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The map label for this gene is nifS2 [H]

Identifier: 49475628

GI number: 49475628

Start: 969451

End: 970605

Strand: Reverse

Name: nifS2 [H]

Synonym: BH08650

Alternate gene names: 49475628

Gene position: 970605-969451 (Counterclockwise)

Preceding gene: 49475632

Following gene: 49475627

Centisome position: 50.26

GC content: 37.66

Gene sequence:

>1155_bases
ATGGTTGTAAAACGTCGATATTTTGATCATAACGCGACAACACCGCTTAAGAAGGCGGCAGAGGTAGCGTTACTGGAATC
TTTAGAGATCTTTGGTAATCCCTCATCCGTTCATACGGAAGGACGTGCTGCTAAAGCTTTATTACAAAAGGCACGTCGAC
AAATTGCTAAAAGTCTTAACGCAAATCCAGATCATGTTGTATTCACGTCTGGTGCAAGTGAAGCAGCCATGACTTTATTA
ACACCCTTTTATAATATGGGAAGCTCTAAAGTTCAATTTTCCCATCTTTACATTGGGGCAACTGAACATCCATCTGTTGC
AGAAGGTGGACGTTTTCCTAAAGAATTCATTAGTACAATTGCTGTTGATCAAGATGGACTTATTCAACAAGATAAATTGC
TCTCTCTCTTAAGCTTTCATGATAAAACAAAGGGACTTCCTCTTGTTGCTATTCAAGCCGCAAATGGTGAAACGGGTGTT
ATGCAACAAATAAAAGAAATAGCCGCTATTGTTAGGAACTGTGGAGGTACTCTTATTGTTGATTTGACACAGTATATAGA
TAAAAATTTTGTTGATGTTCATCAGATGGAAGGAGATTTTTTTATATTGTCTGCGCATAAAATTGGTGGTCCTAAAGGGA
TTGGTGCTTTTGTTTCATGCGGAAACCTTTTGATGCCGTATCCTCTTATTGTTGGAGGAGGGCAAGAAAAAGGTTTGCGT
GGAGGAACAGAAGCATTGCCACTTATTGCTGCTTTTGGGGCAGCAATGGCTGATTGCTATAACCAAGAAGAAATAAAGCA
ATTACTCTATTTACGCCATAAATTAGAAGATGGAATACAGAAAATATGTCATGATGTTGAAATCTTTGGCAAAAAAGTTC
AGCGTTTACCAAACACAACTTATTTCACTGTGCGCAACATAAAAGCTGAAACTTTACAAATTAGTTTTGATTTGGCAGGA
TTTTCGGTATCAGCAGGCTCTGCTTGCTCTTCAGGAAAAGTAAAACAAAGCCGTGTTTTGGAGGCAATGGGATATGATGT
TCCAAATGGTGCAATTCGTATTTCAACAGGACGGTGCACGACTTCCAAAGATATTGATGATTTTTTATTGTTTTTTTCTC
AAATTATAAATAAGAAAAAAGCTGGAACTTTATAA

Upstream 100 bases:

>100_bases
TTTCATGAACCGTTAGATATTCTGTTTTGGGTGTGTTTTTCAAGAAAATTGCGCCACTTTTTAAAAATCAATTCTTAAAG
ATTAATGATTACGATATAAA

Downstream 100 bases:

>100_bases
AAACTAGAATTATTTTAAAAAATAAAATAAGCTTGATTGAAAATTTCCCACTTTGCATATAACCTAGTAAATGAATTGCT
TTCTTCATTCTTTTAAAAGA

Product: nitrogenase cofactor synthesis protein nifS

Products: NA

Alternate protein names: NifS protein homolog [H]

Number of amino acids: Translated: 384; Mature: 384

Protein sequence:

>384_residues
MVVKRRYFDHNATTPLKKAAEVALLESLEIFGNPSSVHTEGRAAKALLQKARRQIAKSLNANPDHVVFTSGASEAAMTLL
TPFYNMGSSKVQFSHLYIGATEHPSVAEGGRFPKEFISTIAVDQDGLIQQDKLLSLLSFHDKTKGLPLVAIQAANGETGV
MQQIKEIAAIVRNCGGTLIVDLTQYIDKNFVDVHQMEGDFFILSAHKIGGPKGIGAFVSCGNLLMPYPLIVGGGQEKGLR
GGTEALPLIAAFGAAMADCYNQEEIKQLLYLRHKLEDGIQKICHDVEIFGKKVQRLPNTTYFTVRNIKAETLQISFDLAG
FSVSAGSACSSGKVKQSRVLEAMGYDVPNGAIRISTGRCTTSKDIDDFLLFFSQIINKKKAGTL

Sequences:

>Translated_384_residues
MVVKRRYFDHNATTPLKKAAEVALLESLEIFGNPSSVHTEGRAAKALLQKARRQIAKSLNANPDHVVFTSGASEAAMTLL
TPFYNMGSSKVQFSHLYIGATEHPSVAEGGRFPKEFISTIAVDQDGLIQQDKLLSLLSFHDKTKGLPLVAIQAANGETGV
MQQIKEIAAIVRNCGGTLIVDLTQYIDKNFVDVHQMEGDFFILSAHKIGGPKGIGAFVSCGNLLMPYPLIVGGGQEKGLR
GGTEALPLIAAFGAAMADCYNQEEIKQLLYLRHKLEDGIQKICHDVEIFGKKVQRLPNTTYFTVRNIKAETLQISFDLAG
FSVSAGSACSSGKVKQSRVLEAMGYDVPNGAIRISTGRCTTSKDIDDFLLFFSQIINKKKAGTL
>Mature_384_residues
MVVKRRYFDHNATTPLKKAAEVALLESLEIFGNPSSVHTEGRAAKALLQKARRQIAKSLNANPDHVVFTSGASEAAMTLL
TPFYNMGSSKVQFSHLYIGATEHPSVAEGGRFPKEFISTIAVDQDGLIQQDKLLSLLSFHDKTKGLPLVAIQAANGETGV
MQQIKEIAAIVRNCGGTLIVDLTQYIDKNFVDVHQMEGDFFILSAHKIGGPKGIGAFVSCGNLLMPYPLIVGGGQEKGLR
GGTEALPLIAAFGAAMADCYNQEEIKQLLYLRHKLEDGIQKICHDVEIFGKKVQRLPNTTYFTVRNIKAETLQISFDLAG
FSVSAGSACSSGKVKQSRVLEAMGYDVPNGAIRISTGRCTTSKDIDDFLLFFSQIINKKKAGTL

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=378, Percent_Identity=31.4814814814815, Blast_Score=155, Evalue=5e-38,
Organism=Homo sapiens, GI156713448, Length=411, Percent_Identity=29.4403892944039, Blast_Score=147, Evalue=2e-35,
Organism=Escherichia coli, GI48994898, Length=372, Percent_Identity=33.0645161290323, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1787970, Length=205, Percent_Identity=27.3170731707317, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI25143064, Length=372, Percent_Identity=31.1827956989247, Blast_Score=146, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17533177, Length=326, Percent_Identity=27.6073619631902, Blast_Score=97, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6319831, Length=373, Percent_Identity=34.0482573726542, Blast_Score=167, Evalue=2e-42,
Organism=Drosophila melanogaster, GI20129463, Length=376, Percent_Identity=31.9148936170213, Blast_Score=146, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR010240
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 41656; Mature: 41656

Theoretical pI: Translated: 8.75; Mature: 8.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVVKRRYFDHNATTPLKKAAEVALLESLEIFGNPSSVHTEGRAAKALLQKARRQIAKSLN
CCCCCEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
ANPDHVVFTSGASEAAMTLLTPFYNMGSSKVQFSHLYIGATEHPSVAEGGRFPKEFISTI
CCCCEEEEECCCCHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCHHHHHHHH
AVDQDGLIQQDKLLSLLSFHDKTKGLPLVAIQAANGETGVMQQIKEIAAIVRNCGGTLIV
HCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEE
DLTQYIDKNFVDVHQMEGDFFILSAHKIGGPKGIGAFVSCGNLLMPYPLIVGGGQEKGLR
EHHHHHCCCCCCCEECCCCEEEEEECCCCCCCCCHHHHHCCCHHCCCCEEEECCCCCCCC
GGTEALPLIAAFGAAMADCYNQEEIKQLLYLRHKLEDGIQKICHDVEIFGKKVQRLPNTT
CCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
YFTVRNIKAETLQISFDLAGFSVSAGSACSSGKVKQSRVLEAMGYDVPNGAIRISTGRCT
EEEEECCEEEEEEEEEEECCEEECCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCC
TSKDIDDFLLFFSQIINKKKAGTL
CCCCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MVVKRRYFDHNATTPLKKAAEVALLESLEIFGNPSSVHTEGRAAKALLQKARRQIAKSLN
CCCCCEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
ANPDHVVFTSGASEAAMTLLTPFYNMGSSKVQFSHLYIGATEHPSVAEGGRFPKEFISTI
CCCCEEEEECCCCHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCHHHHHHHH
AVDQDGLIQQDKLLSLLSFHDKTKGLPLVAIQAANGETGVMQQIKEIAAIVRNCGGTLIV
HCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEE
DLTQYIDKNFVDVHQMEGDFFILSAHKIGGPKGIGAFVSCGNLLMPYPLIVGGGQEKGLR
EHHHHHCCCCCCCEECCCCEEEEEECCCCCCCCCHHHHHCCCHHCCCCEEEECCCCCCCC
GGTEALPLIAAFGAAMADCYNQEEIKQLLYLRHKLEDGIQKICHDVEIFGKKVQRLPNTT
CCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
YFTVRNIKAETLQISFDLAGFSVSAGSACSSGKVKQSRVLEAMGYDVPNGAIRISTGRCT
EEEEECCEEEEEEEEEEECCEEECCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCC
TSKDIDDFLLFFSQIINKKKAGTL
CCCCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10930739 [H]