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The map label for this gene is dnaB [H]

Identifier: 49475322

GI number: 49475322

Start: 614655

End: 616142

Strand: Reverse

Name: dnaB [H]

Synonym: BH05270

Alternate gene names: 49475322

Gene position: 616142-614655 (Counterclockwise)

Preceding gene: 49475323

Following gene: 49475321

Centisome position: 31.91

GC content: 39.25

Gene sequence:

>1488_bases
ATGGAAGAAACAAGCGTCGTCAATTTCAGCTCTTCGCAAAAAGAAGAAGCTTCTTTTCGAGAACTTCCACACAATATTGA
AGCCGAGCAAGCATTGCTTGGAGCACTTCTTATTAATAATGATGCGCTCGATCGCGTTTCAGATTTTCTAAAACCCGAAC
ATTTTTTTGAACCATTGCATCAAAAAATCTACGACGTTGTGTCTCATCTTATCAAAAATGGAAAACTTGCAAATCCGGTT
ACTATCAAGCCCTTTATTCCAGCAGAAGAAAAAATTGGAGATATTACTGTATTTAATTATGTTATTCGTTTAGCTAAAGA
AGCAGTAACAATCATTAATGCTGAGGATTATGGACGAGTCATCTATGATCTTTTTATCCGGCGATCCTTGATTAATATTG
GTAACCAAGTTGTTAATACAGCTTTTGATGCCCCTTTAGAGCTTACGCCATCCCAACAAATTGAAACTGTTGAAAATCAG
TTGTTTGAACTAGCAGAAAAGGGGAAATATGGCGGTGGGTTTGAAAATTTCAATGAGGCTATCAAAAAAGCTATTGACAT
GGCAACCGCTGCAAAAAAGCGTTCTTCACACTTATCAGGGATAGCAACCCATATTAAAACACTTGATGAAAAAATGGGAG
GATTACAACCCTCTGACCTCATTATCCTTGCTGGTCGCCCTGGTATGGGTAAAACATCACTGGCTACCAATATTGCCTTT
AATATTGCTAATGCTTGCAACCATGATGCCAACACACAAGAAAATGAGGGAGGTATTGTTGGATTCTTCTCGCTTGAAAT
GTCATCAGAACAGCTTGCAACCCGTATTATTTCGGAACAAACAGAGGTTTCCTCTTCTGACATTCGACGTGGATCTATTT
TAGACGAGCAATTTTCCAAAATCATCCGTGCCATGAATCAACTACAAAAAGCACCTCTTTACATTGACCAAACTGGTGGG
ATATCCATCGCTCAATTAGCAGCGCGCGCACGGCGTCTCAAAAGACAACACGGTTTAGATGTCTTGATTATTGACTATAT
ACAACTCATGACAAGCAATTCGAAGCGTTCGTCTGAAAACCGTGTTCAAGAAATTACAGAAATTACGACAGGACTCAAAG
CATTAGCTAAAGAACTGAATATACCCATTATTGCGCTTTCACAGCTTTCACGCCAGGTCGAAAATCGAACAGATAAACGT
CCACAACTTTCAGATTTACGGGAATCTGGTTCCATTGAGCAAGATGCCGACATTGTCCTTTTTGTGTACCGTGAAGAATA
TTACCTTAAAAATGGAGAACCAAAGATAGGCACTCTTGAATATGAAAGATGGCAAGAAACAATGGACAAAGCTTTTGGAA
AAGCGGAAGTTATTATCGCAAAACAACGTCATGGACCAACAGGAACTGTTCATCTTGCTTTTCAGTCTGATTTTACACGC
TTTAGCGATCTGGCAGACAGTAATTATCTTCCAGAGCAATATGGTTAA

Upstream 100 bases:

>100_bases
TGATTTTAACTTAACGAAAATTTATAATCCTGTTTTTATATATGCATTACTCGATTTATTGCTTAAAATAGCTAAACACA
ATCTTAGTGGAAAAAATGTT

Downstream 100 bases:

>100_bases
TCATTATGGCACGCATCCGTGTTCAATTTATCTGTCAAAATTGTGGGACTGTCCATTCACGCTGGGCTGGCAAATGTAAT
TCCTGTGGAGAATGGAATTC

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 495; Mature: 495

Protein sequence:

>495_residues
MEETSVVNFSSSQKEEASFRELPHNIEAEQALLGALLINNDALDRVSDFLKPEHFFEPLHQKIYDVVSHLIKNGKLANPV
TIKPFIPAEEKIGDITVFNYVIRLAKEAVTIINAEDYGRVIYDLFIRRSLINIGNQVVNTAFDAPLELTPSQQIETVENQ
LFELAEKGKYGGGFENFNEAIKKAIDMATAAKKRSSHLSGIATHIKTLDEKMGGLQPSDLIILAGRPGMGKTSLATNIAF
NIANACNHDANTQENEGGIVGFFSLEMSSEQLATRIISEQTEVSSSDIRRGSILDEQFSKIIRAMNQLQKAPLYIDQTGG
ISIAQLAARARRLKRQHGLDVLIIDYIQLMTSNSKRSSENRVQEITEITTGLKALAKELNIPIIALSQLSRQVENRTDKR
PQLSDLRESGSIEQDADIVLFVYREEYYLKNGEPKIGTLEYERWQETMDKAFGKAEVIIAKQRHGPTGTVHLAFQSDFTR
FSDLADSNYLPEQYG

Sequences:

>Translated_495_residues
MEETSVVNFSSSQKEEASFRELPHNIEAEQALLGALLINNDALDRVSDFLKPEHFFEPLHQKIYDVVSHLIKNGKLANPV
TIKPFIPAEEKIGDITVFNYVIRLAKEAVTIINAEDYGRVIYDLFIRRSLINIGNQVVNTAFDAPLELTPSQQIETVENQ
LFELAEKGKYGGGFENFNEAIKKAIDMATAAKKRSSHLSGIATHIKTLDEKMGGLQPSDLIILAGRPGMGKTSLATNIAF
NIANACNHDANTQENEGGIVGFFSLEMSSEQLATRIISEQTEVSSSDIRRGSILDEQFSKIIRAMNQLQKAPLYIDQTGG
ISIAQLAARARRLKRQHGLDVLIIDYIQLMTSNSKRSSENRVQEITEITTGLKALAKELNIPIIALSQLSRQVENRTDKR
PQLSDLRESGSIEQDADIVLFVYREEYYLKNGEPKIGTLEYERWQETMDKAFGKAEVIIAKQRHGPTGTVHLAFQSDFTR
FSDLADSNYLPEQYG
>Mature_495_residues
MEETSVVNFSSSQKEEASFRELPHNIEAEQALLGALLINNDALDRVSDFLKPEHFFEPLHQKIYDVVSHLIKNGKLANPV
TIKPFIPAEEKIGDITVFNYVIRLAKEAVTIINAEDYGRVIYDLFIRRSLINIGNQVVNTAFDAPLELTPSQQIETVENQ
LFELAEKGKYGGGFENFNEAIKKAIDMATAAKKRSSHLSGIATHIKTLDEKMGGLQPSDLIILAGRPGMGKTSLATNIAF
NIANACNHDANTQENEGGIVGFFSLEMSSEQLATRIISEQTEVSSSDIRRGSILDEQFSKIIRAMNQLQKAPLYIDQTGG
ISIAQLAARARRLKRQHGLDVLIIDYIQLMTSNSKRSSENRVQEITEITTGLKALAKELNIPIIALSQLSRQVENRTDKR
PQLSDLRESGSIEQDADIVLFVYREEYYLKNGEPKIGTLEYERWQETMDKAFGKAEVIIAKQRHGPTGTVHLAFQSDFTR
FSDLADSNYLPEQYG

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=483, Percent_Identity=36.0248447204969, Blast_Score=293, Evalue=2e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 55336; Mature: 55336

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEETSVVNFSSSQKEEASFRELPHNIEAEQALLGALLINNDALDRVSDFLKPEHFFEPLH
CCCCCEECCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCHHHHHHHHH
QKIYDVVSHLIKNGKLANPVTIKPFIPAEEKIGDITVFNYVIRLAKEAVTIINAEDYGRV
HHHHHHHHHHHHCCCCCCCEEECCCCCCHHHCCCHHHHHHHHHHHHHHHHEECCHHHHHH
IYDLFIRRSLINIGNQVVNTAFDAPLELTPSQQIETVENQLFELAEKGKYGGGFENFNEA
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
IKKAIDMATAAKKRSSHLSGIATHIKTLDEKMGGLQPSDLIILAGRPGMGKTSLATNIAF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHH
NIANACNHDANTQENEGGIVGFFSLEMSSEQLATRIISEQTEVSSSDIRRGSILDEQFSK
HHHHHCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHH
IIRAMNQLQKAPLYIDQTGGISIAQLAARARRLKRQHGLDVLIIDYIQLMTSNSKRSSEN
HHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHH
RVQEITEITTGLKALAKELNIPIIALSQLSRQVENRTDKRPQLSDLRESGSIEQDADIVL
HHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCEEE
FVYREEYYLKNGEPKIGTLEYERWQETMDKAFGKAEVIIAKQRHGPTGTVHLAFQSDFTR
EEEECHHHCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCEEEEEECCCHHH
FSDLADSNYLPEQYG
HHHHHCCCCCCCCCC
>Mature Secondary Structure
MEETSVVNFSSSQKEEASFRELPHNIEAEQALLGALLINNDALDRVSDFLKPEHFFEPLH
CCCCCEECCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCHHHHHHHHH
QKIYDVVSHLIKNGKLANPVTIKPFIPAEEKIGDITVFNYVIRLAKEAVTIINAEDYGRV
HHHHHHHHHHHHCCCCCCCEEECCCCCCHHHCCCHHHHHHHHHHHHHHHHEECCHHHHHH
IYDLFIRRSLINIGNQVVNTAFDAPLELTPSQQIETVENQLFELAEKGKYGGGFENFNEA
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
IKKAIDMATAAKKRSSHLSGIATHIKTLDEKMGGLQPSDLIILAGRPGMGKTSLATNIAF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHH
NIANACNHDANTQENEGGIVGFFSLEMSSEQLATRIISEQTEVSSSDIRRGSILDEQFSK
HHHHHCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHH
IIRAMNQLQKAPLYIDQTGGISIAQLAARARRLKRQHGLDVLIIDYIQLMTSNSKRSSEN
HHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHH
RVQEITEITTGLKALAKELNIPIIALSQLSRQVENRTDKRPQLSDLRESGSIEQDADIVL
HHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCEEE
FVYREEYYLKNGEPKIGTLEYERWQETMDKAFGKAEVIIAKQRHGPTGTVHLAFQSDFTR
EEEECHHHCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCEEEEEECCCHHH
FSDLADSNYLPEQYG
HHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA