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The map label for this gene is ansA [H]

Identifier: 49475018

GI number: 49475018

Start: 278829

End: 279818

Strand: Reverse

Name: ansA [H]

Synonym: BH02060

Alternate gene names: 49475018

Gene position: 279818-278829 (Counterclockwise)

Preceding gene: 49475021

Following gene: 49475017

Centisome position: 14.49

GC content: 39.29

Gene sequence:

>990_bases
ATGAAAAAAATAGCCATAGGGACATTGGGTGGAACAATTGCAATGGCTGCTGACAATTTGGGTCAAATGCAACCGATTTT
AACTTCAGATATACTGATCAAAAGTGTTCCTGATTTAGATAGCGTTGCTGATATTCATGCTCAAACATTAATACAAATAC
CAAGTGGCTCTTTATCTTTCAAAAGTCTTTTTGAAATTATAGAGTGGGCAACACAACAAATAAAAGCGGGGGCAGAAGGT
ATTGTTTTAACCCAAGGTACCGATACAGTGGAAGAAACAGCTTTTTTTCTTTCCCTCTACTGGAATAAAGCCGAACCACT
TATTGTAACTGGTGCTATGCGTCTACCTCATGAAGCAGGTGCTGATGGACCAGCTAATATTTTGGCCGCAACCCGTGTCG
CTGCTCATCCACAAAGTCGAAATAGAGGTGTTTTAGTTGTAATCAACGATACAATTCATTCACCTTATTGGGTCCAGAAA
AGCCATACCATCAAAATTGAGACATTTCAGTCAAGTCCGGTAGGTGTTCTAGGCATTATTTTGGAAGGAAAACTGATTTA
TTTTACTGATCGAAATTTTTTTCCAACAACTTTTGATCTTCCCAAAAACTATGATCACCAAGTTGCAATTCTATACTCTT
CTCTATCCTCTGATACACAGTTAATGAAATTCTGCTTTGAAAGTGGCCATTATGCTGGAGTTGTTATTGCAGGTTTTGGA
TCAGGACACTGCAGCTTTGAAGAAGCAGATATCGTGCGACAATATACACAAAAAATGCCAATCATTATCGCTAGCCGTAC
CTACACTGGCCCTACAACCCGCAAAACTTATGGCTATAAAGGTTCAGAAGTCGATATGATTGCTTCTGGCGCCCTCATGT
CTGGTTATTTATCAGCACTAAAAGCACGTTTACTTTTATGGGCTTTTTTGGCACAAGGGCTCTCATTGGAACAAATTAAT
GCACATTGGGATGACTGGACTATAAGCTAA

Upstream 100 bases:

>100_bases
ATTCAATTTATTGGAATAAAAAAAAGATATCGTTGTTGTCAATGCAAATTGTTGCTAAATAACTTTATGTAAATAGTTTA
CGCCTTTTAGAGGAAAAATC

Downstream 100 bases:

>100_bases
AGATTATCTATCTTAAAGAAGAAAACATGCAAAATAAAGTTCATTTAAATACAAAAGGGCCTGCCTGTCTATCCTTAATC
AATTACTTCGGCAAAAAAAT

Product: L-asparaginase

Products: NA

Alternate protein names: L-ASNase; L-asparagine amidohydrolase [H]

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MKKIAIGTLGGTIAMAADNLGQMQPILTSDILIKSVPDLDSVADIHAQTLIQIPSGSLSFKSLFEIIEWATQQIKAGAEG
IVLTQGTDTVEETAFFLSLYWNKAEPLIVTGAMRLPHEAGADGPANILAATRVAAHPQSRNRGVLVVINDTIHSPYWVQK
SHTIKIETFQSSPVGVLGIILEGKLIYFTDRNFFPTTFDLPKNYDHQVAILYSSLSSDTQLMKFCFESGHYAGVVIAGFG
SGHCSFEEADIVRQYTQKMPIIIASRTYTGPTTRKTYGYKGSEVDMIASGALMSGYLSALKARLLLWAFLAQGLSLEQIN
AHWDDWTIS

Sequences:

