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The map label for this gene is miaA

Identifier: 49475827

GI number: 49475827

Start: 1203067

End: 1203939

Strand: Reverse

Name: miaA

Synonym: BH10930

Alternate gene names: 49475827

Gene position: 1203939-1203067 (Counterclockwise)

Preceding gene: 49475828

Following gene: 49475826

Centisome position: 62.35

GC content: 40.09

Gene sequence:

>873_bases
ATGAAAGGGAGAACGATTACCTTGATAGCAGGGCCAACTGCGAGTGGCAAATCAGCGCTTGCCTTACAAATAGCTCAGGA
AAAAAATGCTCTCATTATTAATACTGATTCAATGCAGGTTTATGATGTTTTAAATATTTTAACGGCAAGGCCTACACGGA
CTGATACTGCAACTGTTCCGCACTATCTTTATGGTTATGTGAATCCTGCTCTTCACTACTCAGTGGGACAGTGGTTATGT
GATGTTAGCAAGCTTTTAATGACATTTACGTCGAAATCACTTATTTTTGTTGGTGGAACAGGACTTTATTTTCGTGCTCT
TTTAGAGGGAATTTCAAAAATCCCTGATATTCCAGATGTTGTGCGGCAGAAATGGCGACTTCGGCTTGATAAAGAGGGAG
CTGAGAACCTTTATCGCCAATTGTGGCAGGTTGATGCTGTTCTCGCTGAGAAAATTTCTTCACAAGATGGACAACGTATT
GTTCGTGCTTTGGAAGTTTATGATGCAACTGATAAAAAGCTAAGTTGGTGGCAGAAGAAAAAAACAACACCTCTTATTGC
GCGAAATTGTTCAGAAAAACTTCTTCTCATTCCACCGCGTCAGTTGCTTTATGAACGTATTCATAAACGGTTGGACTCTA
TGATTGAAAAAGGCGCTTTAGAGGAGGTGATTGCTATGAAAAAGCTTGCGCTTTCTCCTTTGTTACCGGCGATGAAGGCT
ATTGGTATACCTGAATTTATAGCTTATTTGGATGGGAAGCAAAGTTTTGAGGAGGCTCTTGAAATGGTGAAGACACAAAC
GAGGAGGTATGCAAAAAGGCAAATAACATGGTTTCGTAATCAATTTGATGAAGAATGGATGCTTCTTTCTTGA

Upstream 100 bases:

>100_bases
CATTCACATTTATGACGATGGAGATGAAATACAAGGTCTTTGGAGTTGCTCGTGCGTGTTGCACGAAGTTGTTGAGTGAT
GGATGTAAGGATTTCCATTT

Downstream 100 bases:

>100_bases
TAAAAGAGTCAAGAATCCTAAAGGATAATGTAAAAAACTTAATTATGCATGGAATTTTTTAAGAAATATAATTTTTTATT
GACGGATGGTATCAATGGAA

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MKGRTITLIAGPTASGKSALALQIAQEKNALIINTDSMQVYDVLNILTARPTRTDTATVPHYLYGYVNPALHYSVGQWLC
DVSKLLMTFTSKSLIFVGGTGLYFRALLEGISKIPDIPDVVRQKWRLRLDKEGAENLYRQLWQVDAVLAEKISSQDGQRI
VRALEVYDATDKKLSWWQKKKTTPLIARNCSEKLLLIPPRQLLYERIHKRLDSMIEKGALEEVIAMKKLALSPLLPAMKA
IGIPEFIAYLDGKQSFEEALEMVKTQTRRYAKRQITWFRNQFDEEWMLLS

Sequences:

>Translated_290_residues
MKGRTITLIAGPTASGKSALALQIAQEKNALIINTDSMQVYDVLNILTARPTRTDTATVPHYLYGYVNPALHYSVGQWLC
DVSKLLMTFTSKSLIFVGGTGLYFRALLEGISKIPDIPDVVRQKWRLRLDKEGAENLYRQLWQVDAVLAEKISSQDGQRI
VRALEVYDATDKKLSWWQKKKTTPLIARNCSEKLLLIPPRQLLYERIHKRLDSMIEKGALEEVIAMKKLALSPLLPAMKA
IGIPEFIAYLDGKQSFEEALEMVKTQTRRYAKRQITWFRNQFDEEWMLLS
>Mature_290_residues
MKGRTITLIAGPTASGKSALALQIAQEKNALIINTDSMQVYDVLNILTARPTRTDTATVPHYLYGYVNPALHYSVGQWLC
DVSKLLMTFTSKSLIFVGGTGLYFRALLEGISKIPDIPDVVRQKWRLRLDKEGAENLYRQLWQVDAVLAEKISSQDGQRI
VRALEVYDATDKKLSWWQKKKTTPLIARNCSEKLLLIPPRQLLYERIHKRLDSMIEKGALEEVIAMKKLALSPLLPAMKA
IGIPEFIAYLDGKQSFEEALEMVKTQTRRYAKRQITWFRNQFDEEWMLLS

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=317, Percent_Identity=27.4447949526814, Blast_Score=109, Evalue=3e-24,
Organism=Escherichia coli, GI1790613, Length=283, Percent_Identity=33.9222614840989, Blast_Score=150, Evalue=9e-38,
Organism=Caenorhabditis elegans, GI25144712, Length=305, Percent_Identity=25.9016393442623, Blast_Score=78, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6324848, Length=294, Percent_Identity=29.5918367346939, Blast_Score=119, Evalue=8e-28,
Organism=Drosophila melanogaster, GI24649830, Length=333, Percent_Identity=28.5285285285285, Blast_Score=107, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_BARHE (Q6G2T3)

Other databases:

- EMBL:   BX897699
- RefSeq:   YP_033868.1
- GeneID:   2865621
- GenomeReviews:   BX897699_GR
- KEGG:   bhe:BH10930
- NMPDR:   fig|283166.1.peg.996
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- ProtClustDB:   PRK00091
- BioCyc:   BHEN283166:BH10930-MONOMER
- BRENDA:   2.5.1.8
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 33147; Mature: 33147

Theoretical pI: Translated: 9.92; Mature: 9.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGRTITLIAGPTASGKSALALQIAQEKNALIINTDSMQVYDVLNILTARPTRTDTATVP
CCCCEEEEEECCCCCCCCCEEEEEECCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCCH
HYLYGYVNPALHYSVGQWLCDVSKLLMTFTSKSLIFVGGTGLYFRALLEGISKIPDIPDV
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHCCCCHHH
VRQKWRLRLDKEGAENLYRQLWQVDAVLAEKISSQDGQRIVRALEVYDATDKKLSWWQKK
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHH
KTTPLIARNCSEKLLLIPPRQLLYERIHKRLDSMIEKGALEEVIAMKKLALSPLLPAMKA
CCCHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGIPEFIAYLDGKQSFEEALEMVKTQTRRYAKRQITWFRNQFDEEWMLLS
CCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECC
>Mature Secondary Structure
MKGRTITLIAGPTASGKSALALQIAQEKNALIINTDSMQVYDVLNILTARPTRTDTATVP
CCCCEEEEEECCCCCCCCCEEEEEECCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCCH
HYLYGYVNPALHYSVGQWLCDVSKLLMTFTSKSLIFVGGTGLYFRALLEGISKIPDIPDV
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHCCCCHHH
VRQKWRLRLDKEGAENLYRQLWQVDAVLAEKISSQDGQRIVRALEVYDATDKKLSWWQKK
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHH
KTTPLIARNCSEKLLLIPPRQLLYERIHKRLDSMIEKGALEEVIAMKKLALSPLLPAMKA
CCCHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGIPEFIAYLDGKQSFEEALEMVKTQTRRYAKRQITWFRNQFDEEWMLLS
CCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA