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The map label for this gene is nuoE [H]

Identifier: 49475654

GI number: 49475654

Start: 1007639

End: 1008307

Strand: Reverse

Name: nuoE [H]

Synonym: BH08910

Alternate gene names: 49475654

Gene position: 1008307-1007639 (Counterclockwise)

Preceding gene: 49475655

Following gene: 49475653

Centisome position: 52.22

GC content: 39.46

Gene sequence:

>669_bases
ATGTCTGTGCGTCGTCTTGCAGATGATGTCTACCAGCCTGCAGAGTTTTCTTTTACAAAAGAAAATCAGATATGGGTAAA
AAATACTATAGAAAAATATCCTGTAGGGCGTGAGCAGTCTGCTGTTATTCCATTATTAATGCGTGCTCAAGAGCAAGATG
GCTGGGTGACACGTGCTGCGATTGAGCATATCGCTCAGATTCTTTCCATGGCTTATATTCGTGTTCTAGAAATTGCAACT
TTTTATACTCAGTTTCAGCTTCAGCCAGTTGGAACAAAGGCGCATATTCAAGTTTGTGGTACGACTCCTTGTATGTTACG
TGGATCTGAGGAACTGATTAAAGTTTGTCAGAAAAAAATTCATCATAAACCTTTTGTGACCAATCAAGATGGAAGCTTAT
CATGGGAAGAAGTGGAGTGTCTTGGCGCTTGTGTCAATGCTCCCATGGTCATGATTTTTAAAGATACTTATGAAGATCTT
ACAGCTAAGCGTCTTGAAGAAATTATTGATGCATTTGAAGCGGGGAAAGGTGCTGAGATAGCTGTCGGTCCGCAAAATAG
TCGCAAATCATCAGAACCGATTAGTGGTTTAACTTCTCTCATTGATGAAAAAGAACAGAAGTCGCTTAAGTCTTTAAAGA
AAAAGGATAAAAAAGAAATGGGAGCATAG

Upstream 100 bases:

>100_bases
ATCTTCAAGCCATGGATTTTTTGACTCGAGGTCATATGTTGGCTGATGCTACAGCTATTTTGGGTTCGATTGATATTGTG
TTTGGGGAGGTTGATCGCTA

Downstream 100 bases:

>100_bases
TGAATATTTTTTCTTGAAAAGATATTTTATCATTTTATCCATTCTCTTAAGGTTTAACTAAAAGTCAGAATTTTTGAAAA
GATGTTTTAAAATTGTATTT

Product: NADH dehydrogenase subunit E

Products: NA

Alternate protein names: NADH dehydrogenase I subunit E 1; NDH-1 subunit E 1 [H]

Number of amino acids: Translated: 222; Mature: 221

Protein sequence:

>222_residues
MSVRRLADDVYQPAEFSFTKENQIWVKNTIEKYPVGREQSAVIPLLMRAQEQDGWVTRAAIEHIAQILSMAYIRVLEIAT
FYTQFQLQPVGTKAHIQVCGTTPCMLRGSEELIKVCQKKIHHKPFVTNQDGSLSWEEVECLGACVNAPMVMIFKDTYEDL
TAKRLEEIIDAFEAGKGAEIAVGPQNSRKSSEPISGLTSLIDEKEQKSLKSLKKKDKKEMGA

Sequences:

>Translated_222_residues
MSVRRLADDVYQPAEFSFTKENQIWVKNTIEKYPVGREQSAVIPLLMRAQEQDGWVTRAAIEHIAQILSMAYIRVLEIAT
FYTQFQLQPVGTKAHIQVCGTTPCMLRGSEELIKVCQKKIHHKPFVTNQDGSLSWEEVECLGACVNAPMVMIFKDTYEDL
TAKRLEEIIDAFEAGKGAEIAVGPQNSRKSSEPISGLTSLIDEKEQKSLKSLKKKDKKEMGA
>Mature_221_residues
SVRRLADDVYQPAEFSFTKENQIWVKNTIEKYPVGREQSAVIPLLMRAQEQDGWVTRAAIEHIAQILSMAYIRVLEIATF
YTQFQLQPVGTKAHIQVCGTTPCMLRGSEELIKVCQKKIHHKPFVTNQDGSLSWEEVECLGACVNAPMVMIFKDTYEDLT
AKRLEEIIDAFEAGKGAEIAVGPQNSRKSSEPISGLTSLIDEKEQKSLKSLKKKDKKEMGA

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1905

COG function: function code C; NADH:ubiquinone oxidoreductase 24 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 24 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI222080062, Length=187, Percent_Identity=49.1978609625668, Blast_Score=178, Evalue=4e-45,
Organism=Escherichia coli, GI1788621, Length=135, Percent_Identity=34.0740740740741, Blast_Score=80, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17561328, Length=186, Percent_Identity=47.8494623655914, Blast_Score=177, Evalue=3e-45,
Organism=Drosophila melanogaster, GI18859877, Length=188, Percent_Identity=46.8085106382979, Blast_Score=170, Evalue=8e-43,
Organism=Drosophila melanogaster, GI21355353, Length=194, Percent_Identity=37.1134020618557, Blast_Score=137, Evalue=5e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002023
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01257 Complex1_24kDa [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 24978; Mature: 24847

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: PS01099 COMPLEX1_24K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVRRLADDVYQPAEFSFTKENQIWVKNTIEKYPVGREQSAVIPLLMRAQEQDGWVTRAA
CCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHH
IEHIAQILSMAYIRVLEIATFYTQFQLQPVGTKAHIQVCGTTPCMLRGSEELIKVCQKKI
HHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCEEEEEECCCCCHHCCCCHHHHHHHHHHH
HHKPFVTNQDGSLSWEEVECLGACVNAPMVMIFKDTYEDLTAKRLEEIIDAFEAGKGAEI
CCCCCEECCCCCCCHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCEE
AVGPQNSRKSSEPISGLTSLIDEKEQKSLKSLKKKDKKEMGA
EECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SVRRLADDVYQPAEFSFTKENQIWVKNTIEKYPVGREQSAVIPLLMRAQEQDGWVTRAA
CHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHH
IEHIAQILSMAYIRVLEIATFYTQFQLQPVGTKAHIQVCGTTPCMLRGSEELIKVCQKKI
HHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCEEEEEECCCCCHHCCCCHHHHHHHHHHH
HHKPFVTNQDGSLSWEEVECLGACVNAPMVMIFKDTYEDLTAKRLEEIIDAFEAGKGAEI
CCCCCEECCCCCCCHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCEE
AVGPQNSRKSSEPISGLTSLIDEKEQKSLKSLKKKDKKEMGA
EECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]