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The map label for this gene is ilvB [H]

Identifier: 49475826

GI number: 49475826

Start: 1200996

End: 1202795

Strand: Reverse

Name: ilvB [H]

Synonym: BH10920

Alternate gene names: 49475826

Gene position: 1202795-1200996 (Counterclockwise)

Preceding gene: 49475827

Following gene: 49475825

Centisome position: 62.29

GC content: 41.28

Gene sequence:

>1800_bases
ATGACAAATCATCCAAGAATGCAAGAGAGTGCGAACGGAAAAAAAATGTTGGGCGCTGAAATGGTGGTTCAAGCTCTTAT
TGATCAAGGGGTTAAACATCTATTTGGTTATCCTGGTGGGGCTGTCCTTCCTATTTTTGATGTACTTTATCAACAGGATT
CACTGCAGCATATTTTGGTGCGTCATGAACAAGCAGCAGGTCATGCTGCTGAAGGTTATGCACGCAGCACAGGGAAGGTC
GGCGTTATGCTTGTGACTTCTGGTCCAGGGGCAACGAACGCTGTAACACCTTTGCAAGATGCACTTATGGATTCTATTCC
TCTTGTTTGTTTTTCTGGTCAGGTGTCAACAACTGTGATTGGAACAAATGGTTTCCAAGAGGCTGATACAGTTGGTATTA
CAAAGTCCTGTACGAAACAAAATTGGTTGGTTAAAGATGTAAATGATCTTGCCGCTATCATTCATGAAGCTTTTGCTGTT
GCTCAATCAGGACGTCCAGGGCCAGTTTTGGTTGATATTCCTAAGGATATACAATTAGCTTCAGGTGTTTATAGAGCGCC
TCAAGTTTTGGTATCTAGGCATTTTTCTCCACAGTTGGAAAGGAATATTGAGGCTATTGAATGTGCTGTTTCTCTTTTAG
AAGAAGCAAAACGTCCTGTTATTTATTCTGGTGGTGGTGTTATTTCATCAGGTCTTCAAGCTGTACAGCTTTTGCGTGAT
TTGGTTCAATTGGGAGATTTTCCGATTACTTCAACACTCATGGGACTTGGTGCTTATCCGGCTTCTGGTAAAAATTGGCT
TGGAATGCTTGGAATGCATGGAACTTATGAAGCTAATATGGCCATGCATGATTGTGATGTTATGCTGGCTGTTGGGGCAC
GGTTTGATGATCGTATCACTGGGCGGCTTGATGCATTTGCACCTCATGCGCGCATTATTCATATTGATGTTGATCCTTCT
TCTATCAATAAGATCGTAAAAGTGGAAGTTCCAATTATTGGCGATGTGGCTGATGTTTTAGGAGATATGATGCAATGTTT
GCATAGACAGCAACCAATCTTTAAGAGTGAAGGCCGTGATGCGTGGTGGAAGCAAATTGATTGCTGGAGAGCTCGTAATT
CATTAGCATACCCTAAGAAAAAAGATATTATTATGCCACAATACGCTCTTGAACGACTTTATGCTCTTACTAAAGATGTG
AATGCTTATATTACGACTGATGTTGGGCAGCATCAGATGTGGGCTGCACAATATTATCACTTTGATGAGCCAAACCATTG
GATGACTTCTGGTGGTCTTGGAACTATGGGTTATGGTTTGCCTGCTGCTATTGGGGTACAAATCGCACATCCTGATGCAT
TTGTTGTATGTATTTCTGGTGATGCTTCAATTCAAATGAATATCCAAGAACTCGCAACGGCAATGCAGTATAACACACCA
GTGAAAATTTTTATTTTGAATAATCAGTATATGGGTATGGTCCGCCAGTGGCAGCAGTTGTTGCATGGTAATCGTCTTTC
TCATTCTTACACCGAATCAATGCCTGACTTTGTGAAATTGGCACAAGCTTATGGAGGGATTGGTATTCATTGTTCCAAAC
CGGATGAACTTGATGATAAAATACAGCAAATGATTGACTGTGACAAACCAGTTCTCTTCGATTGCCGGGTTGATAATTTA
GAAAATTGTTTTCCAATGATCCCATCTGGGTGTGCACATAATGATATGCTATTACCTGACGAGGCTCATGATGAAGCAGT
TGCCAATGCTATTTCCGCTGAAGGACAATGTTTGGTTTAA

Upstream 100 bases:

>100_bases
ACAATCTATGTTTTGTGTGTAGTAGGCTCTCTAAGAGCCTTTTTTTATGGGCTCTTCTGGGGTCTTTTTTTATTGTGCTA
TCAAGTAGACGAGTGGGAAA

Downstream 100 bases:

>100_bases
GGAGTTTGCAATTACGATAACACGGATTTAAACTTTTGCTTTAGTGACTTTTTTTACTTTGAATATGATTCAACAGAAGC
TCAATATGTAGGTTGTTTTA

Product: acetolactate synthase 3 catalytic subunit

Products: NA

Alternate protein names: AHAS-III; ALS-III; Acetohydroxy-acid synthase III large subunit [H]

Number of amino acids: Translated: 599; Mature: 598

Protein sequence:

>599_residues
MTNHPRMQESANGKKMLGAEMVVQALIDQGVKHLFGYPGGAVLPIFDVLYQQDSLQHILVRHEQAAGHAAEGYARSTGKV
GVMLVTSGPGATNAVTPLQDALMDSIPLVCFSGQVSTTVIGTNGFQEADTVGITKSCTKQNWLVKDVNDLAAIIHEAFAV
AQSGRPGPVLVDIPKDIQLASGVYRAPQVLVSRHFSPQLERNIEAIECAVSLLEEAKRPVIYSGGGVISSGLQAVQLLRD
LVQLGDFPITSTLMGLGAYPASGKNWLGMLGMHGTYEANMAMHDCDVMLAVGARFDDRITGRLDAFAPHARIIHIDVDPS
SINKIVKVEVPIIGDVADVLGDMMQCLHRQQPIFKSEGRDAWWKQIDCWRARNSLAYPKKKDIIMPQYALERLYALTKDV
NAYITTDVGQHQMWAAQYYHFDEPNHWMTSGGLGTMGYGLPAAIGVQIAHPDAFVVCISGDASIQMNIQELATAMQYNTP
VKIFILNNQYMGMVRQWQQLLHGNRLSHSYTESMPDFVKLAQAYGGIGIHCSKPDELDDKIQQMIDCDKPVLFDCRVDNL
ENCFPMIPSGCAHNDMLLPDEAHDEAVANAISAEGQCLV

Sequences:

>Translated_599_residues
MTNHPRMQESANGKKMLGAEMVVQALIDQGVKHLFGYPGGAVLPIFDVLYQQDSLQHILVRHEQAAGHAAEGYARSTGKV
GVMLVTSGPGATNAVTPLQDALMDSIPLVCFSGQVSTTVIGTNGFQEADTVGITKSCTKQNWLVKDVNDLAAIIHEAFAV
AQSGRPGPVLVDIPKDIQLASGVYRAPQVLVSRHFSPQLERNIEAIECAVSLLEEAKRPVIYSGGGVISSGLQAVQLLRD
LVQLGDFPITSTLMGLGAYPASGKNWLGMLGMHGTYEANMAMHDCDVMLAVGARFDDRITGRLDAFAPHARIIHIDVDPS
SINKIVKVEVPIIGDVADVLGDMMQCLHRQQPIFKSEGRDAWWKQIDCWRARNSLAYPKKKDIIMPQYALERLYALTKDV
NAYITTDVGQHQMWAAQYYHFDEPNHWMTSGGLGTMGYGLPAAIGVQIAHPDAFVVCISGDASIQMNIQELATAMQYNTP
VKIFILNNQYMGMVRQWQQLLHGNRLSHSYTESMPDFVKLAQAYGGIGIHCSKPDELDDKIQQMIDCDKPVLFDCRVDNL
ENCFPMIPSGCAHNDMLLPDEAHDEAVANAISAEGQCLV
>Mature_598_residues
TNHPRMQESANGKKMLGAEMVVQALIDQGVKHLFGYPGGAVLPIFDVLYQQDSLQHILVRHEQAAGHAAEGYARSTGKVG
VMLVTSGPGATNAVTPLQDALMDSIPLVCFSGQVSTTVIGTNGFQEADTVGITKSCTKQNWLVKDVNDLAAIIHEAFAVA
QSGRPGPVLVDIPKDIQLASGVYRAPQVLVSRHFSPQLERNIEAIECAVSLLEEAKRPVIYSGGGVISSGLQAVQLLRDL
VQLGDFPITSTLMGLGAYPASGKNWLGMLGMHGTYEANMAMHDCDVMLAVGARFDDRITGRLDAFAPHARIIHIDVDPSS
INKIVKVEVPIIGDVADVLGDMMQCLHRQQPIFKSEGRDAWWKQIDCWRARNSLAYPKKKDIIMPQYALERLYALTKDVN
AYITTDVGQHQMWAAQYYHFDEPNHWMTSGGLGTMGYGLPAAIGVQIAHPDAFVVCISGDASIQMNIQELATAMQYNTPV
KIFILNNQYMGMVRQWQQLLHGNRLSHSYTESMPDFVKLAQAYGGIGIHCSKPDELDDKIQQMIDCDKPVLFDCRVDNLE
NCFPMIPSGCAHNDMLLPDEAHDEAVANAISAEGQCLV

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=572, Percent_Identity=26.2237762237762, Blast_Score=164, Evalue=3e-40,
Organism=Homo sapiens, GI21361361, Length=572, Percent_Identity=25.3496503496504, Blast_Score=147, Evalue=3e-35,
Organism=Escherichia coli, GI87081685, Length=569, Percent_Identity=51.6695957820738, Blast_Score=592, Evalue=1e-170,
Organism=Escherichia coli, GI1790104, Length=571, Percent_Identity=41.8563922942207, Blast_Score=456, Evalue=1e-129,
Organism=Escherichia coli, GI1786717, Length=566, Percent_Identity=32.6855123674912, Blast_Score=293, Evalue=3e-80,
Organism=Escherichia coli, GI1787096, Length=555, Percent_Identity=25.7657657657658, Blast_Score=165, Evalue=8e-42,
Organism=Escherichia coli, GI1788716, Length=559, Percent_Identity=25.9391771019678, Blast_Score=149, Evalue=6e-37,
Organism=Caenorhabditis elegans, GI17531299, Length=583, Percent_Identity=25.9005145797599, Blast_Score=152, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI17531301, Length=585, Percent_Identity=25.982905982906, Blast_Score=152, Evalue=6e-37,
Organism=Caenorhabditis elegans, GI17542570, Length=546, Percent_Identity=26.1904761904762, Blast_Score=123, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6323755, Length=601, Percent_Identity=44.2595673876872, Blast_Score=500, Evalue=1e-142,
Organism=Saccharomyces cerevisiae, GI6320816, Length=482, Percent_Identity=23.8589211618257, Blast_Score=96, Evalue=1e-20,
Organism=Drosophila melanogaster, GI19922626, Length=570, Percent_Identity=25.2631578947368, Blast_Score=148, Evalue=1e-35,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 65527; Mature: 65396

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNHPRMQESANGKKMLGAEMVVQALIDQGVKHLFGYPGGAVLPIFDVLYQQDSLQHILV
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHH
RHEQAAGHAAEGYARSTGKVGVMLVTSGPGATNAVTPLQDALMDSIPLVCFSGQVSTTVI
HHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHCCCEEEECCCEEEEEE
GTNGFQEADTVGITKSCTKQNWLVKDVNDLAAIIHEAFAVAQSGRPGPVLVDIPKDIQLA
ECCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHH
SGVYRAPQVLVSRHFSPQLERNIEAIECAVSLLEEAKRPVIYSGGGVISSGLQAVQLLRD
CCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHH
LVQLGDFPITSTLMGLGAYPASGKNWLGMLGMHGTYEANMAMHDCDVMLAVGARFDDRIT
HHHHCCCCHHHHHHHCCCCCCCCCCCEEECCCCCCEECCCEEECCCEEEEECCCCCCCCC
GRLDAFAPHARIIHIDVDPSSINKIVKVEVPIIGDVADVLGDMMQCLHRQQPIFKSEGRD
CCHHCCCCCEEEEEEECCHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHCCCCC
AWWKQIDCWRARNSLAYPKKKDIIMPQYALERLYALTKDVNAYITTDVGQHQMWAAQYYH
HHHHHHHHHHHCCCCCCCCCCCEECHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHEE
FDEPNHWMTSGGLGTMGYGLPAAIGVQIAHPDAFVVCISGDASIQMNIQELATAMQYNTP
CCCCCCEEECCCCCCCCCCCCHHHEEEEECCCEEEEEECCCCEEEEEHHHHHHHHHCCCC
VKIFILNNQYMGMVRQWQQLLHGNRLSHSYTESMPDFVKLAQAYGGIGIHCSKPDELDDK
EEEEEECCHHHHHHHHHHHHHCCCCCCCHHHHCCHHHHHHHHHHCCCEEECCCCCHHHHH
IQQMIDCDKPVLFDCRVDNLENCFPMIPSGCAHNDMLLPDEAHDEAVANAISAEGQCLV
HHHHHCCCCCEEEEECCCCHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TNHPRMQESANGKKMLGAEMVVQALIDQGVKHLFGYPGGAVLPIFDVLYQQDSLQHILV
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHH
RHEQAAGHAAEGYARSTGKVGVMLVTSGPGATNAVTPLQDALMDSIPLVCFSGQVSTTVI
HHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHCCCEEEECCCEEEEEE
GTNGFQEADTVGITKSCTKQNWLVKDVNDLAAIIHEAFAVAQSGRPGPVLVDIPKDIQLA
ECCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHH
SGVYRAPQVLVSRHFSPQLERNIEAIECAVSLLEEAKRPVIYSGGGVISSGLQAVQLLRD
CCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHH
LVQLGDFPITSTLMGLGAYPASGKNWLGMLGMHGTYEANMAMHDCDVMLAVGARFDDRIT
HHHHCCCCHHHHHHHCCCCCCCCCCCEEECCCCCCEECCCEEECCCEEEEECCCCCCCCC
GRLDAFAPHARIIHIDVDPSSINKIVKVEVPIIGDVADVLGDMMQCLHRQQPIFKSEGRD
CCHHCCCCCEEEEEEECCHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHCCCCC
AWWKQIDCWRARNSLAYPKKKDIIMPQYALERLYALTKDVNAYITTDVGQHQMWAAQYYH
HHHHHHHHHHHCCCCCCCCCCCEECHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHEE
FDEPNHWMTSGGLGTMGYGLPAAIGVQIAHPDAFVVCISGDASIQMNIQELATAMQYNTP
CCCCCCEEECCCCCCCCCCCCHHHEEEEECCCEEEEEECCCCEEEEEHHHHHHHHHCCCC
VKIFILNNQYMGMVRQWQQLLHGNRLSHSYTESMPDFVKLAQAYGGIGIHCSKPDELDDK
EEEEEECCHHHHHHHHHHHHHCCCCCCCHHHHCCHHHHHHHHHHCCCEEECCCCCHHHHH
IQQMIDCDKPVLFDCRVDNLENCFPMIPSGCAHNDMLLPDEAHDEAVANAISAEGQCLV
HHHHHCCCCCEEEEECCCCHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6308579; 1630901; 9278503; 3891724; 9298646 [H]