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The map label for this gene is radA [H]

Identifier: 49475321

GI number: 49475321

Start: 613276

End: 614649

Strand: Reverse

Name: radA [H]

Synonym: BH05260

Alternate gene names: 49475321

Gene position: 614649-613276 (Counterclockwise)

Preceding gene: 49475322

Following gene: 49475320

Centisome position: 31.83

GC content: 44.25

Gene sequence:

>1374_bases
ATGGCACGCATCCGTGTTCAATTTATCTGTCAAAATTGTGGGACTGTCCATTCACGCTGGGCTGGCAAATGTAATTCCTG
TGGAGAATGGAATTCCCTTATTGAAGAAAATGCGAATAGCGGCATCGGCACTGGCCCCAAGCAAAATGTTCATAAGGGGC
GTGTAGTGGCTCTTACATCTCTTTCTGGAAATATCGAAGATGCTCCACGCATCCATTCTGGCATTGCTGAACTTGATCGT
GTGACTGGAGGTGGTTTTGTACGTGGATCAGTCTTACTTGTTGGTGGCGACCCTGGTATTGGAAAGTCAACATTGCTTAC
ACAAACAGCAGCAGCCTTATCGCGAAAAGGACATCATGTTATCTACGTTTCAGGAGAAGAAGCTGTTGCACAAATCTGCC
TTCGTGCCCAAAGGCTTGATGCTGCAAATACAAAGGTTAAGCTCGCTGCTGAAACTAATGTTGAAGATATTCTTGCAACC
TTAAGCGAACACAAAAACCTTGATATGGTTATTATCGATTCTATTCAGACCCTATGGTCAGATGCAGCAGATTCAGCACC
CGGTACTGTCACACAAGTGCGCATTAGCGCTCAAGCAATGATCCGTTTTGCAAAAAAAACAGGAGCAGCTGTTGTTCTTG
TCGGCCATGTTACAAAAGAGGGACAAATTGCCGGTCCTAGGGTTGTCGAACACATGGTTGATGCTGTTCTTTATTTTGAA
GGTGAAGGTGGACATCACTATCGAATCCTTAGAACTGTAAAAAATCGCTTCGGACCAACTGATGAGATTGGTGTTTTCGA
AATGTCTGATAAAGGATTACGGGAGGTAATCAATCCATCTGAACTTTTTTTGGGTGAACGAAATGAAAAAGCACCAGGAG
CTGCTGTTTTTGCAGGAATGGAAGGAACACGTCCAATACTAGTAGAAATTCAAGCGCTTGTTGCTCCCTCTTCACTCGGA
ACACCACGACGTGCCGTTGTTGGATGGGATGGCAATCGCCTTTCAATGATTCTTGCCGTCTTAGAAGCTCATTGCGGTGT
CCGCTTTGGACAACATGATGTCTATCTGAATGTTGCAGGAGGATATCGTATATCAGAACCAGCAGCTGATTTAGCTGTTG
CCGCTGCTTTGGTATCCTCTCTCACAAATATTCCTCTCCCAACGGAGTATGTCTATTTTGGTGAAATAAGCCTTTCAGGA
GCTATCCGCGCGGTTTCTCATTCAGCACAGCGTATCAATGAAGCCCAAAAACTCGGTTTTCAAGGATCTGTTCAACCCAC
CACAACAACCAAAATGATAAAATTGCCAACTTTTCAACAAAAAACATTCTCTGAACTTTCTGAATTGATCGCCGCTCTTG
CCACAGGTAAATAA

Upstream 100 bases:

>100_bases
GACCAACAGGAACTGTTCATCTTGCTTTTCAGTCTGATTTTACACGCTTTAGCGATCTGGCAGACAGTAATTATCTTCCA
GAGCAATATGGTTAATCATT

Downstream 100 bases:

>100_bases
CGAACTACTCTACATGCATCATAATAAAGAAAAAACTCTACAAAATGAAAAAGATCCTTGCTATAAGAATAAAAAATGGC
ATAACCCCAAAATACTTGCA

Product: DNA repair protein RadA

Products: NA

Alternate protein names: DNA repair protein sms homolog [H]

Number of amino acids: Translated: 457; Mature: 456

Protein sequence:

>457_residues
MARIRVQFICQNCGTVHSRWAGKCNSCGEWNSLIEENANSGIGTGPKQNVHKGRVVALTSLSGNIEDAPRIHSGIAELDR
VTGGGFVRGSVLLVGGDPGIGKSTLLTQTAAALSRKGHHVIYVSGEEAVAQICLRAQRLDAANTKVKLAAETNVEDILAT
LSEHKNLDMVIIDSIQTLWSDAADSAPGTVTQVRISAQAMIRFAKKTGAAVVLVGHVTKEGQIAGPRVVEHMVDAVLYFE
GEGGHHYRILRTVKNRFGPTDEIGVFEMSDKGLREVINPSELFLGERNEKAPGAAVFAGMEGTRPILVEIQALVAPSSLG
TPRRAVVGWDGNRLSMILAVLEAHCGVRFGQHDVYLNVAGGYRISEPAADLAVAAALVSSLTNIPLPTEYVYFGEISLSG
AIRAVSHSAQRINEAQKLGFQGSVQPTTTTKMIKLPTFQQKTFSELSELIAALATGK

Sequences:

>Translated_457_residues
MARIRVQFICQNCGTVHSRWAGKCNSCGEWNSLIEENANSGIGTGPKQNVHKGRVVALTSLSGNIEDAPRIHSGIAELDR
VTGGGFVRGSVLLVGGDPGIGKSTLLTQTAAALSRKGHHVIYVSGEEAVAQICLRAQRLDAANTKVKLAAETNVEDILAT
LSEHKNLDMVIIDSIQTLWSDAADSAPGTVTQVRISAQAMIRFAKKTGAAVVLVGHVTKEGQIAGPRVVEHMVDAVLYFE
GEGGHHYRILRTVKNRFGPTDEIGVFEMSDKGLREVINPSELFLGERNEKAPGAAVFAGMEGTRPILVEIQALVAPSSLG
TPRRAVVGWDGNRLSMILAVLEAHCGVRFGQHDVYLNVAGGYRISEPAADLAVAAALVSSLTNIPLPTEYVYFGEISLSG
AIRAVSHSAQRINEAQKLGFQGSVQPTTTTKMIKLPTFQQKTFSELSELIAALATGK
>Mature_456_residues
ARIRVQFICQNCGTVHSRWAGKCNSCGEWNSLIEENANSGIGTGPKQNVHKGRVVALTSLSGNIEDAPRIHSGIAELDRV
TGGGFVRGSVLLVGGDPGIGKSTLLTQTAAALSRKGHHVIYVSGEEAVAQICLRAQRLDAANTKVKLAAETNVEDILATL
SEHKNLDMVIIDSIQTLWSDAADSAPGTVTQVRISAQAMIRFAKKTGAAVVLVGHVTKEGQIAGPRVVEHMVDAVLYFEG
EGGHHYRILRTVKNRFGPTDEIGVFEMSDKGLREVINPSELFLGERNEKAPGAAVFAGMEGTRPILVEIQALVAPSSLGT
PRRAVVGWDGNRLSMILAVLEAHCGVRFGQHDVYLNVAGGYRISEPAADLAVAAALVSSLTNIPLPTEYVYFGEISLSGA
IRAVSHSAQRINEAQKLGFQGSVQPTTTTKMIKLPTFQQKTFSELSELIAALATGK

Specific function: May play a role in the repair of endogenous alkylation damage [H]

COG id: COG1066

COG function: function code O; Predicted ATP-dependent serine protease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family. RadA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790850, Length=426, Percent_Identity=43.4272300469484, Blast_Score=369, Evalue=1e-103,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR014774
- InterPro:   IPR020588
- InterPro:   IPR004504
- InterPro:   IPR020568 [H]

Pfam domain/function: PF06745 KaiC [H]

EC number: NA

Molecular weight: Translated: 48717; Mature: 48586

Theoretical pI: Translated: 7.92; Mature: 7.92

Prosite motif: PS50162 RECA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARIRVQFICQNCGTVHSRWAGKCNSCGEWNSLIEENANSGIGTGPKQNVHKGRVVALTS
CCEEEEEEEECCCCCHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEE
LSGNIEDAPRIHSGIAELDRVTGGGFVRGSVLLVGGDPGIGKSTLLTQTAAALSRKGHHV
CCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCEE
IYVSGEEAVAQICLRAQRLDAANTKVKLAAETNVEDILATLSEHKNLDMVIIDSIQTLWS
EEECCHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHCCCCCEEEEHHHHHHHH
DAADSAPGTVTQVRISAQAMIRFAKKTGAAVVLVGHVTKEGQIAGPRVVEHMVDAVLYFE
HCCCCCCCCEEEEEEHHHHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHHHHEEEEEE
GEGGHHYRILRTVKNRFGPTDEIGVFEMSDKGLREVINPSELFLGERNEKAPGAAVFAGM
CCCCCCHHHHHHHHHHCCCCCCCEEEEECCHHHHHHCCCHHHHCCCCCCCCCCCEEEECC
EGTRPILVEIQALVAPSSLGTPRRAVVGWDGNRLSMILAVLEAHCGVRFGQHDVYLNVAG
CCCCEEEEEEEEHCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCEECCCEEEEEECC
GYRISEPAADLAVAAALVSSLTNIPLPTEYVYFGEISLSGAIRAVSHSAQRINEAQKLGF
CEEECCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHCCC
QGSVQPTTTTKMIKLPTFQQKTFSELSELIAALATGK
CCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ARIRVQFICQNCGTVHSRWAGKCNSCGEWNSLIEENANSGIGTGPKQNVHKGRVVALTS
CEEEEEEEECCCCCHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEE
LSGNIEDAPRIHSGIAELDRVTGGGFVRGSVLLVGGDPGIGKSTLLTQTAAALSRKGHHV
CCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCEE
IYVSGEEAVAQICLRAQRLDAANTKVKLAAETNVEDILATLSEHKNLDMVIIDSIQTLWS
EEECCHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHCCCCCEEEEHHHHHHHH
DAADSAPGTVTQVRISAQAMIRFAKKTGAAVVLVGHVTKEGQIAGPRVVEHMVDAVLYFE
HCCCCCCCCEEEEEEHHHHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHHHHEEEEEE
GEGGHHYRILRTVKNRFGPTDEIGVFEMSDKGLREVINPSELFLGERNEKAPGAAVFAGM
CCCCCCHHHHHHHHHHCCCCCCCEEEEECCHHHHHHCCCHHHHCCCCCCCCCCCEEEECC
EGTRPILVEIQALVAPSSLGTPRRAVVGWDGNRLSMILAVLEAHCGVRFGQHDVYLNVAG
CCCCEEEEEEEEHCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCEECCCEEEEEECC
GYRISEPAADLAVAAALVSSLTNIPLPTEYVYFGEISLSGAIRAVSHSAQRINEAQKLGF
CEEECCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHCCC
QGSVQPTTTTKMIKLPTFQQKTFSELSELIAALATGK
CCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA