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The map label for this gene is slt [H]

Identifier: 49475294

GI number: 49475294

Start: 583909

End: 586011

Strand: Reverse

Name: slt [H]

Synonym: BH04990

Alternate gene names: 49475294

Gene position: 586011-583909 (Counterclockwise)

Preceding gene: 49475297

Following gene: 49475292

Centisome position: 30.35

GC content: 39.99

Gene sequence:

>2103_bases
ATGTATGCATTCTTCATCTTCTCCTTCGTATCAACCTTTACTGCACTTATACTTGAAGCAACAATTTTATTTTCAAGTGC
CTGTGCACAAACAACTCTTTTGCACAGACAAATACCAATCCCTTTAGTTCGCCCTACCCCCTTTGTCATAAAAAAACAAG
CTACAAAACCTTTTAGACAGATTCACACAACACCCCACAATATTCCTACATTCACAAACACTTCTACGCTCAAAAAACTT
AAAGCTGGCTTAGATGCACTTTCAAATAATAATATTGCAAAAACAATCAGCCTTCGCAATTCAATGGAAAAGGATAGCCT
TGATCGCCATATTTTGACATGGGCAATTGGGATATCAAGCAAAACCAATGTACCAAGCTCGGAAATATTTAATGCAATCA
AAGAACTAAAAGGATGGCCCGGCATAGCGGCTATGCAACGCAATGCTGAACGTGCTTTTATTAATGAAACGAATTTCGCA
CCGGTGATTATCCAAAAATTTACGCATCATTTACCCCTTACAGCACAAGGTATAGCCACATATGCTAAAGCACTTATCAC
AACTGGGCAAACCGCTCGTGCTCGACAAATCATCGCGCCATGGTGGCATAAAACGAAGCTCAATGCACAAGAAGAAGAGT
TTGTTCTTAAAAATGCAAGTGCAGCATTACAACCTATTGATCATCTGAAACGCATGCAATTTATGCTTTATGCGCATCGA
TTTGATTCAGCAAAGCGCGTTGCAAAATTAGCCCATGCTCAATCTCTTTTTAAGGCTTTTATCGCAGTCGAAAAAAGTGA
TCCTCAAGCCGCACAGAAGTTACATTCTGTAGAACGATCATGGCAAAAAAGTCCTCTCCTTCAATTTGCTTACATTCGCC
ATCTTCGTCGAACTGGACAATATAGTGAAGCCGCAACACGCATGATGAGAACACCCAAAAATATCTTACGTCTTATGAAC
CCTCATGCATTATGGAGAGAACAACGTGCCCTTTCTCGTGAAATGCTCGATTTAAACAAACCTAAAATTGCTTATAAACT
CGTTGCAACACACACTGGTATGAAAGCTTCATTGACAATCGATGCTGAATTTCATGCAGGATGGTATGCACTACGGTTTC
TTCATAATCCTCAACTGGCAATGCAGCATTTTTCGCGTATCCCTCAGTTATCTTCTGCACCTCTTTCAGCATCCCGTGGA
TACTATTGGATGGGACGAACAGCAGAGATACTCGGTGAACATAAAAATGCCCAACATTATTTTCATCGCGCAGCCCATTT
CAGTGCAACTTATTATGGTCAATTAGCAGCTTCACGACTGAATCAAAAAAAACTCGAAATCTCCTTTCCAAAACCAACAA
CCTCTGAGCGACAACGTTTTAGCGCACGAAAAGCTATTCAAGCAATTCAACATCTTGAATCAGCTGGCTATTCTGATTTT
GCTAAAATTTTTTATAGAGAACTCGGAGAAAAAATAGAAAGCCCTGGTGAATTAGCTCTTCTAGCTGTTATGGCAGAAAA
AAACGGTGACTATTATACAAGCCTTAAAATTGGAAAAACTGCTGTTTTTCAAGGTAAAAATGTTGGTGCACTCTCCCATC
CTCTCGGTGCAATACCAACCTCCGCTAATATTTCTGTAGCAGGAAAAGCGCTTATCTATGCCATTGCACGTCAAGAAAGC
GAATTTAATCCAAAAGCCATATCAAAAGCAGGAGCACAAGGAATCCTTCAATTGCTTCCTACGACAGCAAAAGCACTCGC
AAAAAAGCATTCAATCGCATGGTCACAAAAAAAATTCAGCAGTGATGCCAGTTATAATGCTACGTTAGGTGCCCATTTTC
TAAGGGAACAATTGGAACGTTTTAACGGATCCTATATATTGGCCCTTATTAGTTATAATGCAGGCCCCCGTCGCGTTAAT
GAGTGGATAAAACGCTATGGTGATCCTCGAGGACAACCTCTTGATAAAGTCATCGATTGGATTGAACGTATCCCTTATAC
AGAAACACGTAATTACGTAATGCGTATCATGGAAAATTATGGAGTCTACAAAGCAAGACTTACTGGAAAAACAGATATAA
AAGCTGATCTTCTTTCCGGTTGA

Upstream 100 bases:

>100_bases
ATTTCTTTAAAGTATCAATCTGCATTAACGCGTTTTTCATGCTTCTGTCTTAGAAAAACTTGAGTGTTTCAAAAAAGTAA
GGTCTTAAGGTCTTTTCTCT

Downstream 100 bases:

>100_bases
TGAAAATGAAAAAACTTCAATCCACTAAAAAGCAGTTCGTTCCGTTTCGTCAACTTATAGACCACAATATCATAGCAATT
CTAAACATGAAGGAATGTGA

Product: soluble lytic murein transglycosylase precursor

Products: 1,6-Anhydrobond [C]

Alternate protein names: NA

Number of amino acids: Translated: 700; Mature: 700

Protein sequence:

>700_residues
MYAFFIFSFVSTFTALILEATILFSSACAQTTLLHRQIPIPLVRPTPFVIKKQATKPFRQIHTTPHNIPTFTNTSTLKKL
KAGLDALSNNNIAKTISLRNSMEKDSLDRHILTWAIGISSKTNVPSSEIFNAIKELKGWPGIAAMQRNAERAFINETNFA
PVIIQKFTHHLPLTAQGIATYAKALITTGQTARARQIIAPWWHKTKLNAQEEEFVLKNASAALQPIDHLKRMQFMLYAHR
FDSAKRVAKLAHAQSLFKAFIAVEKSDPQAAQKLHSVERSWQKSPLLQFAYIRHLRRTGQYSEAATRMMRTPKNILRLMN
PHALWREQRALSREMLDLNKPKIAYKLVATHTGMKASLTIDAEFHAGWYALRFLHNPQLAMQHFSRIPQLSSAPLSASRG
YYWMGRTAEILGEHKNAQHYFHRAAHFSATYYGQLAASRLNQKKLEISFPKPTTSERQRFSARKAIQAIQHLESAGYSDF
AKIFYRELGEKIESPGELALLAVMAEKNGDYYTSLKIGKTAVFQGKNVGALSHPLGAIPTSANISVAGKALIYAIARQES
EFNPKAISKAGAQGILQLLPTTAKALAKKHSIAWSQKKFSSDASYNATLGAHFLREQLERFNGSYILALISYNAGPRRVN
EWIKRYGDPRGQPLDKVIDWIERIPYTETRNYVMRIMENYGVYKARLTGKTDIKADLLSG

Sequences:

>Translated_700_residues
MYAFFIFSFVSTFTALILEATILFSSACAQTTLLHRQIPIPLVRPTPFVIKKQATKPFRQIHTTPHNIPTFTNTSTLKKL
KAGLDALSNNNIAKTISLRNSMEKDSLDRHILTWAIGISSKTNVPSSEIFNAIKELKGWPGIAAMQRNAERAFINETNFA
PVIIQKFTHHLPLTAQGIATYAKALITTGQTARARQIIAPWWHKTKLNAQEEEFVLKNASAALQPIDHLKRMQFMLYAHR
FDSAKRVAKLAHAQSLFKAFIAVEKSDPQAAQKLHSVERSWQKSPLLQFAYIRHLRRTGQYSEAATRMMRTPKNILRLMN
PHALWREQRALSREMLDLNKPKIAYKLVATHTGMKASLTIDAEFHAGWYALRFLHNPQLAMQHFSRIPQLSSAPLSASRG
YYWMGRTAEILGEHKNAQHYFHRAAHFSATYYGQLAASRLNQKKLEISFPKPTTSERQRFSARKAIQAIQHLESAGYSDF
AKIFYRELGEKIESPGELALLAVMAEKNGDYYTSLKIGKTAVFQGKNVGALSHPLGAIPTSANISVAGKALIYAIARQES
EFNPKAISKAGAQGILQLLPTTAKALAKKHSIAWSQKKFSSDASYNATLGAHFLREQLERFNGSYILALISYNAGPRRVN
EWIKRYGDPRGQPLDKVIDWIERIPYTETRNYVMRIMENYGVYKARLTGKTDIKADLLSG
>Mature_700_residues
MYAFFIFSFVSTFTALILEATILFSSACAQTTLLHRQIPIPLVRPTPFVIKKQATKPFRQIHTTPHNIPTFTNTSTLKKL
KAGLDALSNNNIAKTISLRNSMEKDSLDRHILTWAIGISSKTNVPSSEIFNAIKELKGWPGIAAMQRNAERAFINETNFA
PVIIQKFTHHLPLTAQGIATYAKALITTGQTARARQIIAPWWHKTKLNAQEEEFVLKNASAALQPIDHLKRMQFMLYAHR
FDSAKRVAKLAHAQSLFKAFIAVEKSDPQAAQKLHSVERSWQKSPLLQFAYIRHLRRTGQYSEAATRMMRTPKNILRLMN
PHALWREQRALSREMLDLNKPKIAYKLVATHTGMKASLTIDAEFHAGWYALRFLHNPQLAMQHFSRIPQLSSAPLSASRG
YYWMGRTAEILGEHKNAQHYFHRAAHFSATYYGQLAASRLNQKKLEISFPKPTTSERQRFSARKAIQAIQHLESAGYSDF
AKIFYRELGEKIESPGELALLAVMAEKNGDYYTSLKIGKTAVFQGKNVGALSHPLGAIPTSANISVAGKALIYAIARQES
EFNPKAISKAGAQGILQLLPTTAKALAKKHSIAWSQKKFSSDASYNATLGAHFLREQLERFNGSYILALISYNAGPRRVN
EWIKRYGDPRGQPLDKVIDWIERIPYTETRNYVMRIMENYGVYKARLTGKTDIKADLLSG

Specific function: Murein-Degrading Enzyme. Catalyzes The Cleavage Of The Glycosidic Bonds Between N-Acetylmuramic Acid And N- Acetylglucosamine Residues In Peptidoglycan. May Play A Role In Recycling Of Muropeptides During Cell Elongation And/Or Cell Division. [C]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasmic Protein. Tightly Associated With The Murein Sacculus [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 10 TPR repeats [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=132, Percent_Identity=37.1212121212121, Blast_Score=80, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011055
- InterPro:   IPR008258
- InterPro:   IPR016047
- InterPro:   IPR010090
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01551 Peptidase_M23; PF10145 PhageMin_Tail; PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 78950; Mature: 78950

Theoretical pI: Translated: 10.71; Mature: 10.71

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYAFFIFSFVSTFTALILEATILFSSACAQTTLLHRQIPIPLVRPTPFVIKKQATKPFRQ
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHEECCCCHHHHH
IHTTPHNIPTFTNTSTLKKLKAGLDALSNNNIAKTISLRNSMEKDSLDRHILTWAIGISS
HCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEHHHHHHCCCHHHHHHEEEEEEEECCC
KTNVPSSEIFNAIKELKGWPGIAAMQRNAERAFINETNFAPVIIQKFTHHLPLTAQGIAT
CCCCCHHHHHHHHHHHCCCCCHHHHHCCCCHHCCCCCCCCHHHHHHHHHCCCCCHHHHHH
YAKALITTGQTARARQIIAPWWHKTKLNAQEEEFVLKNASAALQPIDHLKRMQFMLYAHR
HHHHHHCCCCCHHHHHHHCCCHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
FDSAKRVAKLAHAQSLFKAFIAVEKSDPQAAQKLHSVERSWQKSPLLQFAYIRHLRRTGQ
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCC
YSEAATRMMRTPKNILRLMNPHALWREQRALSREMLDLNKPKIAYKLVATHTGMKASLTI
HHHHHHHHHHCHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCEEEEEE
DAEFHAGWYALRFLHNPQLAMQHFSRIPQLSSAPLSASRGYYWMGRTAEILGEHKNAQHY
EECCCCCHHEEHHHCCHHHHHHHHHHCCCCCCCCCCCCCCEEEECCHHHHHCCCCCHHHH
FHRAAHFSATYYGQLAASRLNQKKLEISFPKPTTSERQRFSARKAIQAIQHLESAGYSDF
HHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHH
AKIFYRELGEKIESPGELALLAVMAEKNGDYYTSLKIGKTAVFQGKNVGALSHPLGAIPT
HHHHHHHHHHHHCCCCCEEEEEEEECCCCCEEEEEEECCEEEECCCCCCCCCCCCCCCCC
SANISVAGKALIYAIARQESEFNPKAISKAGAQGILQLLPTTAKALAKKHSIAWSQKKFS
CCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SDASYNATLGAHFLREQLERFNGSYILALISYNAGPRRVNEWIKRYGDPRGQPLDKVIDW
CCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCHHHHHHH
IERIPYTETRNYVMRIMENYGVYKARLTGKTDIKADLLSG
HHHCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCHHHCCC
>Mature Secondary Structure
MYAFFIFSFVSTFTALILEATILFSSACAQTTLLHRQIPIPLVRPTPFVIKKQATKPFRQ
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHEECCCCHHHHH
IHTTPHNIPTFTNTSTLKKLKAGLDALSNNNIAKTISLRNSMEKDSLDRHILTWAIGISS
HCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEHHHHHHCCCHHHHHHEEEEEEEECCC
KTNVPSSEIFNAIKELKGWPGIAAMQRNAERAFINETNFAPVIIQKFTHHLPLTAQGIAT
CCCCCHHHHHHHHHHHCCCCCHHHHHCCCCHHCCCCCCCCHHHHHHHHHCCCCCHHHHHH
YAKALITTGQTARARQIIAPWWHKTKLNAQEEEFVLKNASAALQPIDHLKRMQFMLYAHR
HHHHHHCCCCCHHHHHHHCCCHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
FDSAKRVAKLAHAQSLFKAFIAVEKSDPQAAQKLHSVERSWQKSPLLQFAYIRHLRRTGQ
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCC
YSEAATRMMRTPKNILRLMNPHALWREQRALSREMLDLNKPKIAYKLVATHTGMKASLTI
HHHHHHHHHHCHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCEEEEEE
DAEFHAGWYALRFLHNPQLAMQHFSRIPQLSSAPLSASRGYYWMGRTAEILGEHKNAQHY
EECCCCCHHEEHHHCCHHHHHHHHHHCCCCCCCCCCCCCCEEEECCHHHHHCCCCCHHHH
FHRAAHFSATYYGQLAASRLNQKKLEISFPKPTTSERQRFSARKAIQAIQHLESAGYSDF
HHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHH
AKIFYRELGEKIESPGELALLAVMAEKNGDYYTSLKIGKTAVFQGKNVGALSHPLGAIPT
HHHHHHHHHHHHCCCCCEEEEEEEECCCCCEEEEEEECCEEEECCCCCCCCCCCCCCCCC
SANISVAGKALIYAIARQESEFNPKAISKAGAQGILQLLPTTAKALAKKHSIAWSQKKFS
CCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SDASYNATLGAHFLREQLERFNGSYILALISYNAGPRRVNEWIKRYGDPRGQPLDKVIDW
CCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCHHHHHHH
IERIPYTETRNYVMRIMENYGVYKARLTGKTDIKADLLSG
HHHCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]