>Translated_329_residues
MKKIAIGTLGGTIAMAADNLGQMQPILTSDILIKSVPDLDSVADIHAQTLIQIPSGSLSFKSLFEIIEWATQQIKAGAEG
IVLTQGTDTVEETAFFLSLYWNKAEPLIVTGAMRLPHEAGADGPANILAATRVAAHPQSRNRGVLVVINDTIHSPYWVQK
SHTIKIETFQSSPVGVLGIILEGKLIYFTDRNFFPTTFDLPKNYDHQVAILYSSLSSDTQLMKFCFESGHYAGVVIAGFG
SGHCSFEEADIVRQYTQKMPIIIASRTYTGPTTRKTYGYKGSEVDMIASGALMSGYLSALKARLLLWAFLAQGLSLEQIN
AHWDDWTIS
>Mature_329_residues
MKKIAIGTLGGTIAMAADNLGQMQPILTSDILIKSVPDLDSVADIHAQTLIQIPSGSLSFKSLFEIIEWATQQIKAGAEG
IVLTQGTDTVEETAFFLSLYWNKAEPLIVTGAMRLPHEAGADGPANILAATRVAAHPQSRNRGVLVVINDTIHSPYWVQK
SHTIKIETFQSSPVGVLGIILEGKLIYFTDRNFFPTTFDLPKNYDHQVAILYSSLSSDTQLMKFCFESGHYAGVVIAGFG
SGHCSFEEADIVRQYTQKMPIIIASRTYTGPTTRKTYGYKGSEVDMIASGALMSGYLSALKARLLLWAFLAQGLSLEQIN
AHWDDWTIS

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1789327, Length=300, Percent_Identity=32.6666666666667, Blast_Score=134, Evalue=7e-33,
Organism=Saccharomyces cerevisiae, GI6320527, Length=334, Percent_Identity=30.5389221556886, Blast_Score=137, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6323189, Length=315, Percent_Identity=29.8412698412698, Blast_Score=124, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6323187, Length=315, Percent_Identity=29.8412698412698, Blast_Score=124, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6323186, Length=315, Percent_Identity=29.8412698412698, Blast_Score=124, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6323184, Length=315, Percent_Identity=29.8412698412698, Blast_Score=124, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004550
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 35951; Mature: 35951

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIAIGTLGGTIAMAADNLGQMQPILTSDILIKSVPDLDSVADIHAQTLIQIPSGSLSF
CCEEEEEECCCEEEEECCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHEEEECCCCCCCH
KSLFEIIEWATQQIKAGAEGIVLTQGTDTVEETAFFLSLYWNKAEPLIVTGAMRLPHEAG
HHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHEEEEEEEECCCCCEEEECCEECCHHCC
ADGPANILAATRVAAHPQSRNRGVLVVINDTIHSPYWVQKSHTIKIETFQSSPVGVLGII
CCCCHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCEEECCCCEEEEEEECCCCCEEEEEE
LEGKLIYFTDRNFFPTTFDLPKNYDHQVAILYSSLSSDTQLMKFCFESGHYAGVVIAGFG
EECEEEEEECCCCCCCEECCCCCCCCEEEEEEECCCCCHHHHHHHHHCCCEEEEEEEECC
SGHCSFEEADIVRQYTQKMPIIIASRTYTGPTTRKTYGYKGSEVDMIASGALMSGYLSAL
CCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHH
KARLLLWAFLAQGLSLEQINAHWDDWTIS
HHHHHHHHHHHCCCCEEHHCCCCCCCCCC
>Mature Secondary Structure
MKKIAIGTLGGTIAMAADNLGQMQPILTSDILIKSVPDLDSVADIHAQTLIQIPSGSLSF
CCEEEEEECCCEEEEECCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHEEEECCCCCCCH
KSLFEIIEWATQQIKAGAEGIVLTQGTDTVEETAFFLSLYWNKAEPLIVTGAMRLPHEAG
HHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHEEEEEEEECCCCCEEEECCEECCHHCC
ADGPANILAATRVAAHPQSRNRGVLVVINDTIHSPYWVQKSHTIKIETFQSSPVGVLGII
CCCCHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCEEECCCCEEEEEEECCCCCEEEEEE
LEGKLIYFTDRNFFPTTFDLPKNYDHQVAILYSSLSSDTQLMKFCFESGHYAGVVIAGFG
EECEEEEEECCCCCCCEECCCCCCCCEEEEEEECCCCCHHHHHHHHHCCCEEEEEEEECC
SGHCSFEEADIVRQYTQKMPIIIASRTYTGPTTRKTYGYKGSEVDMIASGALMSGYLSAL
CCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHH
KARLLLWAFLAQGLSLEQINAHWDDWTIS
HHHHHHHHHHHCCCCEEHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]