Click here to switch to the map view.

The map label for this gene is prfB [H]

Identifier: 49475632

GI number: 49475632

Start: 973883

End: 974851

Strand: Reverse

Name: prfB [H]

Synonym: BH08690

Alternate gene names: 49475632

Gene position: 974851-973883 (Counterclockwise)

Preceding gene: 49475633

Following gene: 49475628

Centisome position: 50.48

GC content: 40.25

Gene sequence:

>969_bases
ATGATGCGTGAGCGTCAGCGTCTTGATGATTCCATTAATGGCATTCGATCATTTACAAAAACACTTGAAGAGTGCATTGA
GTTAATCGCAATGGGTGAAGAAGAAAGTGATATCGAAATTATCACGGATGCAGAAAATTCAATACGCAATCTTAAGAGTG
AAATAGATAAAAGACAAATTGATGCGCTTCTTTCAGGAGAAGCCGACCAGAATGATACTTATCTAGAAGTGCATGCTGGT
GCAGGAGGTACGGAAAGCCAAGATTGGGCTTCTATGCTGTTACGCATGTATATGCGCTGGGCTGAACAGCATAAAATGAA
GGTTGAAATCTTAGAAATTCACGATGGAGAAGAAGCCGGAATAAAATCAGCGACTCTTCTTGTAAAAGGGCATAATGCGT
ATGGTATGTTGAAGACGGAATCAGGTGTGCATCGTTTGGTACGCATTTCGCCGTTTGATTCAAATGCAAAACGTCATACT
TCTTTTGCAAGCATTTGGGTCTACCCAGTTATTGACGATAATATTGAAGTTGATGTTTCAGAAGCAGATGTGCGTATTGA
TACATATCGTGCGTCAGGAGCTGGAGGACAACATGTCAATACGACAGATTCTGCAGTGCGCATCACACATATAAAAACAG
GTATTGTTGTTCAATGTCAAACAGAGCGTTCTCAGCATAAAAATCGAGCAATTGCCTGGTCTATGTTGCGTGCAAGACTT
TATGAAGAAGAGCTCAAAAAGAGAGAAGAAGAAACAAATGCTGTTGAAGCTTCTAAAACAGAAATCGGATGGGGACATCA
AATTAGATCATATGTTCTTCAGCCCTATCAACTTGTCAAAGATTTACGCACAGGTGTTGAAAATACGGATCCCCAATCCG
TACTTAATGGCAATTTAGATTCCTTTATAGAAGCTGCACTTGCTCGACGCATCTACGGAAAAGACCCAGAAATTGAGGAT
ATTGATTAA

Upstream 100 bases:

>100_bases
GGGCATCTTTGACTGGGATCAATCACTCAAACGTCTCGAATATCTCAATCAAAAAGCAGAAGATCCAACACTTTGGGATA
ATGCACAACAAGCGCAAGAT

Downstream 100 bases:

>100_bases
TCAGATATAATCTTTTCAACTTTTATAGTGTGTTTGCTTAATCGGTTGTTCATTCCGTTCAACCAGTTTTTACCAATTAA
AAACCAATTAGAGGAAGAGT

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MMRERQRLDDSINGIRSFTKTLEECIELIAMGEEESDIEIITDAENSIRNLKSEIDKRQIDALLSGEADQNDTYLEVHAG
AGGTESQDWASMLLRMYMRWAEQHKMKVEILEIHDGEEAGIKSATLLVKGHNAYGMLKTESGVHRLVRISPFDSNAKRHT
SFASIWVYPVIDDNIEVDVSEADVRIDTYRASGAGGQHVNTTDSAVRITHIKTGIVVQCQTERSQHKNRAIAWSMLRARL
YEEELKKREEETNAVEASKTEIGWGHQIRSYVLQPYQLVKDLRTGVENTDPQSVLNGNLDSFIEAALARRIYGKDPEIED
ID

Sequences:

>Translated_322_residues
MMRERQRLDDSINGIRSFTKTLEECIELIAMGEEESDIEIITDAENSIRNLKSEIDKRQIDALLSGEADQNDTYLEVHAG
AGGTESQDWASMLLRMYMRWAEQHKMKVEILEIHDGEEAGIKSATLLVKGHNAYGMLKTESGVHRLVRISPFDSNAKRHT
SFASIWVYPVIDDNIEVDVSEADVRIDTYRASGAGGQHVNTTDSAVRITHIKTGIVVQCQTERSQHKNRAIAWSMLRARL
YEEELKKREEETNAVEASKTEIGWGHQIRSYVLQPYQLVKDLRTGVENTDPQSVLNGNLDSFIEAALARRIYGKDPEIED
ID
>Mature_322_residues
MMRERQRLDDSINGIRSFTKTLEECIELIAMGEEESDIEIITDAENSIRNLKSEIDKRQIDALLSGEADQNDTYLEVHAG
AGGTESQDWASMLLRMYMRWAEQHKMKVEILEIHDGEEAGIKSATLLVKGHNAYGMLKTESGVHRLVRISPFDSNAKRHT
SFASIWVYPVIDDNIEVDVSEADVRIDTYRASGAGGQHVNTTDSAVRITHIKTGIVVQCQTERSQHKNRAIAWSMLRARL
YEEELKKREEETNAVEASKTEIGWGHQIRSYVLQPYQLVKDLRTGVENTDPQSVLNGNLDSFIEAALARRIYGKDPEIED
ID

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=272, Percent_Identity=40.4411764705882, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI34577120, Length=249, Percent_Identity=39.3574297188755, Blast_Score=149, Evalue=3e-36,
Organism=Homo sapiens, GI166795305, Length=174, Percent_Identity=43.1034482758621, Blast_Score=125, Evalue=6e-29,
Organism=Escherichia coli, GI2367172, Length=307, Percent_Identity=52.1172638436482, Blast_Score=329, Evalue=1e-91,
Organism=Escherichia coli, GI1787462, Length=241, Percent_Identity=44.8132780082988, Blast_Score=192, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI17542784, Length=237, Percent_Identity=34.5991561181435, Blast_Score=115, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6321295, Length=260, Percent_Identity=40, Blast_Score=149, Evalue=4e-37,
Organism=Drosophila melanogaster, GI19921226, Length=244, Percent_Identity=38.1147540983607, Blast_Score=148, Evalue=4e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 36475; Mature: 36475

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRERQRLDDSINGIRSFTKTLEECIELIAMGEEESDIEIITDAENSIRNLKSEIDKRQI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHHHH
DALLSGEADQNDTYLEVHAGAGGTESQDWASMLLRMYMRWAEQHKMKVEILEIHDGEEAG
HHHHCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCC
IKSATLLVKGHNAYGMLKTESGVHRLVRISPFDSNAKRHTSFASIWVYPVIDDNIEVDVS
CCEEEEEEECCCCEEEEECCCCCEEEEEECCCCCCCHHCCCEEEEEEEEEECCCEEEEEC
EADVRIDTYRASGAGGQHVNTTDSAVRITHIKTGIVVQCQTERSQHKNRAIAWSMLRARL
CCCEEEEEEEECCCCCCCCCCCCCEEEEEEEECCEEEEEECHHHHHHHHHHHHHHHHHHH
YEEELKKREEETNAVEASKTEIGWGHQIRSYVLQPYQLVKDLRTGVENTDPQSVLNGNLD
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCHHHHHHHHHHCCCCCCCHHHHCCCHH
SFIEAALARRIYGKDPEIEDID
HHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MMRERQRLDDSINGIRSFTKTLEECIELIAMGEEESDIEIITDAENSIRNLKSEIDKRQI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHHHH
DALLSGEADQNDTYLEVHAGAGGTESQDWASMLLRMYMRWAEQHKMKVEILEIHDGEEAG
HHHHCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCC
IKSATLLVKGHNAYGMLKTESGVHRLVRISPFDSNAKRHTSFASIWVYPVIDDNIEVDVS
CCEEEEEEECCCCEEEEECCCCCEEEEEECCCCCCCHHCCCEEEEEEEEEECCCEEEEEC
EADVRIDTYRASGAGGQHVNTTDSAVRITHIKTGIVVQCQTERSQHKNRAIAWSMLRARL
CCCEEEEEEEECCCCCCCCCCCCCEEEEEEEECCEEEEEECHHHHHHHHHHHHHHHHHHH
YEEELKKREEETNAVEASKTEIGWGHQIRSYVLQPYQLVKDLRTGVENTDPQSVLNGNLD
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCHHHHHHHHHHCCCCCCCHHHHCCCHH
SFIEAALARRIYGKDPEIEDID
HHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA