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The map label for this gene is truB

Identifier: 49475025

GI number: 49475025

Start: 288158

End: 289117

Strand: Reverse

Name: truB

Synonym: BH02130

Alternate gene names: 49475025

Gene position: 289117-288158 (Counterclockwise)

Preceding gene: 49475026

Following gene: 49475023

Centisome position: 14.97

GC content: 40.73

Gene sequence:

>960_bases
ATGGCACGGCAAAGCAAAAAAAAAGGCCGTCCTGTTTCTGGTTGGATTATACTTGATAAACCAAAAGGTATGAGATCAAC
AGAAGCTGTCTCACAAATTAAATACCTCTTTCATGCGCAAAAAGTAGGACACGCCGGCACGCTTGATCCCCTTGCTTCTG
GTCTCCTGCCAATTGCATTGGGGGAAGCAACCAAAACCGTTCCCTACGTGATGCAAGGTACAAAAACCTACCGTTTTCAC
ATCGCTTGGGGAGAAGAGCGTTCAACAGATGATTTAGAGGGTGAAATAACGCACACATCTTCTAAACGTCCAACACGAGA
AGAGATACTTGCTCTTCTTCCTCAATATACAGGTGTTATTTTGCAAACACCCCCACAATTTTCGGCAATTAAAATTGCCG
GTAATCGAGCCTATGATCTAGCACGTGAAGGTGAAGTCGTTGAAATCCCACCACGTCAAGTGGAAATAGAAACTTTCAAA
TTAATAGAAATGATAACCAAAGATCATTCTATCTTTGAAATCACCTGTGGAAAAGGAACCTATGTGCGCTCCTTAGCACG
CGATATGGGACGTGATCTTGGTTGTTATGCGCATATTGCTGATTTAAGACGTATTGCAGTCGCACCTTTTTGTGAAAATG
ATCTCATCACATGGGATGAACTCAAAGCAGTTGAACTAGATAAAAGTGCAAAAAATGAAAAGGATGCTCCTTTTGAAAGA
AATTTCATAAAACTTGACGAACTCTTAATTGAAACGATTTCTGCACTAGAATGTCTCTCTCATTATACCCTTAGCCAAAC
TCAAGCACAGCAAGTTATGAAAGGCAATACAGTGCTCCTATATCATCACGATGTACCTCTTGATGAAGATGAGGTTTGTG
TCCTGTATCAAGACCAACTTCTTGCTATAGGCGCTCTTGAAAAAAACCAATTCAAGCCAAAGCGTCTTTTCACAATTTAA

Upstream 100 bases:

>100_bases
GATAACAGTTTTGATAATTTTTCAAAAATAGATGCTCTCCTGCGCTCACCTGAAGTAGCACGCGATCTTCACCATAACGA
TAAAGTTGGAGATTAAAGAT

Downstream 100 bases:

>100_bases
TCCGCTTTTTCTCACAGCAATTTCTTACAGAAAAATACTTTTCATATTAATAAAGGGAAGAAAGCTCTTTTCACTGTTTT
AAACTTACCTTTTCACGATA

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MARQSKKKGRPVSGWIILDKPKGMRSTEAVSQIKYLFHAQKVGHAGTLDPLASGLLPIALGEATKTVPYVMQGTKTYRFH
IAWGEERSTDDLEGEITHTSSKRPTREEILALLPQYTGVILQTPPQFSAIKIAGNRAYDLAREGEVVEIPPRQVEIETFK
LIEMITKDHSIFEITCGKGTYVRSLARDMGRDLGCYAHIADLRRIAVAPFCENDLITWDELKAVELDKSAKNEKDAPFER
NFIKLDELLIETISALECLSHYTLSQTQAQQVMKGNTVLLYHHDVPLDEDEVCVLYQDQLLAIGALEKNQFKPKRLFTI

Sequences:

>Translated_319_residues
MARQSKKKGRPVSGWIILDKPKGMRSTEAVSQIKYLFHAQKVGHAGTLDPLASGLLPIALGEATKTVPYVMQGTKTYRFH
IAWGEERSTDDLEGEITHTSSKRPTREEILALLPQYTGVILQTPPQFSAIKIAGNRAYDLAREGEVVEIPPRQVEIETFK
LIEMITKDHSIFEITCGKGTYVRSLARDMGRDLGCYAHIADLRRIAVAPFCENDLITWDELKAVELDKSAKNEKDAPFER
NFIKLDELLIETISALECLSHYTLSQTQAQQVMKGNTVLLYHHDVPLDEDEVCVLYQDQLLAIGALEKNQFKPKRLFTI
>Mature_318_residues
ARQSKKKGRPVSGWIILDKPKGMRSTEAVSQIKYLFHAQKVGHAGTLDPLASGLLPIALGEATKTVPYVMQGTKTYRFHI
AWGEERSTDDLEGEITHTSSKRPTREEILALLPQYTGVILQTPPQFSAIKIAGNRAYDLAREGEVVEIPPRQVEIETFKL
IEMITKDHSIFEITCGKGTYVRSLARDMGRDLGCYAHIADLRRIAVAPFCENDLITWDELKAVELDKSAKNEKDAPFERN
FIKLDELLIETISALECLSHYTLSQTQAQQVMKGNTVLLYHHDVPLDEDEVCVLYQDQLLAIGALEKNQFKPKRLFTI

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=217, Percent_Identity=32.7188940092166, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI4503337, Length=244, Percent_Identity=29.0983606557377, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI215599015, Length=244, Percent_Identity=29.0983606557377, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI2367200, Length=276, Percent_Identity=34.7826086956522, Blast_Score=166, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI17553978, Length=244, Percent_Identity=28.6885245901639, Blast_Score=81, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6323204, Length=248, Percent_Identity=29.0322580645161, Blast_Score=87, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6324037, Length=134, Percent_Identity=37.3134328358209, Blast_Score=84, Evalue=2e-17,
Organism=Drosophila melanogaster, GI281364189, Length=246, Percent_Identity=28.4552845528455, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI281364187, Length=246, Percent_Identity=28.4552845528455, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI281364185, Length=246, Percent_Identity=28.4552845528455, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI281364183, Length=246, Percent_Identity=28.4552845528455, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI62471759, Length=246, Percent_Identity=28.4552845528455, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI17975520, Length=246, Percent_Identity=28.4552845528455, Blast_Score=79, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_BARHE (Q6G5F5)

Other databases:

- EMBL:   BX897699
- RefSeq:   YP_033066.1
- ProteinModelPortal:   Q6G5F5
- SMR:   Q6G5F5
- GeneID:   2865244
- GenomeReviews:   BX897699_GR
- KEGG:   bhe:BH02130
- NMPDR:   fig|283166.1.peg.194
- HOGENOM:   HBG397258
- OMA:   ASTKAYD
- PhylomeDB:   Q6G5F5
- ProtClustDB:   PRK05389
- BioCyc:   BHEN283166:BH02130-MONOMER
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780
- InterPro:   IPR015240
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF09157 TruB-C_2; PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom

EC number: 4.2.1.70

Molecular weight: Translated: 35953; Mature: 35822

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: NA

Important sites: ACT_SITE 49-49

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARQSKKKGRPVSGWIILDKPKGMRSTEAVSQIKYLFHAQKVGHAGTLDPLASGLLPIAL
CCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCEEEEE
GEATKTVPYVMQGTKTYRFHIAWGEERSTDDLEGEITHTSSKRPTREEILALLPQYTGVI
CCCCCCCCCEEECCEEEEEEEEECCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHCCEE
LQTPPQFSAIKIAGNRAYDLAREGEVVEIPPRQVEIETFKLIEMITKDHSIFEITCGKGT
EECCCCCEEEEEECCCEEHHHCCCCEEECCCCEEEHHHHHHHHHHHCCCCEEEEECCCCH
YVRSLARDMGRDLGCYAHIADLRRIAVAPFCENDLITWDELKAVELDKSAKNEKDAPFER
HHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHCEEEECCCCCCCCCCCCCCC
NFIKLDELLIETISALECLSHYTLSQTQAQQVMKGNTVLLYHHDVPLDEDEVCVLYQDQL
CHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCEEEEEECCCCCCCCCEEEEEECCE
LAIGALEKNQFKPKRLFTI
EEEECCCCCCCCCCCCCCC
>Mature Secondary Structure 
ARQSKKKGRPVSGWIILDKPKGMRSTEAVSQIKYLFHAQKVGHAGTLDPLASGLLPIAL
CCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCEEEEE
GEATKTVPYVMQGTKTYRFHIAWGEERSTDDLEGEITHTSSKRPTREEILALLPQYTGVI
CCCCCCCCCEEECCEEEEEEEEECCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHCCEE
LQTPPQFSAIKIAGNRAYDLAREGEVVEIPPRQVEIETFKLIEMITKDHSIFEITCGKGT
EECCCCCEEEEEECCCEEHHHCCCCEEECCCCEEEHHHHHHHHHHHCCCCEEEEECCCCH
YVRSLARDMGRDLGCYAHIADLRRIAVAPFCENDLITWDELKAVELDKSAKNEKDAPFER
HHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHCEEEECCCCCCCCCCCCCCC
NFIKLDELLIETISALECLSHYTLSQTQAQQVMKGNTVLLYHHDVPLDEDEVCVLYQDQL
CHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCEEEEEECCCCCCCCCEEEEEECCE
LAIGALEKNQFKPKRLFTI
EEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA