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The map label for this gene is birA [H]

Identifier: 49475644

GI number: 49475644

Start: 995720

End: 996529

Strand: Reverse

Name: birA [H]

Synonym: BH08810

Alternate gene names: 49475644

Gene position: 996529-995720 (Counterclockwise)

Preceding gene: 49475645

Following gene: 49475643

Centisome position: 51.61

GC content: 34.94

Gene sequence:

>810_bases
ATGAAAATGGATTATATCTTATCAGATTTTGCACAAAAACAAGGATACGCTGTTGAATCGTATGAAATTGTTGATTCAAC
AAATTACATTGCACAGCAAAAAGCTCACGCTGGTCATCATGGTTATCTTTGGGTTGTTGCAAAAGAGCAATCACAGGGAA
GAGCAAGAAGAGGGAGAACATGGTCTAGTCCGAAAGGGAACCTTTATTCTAGTCTTTTGTTAGTTGAGGATATTGTTCAT
CAAACAGCGGCTCAGCTTGGTTTTGTTGCTGGGGTAAGTGTGGTAGAGACAATAAAACAATTTATAAAAGATGAAAAGCA
AACGAGCGATATTGTTAGCTTAAAATGGCCAAATGATATTTTGCTAAGAGGTGCTAAAAGCTCAGGAATTTTGCTTGAGA
TTTTTAAATTACCGTCACAACAGTATGCATTGGTTATTGGTATTGGAATCAATGTAAAATATCACTATGAAGATGCGCCG
TACCCTACTTCAAGTTTAAAAAATATTGGTTTGGATATTGAAACAGAGCAGTTATTTACGGTTTTGACAGAGTATTTTTC
TAAAAATTACCTTCTTTGGAAACAACTAGGGGGATGTGATAGAATACGTGAAAAGTGGCTTTTATATTCCGCTCATTCTG
GACAATATGTCAAAATAGTCAATGATGAAAAAATCATTGAAGGTATTTTTGATGGTCTTGATAATGATTTTAACTGCGTC
ATAAAACAAAAAAATGGTCAGCAAGCAGTTATAACAGCTGGAGATGTTCATTTTGGTTCGTCTGCTTCTGTAAATGCAGG
TTGTTATTAA

Upstream 100 bases:

>100_bases
GGTTTTGTCTCAGTTTTTCTGCATTCTTTATTTTATCTTATACGCTTTTTGGGATTTGGTTTGCTGACTTGGCAGAACGA
GCAGCTACTGCATTGTTTTA

Downstream 100 bases:

>100_bases
TTGATAAAACTTTCTTTCATCATCAAATTGTTTTAAGAGGATATGTGTATGGTTGCAGCCAACAAAGATGAGTTTGTTTT
TTTACCTTTAGGAGGAGTAG

Product: biotin-protein ligase birA

Products: NA

Alternate protein names: Biotin--protein ligase [H]

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MKMDYILSDFAQKQGYAVESYEIVDSTNYIAQQKAHAGHHGYLWVVAKEQSQGRARRGRTWSSPKGNLYSSLLLVEDIVH
QTAAQLGFVAGVSVVETIKQFIKDEKQTSDIVSLKWPNDILLRGAKSSGILLEIFKLPSQQYALVIGIGINVKYHYEDAP
YPTSSLKNIGLDIETEQLFTVLTEYFSKNYLLWKQLGGCDRIREKWLLYSAHSGQYVKIVNDEKIIEGIFDGLDNDFNCV
IKQKNGQQAVITAGDVHFGSSASVNAGCY

Sequences:

>Translated_269_residues
MKMDYILSDFAQKQGYAVESYEIVDSTNYIAQQKAHAGHHGYLWVVAKEQSQGRARRGRTWSSPKGNLYSSLLLVEDIVH
QTAAQLGFVAGVSVVETIKQFIKDEKQTSDIVSLKWPNDILLRGAKSSGILLEIFKLPSQQYALVIGIGINVKYHYEDAP
YPTSSLKNIGLDIETEQLFTVLTEYFSKNYLLWKQLGGCDRIREKWLLYSAHSGQYVKIVNDEKIIEGIFDGLDNDFNCV
IKQKNGQQAVITAGDVHFGSSASVNAGCY
>Mature_269_residues
MKMDYILSDFAQKQGYAVESYEIVDSTNYIAQQKAHAGHHGYLWVVAKEQSQGRARRGRTWSSPKGNLYSSLLLVEDIVH
QTAAQLGFVAGVSVVETIKQFIKDEKQTSDIVSLKWPNDILLRGAKSSGILLEIFKLPSQQYALVIGIGINVKYHYEDAP
YPTSSLKNIGLDIETEQLFTVLTEYFSKNYLLWKQLGGCDRIREKWLLYSAHSGQYVKIVNDEKIIEGIFDGLDNDFNCV
IKQKNGQQAVITAGDVHFGSSASVNAGCY

Specific function: Activates biotin to form biotinyl-5'-adenylate and transfers the biotin moiety to biotin-accepting proteins [H]

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=238, Percent_Identity=27.7310924369748, Blast_Score=79, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004408
- InterPro:   IPR003142
- InterPro:   IPR004143 [H]

Pfam domain/function: PF02237 BPL_C; PF03099 BPL_LipA_LipB [H]

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 30141; Mature: 30141

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMDYILSDFAQKQGYAVESYEIVDSTNYIAQQKAHAGHHGYLWVVAKEQSQGRARRGRT
CCCHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHCCCCCCCEEEEEECCCCCCHHHCCCC
WSSPKGNLYSSLLLVEDIVHQTAAQLGFVAGVSVVETIKQFIKDEKQTSDIVSLKWPNDI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHH
LLRGAKSSGILLEIFKLPSQQYALVIGIGINVKYHYEDAPYPTSSLKNIGLDIETEQLFT
EEECCCCCCEEEEEECCCCCCEEEEEEEEEEEEEEECCCCCCHHHHHHCCCCCCHHHHHH
VLTEYFSKNYLLWKQLGGCDRIREKWLLYSAHSGQYVKIVNDEKIIEGIFDGLDNDFNCV
HHHHHHCCCEEEEHHCCCHHHHHHHEEEEEECCCCEEEEECCHHHHHHHHHCCCCCEEEE
IKQKNGQQAVITAGDVHFGSSASVNAGCY
EEECCCCEEEEEECCEECCCCCCCCCCCC
>Mature Secondary Structure
MKMDYILSDFAQKQGYAVESYEIVDSTNYIAQQKAHAGHHGYLWVVAKEQSQGRARRGRT
CCCHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHCCCCCCCEEEEEECCCCCCHHHCCCC
WSSPKGNLYSSLLLVEDIVHQTAAQLGFVAGVSVVETIKQFIKDEKQTSDIVSLKWPNDI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHH
LLRGAKSSGILLEIFKLPSQQYALVIGIGINVKYHYEDAPYPTSSLKNIGLDIETEQLFT
EEECCCCCCEEEEEECCCCCCEEEEEEEEEEEEEEECCCCCCHHHHHHCCCCCCHHHHHH
VLTEYFSKNYLLWKQLGGCDRIREKWLLYSAHSGQYVKIVNDEKIIEGIFDGLDNDFNCV
HHHHHHCCCEEEEHHCCCHHHHHHHEEEEEECCCCEEEEECCHHHHHHHHHCCCCCEEEE
IKQKNGQQAVITAGDVHFGSSASVNAGCY
EEECCCCEEEEEECCEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8422400 [H]