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The map label for this gene is gcvH

Identifier: 49475980

GI number: 49475980

Start: 1434244

End: 1434612

Strand: Reverse

Name: gcvH

Synonym: BH12830

Alternate gene names: 49475980

Gene position: 1434612-1434244 (Counterclockwise)

Preceding gene: 49475981

Following gene: 49475979

Centisome position: 74.29

GC content: 40.92

Gene sequence:

>369_bases
ATGTCTAAAACTTATTTTACACAAGATCATGAATGGCTTAGCGTTGAAGGACAAGTGGTTACTGTTGGGATCACAGATTA
TGCTCAAGAGCAATTAGGGGATTTGGTTTTTATTGATTTGCCACAAAATGGAACAAAACTTTCTAAGGGAGATGCTGCTG
CTGTTGTTGAATCGGTGAAAGCGGCTTCAGATGTTTATGCACCTCTTGATGGTGAAGTGGTCGAAATAAATGCAGCTTTG
GCAGAGAGTCCTGAACTGGTAAACCAAAAAGCTGAAACAGAAGGTTGGCTCTGGAAAATGACTGTACAGGATGAAACGCA
ACTTGAAAGGCTGCTGGATGAAGCTGCTTATAAAGAACTGATTGGATGA

Upstream 100 bases:

>100_bases
GGAACAGAAGTATTTACAGAGCTGCGTGGCAAAAAGATTGCACTGTCTGTTCATTCACTCCCTTTTGTTGAACAACGTTA
TTTTAAAGGATAATTTTTTT

Downstream 100 bases:

>100_bases
GTCCTATGTTAGAGCGTCCTTTTTTTTCTCGCCATATTGGGCTTCGTCCTGATGAAAAACAAAAAATGCTTGATATTGTA
GCAGTCGATTCTATTGATAC

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 122; Mature: 121

Protein sequence:

>122_residues
MSKTYFTQDHEWLSVEGQVVTVGITDYAQEQLGDLVFIDLPQNGTKLSKGDAAAVVESVKAASDVYAPLDGEVVEINAAL
AESPELVNQKAETEGWLWKMTVQDETQLERLLDEAAYKELIG

Sequences:

>Translated_122_residues
MSKTYFTQDHEWLSVEGQVVTVGITDYAQEQLGDLVFIDLPQNGTKLSKGDAAAVVESVKAASDVYAPLDGEVVEINAAL
AESPELVNQKAETEGWLWKMTVQDETQLERLLDEAAYKELIG
>Mature_121_residues
SKTYFTQDHEWLSVEGQVVTVGITDYAQEQLGDLVFIDLPQNGTKLSKGDAAAVVESVKAASDVYAPLDGEVVEINAALA
ESPELVNQKAETEGWLWKMTVQDETQLERLLDEAAYKELIG

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI89057342, Length=121, Percent_Identity=51.2396694214876, Blast_Score=132, Evalue=8e-32,
Organism=Homo sapiens, GI49574537, Length=121, Percent_Identity=51.2396694214876, Blast_Score=132, Evalue=8e-32,
Organism=Escherichia coli, GI1789271, Length=117, Percent_Identity=53.8461538461538, Blast_Score=132, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI17507493, Length=119, Percent_Identity=51.2605042016807, Blast_Score=127, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17551294, Length=115, Percent_Identity=46.9565217391304, Blast_Score=121, Evalue=7e-29,
Organism=Saccharomyces cerevisiae, GI6319272, Length=107, Percent_Identity=42.0560747663551, Blast_Score=96, Evalue=2e-21,
Organism=Drosophila melanogaster, GI17865652, Length=117, Percent_Identity=45.2991452991453, Blast_Score=110, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GCSH_BARHE (Q6G2F0)

Other databases:

- EMBL:   BX897699
- RefSeq:   YP_034021.1
- PDB:   3MXU
- PDBsum:   3MXU
- ProteinModelPortal:   Q6G2F0
- SMR:   Q6G2F0
- GeneID:   2864734
- GenomeReviews:   BX897699_GR
- KEGG:   bhe:BH12830
- NMPDR:   fig|283166.1.peg.1149
- HOGENOM:   HBG693789
- OMA:   TSDHEWL
- PhylomeDB:   Q6G2F0
- ProtClustDB:   PRK01202
- BioCyc:   BHEN283166:BH12830-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_00272
- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053
- PANTHER:   PTHR11715
- TIGRFAMs:   TIGR00527

Pfam domain/function: PF01597 GCV_H; SSF51230 Hybrid_motif

EC number: NA

Molecular weight: Translated: 13403; Mature: 13272

Theoretical pI: Translated: 3.86; Mature: 3.86

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKTYFTQDHEWLSVEGQVVTVGITDYAQEQLGDLVFIDLPQNGTKLSKGDAAAVVESVK
CCCCCCCCCCCEEEECCEEEEEECCHHHHHHHCCEEEEECCCCCCCCCCCHHHHHHHHHH
AASDVYAPLDGEVVEINAALAESPELVNQKAETEGWLWKMTVQDETQLERLLDEAAYKEL
HHHHCCCCCCCCEEEEEEHHCCCHHHHCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHH
IG
CC
>Mature Secondary Structure 
SKTYFTQDHEWLSVEGQVVTVGITDYAQEQLGDLVFIDLPQNGTKLSKGDAAAVVESVK
CCCCCCCCCCEEEECCEEEEEECCHHHHHHHCCEEEEECCCCCCCCCCCHHHHHHHHHH
AASDVYAPLDGEVVEINAALAESPELVNQKAETEGWLWKMTVQDETQLERLLDEAAYKEL
HHHHCCCCCCCCEEEEEEHHCCCHHHHCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHH
IG
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA