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The map label for this gene is livJ [C]

Identifier: 49475594

GI number: 49475594

Start: 935254

End: 936387

Strand: Reverse

Name: livJ [C]

Synonym: BH08290

Alternate gene names: 49475594

Gene position: 936387-935254 (Counterclockwise)

Preceding gene: 49475599

Following gene: 49475593

Centisome position: 48.49

GC content: 37.39

Gene sequence:

>1134_bases
ATGCAATTGAGGAAAATTCTTGCTATAGTCACCGCTGTCATGACACTTACAGTAAATGTTTATGCGAATGAGCCCATTAA
AATTGGTGTTTACCTTCCGTTAAGTGGTCAAAATGCATTTGGAGGCCAGCTTGAAATTAGAGGTATAGAATTGGCACATA
AAAAAATCCCAGAAATATTGGGACGTAAGGTAGAACTTATTGTTGTTGATAATAAATCTGATAAAGTAGAAGCTGCCAAT
GCTGTTATGCGCTTAACCGCAAGTGATAAAGTAAATGGAATCATCGGGAGTTATGGCTCTTCACTCTCATTGGCTGGTGG
AGAAATATCTGAAAAAGCGAAAACGCCAACTATTGCAACTTCCTCAACAAGTCCGCTTGTTACACAAGGCAAGAAATATT
ACTTTCGCGCTTGCTTCGTTGATTCTTACCAAGGTATAGGAGTTGCAACTTACGCAATTCAAACTTTACATGCAAAAAAA
GCTGCTATACTCAAAGATATATCAAATGATTACGCCATTGGTCTTGCCAGTTATTTTGCGCGCTCTTTTAAAAAACTAGG
TGGTGAGATTATCTCAAATTTAAACTATAATTCTGGAGATCAGGACTTTTCAGCTGTTCTCACACAGATTATAGCACAAA
ATCCCGATATCTTATTTATTCCGTCCTATTTTTCTGAAGGTGCCATCATCATGAAACAAGCGCGTGAATTAGGTGCAAAG
TTCCGAATTATGGGCGGAGATGCCATGGATAATCCAGAAACCATTACAATTGCAGGAAAGGCTGCAGAAGGCTTTTTACA
TACAACACTCCCTTACAGCGAGGATATGCCAAATATGTCAGAAGCTGCAAAAGAATTCACAAAAGAATGGAAAGCAGCTT
ATCCTGACAAAGAACCCAATATCAATTCTGTTTTGGGATACACAAGTTATATGATGTTTATGAAAGCTATTGAAAATGCT
GGCAGTGCCGATCGTGAAAAAATTACTATAGAACTTAGCAAATTAAAAGACTTTCAAACACCCTTTGGGGATATGTCTAT
GGATGAAAACCATAATCCTACGATTCCTATCGGTGTTATTGAAATAAAGGATGGAAAACGTATCTATTTAGATGAAGTCA
AACCTGCTTTTTAA

Upstream 100 bases:

>100_bases
AGATTTTTCAAACAATGGTATTTTCATTAATGTGTAACTTGCATGATGAAAAAGTTCACGATAAAGTGTAAAAACTACAA
CTAATTAAGGAAGTTGCCCA

Downstream 100 bases:

>100_bases
AAAAATAGAAAGTCTTACTAATCAAAATACGGGAATCGGTTGAGGATTGAGGTTCAATCAAATCGTCTTGTGGTTACTTC
TTTCATTCTATATGAATTAA

Product: branched chain amino acid ABC transporter periplasmic ligand-binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MQLRKILAIVTAVMTLTVNVYANEPIKIGVYLPLSGQNAFGGQLEIRGIELAHKKIPEILGRKVELIVVDNKSDKVEAAN
AVMRLTASDKVNGIIGSYGSSLSLAGGEISEKAKTPTIATSSTSPLVTQGKKYYFRACFVDSYQGIGVATYAIQTLHAKK
AAILKDISNDYAIGLASYFARSFKKLGGEIISNLNYNSGDQDFSAVLTQIIAQNPDILFIPSYFSEGAIIMKQARELGAK
FRIMGGDAMDNPETITIAGKAAEGFLHTTLPYSEDMPNMSEAAKEFTKEWKAAYPDKEPNINSVLGYTSYMMFMKAIENA
GSADREKITIELSKLKDFQTPFGDMSMDENHNPTIPIGVIEIKDGKRIYLDEVKPAF

Sequences:

>Translated_377_residues
MQLRKILAIVTAVMTLTVNVYANEPIKIGVYLPLSGQNAFGGQLEIRGIELAHKKIPEILGRKVELIVVDNKSDKVEAAN
AVMRLTASDKVNGIIGSYGSSLSLAGGEISEKAKTPTIATSSTSPLVTQGKKYYFRACFVDSYQGIGVATYAIQTLHAKK
AAILKDISNDYAIGLASYFARSFKKLGGEIISNLNYNSGDQDFSAVLTQIIAQNPDILFIPSYFSEGAIIMKQARELGAK
FRIMGGDAMDNPETITIAGKAAEGFLHTTLPYSEDMPNMSEAAKEFTKEWKAAYPDKEPNINSVLGYTSYMMFMKAIENA
GSADREKITIELSKLKDFQTPFGDMSMDENHNPTIPIGVIEIKDGKRIYLDEVKPAF
>Mature_377_residues
MQLRKILAIVTAVMTLTVNVYANEPIKIGVYLPLSGQNAFGGQLEIRGIELAHKKIPEILGRKVELIVVDNKSDKVEAAN
AVMRLTASDKVNGIIGSYGSSLSLAGGEISEKAKTPTIATSSTSPLVTQGKKYYFRACFVDSYQGIGVATYAIQTLHAKK
AAILKDISNDYAIGLASYFARSFKKLGGEIISNLNYNSGDQDFSAVLTQIIAQNPDILFIPSYFSEGAIIMKQARELGAK
FRIMGGDAMDNPETITIAGKAAEGFLHTTLPYSEDMPNMSEAAKEFTKEWKAAYPDKEPNINSVLGYTSYMMFMKAIENA
GSADREKITIELSKLKDFQTPFGDMSMDENHNPTIPIGVIEIKDGKRIYLDEVKPAF

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=355, Percent_Identity=27.6056338028169, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI48994941, Length=263, Percent_Identity=28.8973384030418, Blast_Score=93, Evalue=3e-20,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 41188; Mature: 41188

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLRKILAIVTAVMTLTVNVYANEPIKIGVYLPLSGQNAFGGQLEIRGIELAHKKIPEIL
CCHHHHHHHHHHHHHHEEEEEECCCEEEEEEEEECCCCCCCCEEEEEEEHHHHHHHHHHH
GRKVELIVVDNKSDKVEAANAVMRLTASDKVNGIIGSYGSSLSLAGGEISEKAKTPTIAT
CCEEEEEEEECCCCCHHHHHHHEEEECCCCCCCHHHCCCCCEEECCCCHHHHCCCCEEEC
SSTSPLVTQGKKYYFRACFVDSYQGIGVATYAIQTLHAKKAAILKDISNDYAIGLASYFA
CCCCCHHHCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
RSFKKLGGEIISNLNYNSGDQDFSAVLTQIIAQNPDILFIPSYFSEGAIIMKQARELGAK
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHCCCE
FRIMGGDAMDNPETITIAGKAAEGFLHTTLPYSEDMPNMSEAAKEFTKEWKAAYPDKEPN
EEEECCCCCCCCCEEEEEECCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCC
INSVLGYTSYMMFMKAIENAGSADREKITIELSKLKDFQTPFGDMSMDENHNPTIPIGVI
HHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEHHHHHCCCCCCCCCCCCCCCCEEEEEE
EIKDGKRIYLDEVKPAF
EECCCCEEEECCCCCCC
>Mature Secondary Structure
MQLRKILAIVTAVMTLTVNVYANEPIKIGVYLPLSGQNAFGGQLEIRGIELAHKKIPEIL
CCHHHHHHHHHHHHHHEEEEEECCCEEEEEEEEECCCCCCCCEEEEEEEHHHHHHHHHHH
GRKVELIVVDNKSDKVEAANAVMRLTASDKVNGIIGSYGSSLSLAGGEISEKAKTPTIAT
CCEEEEEEEECCCCCHHHHHHHEEEECCCCCCCHHHCCCCCEEECCCCHHHHCCCCEEEC
SSTSPLVTQGKKYYFRACFVDSYQGIGVATYAIQTLHAKKAAILKDISNDYAIGLASYFA
CCCCCHHHCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
RSFKKLGGEIISNLNYNSGDQDFSAVLTQIIAQNPDILFIPSYFSEGAIIMKQARELGAK
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHCCCE
FRIMGGDAMDNPETITIAGKAAEGFLHTTLPYSEDMPNMSEAAKEFTKEWKAAYPDKEPN
EEEECCCCCCCCCEEEEEECCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCC
INSVLGYTSYMMFMKAIENAGSADREKITIELSKLKDFQTPFGDMSMDENHNPTIPIGVI
HHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEHHHHHCCCCCCCCCCCCCCCCEEEEEE
EIKDGKRIYLDEVKPAF
EECCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA