTTCCTCTGCATTGCTTATTCGCCATAGCCTCAAATTGCTTTCTCCTC
CTACTTAGCGCAAGGTCACA

Downstream 100 bases:

>100_bases
TTTCCTTTTTCAAGAGATGCTATGCAGATGTTTTGCATGGATGATATATCATGAATTATAAGCCTATTTGCCCGTAGGGG
AATTCTTCCCCAAAAAGCTT

Product: Holliday junction DNA helicase RuvB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MHKDEDQRLLGAVPLPNDPDRSLRPQVLDDFIGQEAARANLKIFIEAAKARQEALDHVLFVGPPGLGKTTLSQIMAKELG
VNFRSTSGPVIAKAGDLAALLTNLEERDVLFIDEIHRLSPAIEEILYPAMEDYQLDLIIGEGPAARSVKIDLAKFTLVAA
TTRLGLLTTPLRDRFGIPIRLNFYTIEELEYIVQRNARLFSVQISEDGAHEIARRARGTPRIAGRLLRRVCDFALVKRAK
KIDRKIADEALSRLEVDHLGLDPLDRRYLLLIAQTFLGGPVGIETIAAALSEPRDAIEDIIEPYLLQQGFIQRTARGRIL
HQKAWSHLGLCAPASTQKHPQLFDMPDNASRQTVLWDEADD

Sequences:

>Translated_361_residues
MHKDEDQRLLGAVPLPNDPDRSLRPQVLDDFIGQEAARANLKIFIEAAKARQEALDHVLFVGPPGLGKTTLSQIMAKELG
VNFRSTSGPVIAKAGDLAALLTNLEERDVLFIDEIHRLSPAIEEILYPAMEDYQLDLIIGEGPAARSVKIDLAKFTLVAA
TTRLGLLTTPLRDRFGIPIRLNFYTIEELEYIVQRNARLFSVQISEDGAHEIARRARGTPRIAGRLLRRVCDFALVKRAK
KIDRKIADEALSRLEVDHLGLDPLDRRYLLLIAQTFLGGPVGIETIAAALSEPRDAIEDIIEPYLLQQGFIQRTARGRIL
HQKAWSHLGLCAPASTQKHPQLFDMPDNASRQTVLWDEADD
>Mature_361_residues
MHKDEDQRLLGAVPLPNDPDRSLRPQVLDDFIGQEAARANLKIFIEAAKARQEALDHVLFVGPPGLGKTTLSQIMAKELG
VNFRSTSGPVIAKAGDLAALLTNLEERDVLFIDEIHRLSPAIEEILYPAMEDYQLDLIIGEGPAARSVKIDLAKFTLVAA
TTRLGLLTTPLRDRFGIPIRLNFYTIEELEYIVQRNARLFSVQISEDGAHEIARRARGTPRIAGRLLRRVCDFALVKRAK
KIDRKIADEALSRLEVDHLGLDPLDRRYLLLIAQTFLGGPVGIETIAAALSEPRDAIEDIIEPYLLQQGFIQRTARGRIL
HQKAWSHLGLCAPASTQKHPQLFDMPDNASRQTVLWDEADD

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG2255

COG function: function code L; Holliday junction resolvasome, helicase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvB family [H]

Homologues:

Organism=Escherichia coli, GI1788167, Length=331, Percent_Identity=61.3293051359517, Blast_Score=414, Evalue=1e-117,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR004605
- InterPro:   IPR008823
- InterPro:   IPR008824
- InterPro:   IPR011991
- ProDom:   PD005323 [H]

Pfam domain/function: PF05491 RuvB_C; PF05496 RuvB_N [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 40277; Mature: 40277

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHKDEDQRLLGAVPLPNDPDRSLRPQVLDDFIGQEAARANLKIFIEAAKARQEALDHVLF
CCCCCCCHHEECCCCCCCCCCCCCHHHHHHHHCCHHHHCCEEEEEEHHHHHHHHHHCEEE
VGPPGLGKTTLSQIMAKELGVNFRSTSGPVIAKAGDLAALLTNLEERDVLFIDEIHRLSP
ECCCCCCHHHHHHHHHHHHCCCEECCCCCEEECCCHHHHHHHCCCCCCEEHHHHHHHHHH
AIEEILYPAMEDYQLDLIIGEGPAARSVKIDLAKFTLVAATTRLGLLTTPLRDRFGIPIR
HHHHHHHCCCCCCEEEEEECCCCCCCEEEEEHHHHHHHHHHHHHHHEECCHHHHCCCCEE
LNFYTIEELEYIVQRNARLFSVQISEDGAHEIARRARGTPRIAGRLLRRVCDFALVKRAK
EEEEEHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
KIDRKIADEALSRLEVDHLGLDPLDRRYLLLIAQTFLGGPVGIETIAAALSEPRDAIEDI
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCHHHHHHHH
IEPYLLQQGFIQRTARGRILHQKAWSHLGLCAPASTQKHPQLFDMPDNASRQTVLWDEAD
HHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCCCCEEECCCCC
D
C
>Mature Secondary Structure
MHKDEDQRLLGAVPLPNDPDRSLRPQVLDDFIGQEAARANLKIFIEAAKARQEALDHVLF
CCCCCCCHHEECCCCCCCCCCCCCHHHHHHHHCCHHHHCCEEEEEEHHHHHHHHHHCEEE
VGPPGLGKTTLSQIMAKELGVNFRSTSGPVIAKAGDLAALLTNLEERDVLFIDEIHRLSP
ECCCCCCHHHHHHHHHHHHCCCEECCCCCEEECCCHHHHHHHCCCCCCEEHHHHHHHHHH
AIEEILYPAMEDYQLDLIIGEGPAARSVKIDLAKFTLVAATTRLGLLTTPLRDRFGIPIR
HHHHHHHCCCCCCEEEEEECCCCCCCEEEEEHHHHHHHHHHHHHHHEECCHHHHCCCCEE
LNFYTIEELEYIVQRNARLFSVQISEDGAHEIARRARGTPRIAGRLLRRVCDFALVKRAK
EEEEEHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
KIDRKIADEALSRLEVDHLGLDPLDRRYLLLIAQTFLGGPVGIETIAAALSEPRDAIEDI
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCHHHHHHHH
IEPYLLQQGFIQRTARGRILHQKAWSHLGLCAPASTQKHPQLFDMPDNASRQTVLWDEAD
HHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCCCCEEECCCCC
D
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA